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Protein

Poly(A) RNA polymerase gld-2 homolog A

Gene

Gld2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi993Magnesium or manganese; catalyticBy similarity1
Metal bindingi995Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • long-term memory Source: FlyBase
  • mRNA processing Source: UniProtKB-KW
  • RNA polyadenylation Source: UniProtKB

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.19. 1994.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase gld-2 homolog A (EC:2.7.7.19)
Short name:
DmGLD2
Gene namesi
Name:Gld2
ORF Names:CG5732
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0038934. Gld2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi995D → A: Destroys active site. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003415571 – 1364Poly(A) RNA polymerase gld-2 homolog AAdd BLAST1364

Proteomic databases

PaxDbiQ9VD44.
PRIDEiQ9VD44.

Expressioni

Tissue specificityi

Expressed in the brain.1 Publication

Developmental stagei

Expressed in larvae, pupae and adults.1 Publication

Gene expression databases

BgeeiFBgn0038934.
ExpressionAtlasiQ9VD44. differential.
GenevisibleiQ9VD44. DM.

Interactioni

Subunit structurei

Interacts with Fmr1 and eIF-4E.1 Publication

Protein-protein interaction databases

BioGridi67563. 2 interactors.
IntActiQ9VD44. 3 interactors.
MINTiMINT-1691551.
STRINGi7227.FBpp0301888.

Structurei

3D structure databases

ProteinModelPortaliQ9VD44.
SMRiQ9VD44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1163 – 1224PAP-associatedAdd BLAST62

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi4 – 11Poly-Ala8
Compositional biasi15 – 20Poly-Ala6
Compositional biasi465 – 491Ser-richAdd BLAST27
Compositional biasi599 – 645Ala-richAdd BLAST47

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2277. Eukaryota.
COG5260. LUCA.
GeneTreeiENSGT00550000074490.
InParanoidiQ9VD44.
KOiK14079.
OMAiLVPYGDM.
OrthoDBiEOG091G0BUD.
PhylomeDBiQ9VD44.

Family and domain databases

InterProiView protein in InterPro
IPR002058. PAP_assoc.
PfamiView protein in Pfam
PF03828. PAP_assoc. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q9VD44-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTAMAMAAA ASSSAAAATA TTTAISNSTN TTASPATTNT INTTTKTITT
60 70 80 90 100
SEVPLEPASN MAPKALEIED SGRNEPEDLE HGAAKPLEQR KTTVVHTCPR
110 120 130 140 150
PPGGYKYSME FLYGIGSGMA GIPLNIPTPS SITPRTVRST APLLTTHMPL
160 170 180 190 200
LTNMGVAPSR PSGIRYPGSA GGSNGTTVAA STTPSTTVTT SSGSPGSGAE
210 220 230 240 250
AITSSVSSSG LPAAGLQAAQ FICQGYMPTG PQRRLWHAEN AVWQFDRNYP
260 270 280 290 300
YNQAYSPPYG IPMMPVGFEH PYGQRIIYPG YYNQTPPGIN PAAVAGLTRT
310 320 330 340 350
NRPVTQQPHI LTQPAVGTTE SSEEAPATLG NAPQVSSTWR YGRPGTHRGR
360 370 380 390 400
HAVPTAAAPA LLHRDSKSFY NSIGSGMANG PRFKAPFVAN VRNFQAGAVA
410 420 430 440 450
TVAAGGAATT AVVGTSAPAT GAASSSDQNV ATKRNHQGAA TQNNHRNRHN
460 470 480 490 500
AKKGGKNSVG KELTSNSSES LSNSSSKSQL NKRPSSSSSI SPIKHPHRNY
510 520 530 540 550
RNRMRYTATE PTEQKAATTT VPISNYQPPQ QSTSTVRRTS KFQGSNAYQA
560 570 580 590 600
HTAAGRQQSR FYQSRHMDGY VFQSGHYMVY AAGAPPVGLR PGKSPVSEAT
610 620 630 640 650
GAPPGAAAAT AAAATAVAAT AAAAEGGGAA EGGGAAAAPV TASSATLEGE
660 670 680 690 700
QPLDSDFDQR QEFADLGLDP ANGGFSSDLE PNGIKQDTSE LDTHSCLISH
710 720 730 740 750
PNSEVDGDDN QSLASFAPSV ESDDSDSELS DASVESVARD ILVSCLAVAT
760 770 780 790 800
GAEEPDFSGP NLVPYGDMHY LKELDKKNPP TNGYRSHRPY HQSHYAYHSQ
810 820 830 840 850
MSPRGLSCCG DMLNQHSEDL VFKLDQNQPD GIESGKNIFL REITEQPDNI
860 870 880 890 900
SVASNLSCSP SASSSKSVLA PMASKSNITM PEENNDDDEL PLVVHNRYWR
910 920 930 940 950
EFFGYTPADR FLLRAKFVEM RRPPKVMGCK NKWDPLSLSV WKKFLESQQT
960 970 980 990 1000
RHVYKIKMRL WRAIYTVAMK NYPRYGLYLV GSSISYFGSK CSDMDICMLA
1010 1020 1030 1040 1050
CTNPNIDSRM EAVYHLHVMK ELLGRTNMFQ DFNLIEARVP ILRFTDRCHK
1060 1070 1080 1090 1100
VEVDINFNNS VGIRNTHLLY CYSQLDWRVR PMALTVKQWA QYHNINNAKN
1110 1120 1130 1140 1150
MTISSYSLML MVIHFLQVGA SPPVLPCLHN LYPEKFGLLQ PNDFGYVDMN
1160 1170 1180 1190 1200
EVMAPYQSDN SQTLGDLLLS FLHYYSVFDY GKYAISIRVG GVLPIEVCRA
1210 1220 1230 1240 1250
ATAPKNDIHQ WNELCIEEPF DQTNTARSVY DTDTFERIKT IFVASYRRLD
1260 1270 1280 1290 1300
STRNLSAIFE DYDGPTILMQ QPSVDSEIEL YEGQHHRLLP NRGSSRSNSA
1310 1320 1330 1340 1350
IPSPRPSILM VDKATTAIWD DINNKPDHPV LSHSNNYDAT NECTGNGSLM
1360
GLKDNSVADK PPIA
Note: No experimental confirmation available.
Length:1,364
Mass (Da):147,592
Last modified:July 5, 2004 - v3
Checksum:i9BAFCAE8D75083CA
GO
Isoform B (identifier: Q9VD44-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     881-1364: PEENNDDDEL...NSVADKPPIA → VGELKNVLLN...LIVHFIRFTK

Note: No experimental confirmation available.
Show »
Length:919
Mass (Da):96,248
Checksum:i35B7D4D987E439A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti523I → V in AAM52588 (PubMed:12537569).Curated1
Sequence conflicti523I → V in ACH95257 (Ref. 4) Curated1
Sequence conflicti591P → A in AAM52588 (PubMed:12537569).Curated1
Sequence conflicti591P → A in ACH95257 (Ref. 4) Curated1
Sequence conflicti610T → TAA in AAM52588 (PubMed:12537569).Curated1
Sequence conflicti610T → TAA in ACH95257 (Ref. 4) Curated1
Sequence conflicti636A → T in AAM52588 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034331881 – 1364PEENN…KPPIA → VGELKNVLLNGQITKFISSK SYFFLSSVCLIVHFIRFTK in isoform B. 1 PublicationAdd BLAST484

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF55959.3.
AY122076 mRNA. Translation: AAM52588.1.
BT044483 mRNA. Translation: ACH95257.1.
RefSeqiNP_001262829.1. NM_001275900.1. [Q9VD44-1]
NP_651012.2. NM_142755.4. [Q9VD44-1]
UniGeneiDm.33737.

Genome annotation databases

EnsemblMetazoaiFBtr0305038; FBpp0293575; FBgn0038934. [Q9VD44-1]
FBtr0310204; FBpp0301888; FBgn0038934. [Q9VD44-1]
GeneIDi42602.
KEGGidme:Dmel_CG5732.
UCSCiCG5732-RA. d. melanogaster. [Q9VD44-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLD2A_DROME
AccessioniPrimary (citable) accession number: Q9VD44
Secondary accession number(s): B5RJK9, Q8MR77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: August 30, 2017
This is version 107 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families