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Q9VD44 (GLD2A_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) RNA polymerase gld-2 homolog A

Short name=DmGLD2
EC=2.7.7.19
Gene names
Name:Gld2
ORF Names:CG5732
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1364 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Required for formation of long term memory. Ref.5

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Magnesium or manganese By similarity.

Subunit structure

Interacts with Fmr1 and eIF-4E. Ref.5

Subcellular location

Cytoplasm. Nucleus. Note: Found in neuronal cytoplasm, present in discrete neuronal particles associated with mRNA control, and in nuclear puncta. Ref.5

Tissue specificity

Expressed in the brain. Ref.5

Developmental stage

Expressed in larvae, pupae and adults. Ref.5

Sequence similarities

Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.

Contains 1 PAP-associated domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9VD44-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: Q9VD44-2)

The sequence of this isoform differs from the canonical sequence as follows:
     881-1364: PEENNDDDEL...NSVADKPPIA → VGELKNVLLN...LIVHFIRFTK
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13641364Poly(A) RNA polymerase gld-2 homolog A
PRO_0000341557

Regions

Domain1163 – 122462PAP-associated
Compositional bias4 – 118Poly-Ala
Compositional bias15 – 206Poly-Ala
Compositional bias465 – 49127Ser-rich
Compositional bias599 – 64547Ala-rich

Sites

Metal binding9931Magnesium or manganese; catalytic By similarity
Metal binding9951Magnesium or manganese; catalytic By similarity

Natural variations

Alternative sequence881 – 1364484PEENN…KPPIA → VGELKNVLLNGQITKFISSK SYFFLSSVCLIVHFIRFTK in isoform B.
VSP_034331

Experimental info

Mutagenesis9951D → A: Destroys active site. Ref.5
Sequence conflict5231I → V in AAM52588. Ref.3
Sequence conflict5231I → V in ACH95257. Ref.4
Sequence conflict5911P → A in AAM52588. Ref.3
Sequence conflict5911P → A in ACH95257. Ref.4
Sequence conflict6101T → TAA in AAM52588. Ref.3
Sequence conflict6101T → TAA in ACH95257. Ref.4
Sequence conflict6361A → T in AAM52588. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified July 5, 2004. Version 3.
Checksum: 9BAFCAE8D75083CA

FASTA1,364147,592
        10         20         30         40         50         60 
MSTAMAMAAA ASSSAAAATA TTTAISNSTN TTASPATTNT INTTTKTITT SEVPLEPASN 

        70         80         90        100        110        120 
MAPKALEIED SGRNEPEDLE HGAAKPLEQR KTTVVHTCPR PPGGYKYSME FLYGIGSGMA 

       130        140        150        160        170        180 
GIPLNIPTPS SITPRTVRST APLLTTHMPL LTNMGVAPSR PSGIRYPGSA GGSNGTTVAA 

       190        200        210        220        230        240 
STTPSTTVTT SSGSPGSGAE AITSSVSSSG LPAAGLQAAQ FICQGYMPTG PQRRLWHAEN 

       250        260        270        280        290        300 
AVWQFDRNYP YNQAYSPPYG IPMMPVGFEH PYGQRIIYPG YYNQTPPGIN PAAVAGLTRT 

       310        320        330        340        350        360 
NRPVTQQPHI LTQPAVGTTE SSEEAPATLG NAPQVSSTWR YGRPGTHRGR HAVPTAAAPA 

       370        380        390        400        410        420 
LLHRDSKSFY NSIGSGMANG PRFKAPFVAN VRNFQAGAVA TVAAGGAATT AVVGTSAPAT 

       430        440        450        460        470        480 
GAASSSDQNV ATKRNHQGAA TQNNHRNRHN AKKGGKNSVG KELTSNSSES LSNSSSKSQL 

       490        500        510        520        530        540 
NKRPSSSSSI SPIKHPHRNY RNRMRYTATE PTEQKAATTT VPISNYQPPQ QSTSTVRRTS 

       550        560        570        580        590        600 
KFQGSNAYQA HTAAGRQQSR FYQSRHMDGY VFQSGHYMVY AAGAPPVGLR PGKSPVSEAT 

       610        620        630        640        650        660 
GAPPGAAAAT AAAATAVAAT AAAAEGGGAA EGGGAAAAPV TASSATLEGE QPLDSDFDQR 

       670        680        690        700        710        720 
QEFADLGLDP ANGGFSSDLE PNGIKQDTSE LDTHSCLISH PNSEVDGDDN QSLASFAPSV 

       730        740        750        760        770        780 
ESDDSDSELS DASVESVARD ILVSCLAVAT GAEEPDFSGP NLVPYGDMHY LKELDKKNPP 

       790        800        810        820        830        840 
TNGYRSHRPY HQSHYAYHSQ MSPRGLSCCG DMLNQHSEDL VFKLDQNQPD GIESGKNIFL 

       850        860        870        880        890        900 
REITEQPDNI SVASNLSCSP SASSSKSVLA PMASKSNITM PEENNDDDEL PLVVHNRYWR 

       910        920        930        940        950        960 
EFFGYTPADR FLLRAKFVEM RRPPKVMGCK NKWDPLSLSV WKKFLESQQT RHVYKIKMRL 

       970        980        990       1000       1010       1020 
WRAIYTVAMK NYPRYGLYLV GSSISYFGSK CSDMDICMLA CTNPNIDSRM EAVYHLHVMK 

      1030       1040       1050       1060       1070       1080 
ELLGRTNMFQ DFNLIEARVP ILRFTDRCHK VEVDINFNNS VGIRNTHLLY CYSQLDWRVR 

      1090       1100       1110       1120       1130       1140 
PMALTVKQWA QYHNINNAKN MTISSYSLML MVIHFLQVGA SPPVLPCLHN LYPEKFGLLQ 

      1150       1160       1170       1180       1190       1200 
PNDFGYVDMN EVMAPYQSDN SQTLGDLLLS FLHYYSVFDY GKYAISIRVG GVLPIEVCRA 

      1210       1220       1230       1240       1250       1260 
ATAPKNDIHQ WNELCIEEPF DQTNTARSVY DTDTFERIKT IFVASYRRLD STRNLSAIFE 

      1270       1280       1290       1300       1310       1320 
DYDGPTILMQ QPSVDSEIEL YEGQHHRLLP NRGSSRSNSA IPSPRPSILM VDKATTAIWD 

      1330       1340       1350       1360 
DINNKPDHPV LSHSNNYDAT NECTGNGSLM GLKDNSVADK PPIA 

« Hide

Isoform B [UniParc].

Checksum: 35B7D4D987E439A2
Show »

FASTA91996,248

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Strain: Berkeley.
Tissue: Testis.
[4]Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: Berkeley.
Tissue: Testis.
[5]"GLD2 poly(A) polymerase is required for long-term memory."
Kwak J.E., Drier E., Barbee S.A., Ramaswami M., Yin J.C.P., Wickens M.
Proc. Natl. Acad. Sci. U.S.A. 105:14644-14649(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH FMR1 AND EIF-4E, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-995.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014297 Genomic DNA. Translation: AAF55959.3.
AY122076 mRNA. Translation: AAM52588.1.
BT044483 mRNA. Translation: ACH95257.1.
RefSeqNP_001262829.1. NM_001275900.1.
NP_651012.2. NM_142755.4.
UniGeneDm.33737.

3D structure databases

ProteinModelPortalQ9VD44.
SMRQ9VD44. Positions 941-1251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid67563. 2 interactions.
IntActQ9VD44. 2 interactions.
MINTMINT-1691551.

Proteomic databases

PaxDbQ9VD44.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0305038; FBpp0293575; FBgn0038934.
FBtr0310204; FBpp0301888; FBgn0038934.
GeneID42602.
KEGGdme:Dmel_CG5732.
UCSCCG5732-RA. d. melanogaster. [Q9VD44-1]

Organism-specific databases

CTD42602.
FlyBaseFBgn0038934. Gld2.

Phylogenomic databases

eggNOGCOG5260.
GeneTreeENSGT00550000074490.
InParanoidQ9VD44.
KOK14079.
OMANRYWREF.
OrthoDBEOG7DRJ2S.
PhylomeDBQ9VD44.

Gene expression databases

BgeeQ9VD44.

Family and domain databases

InterProIPR002058. PAP_assoc.
[Graphical view]
PfamPF03828. PAP_assoc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi42602.
NextBio829642.

Entry information

Entry nameGLD2A_DROME
AccessionPrimary (citable) accession number: Q9VD44
Secondary accession number(s): B5RJK9, Q8MR77
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: March 19, 2014
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase