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Protein

NAD-dependent protein deacetylase Sirt6

Gene

Sirt6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

NAD-dependent protein deacetylase (By similarity). May be involved in the regulation of life span.By similarity1 Publication

Catalytic activityi

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133Proton acceptorPROSITE-ProRule annotation1
Metal bindingi141ZincPROSITE-ProRule annotation1
Metal bindingi144ZincPROSITE-ProRule annotation1
Metal bindingi166ZincPROSITE-ProRule annotation1
Metal bindingi177ZincPROSITE-ProRule annotation1
Binding sitei259NAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 71NADBy similarityAdd BLAST20
Nucleotide bindingi113 – 116NADBy similarity4
Nucleotide bindingi215 – 217NADBy similarity3
Nucleotide bindingi241 – 243NADBy similarity3

GO - Molecular functioni

  • histone deacetylase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • NAD+ binding Source: InterPro

GO - Biological processi

  • determination of adult lifespan Source: FlyBase
  • histone deacetylation Source: FlyBase
  • positive regulation of chromatin silencing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacetylase Sirt6 (EC:3.5.1.-)
Alternative name(s):
Regulatory protein SIR2 homolog 6
SIR2-like protein 6
Gene namesi
Name:Sirt6
ORF Names:CG6284
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0037802. Sirt6.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Causes lethality during development. Induced silencing shortens life span.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173621 – 317NAD-dependent protein deacetylase Sirt6Add BLAST317

Proteomic databases

PaxDbiQ9VH08.
PRIDEiQ9VH08.

Expressioni

Gene expression databases

BgeeiFBgn0037802.
GenevisibleiQ9VH08. DM.

Interactioni

Protein-protein interaction databases

BioGridi66403. 3 interactors.
MINTiMINT-1576301.
STRINGi7227.FBpp0293897.

Structurei

3D structure databases

ProteinModelPortaliQ9VH08.
SMRiQ9VH08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 275Deacetylase sirtuin-typePROSITE-ProRule annotationAdd BLAST241

Sequence similaritiesi

Belongs to the sirtuin family. Class IV subfamily.Curated
Contains 1 deacetylase sirtuin-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1905. Eukaryota.
COG0846. LUCA.
InParanoidiQ9VH08.
KOiK11416.
OrthoDBiEOG091G0FOW.
PhylomeDBiQ9VH08.

Family and domain databases

Gene3Di3.40.50.1220. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 2 hits.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VH08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCNYADGLS AYDNKGILGA PESFDSDEVV AEKCQELAEL IKKSGHVVLH
60 70 80 90 100
TGAGISTSAG IPDFRGPKGV WTLEEKGEKP DFNVSFDEAR PTKTHMAIIA
110 120 130 140 150
LIESGYVQYV ISQNIDGLHL KSGLDRKYLS ELHGNIYIEQ CKKCRRQFVS
160 170 180 190 200
PSAVETVGQK SLQRACKSSM DSKGRSCRSG ILYDNVLDWE HDLPENDLEM
210 220 230 240 250
GVMHSTVADL NIALGTTLQI VPSGDLPLKN LKCGGKFVIC NLQPTKHDKK
260 270 280 290 300
ANLIISSYVD VVLSKVCKLL GVEIPEYSEA SDPTKQSKPM EWTIPTSNVN
310
TFHRQYKKYV YFIYYLL
Length:317
Mass (Da):35,204
Last modified:May 1, 2000 - v1
Checksum:i660B59F0EB3E3BAC
GO

Sequence cautioni

The sequence AAF54513 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AEB33520 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54513.2. Sequence problems.
BT126234 mRNA. Translation: AEB33520.1. Different initiation.
RefSeqiNP_649990.2. NM_141733.3.
UniGeneiDm.24872.

Genome annotation databases

GeneIDi41254.
KEGGidme:Dmel_CG6284.
UCSCiCG6284-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54513.2. Sequence problems.
BT126234 mRNA. Translation: AEB33520.1. Different initiation.
RefSeqiNP_649990.2. NM_141733.3.
UniGeneiDm.24872.

3D structure databases

ProteinModelPortaliQ9VH08.
SMRiQ9VH08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66403. 3 interactors.
MINTiMINT-1576301.
STRINGi7227.FBpp0293897.

Proteomic databases

PaxDbiQ9VH08.
PRIDEiQ9VH08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi41254.
KEGGidme:Dmel_CG6284.
UCSCiCG6284-RA. d. melanogaster.

Organism-specific databases

CTDi51548.
FlyBaseiFBgn0037802. Sirt6.

Phylogenomic databases

eggNOGiKOG1905. Eukaryota.
COG0846. LUCA.
InParanoidiQ9VH08.
KOiK11416.
OrthoDBiEOG091G0FOW.
PhylomeDBiQ9VH08.

Miscellaneous databases

ChiTaRSiSirt6. fly.
GenomeRNAii41254.
PROiQ9VH08.

Gene expression databases

BgeeiFBgn0037802.
GenevisibleiQ9VH08. DM.

Family and domain databases

Gene3Di3.40.50.1220. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERiPTHR11085. PTHR11085. 1 hit.
PfamiPF02146. SIR2. 2 hits.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIR6_DROME
AccessioniPrimary (citable) accession number: Q9VH08
Secondary accession number(s): F3YD73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.