Q9VH90 (TRBID_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase trabid
Short name=dTrbd
EC=3.4.19.12

Gene names

Name:	trbd
ORF Names:	CG9448

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of the Wnt signaling pathway. Specifically cleaves 'Lys-63'-linked ubiquitin chains. May act by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription By similarity. Required for an efficient wg response, but not for other signaling responses, in the eye. Ref.4
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with Apc. Ref.4
Domain	The OTU domain mediates the deubiquitinating activity By similarity. The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin By similarity.
Disruption phenotype	Flies are viable and fertile, suggesting functional redundancy. Ref.4
Sequence similarities	Belongs to the peptidase C64 family. Contains 1 OTU domain. Contains 3 RanBP2-type zinc fingers.
Sequence caution	The sequence AAL39403.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Wnt signaling pathway
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of Wnt receptor signaling pathway Inferred from mutant phenotype Ref.4. Source: UniProtKB protein K63-linked deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.4. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 778	778	Ubiquitin thioesterase trabid		PRO_0000361559
Regions
	Domain	507 – 665	159	OTU
	Zinc finger	5 – 36	32	RanBP2-type 1
	Zinc finger	89 – 118	30	RanBP2-type 2
	Zinc finger	232 – 261	30	RanBP2-type 3
	Compositional bias	191 – 230	40	Gln-rich
Sites
	Active site	518	1	Nucleophile By similarity
	Active site	658	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	770	1	Phosphoserine Ref.5
	Modified residue	771	1	Phosphoserine Ref.5
	Modified residue	775	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9VH90 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 57AAF0A860032F07	FASTA	778	88,199
	10 20 30 40 50 60 MCDTKDDAQK WKCETCTYEN YPSSLKCTMC QASKPLLNED IFRLSPAQES CTVAEEAAAV 70 80 90 100 110 120 EVAVMSPTPS STCYSLQPQS QARQSNVADS EKWPCKVCTY LNWPRSLRCV QCCTKRGGEA 130 140 150 160 170 180 IERGKKDMDN EADGDRAGEA LQALRISGSE ENLANKPVQL IGATASHRLS LSRGIDDATH 190 200 210 220 230 240 LNNLANASHN QSQSQHRQPV LQQQMQLQLQ PQQQRESSSS AAVPPQQQKQ CYVSKWACNS 250 260 270 280 290 300 CTYENWPRSI KCSMCGKTRE REISGSQNDL HASSSLNSQE ENQQQLQQPN VDTVSVNNSF 310 320 330 340 350 360 NKKHIYQLGS SETINNCDTL QERQERRQRQ IRRQVDWQWL NACLGVVENN YSAVEAYLSC 370 380 390 400 410 420 GGNPARSLTS TEIAALNRNS AFDVGHTLIH LAIRFHREEM LPMLLDQISG SGPGIKRVPS 430 440 450 460 470 480 YVAPDLAADI RRHFANTLRL RKSGLPCHYV QKHATFALPA EIEELPIPIQ EQLYDELLDR 490 500 510 520 530 540 DAQKQLETPP PALNWSLEIT ARLSSRMFVL WNRSAGDCLL DSAMQATWGV FDRDNILRRA 550 560 570 580 590 600 LADTLHQCGH VFFTRWKEYE MLQASMLHFT LEDSQFEEDW STLLSLAGQP GSSLEQLHIF 610 620 630 640 650 660 ALAHILRRPI IVYGVKYVKS FRGEDIGYAR FEGVYLPLFW DQNFCTKSPI ALGYTRGHFS 670 680 690 700 710 720 ALVPMEPFTR IDGRRDDVED VTYLPLMDCE LKLLPIHFLT QSEVGNEESM MRQWLDVCVT 730 740 750 760 770 DGGLLVAQQK LSKRPLLVAQ MLEEWLNHYR RIAQVITAPF IRRPQITHYS SDGDSDEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[4]	"Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains." Tran H., Hamada F., Schwarz-Romond T., Bienz M. Genes Dev. 22:528-542(2008) [PubMed: 18281465] [Abstract] Cited for: FUNCTION, INTERACTION WITH APC, DISRUPTION PHENOTYPE.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-770; SER-771 AND SER-775, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAF54429.1. AY058438 mRNA. Translation: AAL13667.1. AY069258 mRNA. Translation: AAL39403.1. Different initiation.
RefSeq	NP_649931.1. NM_141674.2.
UniGene	Dm.2210.
3D structure databases
ProteinModelPortal	Q9VH90.
SMR	Q9VH90. Positions 8-36, 89-114, 231-258.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-803912.
Proteomic databases
PRIDE	Q9VH90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0082091; FBpp0081569; FBgn0037734.
GeneID	41179.
KEGG	dme:Dmel_CG9448.
NMPDR	fig|7227.3.peg.11946.
UCSC	CG9448-RA. d. melanogaster.
Organism-specific databases
CTD	41179.
FlyBase	FBgn0037734. trbd.
Phylogenomic databases
eggNOG	inNOG05028.
GeneTree	EMGT00050000003550.
InParanoid	Q9VH90.
OMA	VENNYSA.
OrthoDB	EOG4RR4Z9.
PhylomeDB	Q9VH90.
Gene expression databases
ArrayExpress	Q9VH90.
Bgee	Q9VH90.
Family and domain databases
InterPro	IPR003323. OTU. IPR001876. Znf_RanBP2. [Graphical view]
KO	K11862.
Pfam	PF02338. OTU. 1 hit. PF00641. zf-RanBP. 1 hit. [Graphical view]
SMART	SM00547. ZnF_RBZ. 3 hits. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. PS01358. ZF_RANBP2_1. 3 hits. PS50199. ZF_RANBP2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	822587.

Entry information

Entry name

TRBID_DROME

Accession

Primary (citable) accession number: Q9VH90
Secondary accession number(s): Q8T9K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project