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Protein

Cytochrome P450 315a1, mitochondrial

Gene

sad

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.1 Publication

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.1 Publication

Cofactori

hemeBy similarity

Pathwayi: ecdysteroid biosynthesis

This protein is involved in the pathway ecdysteroid biosynthesis, which is part of Steroid biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway ecdysteroid biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi466Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • central nervous system development Source: FlyBase
  • dorsal closure Source: FlyBase
  • ecdysone biosynthetic process Source: FlyBase
  • head involution Source: FlyBase
  • midgut development Source: FlyBase
  • motor neuron axon guidance Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18113.
ReactomeiR-DME-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-DME-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-DME-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-DME-193993. Mineralocorticoid biosynthesis.
R-DME-194002. Glucocorticoid biosynthesis.
R-DME-196108. Pregnenolone biosynthesis.
R-DME-196791. Vitamin D (calciferol) metabolism.
R-DME-211916. Vitamins.
R-DME-211976. Endogenous sterols.
UniPathwayiUPA00765.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 315a1, mitochondrial (EC:1.14.99.-)
Alternative name(s):
CYPCCCXVA1
Protein shadow
Gene namesi
Name:sad
Synonyms:Cyp315a1
ORF Names:CG14728
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003312. sad.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial membrane Source: UniProtKB-SubCell
  • mitochondrion Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000003631? – 520Cytochrome P450 315a1, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ9VGH1.
PRIDEiQ9VGH1.

Expressioni

Tissue specificityi

Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.1 Publication

Gene expression databases

BgeeiFBgn0003312.
GenevisibleiQ9VGH1. DM.

Interactioni

Protein-protein interaction databases

BioGridi69335. 1 interactor.
STRINGi7227.FBpp0081949.

Structurei

3D structure databases

ProteinModelPortaliQ9VGH1.
SMRiQ9VGH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000119243.
InParanoidiQ9VGH1.
KOiK10722.
OMAiRIFVDLV.
OrthoDBiEOG091G0E2I.
PhylomeDBiQ9VGH1.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VGH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKRERPGP LRWLRHLLDQ LLVRILSLSL FRSRCDPPPL QRFPATELPP
60 70 80 90 100
AVAAKYVPIP RVKGLPVVGT LVDLIAAGGA THLHKYIDAR HKQYGPIFRE
110 120 130 140 150
RLGGTQDAVF VSSANLMRGV FQHEGQYPQH PLPDAWTLYN QQHACQRGLF
160 170 180 190 200
FMEGAEWLHN RRILNRLLLN GNLNWMDVHI ESCTRRMVDQ WKRRTAEAAA
210 220 230 240 250
IPLAESGEIR SYELPLLEQQ LYRWSIEVLC CIMFGTSVLT CPKIQSSLDY
260 270 280 290 300
FTQIVHKVFE HSSRLMTFPP RLAQILRLPI WRDFEANVDE VLREGAAIID
310 320 330 340 350
HCIRVQEDQR RPHDEALYHR LQAADVPGDM IKRIFVDLVI AAGDTTAFSS
360 370 380 390 400
QWALFALSKE PRLQQRLAKE RATNDSRLMH GLIKESLRLY PVAPFIGRYL
410 420 430 440 450
PQDAQLGGHF IEKDTMVLLS LYTAGRDPSH FEQPERVLPE RWCIGETEQV
460 470 480 490 500
HKSHGSLPFA IGQRSCIGRR VALKQLHSLL GRCAAQFEMS CLNEMPVDSV
510 520
LRMVTVPDRT LRLALRPRTE
Length:520
Mass (Da):59,631
Last modified:May 1, 2000 - v1
Checksum:i6DC4A2CCD8E07BC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti484A → T in AAL86019 (PubMed:12177427).Curated1
Sequence conflicti509R → Q in AAL86019 (PubMed:12177427).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY079170 mRNA. Translation: AAL86019.1.
AE014297 Genomic DNA. Translation: AAF54711.1.
BT016061 mRNA. Translation: AAV36946.1.
RefSeqiNP_650123.1. NM_141866.2.
UniGeneiDm.14394.

Genome annotation databases

EnsemblMetazoaiFBtr0082475; FBpp0081949; FBgn0003312.
GeneIDi44858.
KEGGidme:Dmel_CG14728.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY079170 mRNA. Translation: AAL86019.1.
AE014297 Genomic DNA. Translation: AAF54711.1.
BT016061 mRNA. Translation: AAV36946.1.
RefSeqiNP_650123.1. NM_141866.2.
UniGeneiDm.14394.

3D structure databases

ProteinModelPortaliQ9VGH1.
SMRiQ9VGH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi69335. 1 interactor.
STRINGi7227.FBpp0081949.

Proteomic databases

PaxDbiQ9VGH1.
PRIDEiQ9VGH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0082475; FBpp0081949; FBgn0003312.
GeneIDi44858.
KEGGidme:Dmel_CG14728.

Organism-specific databases

CTDi44858.
FlyBaseiFBgn0003312. sad.

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000119243.
InParanoidiQ9VGH1.
KOiK10722.
OMAiRIFVDLV.
OrthoDBiEOG091G0E2I.
PhylomeDBiQ9VGH1.

Enzyme and pathway databases

UniPathwayiUPA00765.
BioCyciMetaCyc:MONOMER-18113.
ReactomeiR-DME-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-DME-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-DME-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-DME-193993. Mineralocorticoid biosynthesis.
R-DME-194002. Glucocorticoid biosynthesis.
R-DME-196108. Pregnenolone biosynthesis.
R-DME-196791. Vitamin D (calciferol) metabolism.
R-DME-211916. Vitamins.
R-DME-211976. Endogenous sterols.

Miscellaneous databases

GenomeRNAii44858.
PROiQ9VGH1.

Gene expression databases

BgeeiFBgn0003312.
GenevisibleiQ9VGH1. DM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCP315_DROME
AccessioniPrimary (citable) accession number: Q9VGH1
Secondary accession number(s): Q5U131, Q8MTR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Member of the Halloween gene group.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.