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Q9VET0

- NPF_DROME

UniProt

Q9VET0 - NPF_DROME

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Protein

Neuropeptide F

Gene

NPF

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference.7 Publications

GO - Molecular functioni

  1. neuropeptide F receptor binding Source: FlyBase
  2. neuropeptide hormone activity Source: UniProtKB

GO - Biological processi

  1. circadian behavior Source: FlyBase
  2. circadian rhythm Source: FlyBase
  3. digestion Source: UniProtKB-KW
  4. G-protein coupled receptor signaling pathway Source: FlyBase
  5. larval feeding behavior Source: FlyBase
  6. larval foraging behavior Source: UniProtKB
  7. larval locomotory behavior Source: FlyBase
  8. locomotor rhythm Source: FlyBase
  9. male courtship behavior Source: FlyBase
  10. neuropeptide signaling pathway Source: FlyBase
  11. regulation of response to food Source: UniProtKB
  12. response to stress Source: UniProtKB-KW
  13. social behavior Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Neuropeptide

Keywords - Biological processi

Behavior, Digestion, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropeptide F
Short name:
NPF
Alternative name(s):
dNPF
Gene namesi
Name:NPF
ORF Names:CG10342
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0027109. NPF.

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Increased NPF or NPFR activity dominantly suppresses PAIN-mediated food aversion in postfeeding larvae. Deficiency in NPF/NPFR signaling causes decreased alcohol sensitivity and overexpression causes a hypersensitive response to alcohol sedation. Controlled functional disruption of NPF or NPFR neurons rapidly triggers acute resistance to ethanol sedation.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Propeptidei27 – 3261 PublicationPRO_0000283079
Peptidei35 – 6228Neuropeptide F1 PublicationPRO_0000283080Add
BLAST
Propeptidei66 – 102371 PublicationPRO_0000283081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621Phenylalanine amide1 Publication

Keywords - PTMi

Amidation, Cleavage on pair of basic residues

Proteomic databases

PRIDEiQ9VET0.

Expressioni

Tissue specificityi

Expressed in midgut, brain lobes and ventral nerve cord of larvae. Predominantly expressed in two pairs of protocerebral neurons in the larval CNS (at protein level). Intense expression is also seen in the fan-shaped body of the central complex and two lateral areas of the lower part of the central brain that appear to harbor the giant commissural interneurons of the giant fiber pathway (at protein level). Upon glucose feeding, two additional dNPFergic neurons are consistently detected on the ventromedial surface of the subesophageal ganglion (SEG) of third instars larvae. Expressed in a subset of sugar-responsive PAIN neurons in the thoracic body but is absent from other peripheral PAIN neurons.6 Publications

Developmental stagei

Expression is high in larvae seeking food and is down-regulated in late embryos coinciding with the onset of the behavior of older larvae, including food aversion, hypermobility, and cooperative burrowing. In males, expression is increased by mating and reduced by sexual deprivation.1 Publication

Gene expression databases

BgeeiQ9VET0.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0082778.

Structurei

3D structure databases

ProteinModelPortaliQ9VET0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NPY family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG266214.
InParanoidiQ9VET0.
OMAiPPRNNEI.
OrthoDBiEOG7W41F1.
PhylomeDBiQ9VET0.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VET0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCQTMRCILV ACVALALLAA GCRVEASNSR PPRKNDVNTM ADAYKFLQDL
60 70 80 90 100
DTYYGDRARV RFGKRGSLMD ILRNHEMDNI NLGKNANNGG EFARGFNEEE

IF
Length:102
Mass (Da):11,465
Last modified:May 1, 2000 - v1
Checksum:iD6403F3C5DA7D79F
GO

Sequence cautioni

The sequence AAD42053.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591R → A AA sequence (PubMed:10499420)Curated
Sequence conflicti61 – 611R → V AA sequence (PubMed:10499420)Curated
Sequence conflicti70 – 701D → E in AAD42053. (PubMed:10499420)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117896 Genomic DNA. Translation: AAD42053.1. Sequence problems.
AE014297 Genomic DNA. Translation: AAF55339.1.
BT023797 mRNA. Translation: AAZ41806.1.
RefSeqiNP_001262642.1. NM_001275713.1.
NP_001262643.1. NM_001275714.1.
NP_536741.1. NM_080493.3.
UniGeneiDm.5729.

Genome annotation databases

EnsemblMetazoaiFBtr0083328; FBpp0082778; FBgn0027109.
FBtr0331685; FBpp0304074; FBgn0027109.
FBtr0331686; FBpp0304075; FBgn0027109.
GeneIDi42018.
KEGGidme:Dmel_CG10342.

Cross-referencesi

Web resourcesi

Protein Spotlight

On sex, drugs and satisfaction - Issue 138 of May 2012

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117896 Genomic DNA. Translation: AAD42053.1 . Sequence problems.
AE014297 Genomic DNA. Translation: AAF55339.1 .
BT023797 mRNA. Translation: AAZ41806.1 .
RefSeqi NP_001262642.1. NM_001275713.1.
NP_001262643.1. NM_001275714.1.
NP_536741.1. NM_080493.3.
UniGenei Dm.5729.

3D structure databases

ProteinModelPortali Q9VET0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7227.FBpp0082778.

Proteomic databases

PRIDEi Q9VET0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0083328 ; FBpp0082778 ; FBgn0027109 .
FBtr0331685 ; FBpp0304074 ; FBgn0027109 .
FBtr0331686 ; FBpp0304075 ; FBgn0027109 .
GeneIDi 42018.
KEGGi dme:Dmel_CG10342.

Organism-specific databases

CTDi 42018.
FlyBasei FBgn0027109. NPF.

Phylogenomic databases

eggNOGi NOG266214.
InParanoidi Q9VET0.
OMAi PPRNNEI.
OrthoDBi EOG7W41F1.
PhylomeDBi Q9VET0.

Miscellaneous databases

GenomeRNAii 42018.
NextBioi 826764.

Gene expression databases

Bgeei Q9VET0.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family."
    Brown M.R., Crim J.W., Arata R.C., Cai H.N., Chun C., Shen P.
    Peptides 20:1035-1042(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-62, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AMIDATION AT PHE-62.
    Strain: Oregon-R1 Publication.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley1 Publication.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BerkeleyImported.
    Tissue: Head.
  5. "Drosophila neuropeptide F mediates integration of chemosensory stimulation and conditioning of the nervous system by food."
    Shen P., Cai H.N.
    J. Neurobiol. 47:16-25(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. "Developmental control of foraging and social behavior by the Drosophila neuropeptide Y-like system."
    Wu Q., Wen T., Lee G., Park J.H., Cai H.N., Shen P.
    Neuron 39:147-161(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  7. "Drosophila neuropeptide F and its receptor, NPFR1, define a signaling pathway that acutely modulates alcohol sensitivity."
    Wen T., Parrish C.A., Xu D., Wu Q., Shen P.
    Proc. Natl. Acad. Sci. U.S.A. 102:2141-2146(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  8. "A neural circuit mechanism integrating motivational state with memory expression in Drosophila."
    Krashes M.J., DasGupta S., Vreede A., White B., Armstrong J.D., Waddell S.
    Cell 139:416-427(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  9. "A G-protein-coupled neuropeptide Y-like receptor suppresses behavioral and sensory response to multiple stressful stimuli in Drosophila."
    Xu J., Li M., Shen P.
    J. Neurosci. 30:2504-2512(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  10. "Sexual deprivation increases ethanol intake in Drosophila."
    Shohat-Ophir G., Kaun K.R., Azanchi R., Heberlein U.
    Science 335:1351-1355(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiNPF_DROME
AccessioniPrimary (citable) accession number: Q9VET0
Secondary accession number(s): Q9Y1K3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 1, 2000
Last modified: October 1, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3