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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the second messager cGMP, which is a key regulator of many important physiological processes (PubMed:15673286). Has cAMP phosphodiesterase activity in vitro but not in vivo (PubMed:15673286). Has a role regulating cGMP transport in Malpighian tubule principal cells (PubMed:16232123).2 Publications

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.3 Publications

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by sildenafil and zaprinast.1 Publication

Kineticsi

  1. KM=37 µM for cGMP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei718Proton donorBy similarity1
    Metal bindingi722Divalent metal cation 1By similarity1
    Metal bindingi758Divalent metal cation 1By similarity1
    Metal bindingi759Divalent metal cation 1By similarity1
    Metal bindingi759Divalent metal cation 2By similarity1
    Metal bindingi869Divalent metal cation 1By similarity1

    GO - Molecular functioni

    • 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    • cGMP metabolic process Source: UniProtKB
    • negative regulation of nucleobase-containing compound transport Source: FlyBase
    • signal transduction Source: InterPro

    Keywordsi

    Molecular functionHydrolase
    LigandcGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiR-DME-2485179. Activation of the phototransduction cascade.
    R-DME-4086398. Ca2+ pathway.
    R-DME-418457. cGMP effects.
    R-DME-418555. G alpha (s) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase1 Publication (EC:3.1.4.353 Publications)
    Alternative name(s):
    DmPDE5/6
    Short name:
    DmPDE61 Publication
    Gene namesi
    Name:Pde6
    ORF Names:CG8279
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    Proteomesi
    • UP000000803 Componenti: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0038237. Pde6.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Flies display increased active transport of cGMP. Overexpression of Pde6 confers inhibition of the active transport and efflux of cGMP by tubules.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi1115C → S: Impairs prenylation and membrane. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003568921 – 1115cGMP-specific 3',5'-cyclic phosphodiesteraseAdd BLAST1115
    PropeptideiPRO_00003568931116 – 1118Removed in mature formCurated3

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1115Cysteine methyl esterCurated1
    Lipidationi1115S-farnesyl cysteine1 Publication1

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PaxDbiQ9VFI9.
    PRIDEiQ9VFI9.

    Expressioni

    Tissue specificityi

    Expressed in Malpighian tubule principal cells (PubMed:16232123, PubMed:15673286). Also expressed in adult head (PubMed:15673286).2 Publications

    Gene expression databases

    BgeeiFBgn0038237.
    ExpressionAtlasiQ9VFI9. differential.
    GenevisibleiQ9VFI9. DM.

    Interactioni

    Subunit structurei

    Interacts with PrBP.1 Publication

    Protein-protein interaction databases

    BioGridi66835. 1 interactor.
    STRINGi7227.FBpp0303081.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VFI9.
    SMRiQ9VFI9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini247 – 399GAF 1Add BLAST153
    Domaini431 – 612GAF 2Add BLAST182

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi5 – 33Ser-richAdd BLAST29

    Sequence similaritiesi

    Belongs to the cyclic nucleotide phosphodiesterase family.Sequence analysis

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiKOG3689. Eukaryota.
    ENOG410XRI7. LUCA.
    GeneTreeiENSGT00760000119066.
    InParanoidiQ9VFI9.
    KOiK13763.
    OrthoDBiEOG091G04JU.

    Family and domain databases

    CDDicd00077. HDc. 1 hit.
    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProiView protein in InterPro
    IPR003018. GAF.
    IPR029016. GAF-like_dom_sf.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR036971. PDEase_catalytic_dom_sf.
    IPR023174. PDEase_CS.
    PfamiView protein in Pfam
    PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiView protein in SMART
    SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiView protein in PROSITE
    PS00126. PDEASE_I. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9VFI9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDVSSPAGG AASPVEMSTT SSSSAATTSA SSSKPLTNGA NKTAISTAAG
    60 70 80 90 100
    GVTPGAVPGP GSGAIPASSS SGNQVKLEHH HRQSNNNRPA VTNRSSETKL
    110 120 130 140 150
    MTPTGSSSSP SQSPSQTQAS IQTQTSQQDR LAKASTTASQ QDVDEVARLF
    160 170 180 190 200
    EEKPEAFEKW LTERAPPEAL SRLQEFIENR KPHKRPSVTS DLFQQWMAAS
    210 220 230 240 250
    PTVQQKSPRS LSNSSASSLP ECRRHLMDLD EGELFMELIR DVANELDIDV
    260 270 280 290 300
    LCHKILVNVG LLTHADRGSL FLAKGTPTNK YLVAKLFDVT QKTALKDAVT
    310 320 330 340 350
    RASAEEIIIP FGIGIAGMVA QTKQMINIKE AYKDARFNCE IDLKTGYKTN
    360 370 380 390 400
    AILCMPICNY EGDIIGVAQI INKTNGCMEF DEHDVEIFRR YLTFCGIGIQ
    410 420 430 440 450
    NAQLFEMSVQ EYRRNQILLN LARSIFEEQN NLECLVTKIM TEARELLKCE
    460 470 480 490 500
    RCSVFLVDLD CCEASHLEKI IEKPNQPATR AIKSADSFEE KKMRNRFTVL
    510 520 530 540 550
    FELGGEYQAA NVSRPSVSEL SSSTLAQIAQ FVATTGQTVN ICDVIEWVRD
    560 570 580 590 600
    HNQIRAEDEI DSTQAILCMP IMNAQKKVIG VAQLINKANG VPFTDSDASI
    610 620 630 640 650
    FEAFAIFCGL GIHNTQMYEN ACKLMAKQKV ALECLSYHAT ASQDQTEKLT
    660 670 680 690 700
    QDVIAEAESY NLYSFTFTDF ELVDDDTCRA VLRMFMQCNL VSQFQIPYDV
    710 720 730 740 750
    LCRWVLSVRK NYRPVKYHNW RHALNVAQTM FAMLKTGKME RFMTDLEILG
    760 770 780 790 800
    LLVACLCHDL DHRGTNNAFQ TKTESPLAIL YTTSTMEHHH FDQCVMILNS
    810 820 830 840 850
    EGNNIFQALS PEDYRSVMKT VESAILSTDL AMYFKKRNAF LELVENGEFD
    860 870 880 890 900
    WQGEEKKDLL CGMMMTACDV SAIAKPWEVQ HKVAKLVADE FFDQGDLEKL
    910 920 930 940 950
    QLNTQPVAMM DRERKDELPK MQVGFIDVIC LPLYRVLCDT FPWITPLYEG
    960 970 980 990 1000
    TLENRRNWQD LAEKVEMGLT WIDHDTIDKP VEEFAACADE EIKDIEFTVT
    1010 1020 1030 1040 1050
    TLNCNQQSQH GSEDSHTPEH QRSGSRLSMK KTGALGKAVR SKLSKTLYNS
    1060 1070 1080 1090 1100
    MDGSKPKTSL KLLESHVSED MDDKSPTSPS QPQASGSMGR MSASSSTSSA
    1110
    GGQMVDKSKK RSKLCALL
    Length:1,118
    Mass (Da):124,325
    Last modified:October 19, 2011 - v4
    Checksum:i1CFB89721F84DC5A
    GO

    Sequence cautioni

    The sequence AAL13699 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE014297 Genomic DNA. Translation: AAF55066.4.
    AY058470 mRNA. Translation: AAL13699.1. Different initiation.
    RefSeqiNP_001287327.1. NM_001300398.1.
    NP_650369.3. NM_142112.5.

    Genome annotation databases

    EnsemblMetazoaiFBtr0300330; FBpp0289559; FBgn0038237.
    FBtr0345168; FBpp0311378; FBgn0038237.
    GeneIDi41760.
    KEGGidme:Dmel_CG8279.

    Similar proteinsi

    Entry informationi

    Entry nameiPDE6_DROME
    AccessioniPrimary (citable) accession number: Q9VFI9
    Secondary accession number(s): Q95TW8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: October 19, 2011
    Last modified: November 22, 2017
    This is version 124 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families