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Protein

Endoribonuclease Dcr-1

Gene

Dcr-1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for RNA interference (RNAi); double-stranded RNA (dsRNA) induces potent and specific gene silencing. RNAi is mediated by the RNA-induced silencing complex (RISC), a sequence-specific, multicomponent nuclease that destroys or silences messenger RNAs homologous to the silencing trigger. May carry out the initiation step of RNAi by cleaving dsRNA to produce 22 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs. During embryogenesis, involved in germline fate determination.3 Publications

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi2032Magnesium or manganeseBy similarity1
Sitei2132Important for activityBy similarity1
Metal bindingi2136Magnesium or manganeseBy similarity1
Metal bindingi2139Magnesium or manganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • bidentate ribonuclease III activity Source: FlyBase
  • deoxyribonuclease I activity Source: GO_Central
  • double-stranded RNA binding Source: FlyBase
  • helicase activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • pre-miRNA binding Source: FlyBase
  • ribonuclease III activity Source: MGI
  • single-stranded RNA binding Source: FlyBase

GO - Biological processi

  • apoptotic DNA fragmentation Source: GO_Central
  • dendrite morphogenesis Source: FlyBase
  • dsRNA transport Source: FlyBase
  • germarium-derived female germ-line cyst formation Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • germ-line stem cell division Source: FlyBase
  • mitotic cell cycle, embryonic Source: FlyBase
  • pole cell formation Source: FlyBase
  • pre-miRNA processing Source: FlyBase
  • production of miRNAs involved in gene silencing by miRNA Source: FlyBase
  • production of siRNA involved in RNA interference Source: UniProtKB
  • response to starvation Source: FlyBase
  • RNA interference Source: FlyBase
  • segment polarity determination Source: FlyBase
  • siRNA loading onto RISC involved in RNA interference Source: FlyBase

Keywordsi

Molecular functionEndonuclease, Helicase, Hydrolase, Nuclease, RNA-binding
Biological processRNA-mediated gene silencing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-203927 MicroRNA (miRNA) biogenesis
R-DME-426486 Small interfering RNA (siRNA) biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Endoribonuclease Dcr-1 (EC:3.1.26.-)
Short name:
Protein dicer-1
Gene namesi
Name:Dcr-1Imported
ORF Names:CG4792
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039016 Dcr-1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001804741 – 2249Endoribonuclease Dcr-1Add BLAST2249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1423Phosphoserine1 Publication1
Modified residuei1877Phosphoserine1 Publication1
Modified residuei1880Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VCU9
PRIDEiQ9VCU9

PTM databases

iPTMnetiQ9VCU9

Expressioni

Gene expression databases

BgeeiFBgn0039016
ExpressionAtlasiQ9VCU9 differential
GenevisibleiQ9VCU9 DM

Interactioni

Subunit structurei

Interacts with AGO2 to form components of the RISC. Interacts with Fmr1 to form a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are mRpL5, mRpL11, AGO2 and Rm62. Interacts with piwi and vas; these interactions occur in the polar granules.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi67642, 24 interactors
DIPiDIP-23028N
IntActiQ9VCU9, 13 interactors
STRINGi7227.FBpp0083717

Structurei

3D structure databases

ProteinModelPortaliQ9VCU9
SMRiQ9VCU9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini485 – 648Helicase C-terminalPROSITE-ProRule annotationAdd BLAST164
Domaini825 – 920Dicer dsRNA-binding foldPROSITE-ProRule annotationAdd BLAST96
Domaini1099 – 1246PAZPROSITE-ProRule annotationAdd BLAST148
Domaini1698 – 1919RNase III 1PROSITE-ProRule annotationAdd BLAST222
Domaini1993 – 2150RNase III 2PROSITE-ProRule annotationAdd BLAST158
Domaini2175 – 2241DRBMPROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Belongs to the helicase family. Dicer subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0701 Eukaryota
COG0571 LUCA
GeneTreeiENSGT00510000046789
InParanoidiQ9VCU9
KOiK11592
OMAiCYPKAIP
OrthoDBiEOG091G00M8
PhylomeDBiQ9VCU9

Family and domain databases

CDDicd00079 HELICc, 1 hit
cd00593 RIBOc, 2 hits
Gene3Di1.10.1520.10, 1 hit
3.30.160.380, 1 hit
InterProiView protein in InterPro
IPR038248 Dicer_dimer_sf
IPR005034 Dicer_dimerisation_dom
IPR014720 dsRBD_dom
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR003100 PAZ_dom
IPR036085 PAZ_dom_sf
IPR000999 RNase_III_dom
IPR036389 RNase_III_sf
PfamiView protein in Pfam
PF03368 Dicer_dimer, 1 hit
PF00271 Helicase_C, 1 hit
PF02170 PAZ, 1 hit
PF00636 Ribonuclease_3, 2 hits
SMARTiView protein in SMART
SM00490 HELICc, 1 hit
SM00949 PAZ, 1 hit
SM00535 RIBOc, 2 hits
SUPFAMiSSF101690 SSF101690, 2 hits
SSF52540 SSF52540, 2 hits
SSF69065 SSF69065, 4 hits
PROSITEiView protein in PROSITE
PS51327 DICER_DSRBF, 1 hit
PS50137 DS_RBD, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS50821 PAZ, 1 hit
PS00517 RNASE_3_1, 1 hit
PS50142 RNASE_3_2, 2 hits

Sequencei

Sequence statusi: Complete.

Q9VCU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFHWCDNNL HTTVFTPRDF QVELLATAYE RNTIICLGHR SSKEFIALKL
60 70 80 90 100
LQELSRRARR HGRVSVYLSC EVGTSTEPCS IYTMLTHLTD LRVWQEQPDM
110 120 130 140 150
QIPFDHCWTD YHVSILRPEG FLYLLETREL LLSSVELIVL EDCHDSAVYQ
160 170 180 190 200
RIRPLFENHI MPAPPADRPR ILGLAGPLHS AGCELQQLSA MLATLEQSVL
210 220 230 240 250
CQIETASDIV TVLRYCSRPH EYIVQCAPFE MDELSLVLAD VLNTHKSFLL
260 270 280 290 300
DHRYDPYEIY GTDQFMDELK DIPDPKVDPL NVINSLLVVL HEMGPWCTQR
310 320 330 340 350
AAHHFYQCNE KLKVKTPHER HYLLYCLVST ALIQLYSLCE HAFHRHLGSG
360 370 380 390 400
SDSRQTIERY SSPKVRRLLQ TLRCFKPEEV HTQADGLRRM RHQVDQADFN
410 420 430 440 450
RLSHTLESKC RMVDQMDQPP TETRALVATL EQILHTTEDR QTNRSAARVT
460 470 480 490 500
PTPTPAHAKP KPSSGANTAQ PRTRRRVYTR RHHRDHNDGS DTLCALIYCN
510 520 530 540 550
QNHTARVLFE LLAEISRRDP DLKFLRCQYT TDRVADPTTE PKEAELEHRR
560 570 580 590 600
QEEVLKRFRM HDCNVLIGTS VLEEGIDVPK CNLVVRWDPP TTYRSYVQCK
610 620 630 640 650
GRARAAPAYH VILVAPSYKS PTVGSVQLTD RSHRYICATG DTTEADSDSD
660 670 680 690 700
DSAMPNSSGS DPYTFGTARG TVKILNPEVF SKQPPTACDI KLQEIQDELP
710 720 730 740 750
AAAQLDTSNS SDEAVSMSNT SPSESSTEQK SRRFQCELSS LTEPEDTSDT
760 770 780 790 800
TAEIDTAHSL ASTTKDLVHQ MAQYREIEQM LLSKCANTEP PEQEQSEAER
810 820 830 840 850
FSACLAAYRP KPHLLTGASV DLGSAIALVN KYCARLPSDT FTKLTALWRC
860 870 880 890 900
TRNERAGVTL FQYTLRLPIN SPLKHDIVGL PMPTQTLARR LAALQACVEL
910 920 930 940 950
HRIGELDDQL QPIGKEGFRA LEPDWECFEL EPEDEQIVQL SDEPRPGTTK
960 970 980 990 1000
RRQYYYKRIA SEFCDCRPVA GAPCYLYFIQ LTLQCPIPEE QNTRGRKIYP
1010 1020 1030 1040 1050
PEDAQQGFGI LTTKRIPKLS AFSIFTRSGE VKVSLELAKE RVILTSEQIV
1060 1070 1080 1090 1100
CINGFLNYTF TNVLRLQKFL MLFDPDSTEN CVFIVPTVKA PAGGKHIDWQ
1110 1120 1130 1140 1150
FLELIQANGN TMPRAVPDEE RQAQPFDPQR FQDAVVMPWY RNQDQPQYFY
1160 1170 1180 1190 1200
VAEICPHLSP LSCFPGDNYR TFKHYYLVKY GLTIQNTSQP LLDVDHTSAR
1210 1220 1230 1240 1250
LNFLTPRYVN RKGVALPTSS EETKRAKREN LEQKQILVPE LCTVHPFPAS
1260 1270 1280 1290 1300
LWRTAVCLPC ILYRINGLLL ADDIRKQVSA DLGLGRQQIE DEDFEWPMLD
1310 1320 1330 1340 1350
FGWSLSEVLK KSRESKQKES LKDDTINGKD LADVEKKPTS EETQLDKDSK
1360 1370 1380 1390 1400
DDKVEKSAIE LIIEGEEKLQ EADDFIEIGT WSNDMADDIA SFNQEDDDED
1410 1420 1430 1440 1450
DAFHLPVLPA NVKFCDQQTR YGSPTFWDVS NGESGFKGPK SSQNKQGGKG
1460 1470 1480 1490 1500
KAKGPAKPTF NYYDSDNSLG SSYDDDDNAG PLNYMHHNYS SDDDDVADDI
1510 1520 1530 1540 1550
DAGRIAFTSK NEAETIETAQ EVEKRQKQLS IIQATNANER QYQQTKNLLI
1560 1570 1580 1590 1600
GFNFKHEDQK EPATIRYEES IAKLKTEIES GGMLVPHDQQ LVLKRSDAAE
1610 1620 1630 1640 1650
AQVAKVSMME LLKQLLPYVN EDVLAKKLGD RRELLLSDLV ELNADWVARH
1660 1670 1680 1690 1700
EQETYNVMGC GDSFDNYNDH HRLNLDEKQL KLQYERIEIE PPTSTKAITS
1710 1720 1730 1740 1750
AILPAGFSFD RQPDLVGHPG PSPSIILQAL TMSNANDGIN LERLETIGDS
1760 1770 1780 1790 1800
FLKYAITTYL YITYENVHEG KLSHLRSKQV ANLNLYRLGR RKRLGEYMIA
1810 1820 1830 1840 1850
TKFEPHDNWL PPCYYVPKEL EKALIEAKIP THHWKLADLL DIKNLSSVQI
1860 1870 1880 1890 1900
CEMVREKADA LGLEQNGGAQ NGQLDDSNDS CNDFSCFIPY NLVSQHSIPD
1910 1920 1930 1940 1950
KSIADCVEAL IGAYLIECGP RGALLFMAWL GVRVLPITRQ LDGGNQEQRI
1960 1970 1980 1990 2000
PGSTKPNAEN VVTVYGAWPT PRSPLLHFAP NATEELDQLL SGFEEFEESL
2010 2020 2030 2040 2050
GYKFRDRSYL LQAMTHASYT PNRLTDCYQR LEFLGDAVLD YLITRHLYED
2060 2070 2080 2090 2100
PRQHSPGALT DLRSALVNNT IFASLAVRHG FHKFFRHLSP GLNDVIDRFV
2110 2120 2130 2140 2150
RIQQENGHCI SEEYYLLSEE ECDDAEDVEV PKALGDVFES IAGAIFLDSN
2160 2170 2180 2190 2200
MSLDVVWHVY SNMMSPEIEQ FSNSVPKSPI RELLELEPET AKFGKPEKLA
2210 2220 2230 2240
DGRRVRVTVD VFCKGTFRGI GRNYRIAKCT AAKCALRQLK KQGLIAKKD
Length:2,249
Mass (Da):255,330
Last modified:May 1, 2000 - v1
Checksum:iD693F0432AC8033D
GO

Sequence cautioni

The sequence AAK84929 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1338 – 1339PT → AI (PubMed:12537569).Curated2
Sequence conflicti1345L → I (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA Translation: AAF56056.1
AY050230 mRNA Translation: AAK84929.1 Different initiation.
RefSeqiNP_524453.1, NM_079729.3
UniGeneiDm.1283

Genome annotation databases

EnsemblMetazoaiFBtr0084324; FBpp0083717; FBgn0039016
GeneIDi42693
KEGGidme:Dmel_CG4792
UCSCiCG4792-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiDCR1_DROME
AccessioniPrimary (citable) accession number: Q9VCU9
Secondary accession number(s): Q961S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health