Reviewed, UniProtKB/Swiss-Prot Q9VFC8 (GYS_DROME)

Names and origin

Protein names

Recommended name:
    Putative glycogen [starch] synthase
    EC=2.4.1.11

Gene names

ORF Names:

CG6904

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers the glycosyl residue from UDPG to the non-reducing end of alpha-1,4-glucan.
Catalytic activity	UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).
Pathway	Glycan biosynthesis; glycogen biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 3 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycogen (starch) synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VMJ2	1	EBI-169205,EBI-132767
	Q9VX51	1	EBI-169205,EBI-92858
	Q9W392	1	EBI-169205,EBI-192297
beat-Ia	Q94534	1	EBI-169205,EBI-113385
Tk	Q9VGE8	1	EBI-169205,EBI-152254

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VFC8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q9VFC8-2)

Also known as: C;

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Putative glycogen [starch] synthase		PRO_0000194771
Sites
	Binding site	61	1	UDP-glucose By similarity
Amino acid modifications
	Modified residue	26	1	Phosphoserine Ref.5
	Modified residue	30	1	Phosphoserine Ref.5 Ref.4
	Modified residue	660	1	Phosphoserine Ref.5 Ref.4
	Modified residue	667	1	Phosphoserine Ref.5
	Modified residue	671	1	Phosphoserine Ref.5
	Modified residue	675	1	Phosphoserine Ref.5
	Modified residue	679	1	Phosphoserine Ref.5
	Modified residue	682	1	Phosphothreonine Ref.5
	Modified residue	683	1	Phosphothreonine Ref.5
	Modified residue	687	1	Phosphoserine Ref.5
	Modified residue	691	1	Phosphoserine Ref.5
	Modified residue	696	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 20	20	Missing in isoform B.		VSP_010302

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 23E6381BC900CE54	FASTA	709	81,754
	10 20 30 40 50 60 MRRQQSYRFE DNESTSYALR MNRRFSRVES GADLKDYFDR GDIASRENRW NFEVAWEVAN 70 80 90 100 110 120 KVGGIYTVIR SKAYVSTEEM GEQLCMMGPY KEHCARTEME EMEFPRGNPL LDAVNSLRSR 130 140 150 160 170 180 GYKIHTGRWL VDGNPQLILF DIGSAAWKLD QFKSEMWEKC HIGIPHLDIE TNDAIILGFM 190 200 210 220 230 240 IAEFLEEFRN FAVTYSQNNE LSAPRIVAHF HEWQAGVGLI VLRTRLVEIA TVFTTHATLL 250 260 270 280 290 300 GRYLCAGNTD FYNNLDKFAV DEEAGKRQIY HRYCLERGAT HLAHVFTTVS EITGYEAEHL 310 320 330 340 350 360 LKRKPDIITP NGLNVKKFSA IHEFQNLHAV AKEKINEFVR GHFYGHIDFD LDKTLYFFIA 370 380 390 400 410 420 GRYEFGNKGA DIFIEALARL NAMLKHEKPD TTVVAFLIFP TKTNNFNVDS LRGHAVIKQL 430 440 450 460 470 480 RDTINNVQQA VGKRMFDTCL QGNIPNADDL LQKDDLVKIK RCMFAMQRDS MPPVTTHNVA 490 500 510 520 530 540 DDWNDPVLSS IRRCHLFNSR HDRVKMVFHP EFLTSTNPLF GIDYEEFVRG CHLGVFPSYY 550 560 570 580 590 600 EPWGYTPAEC TVMGIPSVTT NLSGFGCFME EHISDPKSYG IYIVDRRYIG LENSVQQLSS 610 620 630 640 650 660 FMMEFSRLNR RQRIIQRNRT ERLSDLLDWR TLGIYYRQAR VKALQAVYPD YVDELSLYGS 670 680 690 700 KNNLIFSRPH SEPPSPTSSR HTTPAPSVHG SDDEDSVDEE TELKELGIK
	Isoform B (C). Checksum: F29348548680BB2A	FASTA	689	79,234
	10 20 30 40 50 60 MNRRFSRVES GADLKDYFDR GDIASRENRW NFEVAWEVAN KVGGIYTVIR SKAYVSTEEM 70 80 90 100 110 120 GEQLCMMGPY KEHCARTEME EMEFPRGNPL LDAVNSLRSR GYKIHTGRWL VDGNPQLILF 130 140 150 160 170 180 DIGSAAWKLD QFKSEMWEKC HIGIPHLDIE TNDAIILGFM IAEFLEEFRN FAVTYSQNNE 190 200 210 220 230 240 LSAPRIVAHF HEWQAGVGLI VLRTRLVEIA TVFTTHATLL GRYLCAGNTD FYNNLDKFAV 250 260 270 280 290 300 DEEAGKRQIY HRYCLERGAT HLAHVFTTVS EITGYEAEHL LKRKPDIITP NGLNVKKFSA 310 320 330 340 350 360 IHEFQNLHAV AKEKINEFVR GHFYGHIDFD LDKTLYFFIA GRYEFGNKGA DIFIEALARL 370 380 390 400 410 420 NAMLKHEKPD TTVVAFLIFP TKTNNFNVDS LRGHAVIKQL RDTINNVQQA VGKRMFDTCL 430 440 450 460 470 480 QGNIPNADDL LQKDDLVKIK RCMFAMQRDS MPPVTTHNVA DDWNDPVLSS IRRCHLFNSR 490 500 510 520 530 540 HDRVKMVFHP EFLTSTNPLF GIDYEEFVRG CHLGVFPSYY EPWGYTPAEC TVMGIPSVTT 550 560 570 580 590 600 NLSGFGCFME EHISDPKSYG IYIVDRRYIG LENSVQQLSS FMMEFSRLNR RQRIIQRNRT 610 620 630 640 650 660 ERLSDLLDWR TLGIYYRQAR VKALQAVYPD YVDELSLYGS KNNLIFSRPH SEPPSPTSSR 670 680 HTTPAPSVHG SDDEDSVDEE TELKELGIK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Embryo.
[4]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-660, MASS SPECTROMETRY.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-30; SER-660; SER-667; SER-671; SER-675; SER-679; THR-682; THR-683; SER-687; SER-691 AND SER-696, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAF55132.2. AE014297 Genomic DNA. Translation: AAN13623.1. AE014297 Genomic DNA. Translation: AAN13624.1. AY052005 mRNA. Translation: AAK93429.1.
RefSeq	NP_650422.1. NP_731967.2. NP_731968.1.
UniGene	Dm.4414
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19447N.
IntAct	Q9VFC8. 13 interactions.
Protein family/group databases
CAZy	GT3. Glycosyltransferase Family 3.
Genome annotation databases
Ensembl	FBgn0038293. Drosophila melanogaster. [Contig view]
GeneID	41823.
KEGG	dme:Dmel_CG6904.
Organism-specific databases
FlyBase	FBgn0038293. CG6904.
Phylogenomic databases
HOGENOM	Q9VFC8.
OMA	Q9VFC8. FTTVSHI.
Enzyme and pathway databases
BRENDA	2.4.1.11. 48.
Gene expression databases
ArrayExpress	Q9VFC8.
GermOnline	CG6904. Drosophila melanogaster.
Family and domain databases
InterPro	IPR008631. Glycogen_synth. [Graphical view]
PANTHER	PTHR10176. Glycogen_synth. 1 hit.
Pfam	PF05693. Glycogen_syn. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	825719.

Entry information

Entry name

GYS_DROME

Accession

Primary (citable) accession number: Q9VFC8
Secondary accession number(s): A4V2X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project