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Protein

Probable maleylacetoacetate isomerase 2

Gene

GstZ2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid in vitro. Has no glutathione thioltransferase activity with 4-hydroxynonenal (4-HNE), adrenochrome, phenethyl isothiocyanate (PEITC), 5-hydroperoxyeicosatetraenoic acid ((5S)-HpETE), prostaglandin A2 (PGA2) or 2-hydroxyethyldisulfide (HED).1 Publication

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.1 Publication
RX + glutathione = HX + R-S-glutathione.1 Publication

Cofactori

glutathione1 PublicationNote: Glutathione is required for the MAAI activity.1 Publication

Pathwayi: L-phenylalanine degradation

This protein is involved in step 5 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Protein henna (Hn)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Homogentisate 1,2-dioxygenase (hgo)
  5. Probable maleylacetoacetate isomerase 1 (GstZ1), Probable maleylacetoacetate isomerase 2 (GstZ2)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei55GlutathioneBy similarity1
Binding sitei69Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei121GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione transferase activity Source: FlyBase
  • maleylacetoacetate isomerase activity Source: UniProtKB-EC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiR-DME-156590. Glutathione conjugation.
R-DME-71182. Phenylalanine and tyrosine catabolism.
UniPathwayiUPA00139; UER00340.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable maleylacetoacetate isomerase 2 (EC:5.2.1.2)
Short name:
MAAI 2
Alternative name(s):
Glutathione S-transferase zeta 2 (EC:2.5.1.18)
Gene namesi
Name:GstZ2
ORF Names:CG9363
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037697. GstZ2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860271 – 227Probable maleylacetoacetate isomerase 2Add BLAST227

Proteomic databases

PaxDbiQ9VHD2.
PRIDEiQ9VHD2.

Expressioni

Developmental stagei

Expressed during embryogenesis.1 Publication

Gene expression databases

BgeeiFBgn0037697.
GenevisibleiQ9VHD2. DM.

Interactioni

Protein-protein interaction databases

BioGridi66294. 1 interactor.
DIPiDIP-24005N.
IntActiQ9VHD2. 1 interactor.
MINTiMINT-1563429.
STRINGi7227.FBpp0081520.

Structurei

3D structure databases

ProteinModelPortaliQ9VHD2.
SMRiQ9VHD2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 97GST N-terminalAdd BLAST84
Domaini102 – 222GST C-terminalAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 29Glutathione bindingBy similarity6
Regioni81 – 82Glutathione bindingBy similarity2
Regioni125 – 127Glutathione bindingBy similarity3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00390000006580.
InParanoidiQ9VHD2.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG091G0QFQ.
PhylomeDBiQ9VHD2.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9VHD2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTNLCPNAS SSDIQPILYS YWRSSCSWRV RIAMNLKEIP YDIKPISLIK
60 70 80 90 100
SGGEQHCNEY REVNPMEQVP ALQIDGHTLI ESVAIMHYLE ETRPQRPLLP
110 120 130 140 150
QDVHKRAKVR EIVEIICSGI QPLQNLIVLI HVGEEKKKEW AQHWITRGFR
160 170 180 190 200
AVEKALSTSA GKYCVGDEIS MADCCLVPQV FNARRFHVDL RPYPIILRID
210 220
RELESNPAFR AAHPSNQPDC PPELPNK
Length:227
Mass (Da):25,975
Last modified:May 1, 2000 - v1
Checksum:iC708DD764F48C754
GO
Isoform B (identifier: Q9VHD2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSTNLCPNASSSDIQ → MSLSAIAK

Show »
Length:220
Mass (Da):25,227
Checksum:iDABCE0A9315EA989
GO
Isoform C (identifier: Q9VHD2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSTNLCPNASSSDIQ → MNH

Show »
Length:215
Mass (Da):24,808
Checksum:i86D4F06DCD77B834
GO

Sequence cautioni

The sequence AAL28280 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEV23904 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0102911 – 15MSTNL…SSDIQ → MSLSAIAK in isoform B. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0102921 – 15MSTNL…SSDIQ → MNH in isoform C. 1 PublicationAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54382.1.
AE014297 Genomic DNA. Translation: AAN13429.1.
AE014297 Genomic DNA. Translation: AAS65133.1.
AY060732 mRNA. Translation: AAL28280.2. Different initiation.
BT132880 mRNA. Translation: AEV23904.1. Different initiation.
BT133289 mRNA. Translation: AFC88878.1.
RefSeqiNP_649895.1. NM_141638.3. [Q9VHD2-1]
NP_731358.1. NM_169286.2. [Q9VHD2-2]
NP_996190.1. NM_206468.2. [Q9VHD2-3]
UniGeneiDm.1121.

Genome annotation databases

EnsemblMetazoaiFBtr0082042; FBpp0081520; FBgn0037697. [Q9VHD2-1]
GeneIDi41133.
KEGGidme:Dmel_CG9363.
UCSCiCG9363-RA. d. melanogaster. [Q9VHD2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54382.1.
AE014297 Genomic DNA. Translation: AAN13429.1.
AE014297 Genomic DNA. Translation: AAS65133.1.
AY060732 mRNA. Translation: AAL28280.2. Different initiation.
BT132880 mRNA. Translation: AEV23904.1. Different initiation.
BT133289 mRNA. Translation: AFC88878.1.
RefSeqiNP_649895.1. NM_141638.3. [Q9VHD2-1]
NP_731358.1. NM_169286.2. [Q9VHD2-2]
NP_996190.1. NM_206468.2. [Q9VHD2-3]
UniGeneiDm.1121.

3D structure databases

ProteinModelPortaliQ9VHD2.
SMRiQ9VHD2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66294. 1 interactor.
DIPiDIP-24005N.
IntActiQ9VHD2. 1 interactor.
MINTiMINT-1563429.
STRINGi7227.FBpp0081520.

Proteomic databases

PaxDbiQ9VHD2.
PRIDEiQ9VHD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082042; FBpp0081520; FBgn0037697. [Q9VHD2-1]
GeneIDi41133.
KEGGidme:Dmel_CG9363.
UCSCiCG9363-RA. d. melanogaster. [Q9VHD2-1]

Organism-specific databases

CTDi41133.
FlyBaseiFBgn0037697. GstZ2.

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00390000006580.
InParanoidiQ9VHD2.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG091G0QFQ.
PhylomeDBiQ9VHD2.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00340.
ReactomeiR-DME-156590. Glutathione conjugation.
R-DME-71182. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

GenomeRNAii41133.
PROiQ9VHD2.

Gene expression databases

BgeeiFBgn0037697.
GenevisibleiQ9VHD2. DM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAAI2_DROME
AccessioniPrimary (citable) accession number: Q9VHD2
Secondary accession number(s): H0RNF8, H8F4P9, Q8INN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.