Reviewed, UniProtKB/Swiss-Prot Q9VHS4 (OR85A_DROME)

Names and origin

Protein names

Recommended name:
    Putative odorant receptor 85a

Gene names

Name:	Or85a
ORF Names:	CG7454

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Or83b. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yellow-b	Q9VJI5	1	EBI-183787,EBI-196229

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Putative odorant receptor 85a		PRO_0000174274
Regions
	Topological domain	1 – 46	46	Cytoplasmic Potential
	Transmembrane	47 – 67	21	1 Potential
	Topological domain	68 – 83	16	Extracellular Potential
	Transmembrane	84 – 104	21	2 Potential
	Topological domain	105 – 142	38	Cytoplasmic Potential
	Transmembrane	143 – 163	21	3 Potential
	Topological domain	164 – 192	29	Extracellular Potential
	Transmembrane	193 – 213	21	4 Potential
	Topological domain	214 – 262	49	Cytoplasmic Potential
	Transmembrane	263 – 283	21	5 Potential
	Topological domain	284 – 294	11	Extracellular Potential
	Transmembrane	295 – 315	21	6 Potential
	Topological domain	316 – 347	32	Cytoplasmic Potential
	Transmembrane	348 – 368	21	7 Potential
	Topological domain	369 – 397	29	Extracellular Potential
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VHS4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 4ED07645226497C2	FASTA	397	45,820
	10 20 30 40 50 60 MIFKYIQEPV LGSLFRSRDS LIYLNRSIDQ MGWRLPPRTK PYWWLYYIWT LVVIVLVFIF 70 80 90 100 110 120 IPYGLIMTGI KEFKNFTTTD LFTYVQVPVN TNASIMKGII VLFMRRRFSR AQKMMDAMDI 130 140 150 160 170 180 RCTKMEEKVQ VHRAAALCNR VVVIYHCIYF GYLSMALTGA LVIGKTPFCL YNPLVNPDDH 190 200 210 220 230 240 FYLATAIESV TMAGIILANL ILDVYPIIYV VVLRIHMELL SERIKTLRTD VEKGDDQHYA 250 260 270 280 290 300 ELVECVKDHK LIVEYGNTLR PMISATMFIQ LLSVGLLLGL AAVSMQFYNT VMERVVSGVY 310 320 330 340 350 360 TIAILSQTFP FCYVCEQLSS DCESLTNTLF HSKWIGAERR YRTTMLYFIH NVQQSILFTA 370 380 390 GGIFPICLNT NIKMAKFAFS VVTIVNEMDL AEKLRRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAF54225.1.
RefSeq	NP_524277.1.
UniGene	Dm.27235
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22791N.
IntAct	Q9VHS4. 1 interaction.
Proteomic databases
PRIDE	Q9VHS4.
Genome annotation databases
Ensembl	FBgn0037576. Drosophila melanogaster. [Contig view]
GeneID	40991.
KEGG	dme:Dmel_CG7454.
NMPDR	fig|7227.3.peg.11681.
Organism-specific databases
FlyBase	FBgn0037576. Or85a.
Phylogenomic databases
HOGENOM	Q9VHS4.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-010531-MON.
Gene expression databases
ArrayExpress	Q9VHS4.
GermOnline	CG7454. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	821630.

Entry information

Entry name

OR85A_DROME

Accession

Primary (citable) accession number: Q9VHS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project