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Q9VFI9

- PDE6_DROME

UniProt

Q9VFI9 - PDE6_DROME

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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene
Pde6, CG8279
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has a role regulating cGMP transport in Malpighian tubule principal cells.1 Publication

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.2 Publications

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei718 – 7181Proton donor By similarity
Metal bindingi722 – 7221Divalent metal cation 1 By similarity
Metal bindingi758 – 7581Divalent metal cation 1 By similarity
Metal bindingi759 – 7591Divalent metal cation 1 By similarity
Metal bindingi759 – 7591Divalent metal cation 2 By similarity
Metal bindingi869 – 8691Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. cGMP metabolic process Source: UniProtKB
  2. negative regulation of nucleobase-containing compound transport Source: FlyBase
  3. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
Alternative name(s):
DmPDE5/6
Short name:
DmPDE6
Gene namesi
Name:Pde6
ORF Names:CG8279
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038237. Pde6.

Subcellular locationi

Cell membrane; Lipid-anchor; Cytoplasmic side
Note: Expressed on the apical side of the Malpighian tubule principal cell polarized membrane. Loss of prenylation causes protein location to the cytoplasm.2 Publications

GO - Cellular componenti

  1. apical plasma membrane Source: FlyBase
  2. membrane Source: UniProtKB
  3. plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Flies display increased active transport of cGMP. Overexpression of Pde6 confers inhibition of the active transport and efflux of cGMP by tubules.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1115 – 11151C → S: Impairs prenylation and membrane. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11151115cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000356892Add
BLAST
Propeptidei1116 – 11183Removed in mature form InferredPRO_0000356893

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1115 – 11151Cysteine methyl ester Inferred
Lipidationi1115 – 11151S-farnesyl cysteine Inferred

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiQ9VFI9.

Expressioni

Tissue specificityi

Expressed in Malpighian tubule principal cells.1 Publication

Gene expression databases

BgeeiQ9VFI9.

Interactioni

Subunit structurei

Interacts with PrBP.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9VFI9.
SMRiQ9VFI9. Positions 230-964.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini247 – 399153GAF 1Add
BLAST
Domaini431 – 612182GAF 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 3329Ser-richAdd
BLAST

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
GeneTreeiENSGT00750000117253.
InParanoidiQ9VFI9.
KOiK13763.
OrthoDBiEOG7RRF69.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VFI9-1 [UniParc]FASTAAdd to Basket

« Hide

MTDVSSPAGG AASPVEMSTT SSSSAATTSA SSSKPLTNGA NKTAISTAAG     50
GVTPGAVPGP GSGAIPASSS SGNQVKLEHH HRQSNNNRPA VTNRSSETKL 100
MTPTGSSSSP SQSPSQTQAS IQTQTSQQDR LAKASTTASQ QDVDEVARLF 150
EEKPEAFEKW LTERAPPEAL SRLQEFIENR KPHKRPSVTS DLFQQWMAAS 200
PTVQQKSPRS LSNSSASSLP ECRRHLMDLD EGELFMELIR DVANELDIDV 250
LCHKILVNVG LLTHADRGSL FLAKGTPTNK YLVAKLFDVT QKTALKDAVT 300
RASAEEIIIP FGIGIAGMVA QTKQMINIKE AYKDARFNCE IDLKTGYKTN 350
AILCMPICNY EGDIIGVAQI INKTNGCMEF DEHDVEIFRR YLTFCGIGIQ 400
NAQLFEMSVQ EYRRNQILLN LARSIFEEQN NLECLVTKIM TEARELLKCE 450
RCSVFLVDLD CCEASHLEKI IEKPNQPATR AIKSADSFEE KKMRNRFTVL 500
FELGGEYQAA NVSRPSVSEL SSSTLAQIAQ FVATTGQTVN ICDVIEWVRD 550
HNQIRAEDEI DSTQAILCMP IMNAQKKVIG VAQLINKANG VPFTDSDASI 600
FEAFAIFCGL GIHNTQMYEN ACKLMAKQKV ALECLSYHAT ASQDQTEKLT 650
QDVIAEAESY NLYSFTFTDF ELVDDDTCRA VLRMFMQCNL VSQFQIPYDV 700
LCRWVLSVRK NYRPVKYHNW RHALNVAQTM FAMLKTGKME RFMTDLEILG 750
LLVACLCHDL DHRGTNNAFQ TKTESPLAIL YTTSTMEHHH FDQCVMILNS 800
EGNNIFQALS PEDYRSVMKT VESAILSTDL AMYFKKRNAF LELVENGEFD 850
WQGEEKKDLL CGMMMTACDV SAIAKPWEVQ HKVAKLVADE FFDQGDLEKL 900
QLNTQPVAMM DRERKDELPK MQVGFIDVIC LPLYRVLCDT FPWITPLYEG 950
TLENRRNWQD LAEKVEMGLT WIDHDTIDKP VEEFAACADE EIKDIEFTVT 1000
TLNCNQQSQH GSEDSHTPEH QRSGSRLSMK KTGALGKAVR SKLSKTLYNS 1050
MDGSKPKTSL KLLESHVSED MDDKSPTSPS QPQASGSMGR MSASSSTSSA 1100
GGQMVDKSKK RSKLCALL 1118
Length:1,118
Mass (Da):124,325
Last modified:October 19, 2011 - v4
Checksum:i1CFB89721F84DC5A
GO

Sequence cautioni

The sequence AAL13699.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF55066.4.
AY058470 mRNA. Translation: AAL13699.1. Different initiation.
RefSeqiNP_650369.3. NM_142112.4.
UniGeneiDm.4259.

Genome annotation databases

EnsemblMetazoaiFBtr0300330; FBpp0289559; FBgn0038237.
GeneIDi41760.
KEGGidme:Dmel_CG8279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF55066.4 .
AY058470 mRNA. Translation: AAL13699.1 . Different initiation.
RefSeqi NP_650369.3. NM_142112.4.
UniGenei Dm.4259.

3D structure databases

ProteinModelPortali Q9VFI9.
SMRi Q9VFI9. Positions 230-964.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q9VFI9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0300330 ; FBpp0289559 ; FBgn0038237 .
GeneIDi 41760.
KEGGi dme:Dmel_CG8279.

Organism-specific databases

CTDi 41760.
FlyBasei FBgn0038237. Pde6.

Phylogenomic databases

eggNOGi NOG270709.
GeneTreei ENSGT00750000117253.
InParanoidi Q9VFI9.
KOi K13763.
OrthoDBi EOG7RRF69.

Miscellaneous databases

ChiTaRSi Pde6. drosophila.
GenomeRNAii 41760.
NextBioi 825438.
PROi Q9VFI9.

Gene expression databases

Bgeei Q9VFI9.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 84-1118.
    Strain: Berkeley.
    Tissue: Head.
  4. "A novel role for a Drosophila homologue of cGMP-specific phosphodiesterase in the active transport of cGMP."
    Day J.P., Houslay M.D., Davies S.-A.
    Biochem. J. 393:481-488(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.
  5. "Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein."
    Day J.P., Cleghon V., Houslay M.D., Davies S.-A.
    Biochem. J. 414:363-374(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, INTERACTION WITH PRBP, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-1115.

Entry informationi

Entry nameiPDE6_DROME
AccessioniPrimary (citable) accession number: Q9VFI9
Secondary accession number(s): Q95TW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: October 19, 2011
Last modified: July 9, 2014
This is version 97 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi