Reviewed, UniProtKB/Swiss-Prot Q9VE01 (C12A5_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 12a5, mitochondrial
    EC=1.14.-.-
Alternative name(s):
    CYPXIIA5

Gene names

Name:	Cyp12a5
ORF Names:	CG11821

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 536		Probable cytochrome P450 12a5, mitochondrial		PRO_0000003609
Sites
	Metal binding	482	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	66	1	G → S in AAM76202. Ref.4
	Sequence conflict	72	1	L → M in AAR88135. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9VE01-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 231BFD7E6284A1F6	FASTA	536	61,355
	10 20 30 40 50 60 MLKGRIALNI LQSQKPIVFS ASQQRWQTNV PTAEIRNDPE WLQAKPFEEI PKANILSLFA 70 80 90 100 110 120 KSALPGGKYK NLEMMEMIDA LRQDYGNIIF LPGMMGRDGL VMTHNPKDFE VVFRNEGVWP 130 140 150 160 170 180 FRPGSDILRY HRTVYRKDFF DGVQGIIPSQ GKSWGDFRSI VNPVLMQPKN VRLYFKKMSQ 190 200 210 220 230 240 VNQEFVELIK EIRDASTQEV PGNFLETINR WTLESVSVVA LDKQLGLLRE SGKNSEATKL 250 260 270 280 290 300 FKYLDEFFLH SADLEMKPSL WRYFKTPLLK KMLRTMDSVQ EVTLKYVDEA IERLEKEAKE 310 320 330 340 350 360 GVVRPEHEQS VLEKLLKVDK KVATVMAMDM LMAGVDTTSS TFTALLLCLA KNPEKQARLR 370 380 390 400 410 420 EEVMKVLPNK DSEFTEASMK NVPYLRACIK ESQRVYPLVI GNARGLTRDS VISGYRVPAG 430 440 450 460 470 480 TIVSMIPINS LYSEEYFPKP TEFLPERWLR NASDSAGKCP ANDLKTKNPF VFLPFGFGPR 490 500 510 520 530 MCVGKRIVEM ELELGTARLI RNFNVEFNHS TKNAFRSALI NLPNIPLKFK FTDVPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Cyp6g1 and Cyp12d1 in DDT resistant and susceptible strains of Drosophila melanogaster." Festucci-Buselli R.A., Carvalho-Dias A.S., de Oliveira-Andrade M., Caixeta-Nunes C., Li H.-M., Stuart J.J., Muir W., Scharf M.E., Pittendrigh B.R. Insect Mol. Biol. 14:69-77(2005) [PubMed: 15663776] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY459354 mRNA. Translation: AAR88135.1. AE014297 Genomic DNA. Translation: AAF55635.1. AY129460 mRNA. Translation: AAM76202.1.
RefSeq	NP_650782.1.
UniGene	Dm.13716
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:20196N.
IntAct	Q9VE01. 1 interaction.
Proteomic databases
PRIDE	Q9VE01.
Genome annotation databases
Ensembl	FBgn0038680. Drosophila melanogaster. [Contig view]
GeneID	42293.
KEGG	dme:Dmel_CG11821.
NMPDR	fig|7227.3.peg.13557.
Organism-specific databases
FlyBase	FBgn0038680. Cyp12a5.
Phylogenomic databases
HOGENOM	Q9VE01.
OMA	Q9VE01. KDSEFTE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-012323-MON.
Gene expression databases
ArrayExpress	Q9VE01.
GermOnline	CG11821. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	828077.

Entry information

Entry name

C12A5_DROME

Accession

Primary (citable) accession number: Q9VE01
Secondary accession number(s): Q6SEI2, Q8MQI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project