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Protein

NEDD8

Gene

Nedd8

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein which plays an important role in cell cycle control, embryogenesis and neurogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex Uba3-Ula1 and linkage to the E2 enzyme Ubc12. Attachment of Nedd8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.3 Publications

GO - Molecular functioni

  • protein tag Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: UniProtKB
  • female germ-line stem cell population maintenance Source: FlyBase
  • neurogenesis Source: FlyBase
  • neuron projection morphogenesis Source: FlyBase
  • protein neddylation Source: FlyBase
  • regulation of protein stability Source: UniProtKB
  • regulation of proteolysis Source: UniProtKB
  • regulation of smoothened signaling pathway Source: UniProtKB
  • regulation of ubiquitin-protein transferase activity Source: UniProtKB
  • sleep Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8
Alternative name(s):
Neddylin
Ubiquitin-like protein Nedd8
Gene namesi
Name:Nedd8
ORF Names:CG10679
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0032725. Nedd8.

Subcellular locationi

  • Nucleus

  • Note: Mainly nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Flies die from growth arrest in the first-instar larval stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676NEDD8PRO_0000042775Add
BLAST
Propeptidei77 – 848By similarityPRO_0000042776

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Post-translational modificationi

Cleavage of precursor form is necessary for function.By similarity

Keywords - PTMi

Isopeptide bond

Proteomic databases

PaxDbiQ9VJ33.
PRIDEiQ9VJ33.

Expressioni

Gene expression databases

BgeeiFBgn0032725.
ExpressionAtlasiQ9VJ33. differential.
GenevisibleiQ9VJ33. DM.

Interactioni

Subunit structurei

Covalently attached to cullins.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei8 – 81Interaction with Uba3By similarity
Sitei44 – 441Interaction with Uba3By similarity

Protein-protein interaction databases

BioGridi61142. 6 interactions.
IntActiQ9VJ33. 8 interactions.
MINTiMINT-912533.
STRINGi7227.FBpp0080733.

Structurei

3D structure databases

ProteinModelPortaliQ9VJ33.
SMRiQ9VJ33. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 723Interaction with Uba3By similarity

Sequence similaritiesi

Belongs to the ubiquitin family.Curated

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
InParanoidiQ9VJ33.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG091G178I.
PhylomeDBiQ9VJ33.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VJ33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIKVKTLTG KEIEIDIEPT DKVDRIKERV EEKEGIPPQQ QRLIFSGKQM
60 70 80
NDDKTAADYK VQGGSVLHLV LALRGGDSIL TPCV
Length:84
Mass (Da):9,346
Last modified:May 1, 2000 - v1
Checksum:i2B0858BB4DC77AA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF53724.1.
BT024445 mRNA. Translation: ABC86507.1.
RefSeqiNP_001286070.1. NM_001299141.1.
NP_609919.1. NM_136075.3.
UniGeneiDm.30848.

Genome annotation databases

EnsemblMetazoaiFBtr0081192; FBpp0080733; FBgn0032725.
FBtr0342928; FBpp0309711; FBgn0032725.
GeneIDi35151.
KEGGidme:Dmel_CG10679.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014134 Genomic DNA. Translation: AAF53724.1.
BT024445 mRNA. Translation: ABC86507.1.
RefSeqiNP_001286070.1. NM_001299141.1.
NP_609919.1. NM_136075.3.
UniGeneiDm.30848.

3D structure databases

ProteinModelPortaliQ9VJ33.
SMRiQ9VJ33. Positions 1-76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61142. 6 interactions.
IntActiQ9VJ33. 8 interactions.
MINTiMINT-912533.
STRINGi7227.FBpp0080733.

Proteomic databases

PaxDbiQ9VJ33.
PRIDEiQ9VJ33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081192; FBpp0080733; FBgn0032725.
FBtr0342928; FBpp0309711; FBgn0032725.
GeneIDi35151.
KEGGidme:Dmel_CG10679.

Organism-specific databases

CTDi4738.
FlyBaseiFBgn0032725. Nedd8.

Phylogenomic databases

eggNOGiKOG0005. Eukaryota.
COG5272. LUCA.
InParanoidiQ9VJ33.
KOiK12158.
OMAiGKQMTDD.
OrthoDBiEOG091G178I.
PhylomeDBiQ9VJ33.

Miscellaneous databases

GenomeRNAii35151.
PROiQ9VJ33.

Gene expression databases

BgeeiFBgn0032725.
ExpressionAtlasiQ9VJ33. differential.
GenevisibleiQ9VJ33. DM.

Family and domain databases

InterProiIPR019956. Ubiquitin.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEDD8_DROME
AccessioniPrimary (citable) accession number: Q9VJ33
Secondary accession number(s): Q29QE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.