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Q9VD44

- GLD2A_DROME

UniProt

Q9VD44 - GLD2A_DROME

Protein

Poly(A) RNA polymerase gld-2 homolog A

Gene

Gld2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 3 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory.1 Publication

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi993 – 9931Magnesium or manganese; catalyticBy similarity
    Metal bindingi995 – 9951Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB
    4. protein binding Source: UniProtKB
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. long-term memory Source: FlyBase
    2. mRNA processing Source: UniProtKB-KW
    3. RNA polyadenylation Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase gld-2 homolog A (EC:2.7.7.19)
    Short name:
    DmGLD2
    Gene namesi
    Name:Gld2
    ORF Names:CG5732
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0038934. Gld2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Found in neuronal cytoplasm, present in discrete neuronal particles associated with mRNA control, and in nuclear puncta.

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase
    2. neuron projection Source: FlyBase
    3. nucleoplasm Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi995 – 9951D → A: Destroys active site. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13641364Poly(A) RNA polymerase gld-2 homolog APRO_0000341557Add
    BLAST

    Proteomic databases

    PaxDbiQ9VD44.

    Expressioni

    Tissue specificityi

    Expressed in the brain.1 Publication

    Developmental stagei

    Expressed in larvae, pupae and adults.1 Publication

    Gene expression databases

    BgeeiQ9VD44.

    Interactioni

    Subunit structurei

    Interacts with Fmr1 and eIF-4E.1 Publication

    Protein-protein interaction databases

    BioGridi67563. 2 interactions.
    IntActiQ9VD44. 2 interactions.
    MINTiMINT-1691551.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9VD44.
    SMRiQ9VD44. Positions 941-1251.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1163 – 122462PAP-associatedAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi4 – 118Poly-Ala
    Compositional biasi15 – 206Poly-Ala
    Compositional biasi465 – 49127Ser-richAdd
    BLAST
    Compositional biasi599 – 64547Ala-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    InParanoidiQ9VD44.
    KOiK14079.
    OMAiNRYWREF.
    OrthoDBiEOG7DRJ2S.
    PhylomeDBiQ9VD44.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: Q9VD44-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTAMAMAAA ASSSAAAATA TTTAISNSTN TTASPATTNT INTTTKTITT     50
    SEVPLEPASN MAPKALEIED SGRNEPEDLE HGAAKPLEQR KTTVVHTCPR 100
    PPGGYKYSME FLYGIGSGMA GIPLNIPTPS SITPRTVRST APLLTTHMPL 150
    LTNMGVAPSR PSGIRYPGSA GGSNGTTVAA STTPSTTVTT SSGSPGSGAE 200
    AITSSVSSSG LPAAGLQAAQ FICQGYMPTG PQRRLWHAEN AVWQFDRNYP 250
    YNQAYSPPYG IPMMPVGFEH PYGQRIIYPG YYNQTPPGIN PAAVAGLTRT 300
    NRPVTQQPHI LTQPAVGTTE SSEEAPATLG NAPQVSSTWR YGRPGTHRGR 350
    HAVPTAAAPA LLHRDSKSFY NSIGSGMANG PRFKAPFVAN VRNFQAGAVA 400
    TVAAGGAATT AVVGTSAPAT GAASSSDQNV ATKRNHQGAA TQNNHRNRHN 450
    AKKGGKNSVG KELTSNSSES LSNSSSKSQL NKRPSSSSSI SPIKHPHRNY 500
    RNRMRYTATE PTEQKAATTT VPISNYQPPQ QSTSTVRRTS KFQGSNAYQA 550
    HTAAGRQQSR FYQSRHMDGY VFQSGHYMVY AAGAPPVGLR PGKSPVSEAT 600
    GAPPGAAAAT AAAATAVAAT AAAAEGGGAA EGGGAAAAPV TASSATLEGE 650
    QPLDSDFDQR QEFADLGLDP ANGGFSSDLE PNGIKQDTSE LDTHSCLISH 700
    PNSEVDGDDN QSLASFAPSV ESDDSDSELS DASVESVARD ILVSCLAVAT 750
    GAEEPDFSGP NLVPYGDMHY LKELDKKNPP TNGYRSHRPY HQSHYAYHSQ 800
    MSPRGLSCCG DMLNQHSEDL VFKLDQNQPD GIESGKNIFL REITEQPDNI 850
    SVASNLSCSP SASSSKSVLA PMASKSNITM PEENNDDDEL PLVVHNRYWR 900
    EFFGYTPADR FLLRAKFVEM RRPPKVMGCK NKWDPLSLSV WKKFLESQQT 950
    RHVYKIKMRL WRAIYTVAMK NYPRYGLYLV GSSISYFGSK CSDMDICMLA 1000
    CTNPNIDSRM EAVYHLHVMK ELLGRTNMFQ DFNLIEARVP ILRFTDRCHK 1050
    VEVDINFNNS VGIRNTHLLY CYSQLDWRVR PMALTVKQWA QYHNINNAKN 1100
    MTISSYSLML MVIHFLQVGA SPPVLPCLHN LYPEKFGLLQ PNDFGYVDMN 1150
    EVMAPYQSDN SQTLGDLLLS FLHYYSVFDY GKYAISIRVG GVLPIEVCRA 1200
    ATAPKNDIHQ WNELCIEEPF DQTNTARSVY DTDTFERIKT IFVASYRRLD 1250
    STRNLSAIFE DYDGPTILMQ QPSVDSEIEL YEGQHHRLLP NRGSSRSNSA 1300
    IPSPRPSILM VDKATTAIWD DINNKPDHPV LSHSNNYDAT NECTGNGSLM 1350
    GLKDNSVADK PPIA 1364

    Note: No experimental confirmation available.

    Length:1,364
    Mass (Da):147,592
    Last modified:July 5, 2004 - v3
    Checksum:i9BAFCAE8D75083CA
    GO
    Isoform B (identifier: Q9VD44-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         881-1364: PEENNDDDEL...NSVADKPPIA → VGELKNVLLN...LIVHFIRFTK

    Note: No experimental confirmation available.

    Show »
    Length:919
    Mass (Da):96,248
    Checksum:i35B7D4D987E439A2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti523 – 5231I → V in AAM52588. (PubMed:12537569)Curated
    Sequence conflicti523 – 5231I → V in ACH95257. 1 PublicationCurated
    Sequence conflicti591 – 5911P → A in AAM52588. (PubMed:12537569)Curated
    Sequence conflicti591 – 5911P → A in ACH95257. 1 PublicationCurated
    Sequence conflicti610 – 6101T → TAA in AAM52588. (PubMed:12537569)Curated
    Sequence conflicti610 – 6101T → TAA in ACH95257. 1 PublicationCurated
    Sequence conflicti636 – 6361A → T in AAM52588. (PubMed:12537569)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei881 – 1364484PEENN…KPPIA → VGELKNVLLNGQITKFISSK SYFFLSSVCLIVHFIRFTK in isoform B. 1 PublicationVSP_034331Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF55959.3.
    AY122076 mRNA. Translation: AAM52588.1.
    BT044483 mRNA. Translation: ACH95257.1.
    RefSeqiNP_001262829.1. NM_001275900.1. [Q9VD44-1]
    NP_651012.2. NM_142755.4. [Q9VD44-1]
    UniGeneiDm.33737.

    Genome annotation databases

    EnsemblMetazoaiFBtr0305038; FBpp0293575; FBgn0038934. [Q9VD44-1]
    FBtr0310204; FBpp0301888; FBgn0038934. [Q9VD44-1]
    GeneIDi42602.
    KEGGidme:Dmel_CG5732.
    UCSCiCG5732-RA. d. melanogaster. [Q9VD44-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF55959.3 .
    AY122076 mRNA. Translation: AAM52588.1 .
    BT044483 mRNA. Translation: ACH95257.1 .
    RefSeqi NP_001262829.1. NM_001275900.1. [Q9VD44-1 ]
    NP_651012.2. NM_142755.4. [Q9VD44-1 ]
    UniGenei Dm.33737.

    3D structure databases

    ProteinModelPortali Q9VD44.
    SMRi Q9VD44. Positions 941-1251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 67563. 2 interactions.
    IntActi Q9VD44. 2 interactions.
    MINTi MINT-1691551.

    Proteomic databases

    PaxDbi Q9VD44.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0305038 ; FBpp0293575 ; FBgn0038934 . [Q9VD44-1 ]
    FBtr0310204 ; FBpp0301888 ; FBgn0038934 . [Q9VD44-1 ]
    GeneIDi 42602.
    KEGGi dme:Dmel_CG5732.
    UCSCi CG5732-RA. d. melanogaster. [Q9VD44-1 ]

    Organism-specific databases

    CTDi 42602.
    FlyBasei FBgn0038934. Gld2.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    InParanoidi Q9VD44.
    KOi K14079.
    OMAi NRYWREF.
    OrthoDBi EOG7DRJ2S.
    PhylomeDBi Q9VD44.

    Miscellaneous databases

    GenomeRNAii 42602.
    NextBioi 829642.

    Gene expression databases

    Bgeei Q9VD44.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
      Strain: Berkeley.
      Tissue: Testis.
    4. Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: Berkeley.
      Tissue: Testis.
    5. Cited for: FUNCTION, INTERACTION WITH FMR1 AND EIF-4E, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-995.

    Entry informationi

    Entry nameiGLD2A_DROME
    AccessioniPrimary (citable) accession number: Q9VD44
    Secondary accession number(s): B5RJK9, Q8MR77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 85 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3