Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

26S proteasome non-ATPase regulatory subunit 9

Gene

CG9588

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC).By similarity

GO - Biological processi

  • cellular response to DNA damage stimulus Source: FlyBase
  • proteasome regulatory particle assembly Source: GO_Central

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 9By similarity
Alternative name(s):
26S proteasome regulatory subunit p27By similarity
Short name:
dp271 Publication
Gene namesi
ORF Names:CG9588
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038166. CG9588.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9E → A: Abolishes interaction with PI31. 1 Publication1
Mutagenesisi10R → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi11L → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi114P → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi121D → A: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi185V → T: Does not affect interaction with PI31. 1 Publication1
Mutagenesisi187R → A: Abolishes interaction with PI31. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004248931 – 22026S proteasome non-ATPase regulatory subunit 9Add BLAST220

Proteomic databases

PaxDbiQ9VFS8.
PRIDEiQ9VFS8.

Expressioni

Gene expression databases

BgeeiFBgn0038166.
GenevisibleiQ9VFS8. DM.

Interactioni

Subunit structurei

Interacts with PI31; this interaction is increased by PI31 ADP-ribosylation. Interacts with Rpt5.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PI31Q9V6373EBI-166054,EBI-144377

Protein-protein interaction databases

BioGridi66752. 13 interactors.
IntActiQ9VFS8. 11 interactors.
MINTiMINT-827040.
STRINGi7227.FBpp0082314.

Structurei

3D structure databases

ProteinModelPortaliQ9VFS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 200PDZSequence analysisAdd BLAST99

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili4 – 32Sequence analysisAdd BLAST29
Coiled coili61 – 91Sequence analysisAdd BLAST31

Sequence similaritiesi

Belongs to the proteasome subunit p27 family.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3129. Eukaryota.
COG0265. LUCA.
InParanoidiQ9VFS8.
KOiK06693.
OMAiLGCNIVP.
OrthoDBiEOG091G0N53.
PhylomeDBiQ9VFS8.

Family and domain databases

InterProiView protein in InterPro
IPR001478. PDZ.
PfamiView protein in Pfam
PF13180. PDZ_2. 1 hit.
SMARTiView protein in SMART
SM00228. PDZ. 1 hit.
SUPFAMiSSF50156. SSF50156. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VFS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGTTTKER LERLINAKKQ LEAQINRNGQ ILAANDNVGM SGPLVDAEGF
60 70 80 90 100
PRNDIDVYQV RLARQTIICL QNDHKELMNQ IQTLLNQYHS EIATTDPELV
110 120 130 140 150
NRASALDLDS DRSPGGANIT DLAPARAIVV VNLVSPDSPA ERAGLCAGDA
160 170 180 190 200
ILRFGSINSG NFKGDLAQIG ELVRNMQSQN VQLKVKRGEQ QLDLILVPKT
210 220
WSGRGLLGCN IVLPPEAMDH
Length:220
Mass (Da):23,898
Last modified:May 1, 2000 - v1
Checksum:iC618E8D04262293A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF54971.1.
AY071064 mRNA. Translation: AAL48686.1.
RefSeqiNP_650301.1. NM_142044.4.
UniGeneiDm.20184.

Genome annotation databases

EnsemblMetazoaiFBtr0082849; FBpp0082314; FBgn0038166.
GeneIDi41672.
KEGGidme:Dmel_CG9588.
UCSCiCG9588-RA. d. melanogaster.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPSMD9_DROME
AccessioniPrimary (citable) accession number: Q9VFS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 22, 2014
Last sequence update: May 1, 2000
Last modified: July 5, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families