Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histone deacetylase complex subunit SAP18

Gene

Bin1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the tethering of the SIN3 complex to core histone proteins. Interacts with bicoid (bcd) to repress transcription of bicoid target genes in the anterior tip of the embryo; a process known as retraction. Interacts with Trl and binds to Polycomb response elements at the bithorax complex. May contribute to the regulation of other homeotic gene expressions.2 Publications

GO - Molecular functioni

  • transcription corepressor activity Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • chromatin silencing Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase complex subunit SAP18
Alternative name(s):
18 kDa Sin3-associated polypeptide
Bicoid-interacting protein 1
dSAP18
Gene namesi
Name:Bin1
Synonyms:SAP18
ORF Names:CG6046
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0024491. Bin1.

Subcellular locationi

  • Nucleus 2 Publications
  • Cytoplasm By similarity

  • Note: Shuttles between the nucleus and the cytoplasm.By similarity

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
  • Sin3 complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Histone deacetylase complex subunit SAP18PRO_0000220978Add
BLAST

Proteomic databases

PaxDbiQ9VEX9.
PRIDEiQ9VEX9.

Expressioni

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiQ9VEX9.
GenevisibleiQ9VEX9. DM.

Interactioni

Subunit structurei

Forms a complex with SIN3 and histone deacetylase (By similarity). Interacts with the N-terminal residues of TRL. Interacts with BCD; in vitro and yeast cells.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
bcdP090812EBI-129424,EBI-196628
CDC42P609532EBI-129424,EBI-81752From a different organism.
E(z)P421247EBI-129424,EBI-112315
PFY1P072743EBI-129424,EBI-13892From a different organism.
TrlQ08605-24EBI-129424,EBI-665803

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi67016. 25 interactions.
DIPiDIP-19160N.
IntActiQ9VEX9. 15 interactions.
MINTiMINT-806704.
STRINGi7227.FBpp0082731.

Structurei

3D structure databases

ProteinModelPortaliQ9VEX9.
SMRiQ9VEX9. Positions 16-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SAP18 family.Curated

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
GeneTreeiENSGT00390000003152.
InParanoidiQ9VEX9.
KOiK14324.
OMAiITPPNRM.
OrthoDBiEOG79SDZS.
PhylomeDBiQ9VEX9.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9VEX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVESMIVE EKTQVKQIDR EKTCPMLLRV FCSTGRHHSV SEYMFGNVPT
60 70 80 90 100
NELQIYTWQD ATLHELTSLV RDVNPDTRKK GTYFDFAVVY PNFRSNHFQM
110 120 130 140 150
REIGVTCTGQ KGIDDNKTLA QAKFSIGDFL DISITPPNRL PPTARRQRPY
Length:150
Mass (Da):17,288
Last modified:May 1, 2000 - v1
Checksum:i94947F206D8FA0AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571T → A in AAL48535 (PubMed:12537569).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297546 mRNA. Translation: AAG34996.1.
AE014297 Genomic DNA. Translation: AAF55284.1.
AY070913 mRNA. Translation: AAL48535.1.
BT025229 mRNA. Translation: ABF17920.1.
AJ278500 mRNA. Translation: CAB95728.1.
RefSeqiNP_001303440.1. NM_001316511.1.
NP_001303441.1. NM_001316512.1.
NP_524377.1. NM_079653.3.
UniGeneiDm.20197.

Genome annotation databases

EnsemblMetazoaiFBtr0083279; FBpp0082731; FBgn0024491.
FBtr0347200; FBpp0312505; FBgn0024491.
FBtr0347201; FBpp0312506; FBgn0024491.
GeneIDi41965.
KEGGidme:Dmel_CG6046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF297546 mRNA. Translation: AAG34996.1.
AE014297 Genomic DNA. Translation: AAF55284.1.
AY070913 mRNA. Translation: AAL48535.1.
BT025229 mRNA. Translation: ABF17920.1.
AJ278500 mRNA. Translation: CAB95728.1.
RefSeqiNP_001303440.1. NM_001316511.1.
NP_001303441.1. NM_001316512.1.
NP_524377.1. NM_079653.3.
UniGeneiDm.20197.

3D structure databases

ProteinModelPortaliQ9VEX9.
SMRiQ9VEX9. Positions 16-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67016. 25 interactions.
DIPiDIP-19160N.
IntActiQ9VEX9. 15 interactions.
MINTiMINT-806704.
STRINGi7227.FBpp0082731.

Proteomic databases

PaxDbiQ9VEX9.
PRIDEiQ9VEX9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083279; FBpp0082731; FBgn0024491.
FBtr0347200; FBpp0312505; FBgn0024491.
FBtr0347201; FBpp0312506; FBgn0024491.
GeneIDi41965.
KEGGidme:Dmel_CG6046.

Organism-specific databases

CTDi274.
FlyBaseiFBgn0024491. Bin1.

Phylogenomic databases

eggNOGiKOG3391. Eukaryota.
ENOG4111FBB. LUCA.
GeneTreeiENSGT00390000003152.
InParanoidiQ9VEX9.
KOiK14324.
OMAiITPPNRM.
OrthoDBiEOG79SDZS.
PhylomeDBiQ9VEX9.

Miscellaneous databases

GenomeRNAii41965.
NextBioi826507.
PROiQ9VEX9.

Gene expression databases

BgeeiQ9VEX9.
GenevisibleiQ9VEX9. DM.

Family and domain databases

InterProiIPR017250. Hist_deAcase_cplx_SAP18.
IPR010516. SAP18.
[Graphical view]
PANTHERiPTHR13082. PTHR13082. 1 hit.
PfamiPF06487. SAP18. 1 hit.
[Graphical view]
PIRSFiPIRSF037637. HDAC_SAP18. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila SAP18, a member of the Sin3/Rpd3 histone deacetylase complex, interacts with Bicoid and inhibits its activity."
    Zhu W., Foehr M., Jaynes J.B., Hanes S.D.
    Dev. Genes Evol. 211:109-117(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH BCD, DEVELOPMENTAL STAGE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
  6. "The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide."
    Espinas M.L., Canudas S., Fanti L., Pimpinelli S., Casanova J., Azorin F.
    EMBO Rep. 1:253-259(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-150, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TRL.

Entry informationi

Entry nameiSAP18_DROME
AccessioniPrimary (citable) accession number: Q9VEX9
Secondary accession number(s): Q1LYZ7, Q8SZF6, Q9N9Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.