Q9VHE6 (OR85F_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative odorant receptor 85f

Gene names

Name:	Or85f
ORF Names:	CG16755

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the heteromeric odorant receptor channel (TC 1.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Snp	Q8MLU3	1	EBI-112877,EBI-127540

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Putative odorant receptor 85f		PRO_0000174279
Regions
	Topological domain	1 – 36	36	Cytoplasmic Potential
	Transmembrane	37 – 57	21	Helical; Name=1; Potential
	Topological domain	58 – 69	12	Extracellular Potential
	Transmembrane	70 – 90	21	Helical; Name=2; Potential
	Topological domain	91 – 130	40	Cytoplasmic Potential
	Transmembrane	131 – 151	21	Helical; Name=3; Potential
	Topological domain	152 – 179	28	Extracellular Potential
	Transmembrane	180 – 200	21	Helical; Name=4; Potential
	Topological domain	201 – 268	68	Cytoplasmic Potential
	Transmembrane	269 – 289	21	Helical; Name=5; Potential
	Topological domain	290 – 295	6	Extracellular Potential
	Transmembrane	296 – 316	21	Helical; Name=6; Potential
	Topological domain	317 – 363	47	Cytoplasmic Potential
	Transmembrane	364 – 384	21	Helical; Name=7; Potential
	Topological domain	385 – 392	8	Extracellular Potential
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9VHE6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 393C7E2811D3E404	FASTA	392	45,343
	10 20 30 40 50 60 MEPVQYSYED FARLPTTVFW IMGYDMLGVP KTRSRRILYW IYRFLCLASH GVCVGVMVFR 70 80 90 100 110 120 MVEAKTIDNV SLIMRYATLV TYIINSDTKF ATVLQRSAIQ SLNSKLAELY PKTTLDRIYH 130 140 150 160 170 180 RVNDHYWTKS FVYLVIIYIG SSIMVVIGPI ITSIIAYFTH NVFTYMHCYP YFLYDPEKDP 190 200 210 220 230 240 VWIYISIYAL EWLHSTQMVI SNIGADIWLL YFQVQINLHF RGIIRSLADH KPSVKHDQED 250 260 270 280 290 300 RKFIAKIVDK QVHLVSLQND LNGIFGKSLL LSLLTTAAVI CTVAVYTLIQ GPTLEGFTYV 310 320 330 340 350 360 IFIGTSVMQV YLVCYYGQQV LDLSGEVAHA VYNHDFHDAS IAYKRYLLII IIRAQQPVEL 370 380 390 NAMGYLSISL DTFKQLMSVS YRVITMLMQM IQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAF54368.1.
RefSeq	NP_524289.1. NM_079565.3.
UniGene	Dm.27246.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-22461N.
IntAct	Q9VHE6. 1 interaction.
MINT	MINT-863427.
STRING	Q9VHE6.
Proteomic databases
PRIDE	Q9VHE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0082028; FBpp0081506; FBgn0037685.
GeneID	41119.
KEGG	dme:Dmel_CG16755.
NMPDR	fig|7227.3.peg.11863.
Organism-specific databases
CTD	41119.
FlyBase	FBgn0037685. Or85f.
Phylogenomic databases
eggNOG	inNOG10124.
GeneTree	EMGT00050000009320.
InParanoid	Q9VHE6.
OMA	VQICMHF.
OrthoDB	EOG40ZPDD.
PhylomeDB	Q9VHE6.
Gene expression databases
GermOnline	CG16755. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
KO	K08471.
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	822281.

Entry information

Entry name

OR85F_DROME

Accession

Primary (citable) accession number: Q9VHE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project