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Protein

DNA replication licensing factor REC

Gene

rec

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for meiotic DNA recombination in females. Probably not involved in DNA repair and recombination in somatic cells.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri281 – 30828C4-typeSequence analysisAdd
BLAST
Nucleotide bindingi473 – 4808ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: UniProtKB
  • DNA binding Source: UniProtKB-KW
  • DNA-dependent ATPase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA replication initiation Source: UniProtKB
  • reciprocal meiotic recombination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA recombination, Meiosis

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68689. CDC6 association with the ORC:origin complex.
R-DME-68827. CDT1 association with the CDC6:ORC:origin complex.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor REC
Alternative name(s):
Protein mini chromosome maintenance-related
Protein recombination-defective
Gene namesi
Name:rec
ORF Names:CG31293
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003227. rec.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi455 – 4551S → F in rec3; temperature-sensitive allele. Recombination-defective. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885DNA replication licensing factor RECPRO_0000194127Add
BLAST

Proteomic databases

PaxDbiQ9VF30.

Expressioni

Gene expression databases

BgeeiQ9VF30.
GenevisibleiQ9VF30. DM.

Interactioni

Protein-protein interaction databases

BioGridi72108. 1 interaction.
IntActiQ9VF30. 3 interactions.
MINTiMINT-1544375.
STRINGi7227.FBpp0082647.

Structurei

3D structure databases

ProteinModelPortaliQ9VF30.
SMRiQ9VF30. Positions 348-797.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini430 – 627198MCMAdd
BLAST

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri281 – 30828C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00790000123057.
InParanoidiQ9VF30.
OMAiACANPQR.
OrthoDBiEOG7BW0HW.
PhylomeDBiQ9VF30.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR001208. MCM_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VF30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPTLSRGGA PSRFVRGRGR GGGAYRPYFY FRRNGRVIPA GGNRQPNQGE
60 70 80 90 100
PGAPDAPSVP PATRQPRGWS RTAGKRGRNA HLDCSFLRPE NYAAPEDGLQ
110 120 130 140 150
VQSIAVDNPR AYSGWRLYFL REKYEEGNEL ATRIMAVEAY YQRNPHTFDF
160 170 180 190 200
VMIRDRGFFP LCAAGIKSDA QLKEVWPSLS EDIREHPLRT LGTLSLAMHT
210 220 230 240 250
VVVNHQLDCN DSTTAMVSTP EQYVLPTPRV RKIYARPDDF VPVESIEGIS
260 270 280 290 300
HSRVDTLFSI RGFVSNVGEP SYSLTWQAFR CSRCQMEIAM RQRGTFQPRP
310 320 330 340 350
YQCKRSECVA RDEFVPLRSS PYTRLSIRQI IRVEESSLNL VHDFETSMPA
360 370 380 390 400
EMDVELRHDL VDAVRVGQEV VVTGILKLQE LGDDTTTGDT SNQMQPYLKA
410 420 430 440 450
VSIRDASSIK REFSERDLEA IVMINAEPNS FKLLVQSIAP EVYGHELPKA
460 470 480 490 500
ACLLSLLGGK GAETEAINVL LVGDPGIGKT KILQSCAQIA ERGAHVSGKR
510 520 530 540 550
GAQSAQQLGV TFAGRNKRVL QAGSLMMASG GGHCTLDDVD KLASKQAVLL
560 570 580 590 600
QCLQSEEVNL PLAGAFASFP AQPSVIACAN PQRGQYDEGR YLLQNINISP
610 620 630 640 650
SLLREFHLVY ILLDKPSERD MSLTAHVRAL HAGARKRARI AARYALKPKM
660 670 680 690 700
SDSMCEVSLN VPAAGKDDDT IKTEDDNDSI MQQDYDLDKR LEVLPEEGDL
710 720 730 740 750
DLLPPILIKK FLSYARQELN PVLNEDASNA VLRYFLELKG SCNLDEDVSS
760 770 780 790 800
QIGAGQLLAI IHLSQARARL DLSHVVSPQH VRDVIALLTE SITQTSLKEG
810 820 830 840 850
SSRQGTRGGG GAGGGAGKSA QLRNFLELTK RRSAALGRRI FEFDELKEIG
860 870 880
TRAGILTGFS QLVEMANLGG YLLMKGANMY EVVPD
Length:885
Mass (Da):97,585
Last modified:October 1, 2002 - v2
Checksum:i9CDC8D6CF91C21C6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti118 – 1181Y → H.1 Publication
Natural varianti422 – 4221V → A.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120941 mRNA. Translation: BAC92698.1.
AB120942 mRNA. Translation: BAC92699.1.
AB120943 mRNA. Translation: BAC92700.1.
AB120944 Genomic DNA. Translation: BAC92701.1.
AB120945 Genomic DNA. Translation: BAC92702.1.
AB120946 Genomic DNA. Translation: BAC92703.1.
AB120947 Genomic DNA. Translation: BAC92704.1.
AB120948 Genomic DNA. Translation: BAC92705.1.
AB120949 Genomic DNA. Translation: BAC92706.1.
AB120950 Genomic DNA. Translation: BAC92707.1.
AB120951 mRNA. Translation: BAC92708.1.
AB120952 Genomic DNA. Translation: BAC92709.1.
AB120953 mRNA. Translation: BAC92710.1.
AE014297 Genomic DNA. Translation: AAF55231.2.
RefSeqiNP_001262615.1. NM_001275686.1.
NP_732072.1. NM_169674.2.
UniGeneiDm.31313.

Genome annotation databases

EnsemblMetazoaiFBtr0083193; FBpp0082647; FBgn0003227.
FBtr0334595; FBpp0306662; FBgn0003227.
GeneIDi49241.
KEGGidme:Dmel_CG31293.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB120941 mRNA. Translation: BAC92698.1.
AB120942 mRNA. Translation: BAC92699.1.
AB120943 mRNA. Translation: BAC92700.1.
AB120944 Genomic DNA. Translation: BAC92701.1.
AB120945 Genomic DNA. Translation: BAC92702.1.
AB120946 Genomic DNA. Translation: BAC92703.1.
AB120947 Genomic DNA. Translation: BAC92704.1.
AB120948 Genomic DNA. Translation: BAC92705.1.
AB120949 Genomic DNA. Translation: BAC92706.1.
AB120950 Genomic DNA. Translation: BAC92707.1.
AB120951 mRNA. Translation: BAC92708.1.
AB120952 Genomic DNA. Translation: BAC92709.1.
AB120953 mRNA. Translation: BAC92710.1.
AE014297 Genomic DNA. Translation: AAF55231.2.
RefSeqiNP_001262615.1. NM_001275686.1.
NP_732072.1. NM_169674.2.
UniGeneiDm.31313.

3D structure databases

ProteinModelPortaliQ9VF30.
SMRiQ9VF30. Positions 348-797.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi72108. 1 interaction.
IntActiQ9VF30. 3 interactions.
MINTiMINT-1544375.
STRINGi7227.FBpp0082647.

Proteomic databases

PaxDbiQ9VF30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083193; FBpp0082647; FBgn0003227.
FBtr0334595; FBpp0306662; FBgn0003227.
GeneIDi49241.
KEGGidme:Dmel_CG31293.

Organism-specific databases

CTDi49241.
FlyBaseiFBgn0003227. rec.

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00790000123057.
InParanoidiQ9VF30.
OMAiACANPQR.
OrthoDBiEOG7BW0HW.
PhylomeDBiQ9VF30.

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68689. CDC6 association with the ORC:origin complex.
R-DME-68827. CDT1 association with the CDC6:ORC:origin complex.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69300. Removal of licensing factors from origins.

Miscellaneous databases

GenomeRNAii49241.
PROiQ9VF30.

Gene expression databases

BgeeiQ9VF30.
GenevisibleiQ9VF30. DM.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR001208. MCM_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "REC, a new member of the MCM-related protein family, is required for meiotic recombination in Drosophila."
    Matsubayashi H., Yamamoto M.-T.
    Genes Genet. Syst. 78:363-371(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, VARIANTS HIS-118 AND ALA-422, MUTAGENESIS OF SER-455.
    Strain: Berkeley and Canton-S.
    Tissue: Ovary.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.

Entry informationi

Entry nameiMCMR_DROME
AccessioniPrimary (citable) accession number: Q9VF30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2002
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.