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Protein

Carotenoid isomerooxygenase

Gene

ninaB

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative cleavage at the 15,15'-double bond of carotenoids and the simultaneous all-trans to 11-cis isomerization of one cleavage product. Carotenoids like 11-cis retinal can promote visual pigment biogenesis in the dark. Essential for the biosynthesis of the 3-hydroxyretinal chromophore of rhodopsin from zeaxanthin and for proper photoreceptor development. Also essential for larval light perception.5 Publications

Catalytic activityi

Zeaxanthin + O2 = (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal.2 Publications

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Pathwayi: retinol metabolism

This protein is involved in the pathway retinol metabolism, which is part of Cofactor metabolism.
View all proteins of this organism that are known to be involved in the pathway retinol metabolism and in Cofactor metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi211Iron; catalyticBy similarity1
Metal bindingi267Iron; catalyticBy similarity1
Metal bindingi337Iron; catalyticBy similarity1
Metal bindingi612Iron; catalyticBy similarity1

GO - Molecular functioni

  • beta-carotene 15,15'-monooxygenase activity Source: FlyBase
  • carotenoid dioxygenase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • monooxygenase activity Source: UniProtKB-KW
  • retinal isomerase activity Source: FlyBase

GO - Biological processi

  • carotene metabolic process Source: FlyBase
  • oxidation-reduction process Source: FlyBase
  • phototransduction Source: FlyBase
  • phototransduction, UV Source: FlyBase
  • phototransduction, visible light Source: FlyBase
  • retinal metabolic process Source: FlyBase
  • retinol metabolic process Source: UniProtKB-UniPathway
  • rhodopsin biosynthetic process Source: FlyBase
  • vitamin A biosynthetic process Source: FlyBase
  • vitamin biosynthetic process Source: FlyBase

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17370.
BRENDAi1.13.11.65. 1994.
1.14.99.36. 1994.
ReactomeiR-DME-2453902. The canonical retinoid cycle in rods (twilight vision).
R-DME-975634. Retinoid metabolism and transport.
UniPathwayiUPA00912.

Names & Taxonomyi

Protein namesi
Recommended name:
Carotenoid isomerooxygenase (EC:1.13.11.65)
Alternative name(s):
Beta-carotene 15,15'-monooxygenase and retinoid isomerase
Beta-carotene dioxygenase and retinoid isomerase
Neither inactivation nor afterpotential mutant B
Gene namesi
Name:ninaB
Synonyms:beta-diox
ORF Names:CG9347
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0002937. ninaB.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi280E → K in ninaB(P315); abolishes catalytic activity. 1 Publication1
Mutagenesisi471M → L in ninaB(P315); no effect. 1 Publication1
Mutagenesisi477E → A in ninaB(P315); no effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004241571 – 620Carotenoid isomerooxygenaseAdd BLAST620

Proteomic databases

PaxDbiQ9VFS2.
PRIDEiQ9VFS2.

Expressioni

Tissue specificityi

Expression follows organogenesis of the larval Bolwig's organ (BO), which mediates larval photophobic behavior. In the adult, expression is restricted exclusively to the brain. Expressed in both neuronal cells and glia cells. Not active within photoreceptors. Active within neuronal cells within the central nervous system.5 Publications

Gene expression databases

BgeeiFBgn0002937.
ExpressionAtlasiQ9VFS2. differential.
GenevisibleiQ9VFS2. DM.

Interactioni

Protein-protein interaction databases

IntActiQ9VFS2. 1 interactor.
STRINGi7227.FBpp0082288.

Structurei

3D structure databases

ProteinModelPortaliQ9VFS2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carotenoid oxygenase family.Curated

Phylogenomic databases

eggNOGiKOG1285. Eukaryota.
COG3670. LUCA.
GeneTreeiENSGT00500000044783.
InParanoidiQ9VFS2.
KOiK18048.
OMAiSMISIYP.
OrthoDBiEOG091G05GM.
PhylomeDBiQ9VFS2.

Family and domain databases

InterProiView protein in InterPro
IPR004294. Carotenoid_Oase.
PANTHERiPTHR10543. PTHR10543. 1 hit.
PfamiView protein in Pfam
PF03055. RPE65. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VFS2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGVFKSFM RDFFAVKYDE QRNDPQAERL DGNGRLYPNC SSDVWLRSCE
60 70 80 90 100
REIVDPIEGH HSGHIPKWIC GSLLRNGPGS WKVGDMTFGH LFDCSALLHR
110 120 130 140 150
FAIRNGRVTY QNRFVDTETL RKNRSAQRIV VTEFGTAAVP DPCHSIFDRF
160 170 180 190 200
AAIFRPDSGT DNSMISIYPF GDQYYTFTET PFMHRINPCT LATEARICTT
210 220 230 240 250
DFVGVVNHTS HPHVLPSGTV YNLGTTMTRS GPAYTILSFP HGEQMFEDAH
260 270 280 290 300
VVATLPCRWK LHPGYMHTFG LTDHYFVIVE QPLSVSLTEY IKAQLGGQNL
310 320 330 340 350
SACLKWFEDR PTLFHLIDRV SGKLVQTYES EAFFYLHIIN CFERDGHVVV
360 370 380 390 400
DICSYRNPEM INCMYLEAIA NMQTNPNYAT LFRGRPLRFV LPLGTIPPAS
410 420 430 440 450
IAKRGLVKSF SLAGLSAPQV SRTMKHSVSQ YADITYMPTN GKQATAGEES
460 470 480 490 500
PKRDAKRGRY EEENLVNLVT MEGSQAEAFQ GTNGIQLRPE MLCDWGCETP
510 520 530 540 550
RIYYERYMGK NYRYFYAISS DVDAVNPGTL IKVDVWNKSC LTWCEENVYP
560 570 580 590 600
SEPIFVPSPD PKSEDDGVIL ASMVLGGLND RYVGLIVLCA KTMTELGRCD
610 620
FHTNGPVPKC LHGWFAPNAI
Length:620
Mass (Da):69,932
Last modified:May 1, 2000 - v1
Checksum:i91EC22EB102C31C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276682 mRNA. Translation: CAB93141.1.
AE014297 Genomic DNA. Translation: AAF54978.1.
AY121617 mRNA. Translation: AAM51944.1.
RefSeqiNP_650307.2. NM_142050.3.
UniGeneiDm.10048.

Genome annotation databases

EnsemblMetazoaiFBtr0082820; FBpp0082288; FBgn0002937.
GeneIDi41678.
KEGGidme:Dmel_CG9347.
UCSCiCG9347-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiNINAB_DROME
AccessioniPrimary (citable) accession number: Q9VFS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families