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Q9VHD2

- MAAI2_DROME

UniProt

Q9VHD2 - MAAI2_DROME

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Protein

Probable maleylacetoacetate isomerase 2

Gene
GstZ2, CG9363
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid in vitro. Has no glutathione thioltransferase activity with 4-hydroxynonenal (4-HNE), adrenochrome, phenethyl isothiocyanate (PEITC), 5-hydroperoxyeicosatetraenoic acid ((5S)-HpETE), prostaglandin A2 (PGA2) or 2-hydroxyethyldisulfide (HED).1 Publication

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.1 Publication
RX + glutathione = HX + R-S-glutathione.1 Publication

Cofactori

Glutathione. Required for the MAAI activity.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei55 – 551Glutathione By similarity
Binding sitei69 – 691Glutathione; via amide nitrogen and carbonyl oxygen By similarity
Binding sitei121 – 1211Glutathione By similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: FlyBase
  2. maleylacetoacetate isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutathione metabolic process Source: FlyBase
  2. L-phenylalanine catabolic process Source: UniProtKB-UniPathway
  3. tyrosine catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiREACT_203229. Phenylalanine and tyrosine catabolism.
UniPathwayiUPA00139; UER00340.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable maleylacetoacetate isomerase 2 (EC:5.2.1.2)
Short name:
MAAI 2
Alternative name(s):
Glutathione S-transferase zeta 2 (EC:2.5.1.18)
Gene namesi
Name:GstZ2
ORF Names:CG9363
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037697. GstZ2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Probable maleylacetoacetate isomerase 2PRO_0000186027Add
BLAST

Proteomic databases

PaxDbiQ9VHD2.
PRIDEiQ9VHD2.

Expressioni

Developmental stagei

Expressed during embryogenesis.1 Publication

Gene expression databases

BgeeiQ9VHD2.

Interactioni

Protein-protein interaction databases

BioGridi66294. 1 interaction.
DIPiDIP-24005N.
IntActiQ9VHD2. 1 interaction.
MINTiMINT-1563429.

Structurei

3D structure databases

ProteinModelPortaliQ9VHD2.
SMRiQ9VHD2. Positions 15-224.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 9784GST N-terminalAdd
BLAST
Domaini102 – 222121GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 296Glutathione binding By similarity
Regioni81 – 822Glutathione binding By similarity
Regioni125 – 1273Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00390000006580.
InParanoidiQ9VHD2.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG7TF79P.
PhylomeDBiQ9VHD2.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q9VHD2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSTNLCPNAS SSDIQPILYS YWRSSCSWRV RIAMNLKEIP YDIKPISLIK    50
SGGEQHCNEY REVNPMEQVP ALQIDGHTLI ESVAIMHYLE ETRPQRPLLP 100
QDVHKRAKVR EIVEIICSGI QPLQNLIVLI HVGEEKKKEW AQHWITRGFR 150
AVEKALSTSA GKYCVGDEIS MADCCLVPQV FNARRFHVDL RPYPIILRID 200
RELESNPAFR AAHPSNQPDC PPELPNK 227
Length:227
Mass (Da):25,975
Last modified:May 1, 2000 - v1
Checksum:iC708DD764F48C754
GO
Isoform B (identifier: Q9VHD2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSTNLCPNASSSDIQ → MSLSAIAK

Show »
Length:220
Mass (Da):25,227
Checksum:iDABCE0A9315EA989
GO
Isoform C (identifier: Q9VHD2-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: MSTNLCPNASSSDIQ → MNH

Show »
Length:215
Mass (Da):24,808
Checksum:i86D4F06DCD77B834
GO

Sequence cautioni

The sequence AAL28280.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AEV23904.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1515MSTNL…SSDIQ → MSLSAIAK in isoform B. VSP_010291Add
BLAST
Alternative sequencei1 – 1515MSTNL…SSDIQ → MNH in isoform C. VSP_010292Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF54382.1.
AE014297 Genomic DNA. Translation: AAN13429.1.
AE014297 Genomic DNA. Translation: AAS65133.1.
AY060732 mRNA. Translation: AAL28280.2. Different initiation.
BT132880 mRNA. Translation: AEV23904.1. Different initiation.
BT133289 mRNA. Translation: AFC88878.1.
RefSeqiNP_649895.1. NM_141638.3. [Q9VHD2-1]
NP_731358.1. NM_169286.2. [Q9VHD2-2]
NP_996190.1. NM_206468.2. [Q9VHD2-3]
UniGeneiDm.1121.

Genome annotation databases

EnsemblMetazoaiFBtr0082042; FBpp0081520; FBgn0037697. [Q9VHD2-1]
GeneIDi41133.
KEGGidme:Dmel_CG9363.
UCSCiCG9363-RA. d. melanogaster. [Q9VHD2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF54382.1 .
AE014297 Genomic DNA. Translation: AAN13429.1 .
AE014297 Genomic DNA. Translation: AAS65133.1 .
AY060732 mRNA. Translation: AAL28280.2 . Different initiation.
BT132880 mRNA. Translation: AEV23904.1 . Different initiation.
BT133289 mRNA. Translation: AFC88878.1 .
RefSeqi NP_649895.1. NM_141638.3. [Q9VHD2-1 ]
NP_731358.1. NM_169286.2. [Q9VHD2-2 ]
NP_996190.1. NM_206468.2. [Q9VHD2-3 ]
UniGenei Dm.1121.

3D structure databases

ProteinModelPortali Q9VHD2.
SMRi Q9VHD2. Positions 15-224.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66294. 1 interaction.
DIPi DIP-24005N.
IntActi Q9VHD2. 1 interaction.
MINTi MINT-1563429.

Proteomic databases

PaxDbi Q9VHD2.
PRIDEi Q9VHD2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0082042 ; FBpp0081520 ; FBgn0037697 . [Q9VHD2-1 ]
GeneIDi 41133.
KEGGi dme:Dmel_CG9363.
UCSCi CG9363-RA. d. melanogaster. [Q9VHD2-1 ]

Organism-specific databases

CTDi 41133.
FlyBasei FBgn0037697. GstZ2.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00390000006580.
InParanoidi Q9VHD2.
KOi K01800.
OMAi RAQVRMI.
OrthoDBi EOG7TF79P.
PhylomeDBi Q9VHD2.

Enzyme and pathway databases

UniPathwayi UPA00139 ; UER00340 .
Reactomei REACT_203229. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

GenomeRNAii 41133.
NextBioi 822335.

Gene expression databases

Bgeei Q9VHD2.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR01262. maiA. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A preliminary characterization of the cytosolic glutathione transferase proteome from Drosophila melanogaster."
    Saisawang C., Wongsantichon J., Ketterman A.J.
    Biochem. J. 442:181-190(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, COFACTOR, DEVELOPMENTAL STAGE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Ovary.
  5. Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.
    Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS B AND C).

Entry informationi

Entry nameiMAAI2_DROME
AccessioniPrimary (citable) accession number: Q9VHD2
Secondary accession number(s): H0RNF8, H8F4P9, Q8INN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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