Reviewed, UniProtKB/Swiss-Prot Q9VHE6 (OR85F_DROME)

Names and origin

Protein names

Recommended name:
    Putative odorant receptor 85f

Gene names

Name:	Or85f
ORF Names:	CG16755

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Or83b. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG42257	Q8MLU3	1	EBI-112877,EBI-127540

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Putative odorant receptor 85f		PRO_0000174279
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	1 Potential
	Topological domain	58 – 69	12	Extracellular Potential
	Transmembrane	70 – 90	21	2 Potential
	Topological domain	91 – 130	40	Cytoplasmic Potential
	Transmembrane	131 – 151	21	3 Potential
	Topological domain	152 – 179	28	Extracellular Potential
	Transmembrane	180 – 200	21	4 Potential
	Topological domain	201 – 268	68	Cytoplasmic Potential
	Transmembrane	269 – 289	21	5 Potential
	Topological domain	290 – 295	6	Extracellular Potential
	Transmembrane	296 – 316	21	6 Potential
	Topological domain	317 – 363	47	Cytoplasmic Potential
	Transmembrane	364 – 384	21	7 Potential
	Topological domain	385 – 392	8	Extracellular Potential
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VHE6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 393C7E2811D3E404	FASTA	392	45,343
	10 20 30 40 50 60 MEPVQYSYED FARLPTTVFW IMGYDMLGVP KTRSRRILYW IYRFLCLASH GVCVGVMVFR 70 80 90 100 110 120 MVEAKTIDNV SLIMRYATLV TYIINSDTKF ATVLQRSAIQ SLNSKLAELY PKTTLDRIYH 130 140 150 160 170 180 RVNDHYWTKS FVYLVIIYIG SSIMVVIGPI ITSIIAYFTH NVFTYMHCYP YFLYDPEKDP 190 200 210 220 230 240 VWIYISIYAL EWLHSTQMVI SNIGADIWLL YFQVQINLHF RGIIRSLADH KPSVKHDQED 250 260 270 280 290 300 RKFIAKIVDK QVHLVSLQND LNGIFGKSLL LSLLTTAAVI CTVAVYTLIQ GPTLEGFTYV 310 320 330 340 350 360 IFIGTSVMQV YLVCYYGQQV LDLSGEVAHA VYNHDFHDAS IAYKRYLLII IIRAQQPVEL 370 380 390 NAMGYLSISL DTFKQLMSVS YRVITMLMQM IQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAF54368.1.
RefSeq	NP_524289.1.
UniGene	Dm.27246
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22461N.
IntAct	Q9VHE6. 1 interaction.
Proteomic databases
PRIDE	Q9VHE6.
Genome annotation databases
Ensembl	FBgn0037685. Drosophila melanogaster. [Contig view]
GeneID	41119.
KEGG	dme:Dmel_CG16755.
NMPDR	fig|7227.3.peg.11863.
Organism-specific databases
FlyBase	FBgn0037685. Or85f.
Phylogenomic databases
HOGENOM	Q9VHE6.
OMA	Q9VHE6. YIGSSIM.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-010710-MON.
Gene expression databases
ArrayExpress	Q9VHE6.
GermOnline	CG16755. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	822281.

Entry information

Entry name

OR85F_DROME

Accession

Primary (citable) accession number: Q9VHE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project