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Protein

ADP-ribosylation factor-like protein 8

Gene

Gie

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in lysosome motility (PubMed:16537643). Essential role in chromosome segregation (PubMed:15331635).2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 357GTPBy similarity
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi130 – 1334GTPBy similarity

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB
  • GDP binding Source: UniProtKB
  • GTP binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • response to hypoxia Source: FlyBase
  • sister chromatid segregation Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor-like protein 8
Alternative name(s):
Novel small G protein indispensable for equal chromosome segregation
Short name:
dGie
Gene namesi
Name:Gie
Synonyms:Arl8
ORF Names:CG7891
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037551. Gie.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei1 – 1919Note=Mediates targeting to membranesBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extrinsic component of lysosome membrane Source: UniProtKB
  • lysosome Source: FlyBase
  • midbody Source: UniProtKB
  • spindle midzone Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Chromosome mis-segregation occurs but mitotic progression continues.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 186186ADP-ribosylation factor-like protein 8PRO_0000232927Add
BLAST

Proteomic databases

PaxDbiQ9VHV5.
PRIDEiQ9VHV5.

Expressioni

Gene expression databases

BgeeiQ9VHV5.
GenevisibleiQ9VHV5. DM.

Interactioni

Subunit structurei

Interacts with tubulin.1 Publication

GO - Molecular functioni

  • alpha-tubulin binding Source: UniProtKB
  • beta-tubulin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi66144. 32 interactions.
MINTiMINT-967237.
STRINGi7227.FBpp0081290.

Structurei

3D structure databases

ProteinModelPortaliQ9VHV5.
SMRiQ9VHV5. Positions 18-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
InParanoidiQ9VHV5.
KOiK07955.
OMAiHREVCCY.
OrthoDBiEOG7F24V2.
PhylomeDBiQ9VHV5.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VHV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALINRILE WFKSIFWKEE MELTLVGLQF SGKTTFVNVI ASGQFAEDMI
60 70 80 90 100
PTVGFNMRKI TRGNVTIKVW DIGGQPRFRS MWERYCRGVN AIVYMVDAAD
110 120 130 140 150
LDKLEASRNE LHSLLDKPQL AGIPVLVLGN KRDLPGALDE TGLIERMNLS
160 170 180
SIQDREICCY SISCKEKDNI DITLQWLIQH SKSQSR
Length:186
Mass (Da):21,254
Last modified:May 1, 2000 - v1
Checksum:iB437D8EC4D001EE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB185208 mRNA. Translation: BAD30092.1.
AE014297 Genomic DNA. Translation: AAF54194.1.
AY094809 mRNA. Translation: AAM11162.1.
RefSeqiNP_001287225.1. NM_001300296.1.
NP_001287226.1. NM_001300297.1.
NP_649769.1. NM_141512.3.
UniGeneiDm.11405.

Genome annotation databases

EnsemblMetazoaiFBtr0081794; FBpp0081290; FBgn0037551.
FBtr0339723; FBpp0308779; FBgn0037551.
FBtr0339724; FBpp0308780; FBgn0037551.
GeneIDi40961.
KEGGidme:Dmel_CG7891.
UCSCiCG7891-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB185208 mRNA. Translation: BAD30092.1.
AE014297 Genomic DNA. Translation: AAF54194.1.
AY094809 mRNA. Translation: AAM11162.1.
RefSeqiNP_001287225.1. NM_001300296.1.
NP_001287226.1. NM_001300297.1.
NP_649769.1. NM_141512.3.
UniGeneiDm.11405.

3D structure databases

ProteinModelPortaliQ9VHV5.
SMRiQ9VHV5. Positions 18-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66144. 32 interactions.
MINTiMINT-967237.
STRINGi7227.FBpp0081290.

Proteomic databases

PaxDbiQ9VHV5.
PRIDEiQ9VHV5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0081794; FBpp0081290; FBgn0037551.
FBtr0339723; FBpp0308779; FBgn0037551.
FBtr0339724; FBpp0308780; FBgn0037551.
GeneIDi40961.
KEGGidme:Dmel_CG7891.
UCSCiCG7891-RA. d. melanogaster.

Organism-specific databases

CTDi40961.
FlyBaseiFBgn0037551. Gie.

Phylogenomic databases

eggNOGiKOG0075. Eukaryota.
ENOG410XNUK. LUCA.
GeneTreeiENSGT00830000128269.
InParanoidiQ9VHV5.
KOiK07955.
OMAiHREVCCY.
OrthoDBiEOG7F24V2.
PhylomeDBiQ9VHV5.

Miscellaneous databases

ChiTaRSiGie. fly.
GenomeRNAii40961.
PROiQ9VHV5.

Gene expression databases

BgeeiQ9VHV5.
GenevisibleiQ9VHV5. DM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation."
    Okai T., Araki Y., Tada M., Tateno T., Kontani K., Katada T.
    J. Cell Sci. 117:4705-4715(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH TUBULIN, DISRUPTION PHENOTYPE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility."
    Hofmann I., Munro S.
    J. Cell Sci. 119:1494-1503(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiARL8_DROME
AccessioniPrimary (citable) accession number: Q9VHV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.