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Q9VFC8

- GYS_DROME

UniProt

Q9VFC8 - GYS_DROME

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Protein

Glycogen [starch] synthase

Gene

GlyS

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transfers the glycosyl residue from UDPG to the non-reducing end of alpha-1,4-glucan. In larval skeletal muscle, isoform B is required for the formation of autophagosomes during starvation and during cloroquine-induced vacuolar myopathy.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611UDP-glucoseBy similarity

GO - Molecular functioni

  1. glycogen (starch) synthase activity Source: FlyBase

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase (EC:2.4.1.11)
Alternative name(s):
Glycogen synthase
Gene namesi
Name:GlyS
ORF Names:CG6904
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266064. GlyS.

Subcellular locationi

Cytoplasmic vesicleautophagosome 1 Publication
Note: Isoform B colocalizes with Atg8a at autophagosomes upon starvation in larval skeletal muscle.

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi613 – 6131R → A: Abolishes interaction with Atg8a. 1 Publication
Mutagenesisi629 – 6291W → A: Abolishes interaction with Atg8a. 1 Publication
Mutagenesisi671 – 6711S → A: Does not affect interaction with Atg8a. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Glycogen [starch] synthasePRO_0000194771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261Phosphoserine1 Publication
Modified residuei30 – 301Phosphoserine2 Publications
Modified residuei660 – 6601Phosphoserine2 Publications
Modified residuei667 – 6671Phosphoserine1 Publication
Modified residuei671 – 6711Phosphoserine1 Publication
Modified residuei675 – 6751Phosphoserine1 Publication
Modified residuei679 – 6791Phosphoserine1 Publication
Modified residuei682 – 6821Phosphothreonine1 Publication
Modified residuei683 – 6831Phosphothreonine1 Publication
Modified residuei687 – 6871Phosphoserine1 Publication
Modified residuei691 – 6911Phosphoserine1 Publication
Modified residuei696 – 6961Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VFC8.

Expressioni

Tissue specificityi

In third instar larvae, isoform B is highly expressed in skeletal muscle but not detected in fat body.1 Publication

Gene expression databases

BgeeiQ9VFC8.
ExpressionAtlasiQ9VFC8. differential.

Interactioni

Subunit structurei

Isoform B interacts with Atg8a upon starvation.1 Publication

Protein-protein interaction databases

BioGridi66888. 14 interactions.
DIPiDIP-19447N.
IntActiQ9VFC8. 5 interactions.
MINTiMINT-332090.

Structurei

3D structure databases

ProteinModelPortaliQ9VFC8.
SMRiQ9VFC8. Positions 47-655.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 3 family.Curated

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
InParanoidiQ9VFC8.
KOiK00693.
OMAiEVSWEVT.
OrthoDBiEOG741Z1N.
PhylomeDBiQ9VFC8.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q9VFC8-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRQQSYRFE DNESTSYALR MNRRFSRVES GADLKDYFDR GDIASRENRW
60 70 80 90 100
NFEVAWEVAN KVGGIYTVIR SKAYVSTEEM GEQLCMMGPY KEHCARTEME
110 120 130 140 150
EMEFPRGNPL LDAVNSLRSR GYKIHTGRWL VDGNPQLILF DIGSAAWKLD
160 170 180 190 200
QFKSEMWEKC HIGIPHLDIE TNDAIILGFM IAEFLEEFRN FAVTYSQNNE
210 220 230 240 250
LSAPRIVAHF HEWQAGVGLI VLRTRLVEIA TVFTTHATLL GRYLCAGNTD
260 270 280 290 300
FYNNLDKFAV DEEAGKRQIY HRYCLERGAT HLAHVFTTVS EITGYEAEHL
310 320 330 340 350
LKRKPDIITP NGLNVKKFSA IHEFQNLHAV AKEKINEFVR GHFYGHIDFD
360 370 380 390 400
LDKTLYFFIA GRYEFGNKGA DIFIEALARL NAMLKHEKPD TTVVAFLIFP
410 420 430 440 450
TKTNNFNVDS LRGHAVIKQL RDTINNVQQA VGKRMFDTCL QGNIPNADDL
460 470 480 490 500
LQKDDLVKIK RCMFAMQRDS MPPVTTHNVA DDWNDPVLSS IRRCHLFNSR
510 520 530 540 550
HDRVKMVFHP EFLTSTNPLF GIDYEEFVRG CHLGVFPSYY EPWGYTPAEC
560 570 580 590 600
TVMGIPSVTT NLSGFGCFME EHISDPKSYG IYIVDRRYIG LENSVQQLSS
610 620 630 640 650
FMMEFSRLNR RQRIIQRNRT ERLSDLLDWR TLGIYYRQAR VKALQAVYPD
660 670 680 690 700
YVDELSLYGS KNNLIFSRPH SEPPSPTSSR HTTPAPSVHG SDDEDSVDEE

TELKELGIK

Note: No experimental confirmation available.

Length:709
Mass (Da):81,754
Last modified:May 10, 2004 - v2
Checksum:i23E6381BC900CE54
GO
Isoform B (identifier: Q9VFC8-2) [UniParc]FASTAAdd to Basket

Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: No experimental confirmation available.

Show »
Length:689
Mass (Da):79,234
Checksum:iF29348548680BB2A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform B. 1 PublicationVSP_010302Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF55132.2.
AE014297 Genomic DNA. Translation: AAN13623.1.
AE014297 Genomic DNA. Translation: AAN13624.1.
AY052005 mRNA. Translation: AAK93429.1.
RefSeqiNP_001262583.1. NM_001275654.1. [Q9VFC8-2]
NP_650422.1. NM_142165.3. [Q9VFC8-2]
NP_731967.2. NM_169610.2. [Q9VFC8-1]
NP_731968.1. NM_169611.2. [Q9VFC8-2]
UniGeneiDm.4414.

Genome annotation databases

EnsemblMetazoaiFBtr0083037; FBpp0082496; FBgn0038293. [Q9VFC8-1]
GeneIDi41823.
KEGGidme:Dmel_CG6904.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014297 Genomic DNA. Translation: AAF55132.2 .
AE014297 Genomic DNA. Translation: AAN13623.1 .
AE014297 Genomic DNA. Translation: AAN13624.1 .
AY052005 mRNA. Translation: AAK93429.1 .
RefSeqi NP_001262583.1. NM_001275654.1. [Q9VFC8-2 ]
NP_650422.1. NM_142165.3. [Q9VFC8-2 ]
NP_731967.2. NM_169610.2. [Q9VFC8-1 ]
NP_731968.1. NM_169611.2. [Q9VFC8-2 ]
UniGenei Dm.4414.

3D structure databases

ProteinModelPortali Q9VFC8.
SMRi Q9VFC8. Positions 47-655.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66888. 14 interactions.
DIPi DIP-19447N.
IntActi Q9VFC8. 5 interactions.
MINTi MINT-332090.

Protein family/group databases

CAZyi GT3. Glycosyltransferase Family 3.

Proteomic databases

PaxDbi Q9VFC8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0083037 ; FBpp0082496 ; FBgn0038293 . [Q9VFC8-1 ]
GeneIDi 41823.
KEGGi dme:Dmel_CG6904.

Organism-specific databases

CTDi 41823.
FlyBasei FBgn0266064. GlyS.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00390000018612.
InParanoidi Q9VFC8.
KOi K00693.
OMAi EVSWEVT.
OrthoDBi EOG741Z1N.
PhylomeDBi Q9VFC8.

Enzyme and pathway databases

UniPathwayi UPA00164 .

Miscellaneous databases

GenomeRNAii 41823.
NextBioi 825719.

Gene expression databases

Bgeei Q9VFC8.
ExpressionAtlasi Q9VFC8. differential.

Family and domain databases

InterProi IPR008631. Glycogen_synth.
[Graphical view ]
PANTHERi PTHR10176. PTHR10176. 1 hit.
Pfami PF05693. Glycogen_syn. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Embryo.
  4. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
    Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
    Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-660, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-30; SER-660; SER-667; SER-671; SER-675; SER-679; THR-682; THR-683; SER-687; SER-691 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.
  6. "Role of autophagy in glycogen breakdown and its relevance to chloroquine myopathy."
    Zirin J., Nieuwenhuis J., Perrimon N.
    PLoS Biol. 11:E1001708-E1001708(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG8A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-613; TRP-629 AND SER-671.

Entry informationi

Entry nameiGYS_DROME
AccessioniPrimary (citable) accession number: Q9VFC8
Secondary accession number(s): A4V2X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 10, 2004
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3