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Protein

Protein roadkill

Gene

rdx

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in segment polarity. In complex with gft/CUL3, promotes ubiquitination of ci and its subsequent degradation by the proteasome, which results in hh signaling attenuation. This regulation may be important during eye formation for proper packing of ommatidia into a hexagonal array.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: FlyBase
  • ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  • establishment of ommatidial planar polarity Source: FlyBase
  • eye development Source: UniProtKB
  • eye morphogenesis Source: FlyBase
  • negative regulation of protein import into nucleus Source: FlyBase
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • positive regulation of apoptotic process Source: FlyBase
  • positive regulation of JNK cascade Source: FlyBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein autoubiquitination Source: FlyBase
  • protein destabilization Source: FlyBase
  • protein polyubiquitination involved in nucleus-associated proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
  • protein ubiquitination Source: UniProtKB
  • regulation of proteolysis Source: FlyBase
  • segment polarity determination Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Segmentation polarity protein
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-DME-216167 Nuclear CI is degraded
R-DME-5632684 Hedgehog 'on' state
SignaLinkiQ9VFP2
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Protein roadkill
Alternative name(s):
Hh-induced MATH and BTB domain-containing protein
Gene namesi
Name:rdx
Synonyms:HIB
ORF Names:CG12537
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0264493 rdx

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002745931 – 829Protein roadkillAdd BLAST829

Proteomic databases

PaxDbiQ9VFP2
PRIDEiQ9VFP2

Expressioni

Tissue specificityi

Expressed near the anterio-posterior compartment boundary of antenna, leg and wing disks.1 Publication

Developmental stagei

Expressed maternally in stage 1 embryos. Isoform 1 and isoform 4 are expressed zygotically in stage 4 in pole cells. By stage 8, expressed in 14 segmentally repeating stripes. During stage 9 and 10, expression appears in neuroblasts. After stage 14, expression is restricted to clypeolabrum, anal plate and salivary glands.1 Publication

Inductioni

By hh during segmentation.2 Publications

Gene expression databases

BgeeiFBgn0264493
ExpressionAtlasiQ9VFP2 baseline and differential
GenevisibleiQ9VFP2 DM

Interactioni

Subunit structurei

Interacts with ci and gft/CUL3.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: FlyBase
  • ubiquitin protein ligase binding Source: GO_Central

Protein-protein interaction databases

BioGridi66781, 50 interactors
DIPiDIP-29550N
IntActiQ9VFP2, 11 interactors
STRINGi7227.FBpp0099767

Structurei

3D structure databases

ProteinModelPortaliQ9VFP2
SMRiQ9VFP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini486 – 616MATHPROSITE-ProRule annotationAdd BLAST131
Domaini655 – 722BTBPROSITE-ProRule annotationAdd BLAST68

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 36Poly-Gln6
Compositional biasi82 – 86Poly-Ser5
Compositional biasi109 – 145Gln-richAdd BLAST37
Compositional biasi156 – 195Ala-richAdd BLAST40
Compositional biasi208 – 395Ser-richAdd BLAST188
Compositional biasi289 – 387His-richAdd BLAST99

Domaini

The MATH domain interacts with ci.
The BTB (POZ) domain interacts with gft/CUL3.

Sequence similaritiesi

Belongs to the Tdpoz family.Curated

Phylogenomic databases

eggNOGiKOG1987 Eukaryota
ENOG410XQV8 LUCA
GeneTreeiENSGT00390000000361
InParanoidiQ9VFP2
KOiK10523
OrthoDBiEOG091G07ZM
PhylomeDBiQ9VFP2

Family and domain databases

CDDicd14734 SPOP_C, 1 hit
Gene3Di2.60.210.10, 1 hit
InterProiView protein in InterPro
IPR000210 BTB/POZ_dom
IPR002083 MATH/TRAF_dom
IPR011333 SKP1/BTB/POZ_sf
IPR034089 SPOP_C
IPR008974 TRAF-like
PfamiView protein in Pfam
PF00651 BTB, 1 hit
PF00917 MATH, 1 hit
SMARTiView protein in SMART
SM00225 BTB, 1 hit
SM00061 MATH, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit
PS50144 MATH, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q9VFP2-1) [UniParc]FASTAAdd to basket
Also known as: EImported

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFEPYVKIKK KRSVNEIYEN ENLEQQQHHL QQQQQQPATS DNCCCENGEP
60 70 80 90 100
QNAPEVATAT VAATSVAATS AAATVATGAA ASSSSSGNCS RLQQLISTPP
110 120 130 140 150
VLLRRSSLQQ QQHQQQQHHP HTPAATATPP QQQQQQQAAP SVLQQHLGHL
160 170 180 190 200
NYESGATAAA AATAAAAAAT VATSRSGSAT LAQHLATPSN ILQAAFGSSN
210 220 230 240 250
LQHILTRSAP SPSSSAISSN NCSSACAGNT HYNGGNSNSG SSSSNSNHHS
260 270 280 290 300
NSIIASRLFG AASSSSSSSS SSASASSSSV AASSSSSSHH LHSHHSALTN
310 320 330 340 350
SITNRINQSI RRHLNQQQHH HPLSASSSSA SASPSASTSS SSSYQQSSVQ
360 370 380 390 400
QQHYNCAHPA QQQQHHHHHH SSSSSSSSSS SSSHHHHNSS SSSNSNNQQQ
410 420 430 440 450
PQQSPLCLVL LVKCPNSKEF CNAAANFCDK RLPVNECQAS QTARVTSNLH
460 470 480 490 500
ASSSTMAVSR VPSPPLPEVN TPVAENWCYT QVKVVKFSYM WTINNFSFCR
510 520 530 540 550
EEMGEVLKSS TFSAGANDKL KWCLRVNPKG LDEESKDYLS LYLLLVSCNK
560 570 580 590 600
SEVRAKFKFS ILNAKREETK AMESQRAYRF VQGKDWGFKK FIRRDFLLDE
610 620 630 640 650
ANGLLPEDKL TIFCEVSVVA DSVNISGQSN IVQFKVPECK LSEDLGNLFD
660 670 680 690 700
NEKFSDVTLS VGGREFQAHK AILAARSDVF AAMFEHEMEE RKLNRVAITD
710 720 730 740 750
VDHEVLKEML RFIYTGKAPN LEKMADDLLA AADKYALEKL KVMCEEALCV
760 770 780 790 800
NLSVETAAET LILADLHSAD QLKAQTIDFI NTHATDVMET SGWQNMITTH
810 820
SHLIAEAFRA LATQQIPPIG PPRKRVKMS
Length:829
Mass (Da):89,667
Last modified:February 7, 2006 - v2
Checksum:i608D32B45859280E
GO
Isoform 2Curated (identifier: Q9VFP2-2) [UniParc]FASTAAdd to basket
Also known as: BImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-455: Missing.

Show »
Length:374
Mass (Da):42,284
Checksum:i2116548EE64F04C0
GO
Isoform 3Curated (identifier: Q9VFP2-3) [UniParc]FASTAAdd to basket
Also known as: CImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-426: Missing.
     427-430: FCDK → MALA

Show »
Length:403
Mass (Da):45,310
Checksum:i190BB5EC3B108CD8
GO
Isoform 4Curated (identifier: Q9VFP2-4) [UniParc]FASTAAdd to basket
Also known as: AImported, FImported

The sequence of this isoform differs from the canonical sequence as follows:
     1-423: Missing.
     424-430: AANFCDK → MDLIQEP

Show »
Length:406
Mass (Da):45,750
Checksum:i344A5EA7A271D404
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti536K → T in ABG24573 (PubMed:16651542).Curated1
Sequence conflicti716G → D in ABG24573 (PubMed:16651542).Curated1
Sequence conflicti749 – 750CV → WL in ABG24573 (PubMed:16651542).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0228251 – 455Missing in isoform 2. 1 PublicationAdd BLAST455
Alternative sequenceiVSP_0228261 – 426Missing in isoform 3. 1 PublicationAdd BLAST426
Alternative sequenceiVSP_0228271 – 423Missing in isoform 4. CuratedAdd BLAST423
Alternative sequenceiVSP_022828424 – 430AANFCDK → MDLIQEP in isoform 4. Curated7
Alternative sequenceiVSP_022829427 – 430FCDK → MALA in isoform 3. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ507278 mRNA Translation: ABG24573.1
AE014297 Genomic DNA Translation: AAF55007.3
AE014297 Genomic DNA Translation: AAF55008.2
AE014297 Genomic DNA Translation: AAN14346.1
AE014297 Genomic DNA Translation: AAN14347.2
AE014297 Genomic DNA Translation: AAN14348.1
AY121682 mRNA Translation: AAM52009.1
BT012443 mRNA Translation: AAS93714.1
BT021321 mRNA Translation: AAX33469.1
RefSeqiNP_650325.1, NM_142068.2 [Q9VFP2-3]
NP_650326.3, NM_142069.4 [Q9VFP2-1]
NP_731875.2, NM_169564.2 [Q9VFP2-4]
NP_731876.2, NM_169565.2 [Q9VFP2-4]
NP_731877.1, NM_169566.2 [Q9VFP2-2]
UniGeneiDm.10784

Genome annotation databases

EnsemblMetazoaiFBtr0100361; FBpp0099767; FBgn0264493 [Q9VFP2-1]
FBtr0301632; FBpp0290846; FBgn0264493 [Q9VFP2-4]
FBtr0301633; FBpp0290847; FBgn0264493 [Q9VFP2-2]
FBtr0301634; FBpp0290848; FBgn0264493 [Q9VFP2-3]
FBtr0334298; FBpp0306413; FBgn0264493 [Q9VFP2-4]
GeneIDi41704
KEGGidme:Dmel_CG12537

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRDX_DROME
AccessioniPrimary (citable) accession number: Q9VFP2
Secondary accession number(s): A4V2V6
, Q15BT8, Q5BIA3, Q7KSK6, Q8ING4, Q8MRB4, Q9VFP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 7, 2006
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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