Reviewed, UniProtKB/Swiss-Prot Q9VI75 (PICA_DROME)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-binding clathrin assembly protein LAP
Alternative name(s):
    Like-AP180

Gene names

Name:	lap
ORF Names:	CG2520

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Ref.1
Subunit structure	Binds clathrin and phosphatidylinositol-4,5-bisphosphate By similarity. UniProtKB Q13492 UniProtKB O55012
Subcellular location	Membrane › clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle. Note: Colocalized with clathrin in the Golgi area. Ref.1
Tissue specificity	In embryos, expression is seen in central and peripheral nervous systems (brain and ventral nerve cord) and Garland cells. Coexpressed with clathrin at presynaptic boutons of neuromuscular junctions. Ref.1
Sequence similarities	Contains 1 ENTH (epsin N-terminal homology) domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Coated pit Cytoplasmic vesicle Golgi apparatus Membrane
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	clathrin coat assembly Inferred from electronic annotation. Source: InterPro nerve-nerve synaptic transmission Inferred from mutant phenotype. Source: FlyBase neurotransmitter secretion Non-traceable author statement. Source: FlyBase phototaxis Inferred from mutant phenotype. Source: FlyBase receptor-mediated endocytosis Ref.1 Inferred from direct assay. Source: UniProtKB synaptic vesicle coating Traceable author statement. Source: FlyBase
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin coat Inferred from electronic annotation. Source: InterPro coated pit Ref.1 Inferred from direct assay. Source: UniProtKB synaptic vesicle Traceable author statement. Source: FlyBase
Molecular function	clathrin binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VIF6	1	EBI-175142,EBI-131604

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 468

468

Phosphatidylinositol-binding clathrin assembly protein LAP

PRO_0000187066

Regions

Domain

16 – 158

143

ENTH

Compositional bias

364 – 439

76

Ala-rich

Experimental info

Sequence conflict

51

1

T → A in AAD08669. Ref.1

Sequence conflict

209

1

M → I in AAM75091. Ref.4

Sequence conflict

372

1

T → A in AAD08669. Ref.1

Sequence conflict

372

1

T → A in AAM75091. Ref.4

Secondary structure

1

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468

Helix Strand Turn

Details...

Helix

24 – 31

8

Beta strand

34 – 37

4

Helix

41 – 52

12

Helix

58 – 69

12

Helix

74 – 90

17

Helix

93 – 101

9

Helix

129 – 154

26

Helix

158 – 160

3

Turn

170 – 172

3

Helix

175 – 193

19

Helix

199 – 201

3

Helix

205 – 236

32

Helix

242 – 270

29

Helix

275 – 277

3

Helix

286 – 297

12

Sequences

	Sequence		Length	Mass (Da)
	Q9VI75-1 [UniParc]. Last modified October 23, 2007. Version 3. Checksum: 4B3011615AF007A0	FASTA	468	49,867
	10 20 30 40 50 60 MTMAGQTIND RLLAARHSLA GQGLAKSVCK ATTEECIGPK KKHLDYLVHC TNEPNVSIPH 70 80 90 100 110 120 LANLLIERSQ NANWVVVYKS LITTHHLMAY GNERFMQYLA SSNSTFNLSS FLDKGTVQDG 130 140 150 160 170 180 GMGVPGGRMG YDMSPFIRRY AKYLNEKSLS YRAMAFDFCK VKRGKEEGSL RSMNAEKLLK 190 200 210 220 230 240 TLPVLQAQLD ALLEFDCQSN DLSNGVINMS FMLLFRDLIR LFACYNDGII NLLEKYFDMN 250 260 270 280 290 300 KKHARDALDL YKKFLVRMDR VGEFLKVAEN VGIDKGDIPD LTKAPSSLLD ALEQHLATLE 310 320 330 340 350 360 GRKVSAANTP TQSSSSAFGT AAASSKFDTT NGIDEQLKAQ VLAEEEAAMN QYKSKVSSPT 370 380 390 400 410 420 SSGAAGASAA LTNPFLSSPP AAQAGQPIVD LFGAASAQPA AAAAATKASD DLLQLGNPFA 430 440 450 460 DMFDASGGGA AAVGATGNAG DGTAKYDGGA GSSPFDWGAT DDDGGAAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Synaptic vesicle size and number are regulated by a clathrin adaptor protein required for endocytosis." Zhang B., Koh Y.H., Beckstead R.B., Budnik V., Ganetzky B., Bellen H.J. Neuron 21:1465-1475(1998) [PubMed: 9883738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Head.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[5]	"A novel all helix fold of the AP180 amino-terminal domain for phosphoinositide binding and clathrin assembly in synaptic vesicle endocytosis." Mao Y., Chen J., Maynard J.A., Zhang B., Quiocho F.A. Cell 104:433-440(2001) [PubMed: 11239400] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 4-301.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF075247 mRNA. Translation: AAD08669.1.
AE014297 Genomic DNA. Translation: AAF54050.1.
AY128498 mRNA. Translation: AAM75091.1.

RefSeq

NP_524252.2.

UniGene

Dm.2478

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HX8	X-ray	2.20	A/B	1-299	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9VI75. 4 interactions.

Proteomic databases

PRIDE

Q9VI75.

Genome annotation databases

Ensembl

FBgn0086372. Drosophila melanogaster. [Contig view]

GeneID

40863.

KEGG

dme:Dmel_CG2520.

NMPDR

fig|7227.3.peg.11518.

Organism-specific databases

FlyBase

FBgn0086372. lap.

Phylogenomic databases

HOGENOM

Q9VI75.

OMA

Q9VI75. AGQTIND.

Gene expression databases

ArrayExpress

Q9VI75.

GermOnline

CG2520. Drosophila melanogaster.

Family and domain databases

InterPro

IPR011417. ANTH.
IPR014712. Clathrin_Pinositid-bd_GAT-like.
IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
[Graphical view]

Gene3D

G3DSA:1.25.40.90. ENTH_VHS. 1 hit.
G3DSA:1.20.58.150. Pinositid-bd_clathrin_GAT-like. 1 hit.

Pfam

PF07651. ANTH. 1 hit.
[Graphical view]

SMART

SM00273. ENTH. 1 hit.
[Graphical view]

PROSITE

PS50942. ENTH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

821020.

Entry information

Entry name

PICA_DROME

Accession

Primary (citable) accession number: Q9VI75
Secondary accession number(s): O96528, Q8MQM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	October 23, 2007
Last modified:	June 16, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project