Q9VJ07 (PHLPP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase PHLPP-like protein
EC=3.1.3.16

Alternative name(s):
PH domain leucine-rich repeat protein phosphatase
dPHLPP

Gene names

Name:	Phlpp
ORF Names:	CG10493

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	954 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protein phosphatase that specifically mediates dephosphorylation of 'Ser-586' of Akt1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-586' of Akt1 triggers apoptosis and suppression of tumor growth. Ref.3
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Manganese By similarity.
Sequence similarities	Contains 14 LRR (leucine-rich) repeats. Contains 1 PP2C-like domain.

Ontologies

Keywords
Biological process	Apoptosis
Domain	Leucine-rich repeat Repeat
Ligand	Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW protein dephosphorylation Inferred from direct assay Ref.3. Source: UniProtKB regulation of apoptotic process Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 954	954	Protein phosphatase PHLPP-like protein		PRO_0000057787
Regions
	Repeat	69 – 89	21	LRR 1
	Repeat	90 – 104	15	LRR 2
	Repeat	110 – 131	22	LRR 3
	Repeat	135 – 156	22	LRR 4
	Repeat	158 – 179	22	LRR 5
	Repeat	183 – 202	20	LRR 6
	Repeat	206 – 227	22	LRR 7
	Repeat	231 – 252	22	LRR 8
	Repeat	255 – 275	21	LRR 9
	Repeat	279 – 300	22	LRR 10
	Repeat	303 – 324	22	LRR 11
	Repeat	326 – 347	22	LRR 12
	Repeat	348 – 369	22	LRR 13
	Repeat	372 – 393	22	LRR 14
	Domain	418 – 653	236	PP2C-like

Sequences

	Sequence		Length	Mass (Da)
	Q9VJ07 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7ADCF6F54F15957A	FASTA	954	107,092
	10 20 30 40 50 60 MLKATRKQAD PYKSKLKVSA SHSGPHPLPV EVTAAEEEQA ATFGQTSPQK LSLKGSQLGG 70 80 90 100 110 120 SILIGNYNYL TQLEVCENEM EVLDLSSLAQ LETLKCSRNK LMELIINGTN LQTLVADHNY 130 140 150 160 170 180 LHNISTTNTH PVPLKLQRID ISHNNFSELP NWVGACASLT AINASHNRLN NVAVLLRNYR 190 200 210 220 230 240 ITELVSLDLA YNDLKQLDQF PEGFSSIRSL QLQSNELPSL PDNFFAVTHA RLETLNVSCN 250 260 270 280 290 300 KLSTLPRYEQ NNHAALVNLS LAGNHLNDSI FEPLHNAAKL RVLHLAYNRI GVLPAACVRN 310 320 330 340 350 360 WPELEILVLS GNMLQQLPEE VATLGQLRVL RCCNNLLLCT PQLAKLAMLK VLDLSHNHLD 370 380 390 400 410 420 RVNLLALVPS RNLKYLDLSG NLQLQVDEQQ FKVCQSQSQR HWSLVDVSGN NRAALPTTKI 430 440 450 460 470 480 RQVSAQRNQN KTSGPWTMGF AETPGSGDCR KLSVYQLRAA NYGGSDEALY GMFEALEGRG 490 500 510 520 530 540 RAAQEMSHLV PDLMKQEQMV KDSAVRDYMK FTLLAAQQQC GSVRSAALFH LTRTRAPSKV 550 560 570 580 590 600 RPLKSKRYVL RMASTGGLDA YLIRRTSQLR LTKPDVIQKD QIHSMPDPHV LELILSNDDE 610 620 630 640 650 660 YLVVGNAQLW SVMDIDRAAR EIRKEENSLL AAKRLVDIAQ SFAAAESLSV IVVRFRHLGT 670 680 690 700 710 720 DVDHLIRELK QSVRKKPQPV SLPLSSGSVC KRTCCDRSNA CRHRAIEQEP LAGRSSPSGQ 730 740 750 760 770 780 SDRDLLAKDK DDEFVLAHAR VLQEEQQLEM LDETESVSES VLSEEQFKCW EYMLEQNTQL 790 800 810 820 830 840 LFDKELNTIS KSFTKQRTVP NAIMAATVLP ERNDFTSNLM RTVTNKFIST STPQLPQPIT 850 860 870 880 890 900 TSVPLGSYHQ VKQAPPGHFG SALSFQQAHS YGYNLFDAKP RPKFHGGTVK RSAGPNSAYF 910 920 930 940 950 GSLQRLMPYN FEYDFAVTQE RERNILDEEE HDDDDFNEHE SRMRKYWGVA TTEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." Gao T., Furnari F., Newton A.C. Mol. Cell 18:13-24(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAF53751.1.
RefSeq	NP_001260558.1. NM_001273629.1. NP_609938.1. NM_136094.2.
UniGene	Dm.17963.
3D structure databases
ProteinModelPortal	Q9VJ07.
SMR	Q9VJ07. Positions 49-423.
ModBase	Search...
Proteomic databases
PRIDE	Q9VJ07.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081149; FBpp0080693; FBgn0032749.
GeneID	35178.
KEGG	dme:Dmel_CG10493.
Organism-specific databases
CTD	35178.
FlyBase	FBgn0032749. Phlpp.
Phylogenomic databases
eggNOG	NOG240924.
GeneTree	ENSGT00440000037833.
InParanoid	Q9VJ07.
KO	K16340.
OMA	VCENEME.
OrthoDB	EOG4WH71N.
PhylomeDB	Q9VJ07.
Gene expression databases
Bgee	Q9VJ07.
GermOnline	CG10493. Drosophila melanogaster.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001611. Leu-rich_rpt. IPR001932. PP2C-like. [Graphical view]
Pfam	PF00560. LRR_1. 2 hits. [Graphical view]
SMART	SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS51450. LRR. 12 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	35178.
NextBio	792238.

Entry information

Entry name

PHLPP_DROME

Accession

Primary (citable) accession number: Q9VJ07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project