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Protein

Ubiquitin thioesterase trabid

Gene

trbd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Positive regulator of the Wnt signaling pathway. Specifically cleaves 'Lys-63'-linked ubiquitin chains. May act by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription (By similarity). Required for an efficient wg response, but not for other signaling responses, in the eye.By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei518 – 5181NucleophileBy similarity
Active sitei658 – 6581Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 3632RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri89 – 11830RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri232 – 26130RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • positive regulation of Wnt signaling pathway Source: UniProtKB
  • protein deubiquitination Source: FlyBase
  • protein K63-linked deubiquitination Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC64.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase trabid (EC:3.4.19.12)
Short name:
dTrbd
Gene namesi
Name:trbd
ORF Names:CG9448
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037734. trbd.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Flies are viable and fertile, suggesting functional redundancy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778Ubiquitin thioesterase trabidPRO_0000361559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei770 – 7701Phosphoserine1 Publication
Modified residuei771 – 7711Phosphoserine1 Publication
Modified residuei775 – 7751Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VH90.
PRIDEiQ9VH90.

Expressioni

Gene expression databases

BgeeiQ9VH90.

Interactioni

Subunit structurei

Interacts with Apc.1 Publication

Protein-protein interaction databases

BioGridi66338. 9 interactions.
MINTiMINT-803912.

Structurei

3D structure databases

ProteinModelPortaliQ9VH90.
SMRiQ9VH90. Positions 470-717.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini507 – 665159OTUPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi191 – 23040Gln-richAdd
BLAST

Domaini

The OTU domain mediates the deubiquitinating activity.By similarity
The RanBP2-type zinc fingers mediate the specific interaction with 'Lys-63'-linked ubiquitin.By similarity

Sequence similaritiesi

Belongs to the peptidase C64 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 3 RanBP2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 3632RanBP2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri89 – 11830RanBP2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri232 – 26130RanBP2-type 3PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
InParanoidiQ9VH90.
KOiK11862.
OMAiSEMKMDF.
OrthoDBiEOG7R56SB.
PhylomeDBiQ9VH90.

Family and domain databases

Gene3Di4.10.1060.10. 1 hit.
InterProiIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VH90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDTKDDAQK WKCETCTYEN YPSSLKCTMC QASKPLLNED IFRLSPAQES
60 70 80 90 100
CTVAEEAAAV EVAVMSPTPS STCYSLQPQS QARQSNVADS EKWPCKVCTY
110 120 130 140 150
LNWPRSLRCV QCCTKRGGEA IERGKKDMDN EADGDRAGEA LQALRISGSE
160 170 180 190 200
ENLANKPVQL IGATASHRLS LSRGIDDATH LNNLANASHN QSQSQHRQPV
210 220 230 240 250
LQQQMQLQLQ PQQQRESSSS AAVPPQQQKQ CYVSKWACNS CTYENWPRSI
260 270 280 290 300
KCSMCGKTRE REISGSQNDL HASSSLNSQE ENQQQLQQPN VDTVSVNNSF
310 320 330 340 350
NKKHIYQLGS SETINNCDTL QERQERRQRQ IRRQVDWQWL NACLGVVENN
360 370 380 390 400
YSAVEAYLSC GGNPARSLTS TEIAALNRNS AFDVGHTLIH LAIRFHREEM
410 420 430 440 450
LPMLLDQISG SGPGIKRVPS YVAPDLAADI RRHFANTLRL RKSGLPCHYV
460 470 480 490 500
QKHATFALPA EIEELPIPIQ EQLYDELLDR DAQKQLETPP PALNWSLEIT
510 520 530 540 550
ARLSSRMFVL WNRSAGDCLL DSAMQATWGV FDRDNILRRA LADTLHQCGH
560 570 580 590 600
VFFTRWKEYE MLQASMLHFT LEDSQFEEDW STLLSLAGQP GSSLEQLHIF
610 620 630 640 650
ALAHILRRPI IVYGVKYVKS FRGEDIGYAR FEGVYLPLFW DQNFCTKSPI
660 670 680 690 700
ALGYTRGHFS ALVPMEPFTR IDGRRDDVED VTYLPLMDCE LKLLPIHFLT
710 720 730 740 750
QSEVGNEESM MRQWLDVCVT DGGLLVAQQK LSKRPLLVAQ MLEEWLNHYR
760 770
RIAQVITAPF IRRPQITHYS SDGDSDEE
Length:778
Mass (Da):88,199
Last modified:May 1, 2000 - v1
Checksum:i57AAF0A860032F07
GO

Sequence cautioni

The sequence AAL39403.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54429.1.
AY058438 mRNA. Translation: AAL13667.1.
AY069258 mRNA. Translation: AAL39403.1. Different initiation.
RefSeqiNP_649931.1. NM_141674.3.
UniGeneiDm.2210.

Genome annotation databases

EnsemblMetazoaiFBtr0082091; FBpp0081569; FBgn0037734.
GeneIDi41179.
KEGGidme:Dmel_CG9448.
UCSCiCG9448-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54429.1.
AY058438 mRNA. Translation: AAL13667.1.
AY069258 mRNA. Translation: AAL39403.1. Different initiation.
RefSeqiNP_649931.1. NM_141674.3.
UniGeneiDm.2210.

3D structure databases

ProteinModelPortaliQ9VH90.
SMRiQ9VH90. Positions 470-717.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66338. 9 interactions.
MINTiMINT-803912.

Protein family/group databases

MEROPSiC64.004.

Proteomic databases

PaxDbiQ9VH90.
PRIDEiQ9VH90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082091; FBpp0081569; FBgn0037734.
GeneIDi41179.
KEGGidme:Dmel_CG9448.
UCSCiCG9448-RA. d. melanogaster.

Organism-specific databases

CTDi41179.
FlyBaseiFBgn0037734. trbd.

Phylogenomic databases

eggNOGiNOG253754.
GeneTreeiENSGT00530000062989.
InParanoidiQ9VH90.
KOiK11862.
OMAiSEMKMDF.
OrthoDBiEOG7R56SB.
PhylomeDBiQ9VH90.

Miscellaneous databases

GenomeRNAii41179.
NextBioi822587.
PROiQ9VH90.

Gene expression databases

BgeeiQ9VH90.

Family and domain databases

Gene3Di4.10.1060.10. 1 hit.
InterProiIPR003323. OTU.
IPR001876. Znf_RanBP2.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00547. ZnF_RBZ. 3 hits.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
PS01358. ZF_RANBP2_1. 3 hits.
PS50199. ZF_RANBP2_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  4. "Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains."
    Tran H., Hamada F., Schwarz-Romond T., Bienz M.
    Genes Dev. 22:528-542(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH APC, DISRUPTION PHENOTYPE.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-770; SER-771 AND SER-775, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiTRBID_DROME
AccessioniPrimary (citable) accession number: Q9VH90
Secondary accession number(s): Q8T9K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.