Q9VJ33 (NEDD8_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD8

Alternative name(s):
Neddylin
Ubiquitin-like protein Nedd8

Gene names

Name:	Nedd8
ORF Names:	CG10679

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin-like protein which plays an important role in cell cycle control, embryogenesis and neurogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex Uba3-Ula1 and linkage to the E2 enzyme Ubc12. Attachment of Nedd8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Ref.4 Ref.5 Ref.6
Subunit structure	Covalently attached to cullins. Ref.4 Ref.5 Ref.6
Subcellular location	Nucleus. Note: Mainly nuclear By similarity.
Post-translational modification	Cleavage of precursor form is necessary for function By similarity.
Disruption phenotype	Flies die from growth arrest in the first-instar larval stage. Ref.4
Sequence similarities	Belongs to the ubiquitin family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Developmental protein
PTM	Isopeptide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell proliferation Inferred from mutant phenotype Ref.4. Source: UniProtKB multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein neddylation Inferred from mutant phenotype. Source: FlyBase regulation of protein stability Inferred from mutant phenotype Ref.4. Source: UniProtKB regulation of proteolysis Inferred from mutant phenotype Ref.4. Source: UniProtKB regulation of smoothened signaling pathway Inferred from mutant phenotype Ref.4. Source: UniProtKB regulation of ubiquitin-protein ligase activity Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein tag Non-traceable author statement Ref.5Ref.6. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 76	76	NEDD8		PRO_0000042775
	Propeptide	77 – 84	8	By similarity		PRO_0000042776
Regions
	Region	70 – 72	3	Interaction with Uba3 By similarity
Sites
	Site	8	1	Interaction with Uba3 By similarity
	Site	44	1	Interaction with Uba3 By similarity
Amino acid modifications
	Cross-link	76		Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Sequences

	Sequence		Length	Mass (Da)
	Q9VJ33 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2B0858BB4DC77AA3	FASTA	84	9,346
	10 20 30 40 50 60 MLIKVKTLTG KEIEIDIEPT DKVDRIKERV EEKEGIPPQQ QRLIFSGKQM NDDKTAADYK 70 80 VQGGSVLHLV LALRGGDSIL TPCV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Distinct protein degradation mechanisms mediated by Cul1 and Cul3 controlling Ci stability in Drosophila eye development." Ou C.-Y., Lin Y.-F., Chen Y.-J., Chien C.-T. Genes Dev. 16:2403-2414(2002) [PubMed: 12231629] [Abstract] Cited for: FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE.
[5]	"Requirement of Cul3 for axonal arborization and dendritic elaboration in Drosophila mushroom body neurons." Zhu S., Perez R., Pan M., Lee T. J. Neurosci. 25:4189-4197(2005) [PubMed: 15843622] [Abstract] Cited for: FUNCTION, SUBUNIT.
[6]	"Neddylation and deneddylation regulate Cul1 and Cul3 protein accumulation." Wu J.-T., Lin H.-C., Hu Y.-C., Chien C.-T. Nat. Cell Biol. 7:1014-1020(2005) [PubMed: 16127432] [Abstract] Cited for: FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAF53724.1. BT024445 mRNA. Translation: ABC86507.1.
RefSeq	NP_609919.1. NM_136075.2.
3D structure databases
ProteinModelPortal	Q9VJ33.
SMR	Q9VJ33. Positions 1-76.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VJ33. 7 interactions.
MINT	MINT-912533.
STRING	Q9VJ33.
Proteomic databases
PRIDE	Q9VJ33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081192; FBpp0080733; FBgn0032725.
GeneID	35151.
KEGG	dme:Dmel_CG10679.
NMPDR	fig|7227.3.peg.2892.
Organism-specific databases
CTD	4738.
FlyBase	FBgn0032725. Nedd8.
Phylogenomic databases
eggNOG	inNOG11027.
GeneTree	EMGT00050000005052.
InParanoid	Q9VJ33.
OMA	DYKVQGG.
OrthoDB	EOG4HMGT4.
PhylomeDB	Q9VJ33.
Gene expression databases
Bgee	Q9VJ33.
GermOnline	CG10679. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000626. Ubiquitin. IPR019954. Ubiquitin_CS. IPR019956. Ubiquitin_subgr. IPR019955. Ubiquitin_supergroup. [Graphical view]
KO	K12158.
Pfam	PF00240. ubiquitin. 1 hit. [Graphical view]
PRINTS	PR00348. UBIQUITIN.
SMART	SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS00299. UBIQUITIN_1. 1 hit. PS50053. UBIQUITIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	792117.

Entry information

Entry name

NEDD8_DROME

Accession

Primary (citable) accession number: Q9VJ33
Secondary accession number(s): Q29QE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project