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Q9VFC8

- GYS_DROME

UniProt

Q9VFC8 - GYS_DROME

Protein

Glycogen [starch] synthase

Gene

GlyS

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (10 May 2004)
      Previous versions | rss
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    Functioni

    Transfers the glycosyl residue from UDPG to the non-reducing end of alpha-1,4-glucan. In larval skeletal muscle, isoform B is required for the formation of autophagosomes during starvation and during cloroquine-induced vacuolar myopathy.1 Publication

    Catalytic activityi

    UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611UDP-glucoseBy similarity

    GO - Molecular functioni

    1. glycogen (starch) synthase activity Source: FlyBase

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiGT3. Glycosyltransferase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen [starch] synthase (EC:2.4.1.11)
    Alternative name(s):
    Glycogen synthase
    Gene namesi
    Name:GlyS
    ORF Names:CG6904
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0266064. GlyS.

    Subcellular locationi

    Cytoplasmic vesicleautophagosome 1 Publication
    Note: Isoform B colocalizes with Atg8a at autophagosomes upon starvation in larval skeletal muscle.

    GO - Cellular componenti

    1. autophagic vacuole Source: UniProtKB-SubCell
    2. cytoplasmic vesicle Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi613 – 6131R → A: Abolishes interaction with Atg8a. 1 Publication
    Mutagenesisi629 – 6291W → A: Abolishes interaction with Atg8a. 1 Publication
    Mutagenesisi671 – 6711S → A: Does not affect interaction with Atg8a. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 709709Glycogen [starch] synthasePRO_0000194771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei26 – 261Phosphoserine1 Publication
    Modified residuei30 – 301Phosphoserine2 Publications
    Modified residuei660 – 6601Phosphoserine2 Publications
    Modified residuei667 – 6671Phosphoserine1 Publication
    Modified residuei671 – 6711Phosphoserine1 Publication
    Modified residuei675 – 6751Phosphoserine1 Publication
    Modified residuei679 – 6791Phosphoserine1 Publication
    Modified residuei682 – 6821Phosphothreonine1 Publication
    Modified residuei683 – 6831Phosphothreonine1 Publication
    Modified residuei687 – 6871Phosphoserine1 Publication
    Modified residuei691 – 6911Phosphoserine1 Publication
    Modified residuei696 – 6961Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9VFC8.

    Expressioni

    Tissue specificityi

    In third instar larvae, isoform B is highly expressed in skeletal muscle but not detected in fat body.1 Publication

    Gene expression databases

    BgeeiQ9VFC8.

    Interactioni

    Subunit structurei

    Isoform B interacts with Atg8a upon starvation.1 Publication

    Protein-protein interaction databases

    BioGridi66888. 14 interactions.
    DIPiDIP-19447N.
    IntActiQ9VFC8. 5 interactions.
    MINTiMINT-332090.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VFC8.
    SMRiQ9VFC8. Positions 47-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000018612.
    InParanoidiQ9VFC8.
    KOiK00693.
    OMAiEVSWEVT.
    OrthoDBiEOG741Z1N.
    PhylomeDBiQ9VFC8.

    Family and domain databases

    InterProiIPR008631. Glycogen_synth.
    [Graphical view]
    PANTHERiPTHR10176. PTHR10176. 1 hit.
    PfamiPF05693. Glycogen_syn. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q9VFC8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRRQQSYRFE DNESTSYALR MNRRFSRVES GADLKDYFDR GDIASRENRW    50
    NFEVAWEVAN KVGGIYTVIR SKAYVSTEEM GEQLCMMGPY KEHCARTEME 100
    EMEFPRGNPL LDAVNSLRSR GYKIHTGRWL VDGNPQLILF DIGSAAWKLD 150
    QFKSEMWEKC HIGIPHLDIE TNDAIILGFM IAEFLEEFRN FAVTYSQNNE 200
    LSAPRIVAHF HEWQAGVGLI VLRTRLVEIA TVFTTHATLL GRYLCAGNTD 250
    FYNNLDKFAV DEEAGKRQIY HRYCLERGAT HLAHVFTTVS EITGYEAEHL 300
    LKRKPDIITP NGLNVKKFSA IHEFQNLHAV AKEKINEFVR GHFYGHIDFD 350
    LDKTLYFFIA GRYEFGNKGA DIFIEALARL NAMLKHEKPD TTVVAFLIFP 400
    TKTNNFNVDS LRGHAVIKQL RDTINNVQQA VGKRMFDTCL QGNIPNADDL 450
    LQKDDLVKIK RCMFAMQRDS MPPVTTHNVA DDWNDPVLSS IRRCHLFNSR 500
    HDRVKMVFHP EFLTSTNPLF GIDYEEFVRG CHLGVFPSYY EPWGYTPAEC 550
    TVMGIPSVTT NLSGFGCFME EHISDPKSYG IYIVDRRYIG LENSVQQLSS 600
    FMMEFSRLNR RQRIIQRNRT ERLSDLLDWR TLGIYYRQAR VKALQAVYPD 650
    YVDELSLYGS KNNLIFSRPH SEPPSPTSSR HTTPAPSVHG SDDEDSVDEE 700
    TELKELGIK 709

    Note: No experimental confirmation available.

    Length:709
    Mass (Da):81,754
    Last modified:May 10, 2004 - v2
    Checksum:i23E6381BC900CE54
    GO
    Isoform B (identifier: Q9VFC8-2) [UniParc]FASTAAdd to Basket

    Also known as: C

    The sequence of this isoform differs from the canonical sequence as follows:
         1-20: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:689
    Mass (Da):79,234
    Checksum:iF29348548680BB2A
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2020Missing in isoform B. 1 PublicationVSP_010302Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF55132.2.
    AE014297 Genomic DNA. Translation: AAN13623.1.
    AE014297 Genomic DNA. Translation: AAN13624.1.
    AY052005 mRNA. Translation: AAK93429.1.
    RefSeqiNP_001262583.1. NM_001275654.1. [Q9VFC8-2]
    NP_650422.1. NM_142165.3. [Q9VFC8-2]
    NP_731967.2. NM_169610.2. [Q9VFC8-1]
    NP_731968.1. NM_169611.2. [Q9VFC8-2]
    UniGeneiDm.4414.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083037; FBpp0082496; FBgn0038293. [Q9VFC8-1]
    GeneIDi41823.
    KEGGidme:Dmel_CG6904.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF55132.2 .
    AE014297 Genomic DNA. Translation: AAN13623.1 .
    AE014297 Genomic DNA. Translation: AAN13624.1 .
    AY052005 mRNA. Translation: AAK93429.1 .
    RefSeqi NP_001262583.1. NM_001275654.1. [Q9VFC8-2 ]
    NP_650422.1. NM_142165.3. [Q9VFC8-2 ]
    NP_731967.2. NM_169610.2. [Q9VFC8-1 ]
    NP_731968.1. NM_169611.2. [Q9VFC8-2 ]
    UniGenei Dm.4414.

    3D structure databases

    ProteinModelPortali Q9VFC8.
    SMRi Q9VFC8. Positions 47-655.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66888. 14 interactions.
    DIPi DIP-19447N.
    IntActi Q9VFC8. 5 interactions.
    MINTi MINT-332090.

    Protein family/group databases

    CAZyi GT3. Glycosyltransferase Family 3.

    Proteomic databases

    PaxDbi Q9VFC8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083037 ; FBpp0082496 ; FBgn0038293 . [Q9VFC8-1 ]
    GeneIDi 41823.
    KEGGi dme:Dmel_CG6904.

    Organism-specific databases

    FlyBasei FBgn0266064. GlyS.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000018612.
    InParanoidi Q9VFC8.
    KOi K00693.
    OMAi EVSWEVT.
    OrthoDBi EOG741Z1N.
    PhylomeDBi Q9VFC8.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .

    Miscellaneous databases

    GenomeRNAii 41823.
    NextBioi 825719.

    Gene expression databases

    Bgeei Q9VFC8.

    Family and domain databases

    InterProi IPR008631. Glycogen_synth.
    [Graphical view ]
    PANTHERi PTHR10176. PTHR10176. 1 hit.
    Pfami PF05693. Glycogen_syn. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Embryo.
    4. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30 AND SER-660, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-30; SER-660; SER-667; SER-671; SER-675; SER-679; THR-682; THR-683; SER-687; SER-691 AND SER-696, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.
    6. "Role of autophagy in glycogen breakdown and its relevance to chloroquine myopathy."
      Zirin J., Nieuwenhuis J., Perrimon N.
      PLoS Biol. 11:E1001708-E1001708(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ATG8A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF ARG-613; TRP-629 AND SER-671.

    Entry informationi

    Entry nameiGYS_DROME
    AccessioniPrimary (citable) accession number: Q9VFC8
    Secondary accession number(s): A4V2X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: May 10, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3