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Protein

Probable ATP-dependent RNA helicase pitchoune

Gene

pit

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probable RNA-dependent helicase. Functions in cell growth and proliferation. May have a role in ribosome biogenesis and, consequently, in protein biosynthesis.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi231 – 2388ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA binding Source: UniProtKB-KW
  • RNA helicase activity Source: FlyBase

GO - Biological processi

  • cell growth Source: FlyBase
  • cell proliferation Source: FlyBase
  • RNA secondary structure unwinding Source: GO_Central
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase pitchoune (EC:3.6.4.13)
Gene namesi
Name:pit
ORF Names:CG6375
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0266581. pit.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 680680Probable ATP-dependent RNA helicase pitchounePRO_0000055003Add
BLAST

Proteomic databases

PaxDbiQ9VD51.

Expressioni

Gene expression databases

BgeeiFBgn0025140.
GenevisibleiQ9VD51. DM.

Interactioni

Protein-protein interaction databases

BioGridi67556. 24 interactions.
DIPiDIP-19700N.
IntActiQ9VD51. 6 interactions.
MINTiMINT-1004790.
STRINGi7227.FBpp0083572.

Structurei

3D structure databases

ProteinModelPortaliQ9VD51.
SMRiQ9VD51. Positions 186-553.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini218 – 393176Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini407 – 577171Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 21529Q motifAdd
BLAST
Motifi341 – 3444DEVD box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00680000100037.
InParanoidiQ9VD51.
KOiK13179.
OMAiIQHKTIR.
OrthoDBiEOG091G06N7.
PhylomeDBiQ9VD51.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VD51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIREKLLMK KIVKREKMKK ELSQKKGNKN AQKQEPPKQN GNKPSKKPEK
60 70 80 90 100
LSKKHVAKDE DDDLEEDFQE APLPKKKQQK QPPKKQQIQV ANSDSESDDD
110 120 130 140 150
EQEDEADEDS DLDEVAEVDE EDVDSGSEDD DQQEDEDEEE PVPAKKTKLL
160 170 180 190 200
PNKSKAQNGK PAKDDEPFTV ESSLAALDYR DSDDRSFASL KGAVSEATLR
210 220 230 240 250
AIKEMGFTEM TEIQSKSLTP LLKGRDLVGA AQTGSGKTLA FLIPAVELIN
260 270 280 290 300
KLRFMPRNGT GVIIISPTRE LSMQTFGVLK ELMAHHHHTY GLVMGGSNRQ
310 320 330 340 350
VESEKLGKGI NILVATPGRL LDHLQNSPDF LYKNLQCLII DEVDRILEIG
360 370 380 390 400
FEEELKQIIN LLPKRRQTML FSATQTARIE ALSKLALKSE PIYVGVHDNQ
410 420 430 440 450
DTATVDGLEQ GYIVCPSEKR LLVLFTFLKK NRKKKVMVFF SSCMSVKYHH
460 470 480 490 500
ELFNYIDLPV TSIHGKQKQT KRTTTFFQFC NAESGILLCT DVAARGLDIP
510 520 530 540 550
QVDWIVQYDP PDDPREYIHR VGRTARGSGT SGHALLLMRP EELGFLRYLK
560 570 580 590 600
AAKVPLNEFE FSWQKIADIQ LQLEKLIAKN YFLNQSAKEA FKSYVRAYDS
610 620 630 640 650
HQLKQIFNVN TLDLQAVAKS FGFLVPPVVD LKVGAAKRER PEKRVGGGGF
660 670 680
GFYKKMNEGS ASKQRHFKQV NRDQAKKFMR
Length:680
Mass (Da):76,928
Last modified:August 15, 2003 - v2
Checksum:iE71CD18B8A135FF1
GO

Sequence cautioni

The sequence AAC27683 differs from that shown. Reason: Frameshift at position 3. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 301N → K in AAC27683 (PubMed:9716523).Curated
Sequence conflicti115 – 1151V → A in AAC27683 (PubMed:9716523).Curated
Sequence conflicti512 – 5176DDPREY → GDQASI in AAC27683 (PubMed:9716523).Curated
Sequence conflicti634 – 64411GAAKRERPEKR → ARPSGSDRKSD in AAC27683 (PubMed:9716523).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84552 mRNA. Translation: AAC27683.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF55951.2.
AE014297 Genomic DNA. Translation: AAN13900.2.
AY071402 mRNA. Translation: AAL49024.1.
AY119620 mRNA. Translation: AAM50274.1.
RefSeqiNP_001287459.1. NM_001300530.1.
NP_524446.3. NM_079722.4.
NP_732694.2. NM_169991.2.
UniGeneiDm.33428.

Genome annotation databases

EnsemblMetazoaiFBtr0084174; FBpp0083572; FBgn0266581.
FBtr0084175; FBpp0083573; FBgn0266581.
FBtr0346345; FBpp0312062; FBgn0266581.
GeneIDi42595.
KEGGidme:Dmel_CG6375.
UCSCiCG6375-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84552 mRNA. Translation: AAC27683.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF55951.2.
AE014297 Genomic DNA. Translation: AAN13900.2.
AY071402 mRNA. Translation: AAL49024.1.
AY119620 mRNA. Translation: AAM50274.1.
RefSeqiNP_001287459.1. NM_001300530.1.
NP_524446.3. NM_079722.4.
NP_732694.2. NM_169991.2.
UniGeneiDm.33428.

3D structure databases

ProteinModelPortaliQ9VD51.
SMRiQ9VD51. Positions 186-553.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67556. 24 interactions.
DIPiDIP-19700N.
IntActiQ9VD51. 6 interactions.
MINTiMINT-1004790.
STRINGi7227.FBpp0083572.

Proteomic databases

PaxDbiQ9VD51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084174; FBpp0083572; FBgn0266581.
FBtr0084175; FBpp0083573; FBgn0266581.
FBtr0346345; FBpp0312062; FBgn0266581.
GeneIDi42595.
KEGGidme:Dmel_CG6375.
UCSCiCG6375-RA. d. melanogaster.

Organism-specific databases

CTDi42595.
FlyBaseiFBgn0266581. pit.

Phylogenomic databases

eggNOGiKOG0342. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00680000100037.
InParanoidiQ9VD51.
KOiK13179.
OMAiIQHKTIR.
OrthoDBiEOG091G06N7.
PhylomeDBiQ9VD51.

Miscellaneous databases

ChiTaRSipit. fly.
GenomeRNAii42595.
PROiQ9VD51.

Gene expression databases

BgeeiFBgn0025140.
GenevisibleiQ9VD51. DM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR025313. DUF4217.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF13959. DUF4217. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM01178. DUF4217. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX18_DROME
AccessioniPrimary (citable) accession number: Q9VD51
Secondary accession number(s): A4V384, O77001, Q8SYP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 15, 2003
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Mutation in the pit gene produce larvae that cannot grow beyond the first instar larval stage although they can live as long as 7-10 days. All the tissues are equally affected and the perfectly shaped larvae are indistinguishable from first instar wild-type animals.
'Pitchoune' means 'small' in Provence (Southern part of France).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.