Reviewed, UniProtKB/Swiss-Prot Q9VGZ0 (C12E1_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 12e1, mitochondrial
    EC=1.14.-.-
Alternative name(s):
    CYPXIIE1

Gene names

Name:	Cyp12e1
ORF Names:	CG14680

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VGZ0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q9VGZ0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     320-333: Missing.

Note: No experimental confirmation available.

Isoform C (identifier: Q9VGZ0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     21-21: R → RGLAQKATA

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 514		Probable cytochrome P450 12e1, mitochondrial		PRO_0000003614
Sites
	Metal binding	460	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	21	1	R → RGLAQKATA in isoform C.		VSP_027802
	Alternative sequence	320 – 333	14	Missing in isoform B.		VSP_027803

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified September 19, 2003. Version 3. Checksum: C9381850A54625D0	FASTA	514	58,943
	10 20 30 40 50 60 MLSTQWNANK QISRQIYQLC RVNLEEAKPY ADIPGPSKLQ LIRAFLPGGL YKNLPVHEMF 70 80 90 100 110 120 LDMNRQYGSI FRMPSVAGTD LVLTMNPQDY EVIFRNEGQY PYRRSFEVMD YFKRVHRREV 130 140 150 160 170 180 FDGYDGLTSG NGPAWGKMRT AVNPILLQPR NAKLYMTNLV QVSDEFLERI RIIRDPVTQE 190 200 210 220 230 240 MPDDFAVDIR HLVIESICSV ALNTHLGLLG EQRNNKDIQK LVLALQDVVE LGFQLDIMPA 250 260 270 280 290 300 FWKYLPMPNF KKLMRSLDTI TDFCYFHIGN ALKRIEEDAK AGTLNEIGLE TSLLEKLARF 310 320 330 340 350 360 DRQTAVIIAM DLLFAGADPT LVTLGGILFS LSKSPDKQAR LLEEIRGILP NKDSSLTIEN 370 380 390 400 410 420 MRNLPYLRAC IKEGIRMYPI GPGTLRRMPH DVVLSGYRVV AGTDVGIAAN YQMANMEQFV 430 440 450 460 470 480 PKVREFIPER WLRDESNSHL VGETATPFMY LPFGFGPRSC AGKRIVDMML EIAISRLVRN 490 500 510 FKIGFDYPIE NAFKAQFFVQ PNIPFKFKFI ERNE
	Isoform B. Checksum: 75B250AA5C9E2059	FASTA	500	57,513
	10 20 30 40 50 60 MLSTQWNANK QISRQIYQLC RVNLEEAKPY ADIPGPSKLQ LIRAFLPGGL YKNLPVHEMF 70 80 90 100 110 120 LDMNRQYGSI FRMPSVAGTD LVLTMNPQDY EVIFRNEGQY PYRRSFEVMD YFKRVHRREV 130 140 150 160 170 180 FDGYDGLTSG NGPAWGKMRT AVNPILLQPR NAKLYMTNLV QVSDEFLERI RIIRDPVTQE 190 200 210 220 230 240 MPDDFAVDIR HLVIESICSV ALNTHLGLLG EQRNNKDIQK LVLALQDVVE LGFQLDIMPA 250 260 270 280 290 300 FWKYLPMPNF KKLMRSLDTI TDFCYFHIGN ALKRIEEDAK AGTLNEIGLE TSLLEKLARF 310 320 330 340 350 360 DRQTAVIIAM DLLFAGADPS PDKQARLLEE IRGILPNKDS SLTIENMRNL PYLRACIKEG 370 380 390 400 410 420 IRMYPIGPGT LRRMPHDVVL SGYRVVAGTD VGIAANYQMA NMEQFVPKVR EFIPERWLRD 430 440 450 460 470 480 ESNSHLVGET ATPFMYLPFG FGPRSCAGKR IVDMMLEIAI SRLVRNFKIG FDYPIENAFK 490 500 AQFFVQPNIP FKFKFIERNE
	Isoform C. Checksum: 3831C5B03FC8FD16	FASTA	522	59,684
	10 20 30 40 50 60 MLSTQWNANK QISRQIYQLC RGLAQKATAV NLEEAKPYAD IPGPSKLQLI RAFLPGGLYK 70 80 90 100 110 120 NLPVHEMFLD MNRQYGSIFR MPSVAGTDLV LTMNPQDYEV IFRNEGQYPY RRSFEVMDYF 130 140 150 160 170 180 KRVHRREVFD GYDGLTSGNG PAWGKMRTAV NPILLQPRNA KLYMTNLVQV SDEFLERIRI 190 200 210 220 230 240 IRDPVTQEMP DDFAVDIRHL VIESICSVAL NTHLGLLGEQ RNNKDIQKLV LALQDVVELG 250 260 270 280 290 300 FQLDIMPAFW KYLPMPNFKK LMRSLDTITD FCYFHIGNAL KRIEEDAKAG TLNEIGLETS 310 320 330 340 350 360 LLEKLARFDR QTAVIIAMDL LFAGADPTLV TLGGILFSLS KSPDKQARLL EEIRGILPNK 370 380 390 400 410 420 DSSLTIENMR NLPYLRACIK EGIRMYPIGP GTLRRMPHDV VLSGYRVVAG TDVGIAANYQ 430 440 450 460 470 480 MANMEQFVPK VREFIPERWL RDESNSHLVG ETATPFMYLP FGFGPRSCAG KRIVDMMLEI 490 500 510 520 AISRLVRNFK IGFDYPIENA FKAQFFVQPN IPFKFKFIER NE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-514 (ISOFORM C). Strain: Berkeley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAF54532.3. AE014297 Genomic DNA. Translation: AAN13471.2. BT023136 mRNA. Translation: AAY55552.1. BT023153 mRNA. Translation: AAY55569.1.
RefSeq	NP_650003.3. NP_731481.2.
UniGene	Dm.24048
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0037817. Drosophila melanogaster. [Contig view]
GeneID	41272.
KEGG	dme:Dmel_CG14680.
NMPDR	fig|7227.3.peg.12076.
Organism-specific databases
FlyBase	FBgn0037817. Cyp12e1.
Phylogenomic databases
OMA	Q9VGZ0. ANYQMAN.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-010922-MON.
Gene expression databases
ArrayExpress	Q9VGZ0.
GermOnline	CG14680. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	823025.

Entry information

Entry name

C12E1_DROME

Accession

Primary (citable) accession number: Q9VGZ0
Secondary accession number(s): Q4V453, Q4V470, Q8INL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	September 19, 2003
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project