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Q9VD44

- GLD2A_DROME

UniProt

Q9VD44 - GLD2A_DROME

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Protein

Poly(A) RNA polymerase gld-2 homolog A

Gene

Gld2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi993 – 9931Magnesium or manganese; catalyticBy similarity
Metal bindingi995 – 9951Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. long-term memory Source: FlyBase
  2. mRNA processing Source: UniProtKB-KW
  3. RNA polyadenylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-2 homolog A (EC:2.7.7.19)
Short name:
DmGLD2
Gene namesi
Name:Gld2
ORF Names:CG5732
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038934. Gld2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Found in neuronal cytoplasm, present in discrete neuronal particles associated with mRNA control, and in nuclear puncta.

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. neuron projection Source: FlyBase
  3. nucleoplasm Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi995 – 9951D → A: Destroys active site. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13641364Poly(A) RNA polymerase gld-2 homolog APRO_0000341557Add
BLAST

Proteomic databases

PaxDbiQ9VD44.

Expressioni

Tissue specificityi

Expressed in the brain.1 Publication

Developmental stagei

Expressed in larvae, pupae and adults.1 Publication

Gene expression databases

BgeeiQ9VD44.

Interactioni

Subunit structurei

Interacts with Fmr1 and eIF-4E.1 Publication

Protein-protein interaction databases

BioGridi67563. 2 interactions.
IntActiQ9VD44. 2 interactions.
MINTiMINT-1691551.

Structurei

3D structure databases

ProteinModelPortaliQ9VD44.
SMRiQ9VD44. Positions 941-1251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1163 – 122462PAP-associatedAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 118Poly-Ala
Compositional biasi15 – 206Poly-Ala
Compositional biasi465 – 49127Ser-richAdd
BLAST
Compositional biasi599 – 64547Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
InParanoidiQ9VD44.
KOiK14079.
OMAiNRYWREF.
OrthoDBiEOG7DRJ2S.
PhylomeDBiQ9VD44.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q9VD44-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAMAMAAA ASSSAAAATA TTTAISNSTN TTASPATTNT INTTTKTITT
60 70 80 90 100
SEVPLEPASN MAPKALEIED SGRNEPEDLE HGAAKPLEQR KTTVVHTCPR
110 120 130 140 150
PPGGYKYSME FLYGIGSGMA GIPLNIPTPS SITPRTVRST APLLTTHMPL
160 170 180 190 200
LTNMGVAPSR PSGIRYPGSA GGSNGTTVAA STTPSTTVTT SSGSPGSGAE
210 220 230 240 250
AITSSVSSSG LPAAGLQAAQ FICQGYMPTG PQRRLWHAEN AVWQFDRNYP
260 270 280 290 300
YNQAYSPPYG IPMMPVGFEH PYGQRIIYPG YYNQTPPGIN PAAVAGLTRT
310 320 330 340 350
NRPVTQQPHI LTQPAVGTTE SSEEAPATLG NAPQVSSTWR YGRPGTHRGR
360 370 380 390 400
HAVPTAAAPA LLHRDSKSFY NSIGSGMANG PRFKAPFVAN VRNFQAGAVA
410 420 430 440 450
TVAAGGAATT AVVGTSAPAT GAASSSDQNV ATKRNHQGAA TQNNHRNRHN
460 470 480 490 500
AKKGGKNSVG KELTSNSSES LSNSSSKSQL NKRPSSSSSI SPIKHPHRNY
510 520 530 540 550
RNRMRYTATE PTEQKAATTT VPISNYQPPQ QSTSTVRRTS KFQGSNAYQA
560 570 580 590 600
HTAAGRQQSR FYQSRHMDGY VFQSGHYMVY AAGAPPVGLR PGKSPVSEAT
610 620 630 640 650
GAPPGAAAAT AAAATAVAAT AAAAEGGGAA EGGGAAAAPV TASSATLEGE
660 670 680 690 700
QPLDSDFDQR QEFADLGLDP ANGGFSSDLE PNGIKQDTSE LDTHSCLISH
710 720 730 740 750
PNSEVDGDDN QSLASFAPSV ESDDSDSELS DASVESVARD ILVSCLAVAT
760 770 780 790 800
GAEEPDFSGP NLVPYGDMHY LKELDKKNPP TNGYRSHRPY HQSHYAYHSQ
810 820 830 840 850
MSPRGLSCCG DMLNQHSEDL VFKLDQNQPD GIESGKNIFL REITEQPDNI
860 870 880 890 900
SVASNLSCSP SASSSKSVLA PMASKSNITM PEENNDDDEL PLVVHNRYWR
910 920 930 940 950
EFFGYTPADR FLLRAKFVEM RRPPKVMGCK NKWDPLSLSV WKKFLESQQT
960 970 980 990 1000
RHVYKIKMRL WRAIYTVAMK NYPRYGLYLV GSSISYFGSK CSDMDICMLA
1010 1020 1030 1040 1050
CTNPNIDSRM EAVYHLHVMK ELLGRTNMFQ DFNLIEARVP ILRFTDRCHK
1060 1070 1080 1090 1100
VEVDINFNNS VGIRNTHLLY CYSQLDWRVR PMALTVKQWA QYHNINNAKN
1110 1120 1130 1140 1150
MTISSYSLML MVIHFLQVGA SPPVLPCLHN LYPEKFGLLQ PNDFGYVDMN
1160 1170 1180 1190 1200
EVMAPYQSDN SQTLGDLLLS FLHYYSVFDY GKYAISIRVG GVLPIEVCRA
1210 1220 1230 1240 1250
ATAPKNDIHQ WNELCIEEPF DQTNTARSVY DTDTFERIKT IFVASYRRLD
1260 1270 1280 1290 1300
STRNLSAIFE DYDGPTILMQ QPSVDSEIEL YEGQHHRLLP NRGSSRSNSA
1310 1320 1330 1340 1350
IPSPRPSILM VDKATTAIWD DINNKPDHPV LSHSNNYDAT NECTGNGSLM
1360
GLKDNSVADK PPIA

Note: No experimental confirmation available.

Length:1,364
Mass (Da):147,592
Last modified:July 5, 2004 - v3
Checksum:i9BAFCAE8D75083CA
GO
Isoform B (identifier: Q9VD44-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     881-1364: PEENNDDDEL...NSVADKPPIA → VGELKNVLLN...LIVHFIRFTK

Note: No experimental confirmation available.

Show »
Length:919
Mass (Da):96,248
Checksum:i35B7D4D987E439A2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti523 – 5231I → V in AAM52588. (PubMed:12537569)Curated
Sequence conflicti523 – 5231I → V in ACH95257. 1 PublicationCurated
Sequence conflicti591 – 5911P → A in AAM52588. (PubMed:12537569)Curated
Sequence conflicti591 – 5911P → A in ACH95257. 1 PublicationCurated
Sequence conflicti610 – 6101T → TAA in AAM52588. (PubMed:12537569)Curated
Sequence conflicti610 – 6101T → TAA in ACH95257. 1 PublicationCurated
Sequence conflicti636 – 6361A → T in AAM52588. (PubMed:12537569)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei881 – 1364484PEENN…KPPIA → VGELKNVLLNGQITKFISSK SYFFLSSVCLIVHFIRFTK in isoform B. 1 PublicationVSP_034331Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55959.3.
AY122076 mRNA. Translation: AAM52588.1.
BT044483 mRNA. Translation: ACH95257.1.
RefSeqiNP_001262829.1. NM_001275900.1. [Q9VD44-1]
NP_651012.2. NM_142755.4. [Q9VD44-1]
UniGeneiDm.33737.

Genome annotation databases

EnsemblMetazoaiFBtr0305038; FBpp0293575; FBgn0038934. [Q9VD44-1]
FBtr0310204; FBpp0301888; FBgn0038934. [Q9VD44-1]
GeneIDi42602.
KEGGidme:Dmel_CG5732.
UCSCiCG5732-RA. d. melanogaster. [Q9VD44-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55959.3 .
AY122076 mRNA. Translation: AAM52588.1 .
BT044483 mRNA. Translation: ACH95257.1 .
RefSeqi NP_001262829.1. NM_001275900.1. [Q9VD44-1 ]
NP_651012.2. NM_142755.4. [Q9VD44-1 ]
UniGenei Dm.33737.

3D structure databases

ProteinModelPortali Q9VD44.
SMRi Q9VD44. Positions 941-1251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 67563. 2 interactions.
IntActi Q9VD44. 2 interactions.
MINTi MINT-1691551.

Proteomic databases

PaxDbi Q9VD44.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0305038 ; FBpp0293575 ; FBgn0038934 . [Q9VD44-1 ]
FBtr0310204 ; FBpp0301888 ; FBgn0038934 . [Q9VD44-1 ]
GeneIDi 42602.
KEGGi dme:Dmel_CG5732.
UCSCi CG5732-RA. d. melanogaster. [Q9VD44-1 ]

Organism-specific databases

CTDi 42602.
FlyBasei FBgn0038934. Gld2.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
InParanoidi Q9VD44.
KOi K14079.
OMAi NRYWREF.
OrthoDBi EOG7DRJ2S.
PhylomeDBi Q9VD44.

Miscellaneous databases

GenomeRNAii 42602.
NextBioi 829642.

Gene expression databases

Bgeei Q9VD44.

Family and domain databases

InterProi IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Testis.
  4. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
    Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Testis.
  5. Cited for: FUNCTION, INTERACTION WITH FMR1 AND EIF-4E, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-995.

Entry informationi

Entry nameiGLD2A_DROME
AccessioniPrimary (citable) accession number: Q9VD44
Secondary accession number(s): B5RJK9, Q8MR77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3