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Protein

Glutathione S-transferase D2

Gene

GstD2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in detoxification (PubMed:22082028).1 Publication

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.1 Publication

Kineticsi

  1. KM=1.84 mM for glutathione1 Publication
  2. KM=0.81 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=0.53 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication
  2. Vmax=0.50 µmol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate1 Publication
  3. Vmax=0.013 µmol/min/mg enzyme with phenethyl isothiocyanate as substrate1 Publication

GO - Molecular functioni

  • glutathione peroxidase activity Source: FlyBase
  • glutathione transferase activity Source: FlyBase

GO - Biological processi

  • glutathione metabolic process Source: FlyBase

Keywordsi

Molecular functionTransferase
Biological processDetoxification

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase D21 Publication (EC:2.5.1.181 Publication)
Gene namesi
Name:GstD2Imported
Synonyms:GSTD2-2, gstD21Imported
ORF Names:CG4181Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010038. GstD2.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859541 – 215Glutathione S-transferase D2Add BLAST215

Proteomic databases

PaxDbiQ9VG98.
PRIDEiQ9VG98.

Expressioni

Gene expression databases

BgeeiFBgn0010038.
ExpressionAtlasiQ9VG98. differential.
GenevisibleiQ9VG98. DM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi71332. 6 interactors.
STRINGi7227.FBpp0082041.

Structurei

Secondary structure

1215
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 5Combined sources4
Helixi10 – 21Combined sources12
Beta strandi27 – 30Combined sources4
Helixi33 – 35Combined sources3
Helixi37 – 39Combined sources3
Helixi41 – 46Combined sources6
Beta strandi52 – 57Combined sources6
Beta strandi60 – 64Combined sources5
Helixi65 – 76Combined sources12
Turni79 – 81Combined sources3
Helixi87 – 102Combined sources16
Helixi104 – 112Combined sources9
Helixi124 – 140Combined sources17
Turni141 – 143Combined sources3
Beta strandi149 – 151Combined sources3
Helixi154 – 168Combined sources15
Helixi177 – 189Combined sources13
Helixi193 – 211Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F0GX-ray1.60A/B1-215[»]
ProteinModelPortaliQ9VG98.
SMRiQ9VG98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80GST N-terminalAdd BLAST80
Domaini86 – 212GST C-terminalAdd BLAST127

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni50 – 52Glutathione bindingBy similarity3
Regioni64 – 66Glutathione bindingBy similarity3

Sequence similaritiesi

Belongs to the GST superfamily. Delta family.1 Publication

Phylogenomic databases

eggNOGiKOG0867. Eukaryota.
COG0625. LUCA.
InParanoidiQ9VG98.
KOiK00799.
OMAiPGWDENE.
OrthoDBiEOG091G0MS3.
PhylomeDBiQ9VG98.

Family and domain databases

InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
PfamiView protein in Pfam
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VG98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFYYMPGGG GCRTVIMVAK ALGLELNKKL LNTMEGEQLK PEFVKLNPQH
60 70 80 90 100
TIPTLVDNGF SIWESRAIAV YLVEKYGKDD YLLPNDPKKR AVINQRLYFD
110 120 130 140 150
MGTLYESFAK YYYPLFRTGK PGSDEDLKRI ETAFGFLDTF LEGQEYVAGD
160 170 180 190 200
QLTVADIAIL STVSTFEVSE FDFSKYSNVS RWYDNAKKVT PGWDENWEGL
210
MAMKALFDAR KLAAK
Length:215
Mass (Da):24,535
Last modified:May 1, 2000 - v1
Checksum:i221A9961BBF0E5B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97702 Genomic DNA. No translation available.
AE014297 Genomic DNA. Translation: AAF54787.1.
PIRiD46681.
RefSeqiNP_524912.1. NM_080173.2.
UniGeneiDm.1756.

Genome annotation databases

EnsemblMetazoaiFBtr0082569; FBpp0082041; FBgn0010038.
GeneIDi48335.
KEGGidme:Dmel_CG4181.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGSTD2_DROME
AccessioniPrimary (citable) accession number: Q9VG98
Secondary accession number(s): Q9TX91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families