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Reviewed, UniProtKB/Swiss-Prot Q9VD51 (DDX18_DROME)

Last modified June 16, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable ATP-dependent RNA helicase pitchoune
    EC=3.6.1.-
Gene names
Name: pit
ORF Names: CG6375
OrganismDrosophila melanogaster (Fruit fly) [Complete proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

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Protein attributes

Sequence length680 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Probable RNA-dependent helicase. Functions in cell growth and proliferation. May have a role in ribosome biogenesis and, consequently, in protein biosynthesis. Ref.1

Subcellular location

Nucleus › nucleolus. Ref.1

Miscellaneous

Mutation in the pit gene produce larvae that cannot grow beyond the first instar larval stage although they can live as long as 7-10 days. All the tissues are equally affected and the perfectly shaped larvae are indistinguishable from first instar wild-type animals.

'Pitchoune' means 'small' in Provence (Southern part of France).

Sequence similarities

Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence AAC27683.1 differs from that shown. Reason: Frameshift at position 3.

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Ontologies

Keywords
   Cellular componentNucleus
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentnucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent helicase activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 680680Probable ATP-dependent RNA helicase pitchoune
PRO_0000055003

Regions

Domain218 – 393176Helicase ATP-binding
Domain407 – 577171Helicase C-terminal
Nucleotide binding231 – 2388ATP By similarity
Motif187 – 21529Q motif
Motif341 – 3444DEVD box

Experimental info

Sequence conflict301N → K in AAC27683. Ref.1
Sequence conflict1151V → A in AAC27683. Ref.1
Sequence conflict512 – 5176DDPREY → GDQASI in AAC27683. Ref.1
Sequence conflict634 – 64411GAAKRERPEKR → ARPSGSDRKSD in AAC27683. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q9VD51-1 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: E71CD18B8A135FF1

FASTA68076,928
        10         20         30         40         50         60 
MSIREKLLMK KIVKREKMKK ELSQKKGNKN AQKQEPPKQN GNKPSKKPEK LSKKHVAKDE 

        70         80         90        100        110        120 
DDDLEEDFQE APLPKKKQQK QPPKKQQIQV ANSDSESDDD EQEDEADEDS DLDEVAEVDE 

       130        140        150        160        170        180 
EDVDSGSEDD DQQEDEDEEE PVPAKKTKLL PNKSKAQNGK PAKDDEPFTV ESSLAALDYR 

       190        200        210        220        230        240 
DSDDRSFASL KGAVSEATLR AIKEMGFTEM TEIQSKSLTP LLKGRDLVGA AQTGSGKTLA 

       250        260        270        280        290        300 
FLIPAVELIN KLRFMPRNGT GVIIISPTRE LSMQTFGVLK ELMAHHHHTY GLVMGGSNRQ 

       310        320        330        340        350        360 
VESEKLGKGI NILVATPGRL LDHLQNSPDF LYKNLQCLII DEVDRILEIG FEEELKQIIN 

       370        380        390        400        410        420 
LLPKRRQTML FSATQTARIE ALSKLALKSE PIYVGVHDNQ DTATVDGLEQ GYIVCPSEKR 

       430        440        450        460        470        480 
LLVLFTFLKK NRKKKVMVFF SSCMSVKYHH ELFNYIDLPV TSIHGKQKQT KRTTTFFQFC 

       490        500        510        520        530        540 
NAESGILLCT DVAARGLDIP QVDWIVQYDP PDDPREYIHR VGRTARGSGT SGHALLLMRP 

       550        560        570        580        590        600 
EELGFLRYLK AAKVPLNEFE FSWQKIADIQ LQLEKLIAKN YFLNQSAKEA FKSYVRAYDS 

       610        620        630        640        650        660 
HQLKQIFNVN TLDLQAVAKS FGFLVPPVVD LKVGAAKRER PEKRVGGGGF GFYKKMNEGS 

       670        680 
ASKQRHFKQV NRDQAKKFMR

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References

« Hide 'large scale' references
[1]"A Drosophila RNA helicase gene, pitchoune, is required for cell growth and proliferation and is a potential target of d-Myc."
Zaffran S., Chartier A., Gallant P., Astier M., Arquier N., Doherty D., Gratecos D., Semeriva M.
Development 125:3571-3584(1998) [PubMed: 9716523] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: Oregon-R.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract]
Cited for: GENOME REANNOTATION.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.

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Cross-references

Sequence databases

U84552 mRNA. Translation: AAC27683.1. Frameshift.
AE014297 Genomic DNA. Translation: AAF55951.2.
AE014297 Genomic DNA. Translation: AAN13900.2.
AY071402 mRNA. Translation: AAL49024.1.
AY119620 mRNA. Translation: AAM50274.1.
RefSeqNP_524446.3.
NP_732694.2.
UniGeneDm.33428

3D structure databases

HSSPHSSP built from PDB template 1HV8 based on UniProtKB Q58083.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:19700N.
IntActQ9VD51. 1 interaction.

Genome annotation databases

EnsemblFBgn0025140. Drosophila melanogaster. [Contig view]
GeneID42595.
KEGGdme:Dmel_CG6375.

Organism-specific databases

FlyBaseFBgn0025140. pit.

Phylogenomic databases

HOGENOMQ9VD51.
OMAQ9VD51. GFEDEMR.

Enzyme and pathway databases

BioCycDMEL-XXX-02:DMEL-XXX-02-012758-MON.
DMEL-XXX-02:DMEL-XXX-02-012759-MON.

Gene expression databases

ArrayExpressQ9VD51.
GermOnlineCG6375. Drosophila melanogaster.

Family and domain databases

InterProIPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014021. Helicase_SF1/SF2_ATP-bd.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00039. DEAD_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio829597.

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Entry information

Entry nameDDX18_DROME
AccessionPrimary (citable) accession number: Q9VD51
Secondary accession number(s): A4V384, O77001, Q8SYP5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 15, 2003
Last modified: June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectDrosophila annotation project

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Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents