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Q9VFC8 (GYS_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogen [starch] synthase

EC=2.4.1.11
Alternative name(s):
Glycogen synthase
Gene names
Name:GlyS
ORF Names:CG6904
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length709 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers the glycosyl residue from UDPG to the non-reducing end of alpha-1,4-glucan. In larval skeletal muscle, isoform B is required for the formation of autophagosomes during starvation and during cloroquine-induced vacuolar myopathy. Ref.6

Catalytic activity

UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Subunit structure

Isoform B interacts with Atg8a upon starvation. Ref.6

Subcellular location

Cytoplasmic vesicleautophagosome. Note: Isoform B colocalizes with Atg8a at autophagosomes upon starvation in larval skeletal muscle. Ref.6

Tissue specificity

In third instar larvae, isoform B is highly expressed in skeletal muscle but not detected in fat body. Ref.6

Sequence similarities

Belongs to the glycosyltransferase 3 family.

Ontologies

Keywords
   Biological processGlycogen biosynthesis
   Cellular componentCytoplasmic vesicle
   Coding sequence diversityAlternative splicing
   Molecular functionGlycosyltransferase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycogen biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentautophagic vacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionglycogen (starch) synthase activity

Inferred from direct assay PubMed 2121604. Source: FlyBase

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9VFC8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: Q9VFC8-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 709709Glycogen [starch] synthase
PRO_0000194771

Sites

Binding site611UDP-glucose By similarity

Amino acid modifications

Modified residue261Phosphoserine Ref.5
Modified residue301Phosphoserine Ref.4 Ref.5
Modified residue6601Phosphoserine Ref.4 Ref.5
Modified residue6671Phosphoserine Ref.5
Modified residue6711Phosphoserine Ref.5
Modified residue6751Phosphoserine Ref.5
Modified residue6791Phosphoserine Ref.5
Modified residue6821Phosphothreonine Ref.5
Modified residue6831Phosphothreonine Ref.5
Modified residue6871Phosphoserine Ref.5
Modified residue6911Phosphoserine Ref.5
Modified residue6961Phosphoserine Ref.5

Natural variations

Alternative sequence1 – 2020Missing in isoform B.
VSP_010302

Experimental info

Mutagenesis6131R → A: Abolishes interaction with Atg8a. Ref.6
Mutagenesis6291W → A: Abolishes interaction with Atg8a. Ref.6
Mutagenesis6711S → A: Does not affect interaction with Atg8a. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified May 10, 2004. Version 2.
Checksum: 23E6381BC900CE54

FASTA70981,754
        10         20         30         40         50         60 
MRRQQSYRFE DNESTSYALR MNRRFSRVES GADLKDYFDR GDIASRENRW NFEVAWEVAN 

        70         80         90        100        110        120 
KVGGIYTVIR SKAYVSTEEM GEQLCMMGPY KEHCARTEME EMEFPRGNPL LDAVNSLRSR 

       130        140        150        160        170        180 
GYKIHTGRWL VDGNPQLILF DIGSAAWKLD QFKSEMWEKC HIGIPHLDIE TNDAIILGFM 

       190        200        210        220        230        240 
IAEFLEEFRN FAVTYSQNNE LSAPRIVAHF HEWQAGVGLI VLRTRLVEIA TVFTTHATLL 

       250        260        270        280        290        300 
GRYLCAGNTD FYNNLDKFAV DEEAGKRQIY HRYCLERGAT HLAHVFTTVS EITGYEAEHL 

       310        320        330        340        350        360 
LKRKPDIITP NGLNVKKFSA IHEFQNLHAV AKEKINEFVR GHFYGHIDFD LDKTLYFFIA 

       370        380        390        400        410        420 
GRYEFGNKGA DIFIEALARL NAMLKHEKPD TTVVAFLIFP TKTNNFNVDS LRGHAVIKQL 

       430        440        450        460        470        480 
RDTINNVQQA VGKRMFDTCL QGNIPNADDL LQKDDLVKIK RCMFAMQRDS MPPVTTHNVA 

       490        500        510        520        530        540 
DDWNDPVLSS IRRCHLFNSR HDRVKMVFHP EFLTSTNPLF GIDYEEFVRG CHLGVFPSYY 

       550        560        570        580        590        600 
EPWGYTPAEC TVMGIPSVTT NLSGFGCFME EHISDPKSYG IYIVDRRYIG LENSVQQLSS 

       610        620        630        640        650        660 
FMMEFSRLNR RQRIIQRNRT ERLSDLLDWR TLGIYYRQAR VKALQAVYPD YVDELSLYGS 

       670        680        690        700 
KNNLIFSRPH SEPPSPTSSR HTTPAPSVHG SDDEDSVDEE TELKELGIK 

« Hide

Isoform B (C) [UniParc].

Checksum: F29348548680BB2A
Show »

FASTA68979,234

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Embryo.
[4]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-660, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-30; SER-660; SER-667; SER-671; SER-675; SER-679; THR-682; THR-683; SER-687; SER-691 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
[6]"Role of autophagy in glycogen breakdown and its relevance to chloroquine myopathy."
Zirin J., Nieuwenhuis J., Perrimon N.
PLoS Biol. 11:E1001708-E1001708(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ATG8A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-613; TRP-629 AND SER-671.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014297 Genomic DNA. Translation: AAF55132.2.
AE014297 Genomic DNA. Translation: AAN13623.1.
AE014297 Genomic DNA. Translation: AAN13624.1.
AY052005 mRNA. Translation: AAK93429.1.
RefSeqNP_001262583.1. NM_001275654.1. [Q9VFC8-2]
NP_650422.1. NM_142165.3. [Q9VFC8-2]
NP_731967.2. NM_169610.2. [Q9VFC8-1]
NP_731968.1. NM_169611.2. [Q9VFC8-2]
UniGeneDm.4414.

3D structure databases

ProteinModelPortalQ9VFC8.
SMRQ9VFC8. Positions 47-655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid66888. 14 interactions.
DIPDIP-19447N.
IntActQ9VFC8. 5 interactions.
MINTMINT-332090.

Protein family/group databases

CAZyGT3. Glycosyltransferase Family 3.

Proteomic databases

PaxDbQ9VFC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0083037; FBpp0082496; FBgn0038293. [Q9VFC8-1]
GeneID41823.
KEGGdme:Dmel_CG6904.

Organism-specific databases

FlyBaseFBgn0266064. GlyS.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000018612.
InParanoidQ9VFC8.
KOK00693.
OMAEVSWEVT.
OrthoDBEOG741Z1N.
PhylomeDBQ9VFC8.

Enzyme and pathway databases

UniPathwayUPA00164.

Gene expression databases

BgeeQ9VFC8.

Family and domain databases

InterProIPR008631. Glycogen_synth.
[Graphical view]
PANTHERPTHR10176. PTHR10176. 1 hit.
PfamPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi41823.
NextBio825719.

Entry information

Entry nameGYS_DROME
AccessionPrimary (citable) accession number: Q9VFC8
Secondary accession number(s): A4V2X5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 10, 2004
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase