Reviewed, UniProtKB/Swiss-Prot Q9VE00 (C12A4_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 12a4, mitochondrial
    EC=1.14.-.-
Alternative name(s):
    CYPXIIA4

Gene names

Name:	Cyp12a4
ORF Names:	CG6042

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in resistance to insecticide lufenuron, but no other insecticides. Ref.2
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane Potential.
Tissue specificity	Expression in third-instar larvae is detected in the midgut and Malpighian tubules of both lufenuron-resistant and wild-type strains, higher expression level is seen in lufenuron-resistant strains. Ref.2
Miscellaneous	Bari1 transposable element insertion in the 3' end of the gene leads to ten-fold more abundant expression than in flies lacking the insert.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to insecticide Ref.2 Inferred from mutant phenotype. Source: FlyBase
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RpL37A	Q9VMU4	1	EBI-152246,EBI-144060

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 536		Probable cytochrome P450 12a4, mitochondrial		PRO_0000003608
Sites
	Metal binding	482	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	208	1	I → V in strain: Subline 5. Ref.1
	Natural variant	252	1	I → T in strain: Subline 5 and Subline 7. Ref.1
	Natural variant	289	1	E → G in strain: Subline 5. Ref.1
	Natural variant	295	1	D → G in strain: Subline 8. Ref.1
	Natural variant	417	1	V → A in strain: Subline 8. Ref.1
	Natural variant	499	1	L → P in strain: Subline 7. Ref.1
Experimental info
	Sequence conflict	203	1	D → H in AAL48134. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9VE00-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: DDE51C7726A8805E	FASTA	536	61,777
	10 20 30 40 50 60 MLKVRSALSL IQSQKATLSL ATQKRWQTNV ATAEAREDSE WLQAKPFEQI PRLNMWALSM 70 80 90 100 110 120 KMSMPGGKYK NMELMEMFEA MRQDYGDIFF MPGIMGNPPF LSTHNPQDFE VVFRNEGVWP 130 140 150 160 170 180 NRPGNYTLLY HREEYRKDFY QGVMGVIPTQ GKPWGDFRTV VNPVLMQPKN VRLYYKKMSQ 190 200 210 220 230 240 VNQEFVQRIL ELRDPDTLEA PDDFIDTINR WTLESVSVVA LDKQLGLLKN SNKESEALKL 250 260 270 280 290 300 FHYLDEFFIV SIDLEMKPSP WRYIKTPKLK RLMRALDGIQ EVTLAYVDEA IERLDKEAKE 310 320 330 340 350 360 GVVRPENEQS VLEKLLKVDR KVATVMAMDM LMAGVDTTSS TFTALLLCLA KNPEKQARLR 370 380 390 400 410 420 EEVMKVLPNK NSEFTEASMK NVPYLRACIK ESQRLHPLIV GNARVLARDA VLSGYRVPAG 430 440 450 460 470 480 TYVNIVPLNA LTRDEYFPQA SEFLPERWLR SPKDSESKCP ANELKSTNPF VFLPFGFGPR 490 500 510 520 530 MCVGKRIVEM ELELGTARLI RNFNVEFNYP TENAFRSALI NLPNIPLKFK FIDLPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for a functional interaction between the Bari1 transposable element and the cytochrome P450 cyp12a4 gene in Drosophila melanogaster." Marsano R.M., Caizzi R., Moschetti R., Junakovic N. Gene 357:122-128(2005) [PubMed: 16076534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS VAL-208; THR-252; GLY-289; GLY-295; ALA-417 AND PRO-499. Strain: Subline 5, Subline 7 and Subline 8.
[2]	"Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster." Bogwitz M.R., Chung H., Magoc L., Rigby S., Wong W., O'Keefe M., McKenzie J.A., Batterham P., Daborn P.J. Proc. Natl. Acad. Sci. U.S.A. 102:12807-12812(2005) [PubMed: 16120680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY. Strain: NB16.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[6]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ748833 Genomic DNA. Translation: CAG38967.1. AJ748834 Genomic DNA. Translation: CAG38968.1. AJ890254 mRNA. Translation: CAI64834.1. DQ026292 Genomic DNA. Translation: AAY89653.1. AE014297 Genomic DNA. Translation: AAF55636.2. AY070663 mRNA. Translation: AAL48134.1. BT056257 mRNA. Translation: ACL68704.1.
RefSeq	NP_650783.2.
UniGene	Dm.31262
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:23927N.
IntAct	Q9VE00. 1 interaction.
Proteomic databases
PRIDE	Q9VE00.
Genome annotation databases
Ensembl	FBgn0038681. Drosophila melanogaster. [Contig view]
GeneID	42294.
KEGG	dme:Dmel_CG6042.
NMPDR	fig|7227.3.peg.13558.
Organism-specific databases
FlyBase	FBgn0038681. Cyp12a4.
Phylogenomic databases
HOGENOM	Q9VE00.
OMA	Q9VE00. NIPANDL.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-012324-MON.
Gene expression databases
ArrayExpress	Q9VE00.
GermOnline	CG6042. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	828082.

Entry information

Entry name

C12A4_DROME

Accession

Primary (citable) accession number: Q9VE00
Secondary accession number(s): B8A3W5 , Q461P8, Q5CAL0, Q6A1J1, Q6A1J2, Q8SZL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	July 7, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project