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Protein

DNA replication licensing factor Mcm5

Gene

Mcm5

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi378 – 3858ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • chromosome condensation Source: FlyBase
  • DNA endoreduplication Source: FlyBase
  • DNA replication initiation Source: InterPro
  • meiotic nuclear division Source: FlyBase
  • resolution of meiotic recombination intermediates Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor Mcm5 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 5 protein
Short name:
DmMCM5
Gene namesi
Name:Mcm5
ORF Names:CG4082
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0017577. Mcm5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi384 – 3841K → A: Greatly reduces complex helicase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 733733DNA replication licensing factor Mcm5PRO_0000406422Add
BLAST

Proteomic databases

PaxDbiQ9VGW6.
PRIDEiQ9VGW6.

Expressioni

Gene expression databases

BgeeiFBgn0017577.
GenevisibleiQ9VGW6. DM.

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mcm2P497353EBI-83298,EBI-138228
Mcm3Q9XYU13EBI-83298,EBI-103930

Protein-protein interaction databases

BioGridi66440. 17 interactions.
IntActiQ9VGW6. 17 interactions.
MINTiMINT-983807.
STRINGi7227.FBpp0081756.

Structurei

3D structure databases

ProteinModelPortaliQ9VGW6.
SMRiQ9VGW6. Positions 4-684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini328 – 534207MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi509 – 5124Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000074928.
InParanoidiQ9VGW6.
KOiK02209.
OMAiIVKDTHD.
OrthoDBiEOG091G027N.
PhylomeDBiQ9VGW6.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VGW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGFDDAGVF FSDNFGGDNQ QDAQINLQAV KKKYKEFIRT FNEENFFYKY
60 70 80 90 100
RDTLKRNYLN GRYFLEIEME DLVGFDETLA DKLNKQPTEH LEIFEEAARE
110 120 130 140 150
VADEITAPRP EHEEHMHDIQ ILLSSNANPT NIRQLKSDCV SKLVKIAGII
160 170 180 190 200
VAASGISAKA TRMSIQCLSC STVIPNLKVN PGLEGYALPR KCNTEQAGRP
210 220 230 240 250
KCPLDPFFIM PDKCKCVDFQ TLKLQELPDF VPQGEIPRHL QLFCDRSLCE
260 270 280 290 300
RVVPGNRVLI QGIYSIRKVG KPSRRDGREK AVVGVRAPYM RVVGITVDSE
310 320 330 340 350
GAGAISRYSN ITSDEEEHFR RMAASGDIYE RLSQSLAPSI FGSRDIKKAI
360 370 380 390 400
TCMLFGGSRK RLPDGLCRRG DINVLLLGDP GTAKSQLLKF VEKVAPIAVY
410 420 430 440 450
TSGKGSSAAG LTASVMKDPQ TRNFVMEGGA MVLADGGVVC IDEFDKMRED
460 470 480 490 500
DRVAIHEAME QQTISIAKAG ITTTLNSRCS VLAAANSIFG RWDDTKGEEN
510 520 530 540 550
IDFMPTILSR FDMIFIVKDI HDESRDITLA KHIINVHLSS NKSAPSEPAE
560 570 580 590 600
GEISLSTFKK YIHYCRTHCG PRLSEAAGEK LKSRYVLMRS GAGQQEKASD
610 620 630 640 650
KRLSIPITVR QLEAVIRISE SLAKIRLQPF ATDEHVNEAL RLFQVSTLDA
660 670 680 690 700
AMTGSLAGAE GFTTEEDQET LNRIEKQLKR RFAIGSQVSE QNILQDFLRQ
710 720 730
KYEERTVMKV IHTMIRRGEL QHRMQRKMLY RIC
Length:733
Mass (Da):82,258
Last modified:May 1, 2000 - v1
Checksum:i36FEB693045A30B8
GO

Sequence cautioni

The sequence AAC47652 differs from that shown. Reason: Frameshift at positions 82 and 84. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021A → V in AAC47652 (PubMed:10731132).Curated
Sequence conflicti319 – 3191F → L in AAC47652 (PubMed:10731132).Curated
Sequence conflicti357 – 3571G → V in AAC47652 (PubMed:10731132).Curated
Sequence conflicti426 – 4261M → V in AAC47652 (PubMed:10731132).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83493 mRNA. Translation: AAC47652.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF54557.1.
AY071628 mRNA. Translation: AAL49250.1.
RefSeqiNP_524308.2. NM_079584.4.
UniGeneiDm.5829.

Genome annotation databases

EnsemblMetazoaiFBtr0082279; FBpp0081756; FBgn0017577.
GeneIDi41296.
KEGGidme:Dmel_CG4082.
UCSCiCG4082-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83493 mRNA. Translation: AAC47652.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF54557.1.
AY071628 mRNA. Translation: AAL49250.1.
RefSeqiNP_524308.2. NM_079584.4.
UniGeneiDm.5829.

3D structure databases

ProteinModelPortaliQ9VGW6.
SMRiQ9VGW6. Positions 4-684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66440. 17 interactions.
IntActiQ9VGW6. 17 interactions.
MINTiMINT-983807.
STRINGi7227.FBpp0081756.

Proteomic databases

PaxDbiQ9VGW6.
PRIDEiQ9VGW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082279; FBpp0081756; FBgn0017577.
GeneIDi41296.
KEGGidme:Dmel_CG4082.
UCSCiCG4082-RA. d. melanogaster.

Organism-specific databases

CTDi4174.
FlyBaseiFBgn0017577. Mcm5.

Phylogenomic databases

eggNOGiKOG0481. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000074928.
InParanoidiQ9VGW6.
KOiK02209.
OMAiIVKDTHD.
OrthoDBiEOG091G027N.
PhylomeDBiQ9VGW6.

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Miscellaneous databases

GenomeRNAii41296.
PROiQ9VGW6.

Gene expression databases

BgeeiFBgn0017577.
GenevisibleiQ9VGW6. DM.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008048. MCM5.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01661. MCMPROTEIN5.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM5_DROME
AccessioniPrimary (citable) accession number: Q9VGW6
Secondary accession number(s): P91676
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.