Q9VIP7 (ACASE_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline ceramidase
Short name=AlkCDase
EC=3.5.1.23

Alternative name(s):
Alkaline N-acylsphingosine amidohydrolase
Alkaline acylsphingosine deacylase
Protein brainwashing

Gene names

Name:	bwa
ORF Names:	CG13969

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in the central midgut of late embryos. In brain, it is present at the interhemispheric junction and in groups of cells in the central brain. Ref.5 Ref.6
Disruption phenotype	Discrete defect in the ellipsoid body. Ref.5
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	brain development Inferred from mutant phenotype Ref.5. Source: FlyBase ceramide catabolic process Inferred from sequence or structural similarity. Source: UniProtKB regulation of lipid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ceramidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Alkaline ceramidase		PRO_0000247752
Regions
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	69 – 89	21	Helical; Potential
	Transmembrane	98 – 118	21	Helical; Potential
	Transmembrane	134 – 151	18	Helical; Potential
	Transmembrane	154 – 174	21	Helical; Potential
	Transmembrane	187 – 209	23	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
Experimental info
	Sequence conflict	19	1	H → Q in AAL55991. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9VIP7 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: C0DC37BCB69807A6	FASTA	283	32,793
	10 20 30 40 50 60 MGGMGGGGLL DIYAMAWEHL RPGSSPVDWC EGNYLISSNI AEFVNTFSNF LFILLPPVLI 70 80 90 100 110 120 MLFKEYGRFV TPGIHVIWVL LIVVGLSSMY FHATLSLIGQ LLDELAILWV FMAAFSLFYP 130 140 150 160 170 180 KRYYPKFVKN DRKTFSWLML LSAIAATGLS WWKPIVNAFV LMFMSVPTMV MLYTELQRVS 190 200 210 220 230 240 DQRVYRLGIR STTVWAVAVF CWINDRIFCE AWSSINFPYL HGFWHIFIFI AAYTVLVLFA 250 260 270 280 YFYVESELPQ RQPLLKYWPK NEFEFGIPFI SIRNPGKALR NTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pascual A., Boquet I., Preat T. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Central brain postembryonic development in Drosophila: implication of genes expressed at the interhemispheric junction." Boquet I., Hitier R., Dumas M., Chaminade M., Preat T. J. Neurobiol. 42:33-48(2000) [PubMed: 10623899] [Abstract] Cited for: DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
[6]	"Metabolism of sphingosine 1-phosphate and lysophosphatidic acid: a genome wide analysis of gene expression in Drosophila." Renault A.D., Starz-Gaiano M., Lehmann R. Mech. Dev. 119:S293-S301(2002) [PubMed: 14516700] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF323976 mRNA. Translation: AAL55991.1. AE014134 Genomic DNA. Translation: AAF53869.2. AY071232 mRNA. Translation: AAL48854.1.
RefSeq	NP_610020.1. NM_136176.2.
UniGene	Dm.14445.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9VIP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0081331; FBpp0080863; FBgn0045064.
GeneID	250736.
KEGG	dme:Dmel_CG13969.
Organism-specific databases
CTD	250736.
FlyBase	FBgn0045064. bwa.
Phylogenomic databases
eggNOG	inNOG06832.
GeneTree	EMGT00050000016840.
InParanoid	Q9VIP7.
OMA	KRCDNVR.
OrthoDB	EOG4T4BB9.
PhylomeDB	Q9VIP7.
Gene expression databases
ArrayExpress	Q9VIP7.
Bgee	Q9VIP7.
GermOnline	CG13969. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008901. Ceramidase. [Graphical view]
KO	K01441.
Pfam	PF05875. Ceramidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	843505.

Entry information

Entry name

ACASE_DROME

Accession

Primary (citable) accession number: Q9VIP7
Secondary accession number(s): Q8SYZ0, Q8WSF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project