Q9VIS1 (CDGAP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase-activating protein CdGAPr

Alternative name(s):
d-CdGAPr

Gene names

Name:	CdGAPr
ORF Names:	CG10538

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1843 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably functions as a GTPase-activating protein (GAP) for RAC1 and/or CDC42. Required for optic stalk formation. Ref.6
Tissue specificity	Ubiquitously expressed. Ref.1
Developmental stage	Expressed at all developmental stages, with highest expression at the posterior pole of the early cellular blastoderm, the neuro-ectoderm prior to neuroblast delamination, rows of epidermal cells in the most posterior part of thoracic and first abdominal segments and a ring of epidermal cells at the posterior end of the embryo. Ref.1
Sequence similarities	Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
Sequence caution	The sequence AAM49910.1 differs from that shown. Reason: Erroneous initiation. The sequence AAT94493.1 differs from that shown. Reason: Frameshift at position 351.

Ontologies

Keywords
Domain	Coiled coil SH3 domain
Molecular function	Developmental protein GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	retinal ganglion cell axon guidance Inferred from mutant phenotype Ref.1Ref.6. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1843	1843	GTPase-activating protein CdGAPr		PRO_0000320117
Regions
	Domain	295 – 363	69	SH3
	Domain	424 – 618	195	Rho-GAP
	Coiled coil	1378 – 1404	27	Potential
	Compositional bias	632 – 642	11	Poly-Ala
	Compositional bias	957 – 960	4	Poly-Pro
	Compositional bias	1041 – 1045	5	Poly-Ser
	Compositional bias	1171 – 1174	4	Poly-Gln
	Compositional bias	1244 – 1248	5	Poly-Ala
Amino acid modifications
	Modified residue	166	1	Phosphoserine Ref.8
	Modified residue	168	1	Phosphoserine Ref.8
	Modified residue	180	1	Phosphoserine Ref.8
	Modified residue	837	1	Phosphoserine Ref.8
	Modified residue	840	1	Phosphoserine Ref.8
	Modified residue	843	1	Phosphothreonine Ref.8
	Modified residue	851	1	Phosphoserine Ref.8
	Modified residue	861	1	Phosphoserine Ref.8
	Modified residue	872	1	Phosphoserine Ref.8
	Modified residue	876	1	Phosphoserine Ref.8
	Modified residue	1029	1	Phosphoserine Ref.8
	Modified residue	1118	1	Phosphothreonine Ref.8
	Modified residue	1121	1	Phosphothreonine Ref.8
	Modified residue	1125	1	Phosphoserine Ref.8
	Modified residue	1216	1	Phosphoserine Ref.7
	Modified residue	1281	1	Phosphoserine Ref.8
	Modified residue	1284	1	Phosphoserine Ref.8
	Modified residue	1453	1	Phosphoserine Ref.8
	Modified residue	1456	1	Phosphoserine Ref.8
	Modified residue	1679	1	Phosphothreonine Ref.8
Experimental info
	Sequence conflict	1092	1	Missing in AAT94493. Ref.4
	Sequence conflict	1092	1	Missing in AAM49910. Ref.5
	Sequence conflict	1818 – 1820	3	NNR → K in AAM49910. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9VIS1 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: 4D5C65C637765D57	FASTA	1,843	204,217
	10 20 30 40 50 60 MSDKVSTSLA AQLHSHALAS AEAGGIGGVN GGPIACHAST DKDAQAPPLQ FEMDAPAAAI 70 80 90 100 110 120 SAQLNFKIVP ADSLPGLSYQ DMFASMNTSQ ISNASTESSC SPPIQGAVPP PKPSRHMAVQ 130 140 150 160 170 180 PLNSKSCRFP KLEECAHFHY ERVQLGPLSV QLLDDKSEHM GSSIASQSLG GDLPHSSLRS 190 200 210 220 230 240 FSPESCWFII RVCPQRCEPF LIKRSFENMQ LLDEMLHRCV YDRKISGLRN MEELAAELPS 250 260 270 280 290 300 ESDVEYAVAK YLERFSKIAS DSLTCGTILT WLQLDNKGRR LPLADGETQR TINTPAVGAA 310 320 330 340 350 360 YGVRRYQAQA PDEINIEVGD MISVIDMPSP AESIWWRGKK SHLQKSLYEV GFFPQSCVAT 370 380 390 400 410 420 IGDKVPRNFP MPAPLVGHLD ASPTKPVLRK HGKLIAFFRS FILSRPSRRR LKQSGIYRER 430 440 450 460 470 480 VFNCDLSEHL LNSGQDIPMV LRSCAEFIEN YGVIDGIYRL SGITSNIQRL RRAFDEERVP 490 500 510 520 530 540 DLGNPEMKQD IHAVSSLLKM YFRELPNPLC TYQLYDNFVE AIQVKADEAD ERLRLMKETV 550 560 570 580 590 600 LKLPPPHYRT LKYLAEHLYK VSQHHGRTGM TDKNLAIVWA PNLLRSPALE SGGVAALRGV 610 620 630 640 650 660 GVQAVVTEYL IRNCHNIFDA LDDHPARHSM VASATAAAAN AAGGELRLES LTDCESLLVE 670 680 690 700 710 720 QREQDQSLGV VERPKSLSTG GAKLISLEEA QERHSRVEGA DLKQSLPISM LTSASSNAAS 730 740 750 760 770 780 NIGSYIEVGG GPSSLPDKYH TVLSAPRSWQ KRKPDKTPSW KSIFSRSQRQ GNPDPGQKIT 790 800 810 820 830 840 TDVKDSVASR VSFVQASHAH ASKELTKHDK PKSIELLETT SNERDPKPMD LCIRSNSIDS 850 860 870 880 890 900 LRTVGHSRSV SHDSYFDLLQ SPQRGHMTTC PSRELSELGL NFDREEPEMR IFSESESLVS 910 920 930 940 950 960 SPRVGKENVP PASGSATRRI MRARPEDFSS QTNSVNPSPK KQPRLNLLSP SSARTMPPPP 970 980 990 1000 1010 1020 LSAPGTASSS CGHESGGAEN CCKRYKLEDQ LCDIQFIDCG TPENVPTTQQ QFASVEVHPP 1030 1040 1050 1060 1070 1080 PKPARANQSP ISSSNGASVG SSSSSTRYSY PSVQLGAKRK EQQDAKERFS YQGTFTQPGQ 1090 1100 1110 1120 1130 1140 KQESSAPRPV HSTNNGATLR KPRVEPVEDG QIKLHVPTPT TPARSPRYSL LLCDTESSDN 1150 1160 1170 1180 1190 1200 SSAVNTPQYD MEPLMLTSAM SGVSGVSSNV QQQQLLLGVD ASSSYLGSSH ESLGQNINNH 1210 1220 1230 1240 1250 1260 HDAEQRDMNA LKRELSLDLN PLQPRLPQPA NRAATLPVKD QLQAAAAAMC SSPNNSNFTD 1270 1280 1290 1300 1310 1320 NTSQSVTPSE YGYQHLQRQL SMHSLLATDE SSPVYEDFEQ TPVKMVPSPI KSTISITYKS 1330 1340 1350 1360 1370 1380 PEKEKKPSAI LETNFDENTV YEQVKLFRNS VTEVNQMLHE RSSLKQIAEE EQHDGGAYKA 1390 1400 1410 1420 1430 1440 AEMTQQLLQL EQEHHDHEEQ QEKEPEDEEQ SQLLYENIEL RKPKTVYENL RGEEMKFITE 1450 1460 1470 1480 1490 1500 EQKPNVDRIE LDSLDSLPDN MEHEQSSPSK SPTFSVKELA NKFESSPVEQ LPNFDFSVRG 1510 1520 1530 1540 1550 1560 GSMKKPNELS VPKTMPAPVP LKKLNSSAQK ITRSLDENAF VREFGGKQLQ DLSLSNKLPE 1570 1580 1590 1600 1610 1620 VMSEVNSRRK SFDFTRPKTL NPPKRLPGMS ITEEICQKRV GEMEAITPTT ENRISLIQQN 1630 1640 1650 1660 1670 1680 NMPKEQQSSA NQFLPPAGRK NVLTGVVLDR ERIDKIKEER RQQLTQKYYG DTLKSRSRTE 1690 1700 1710 1720 1730 1740 LNSEDNFPAA ESLRIKSKSR GDMHALQKDI DMNLKQLSRV TGGGSECTLH TGLSQGNGQE 1750 1760 1770 1780 1790 1800 HLGQQRVRRI SDEKNQNCDT SNGGVSGITS TSLLSVKTTA QKYGSTSKTP ANKFERSQPM 1810 1820 1830 1840 PRDRLQRNSL AESNAGTNNR EKISPQFSIR DVTAMFESRS QNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dynamic expression of d-CdGAPr, a novel Drosophila melanogaster gene encoding a GTPase activating protein." Sagnier T., Grienenberger A., Mariol M.-C., Berenger H., Pradel J., Graba Y. Mech. Dev. 94:267-270(2000) [PubMed: 10842085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1062-1843. Strain: Berkeley. Tissue: Embryo.
[6]	"Focal adhesion kinase controls morphogenesis of the Drosophila optic stalk." Murakami S., Umetsu D., Maeyama Y., Sato M., Yoshida S., Tabata T. Development 134:1539-1548(2007) [PubMed: 17360775] [Abstract] Cited for: FUNCTION.
[7]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1216, MASS SPECTROMETRY.
[8]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-166; SER-168; SER-180; SER-837; SER-840; THR-843; SER-851; SER-861; SER-872; SER-876; SER-1029; THR-1118; THR-1121; SER-1125; SER-1281; SER-1284; SER-1453; SER-1456 AND THR-1679, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF218776 mRNA. Translation: AAF44627.1. AE014134 Genomic DNA. Translation: AAF53844.2. BT015264 mRNA. Translation: AAT94493.1. Frameshift. AY118541 mRNA. Translation: AAM49910.1. Different initiation.
RefSeq	NP_610002.1. NM_136158.3.
UniGene	Dm.3427.
3D structure databases
HSSP	HSSP built from PDB template 1XA6 based on UniProtKB P52757.
ProteinModelPortal	Q9VIS1.
SMR	Q9VIS1. Positions 299-359, 420-620.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1558952.
STRING	Q9VIS1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081318; FBpp0080851; FBgn0032821.
GeneID	35267.
KEGG	dme:Dmel_CG10538.
NMPDR	fig|7227.3.peg.3052.
UCSC	CG10538-RA. d. melanogaster.
Organism-specific databases
CTD	35267.
FlyBase	FBgn0032821. CdGAPr.
Phylogenomic databases
eggNOG	inNOG07872.
GeneTree	EMGT00050000001404.
InParanoid	Q9VIS1.
OMA	AMFESRS.
OrthoDB	EOG4CNP5W.
PhylomeDB	Q9VIS1.
Gene expression databases
ArrayExpress	Q9VIS1.
Bgee	Q9VIS1.
Family and domain databases
InterPro	IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR011511. SH3_2. IPR001452. SH3_domain. [Graphical view]
Gene3D	G3DSA:1.10.555.10. RhoGAP. 1 hit.
Pfam	PF00620. RhoGAP. 1 hit. PF07653. SH3_2. 1 hit. [Graphical view]
SMART	SM00324. RhoGAP. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS50238. RHOGAP. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	792708.

Entry information

Entry name

CDGAP_DROME

Accession

Primary (citable) accession number: Q9VIS1
Secondary accession number(s): Q6AWI4, Q8MSV9, Q9NCL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project