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Protein

Ubiquitin thioesterase trabid

Gene

trbd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positive regulator of the Wnt signaling pathway. Specifically cleaves 'Lys-63'-linked ubiquitin chains. May act by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription (By similarity). Required for an efficient wg response, but not for other signaling responses, in the eye.By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei518NucleophileBy similarity1
Active sitei658Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri5 – 36RanBP2-type 1PROSITE-ProRule annotationAdd BLAST32
Zinc fingeri89 – 118RanBP2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri232 – 261RanBP2-type 3PROSITE-ProRule annotationAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway, Wnt signaling pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-5689896 Ovarian tumor domain proteases

Protein family/group databases

MEROPSiC64.004

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase trabid (EC:3.4.19.12)
Short name:
dTrbd
Gene namesi
Name:trbd
ORF Names:CG9448
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037734 trbd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Flies are viable and fertile, suggesting functional redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003615591 – 778Ubiquitin thioesterase trabidAdd BLAST778

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei770Phosphoserine1 Publication1
Modified residuei771Phosphoserine1 Publication1
Modified residuei775Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VH90
PRIDEiQ9VH90

PTM databases

iPTMnetiQ9VH90

Expressioni

Gene expression databases

BgeeiFBgn0037734
GenevisibleiQ9VH90 DM

Interactioni

Subunit structurei

Interacts with Apc.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi66338, 12 interactors
IntActiQ9VH90, 11 interactors
STRINGi7227.FBpp0081569

Structurei

3D structure databases

ProteinModelPortaliQ9VH90
SMRiQ9VH90
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini507 – 665OTUPROSITE-ProRule annotationAdd BLAST159

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 230Gln-richAdd BLAST40

Domaini

The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

Sequence similaritiesi

Belongs to the peptidase C64 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri5 – 36RanBP2-type 1PROSITE-ProRule annotationAdd BLAST32
Zinc fingeri89 – 118RanBP2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri232 – 261RanBP2-type 3PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IQZM Eukaryota
ENOG410XS64 LUCA
GeneTreeiENSGT00530000062989
InParanoidiQ9VH90
KOiK11862
OMAiSEMKMDF
OrthoDBiEOG091G0495
PhylomeDBiQ9VH90

Family and domain databases

InterProiView protein in InterPro
IPR003323 OTU_dom
IPR001876 Znf_RanBP2
IPR036443 Znf_RanBP2_sf
PfamiView protein in Pfam
PF02338 OTU, 1 hit
PF00641 zf-RanBP, 1 hit
SMARTiView protein in SMART
SM00547 ZnF_RBZ, 3 hits
SUPFAMiSSF90209 SSF90209, 1 hit
PROSITEiView protein in PROSITE
PS50802 OTU, 1 hit
PS01358 ZF_RANBP2_1, 3 hits
PS50199 ZF_RANBP2_2, 3 hits

Sequencei

Sequence statusi: Complete.

Q9VH90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDTKDDAQK WKCETCTYEN YPSSLKCTMC QASKPLLNED IFRLSPAQES
60 70 80 90 100
CTVAEEAAAV EVAVMSPTPS STCYSLQPQS QARQSNVADS EKWPCKVCTY
110 120 130 140 150
LNWPRSLRCV QCCTKRGGEA IERGKKDMDN EADGDRAGEA LQALRISGSE
160 170 180 190 200
ENLANKPVQL IGATASHRLS LSRGIDDATH LNNLANASHN QSQSQHRQPV
210 220 230 240 250
LQQQMQLQLQ PQQQRESSSS AAVPPQQQKQ CYVSKWACNS CTYENWPRSI
260 270 280 290 300
KCSMCGKTRE REISGSQNDL HASSSLNSQE ENQQQLQQPN VDTVSVNNSF
310 320 330 340 350
NKKHIYQLGS SETINNCDTL QERQERRQRQ IRRQVDWQWL NACLGVVENN
360 370 380 390 400
YSAVEAYLSC GGNPARSLTS TEIAALNRNS AFDVGHTLIH LAIRFHREEM
410 420 430 440 450
LPMLLDQISG SGPGIKRVPS YVAPDLAADI RRHFANTLRL RKSGLPCHYV
460 470 480 490 500
QKHATFALPA EIEELPIPIQ EQLYDELLDR DAQKQLETPP PALNWSLEIT
510 520 530 540 550
ARLSSRMFVL WNRSAGDCLL DSAMQATWGV FDRDNILRRA LADTLHQCGH
560 570 580 590 600
VFFTRWKEYE MLQASMLHFT LEDSQFEEDW STLLSLAGQP GSSLEQLHIF
610 620 630 640 650
ALAHILRRPI IVYGVKYVKS FRGEDIGYAR FEGVYLPLFW DQNFCTKSPI
660 670 680 690 700
ALGYTRGHFS ALVPMEPFTR IDGRRDDVED VTYLPLMDCE LKLLPIHFLT
710 720 730 740 750
QSEVGNEESM MRQWLDVCVT DGGLLVAQQK LSKRPLLVAQ MLEEWLNHYR
760 770
RIAQVITAPF IRRPQITHYS SDGDSDEE
Length:778
Mass (Da):88,199
Last modified:May 1, 2000 - v1
Checksum:i57AAF0A860032F07
GO

Sequence cautioni

The sequence AAL39403 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA Translation: AAF54429.1
AY058438 mRNA Translation: AAL13667.1
AY069258 mRNA Translation: AAL39403.1 Different initiation.
RefSeqiNP_649931.1, NM_141674.3
UniGeneiDm.2210

Genome annotation databases

EnsemblMetazoaiFBtr0082091; FBpp0081569; FBgn0037734
GeneIDi41179
KEGGidme:Dmel_CG9448
UCSCiCG9448-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiTRBID_DROME
AccessioniPrimary (citable) accession number: Q9VH90
Secondary accession number(s): Q8T9K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health