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Protein

Probable N6-adenosine-methyltransferase MT-A70-like protein

Gene

Ime4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Probable N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites in mRNA isolated, may play a role in the efficiency of mRNA splicing, transport or translation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am.

GO - Molecular functioni

  1. mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity Source: UniProtKB
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. germarium-derived female germ-line cyst encapsulation Source: FlyBase
  2. mRNA methylation Source: GOC
  3. ovarian follicle cell development Source: FlyBase
  4. regulation of gene silencing Source: FlyBase
  5. regulation of Notch signaling pathway Source: FlyBase
  6. RNA methylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_207945. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable N6-adenosine-methyltransferase MT-A70-like protein (EC:2.1.1.62)
Alternative name(s):
Inducer of meiosis 4
Gene namesi
Name:Ime4
ORF Names:CG5933
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039139. Ime4.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Probable N6-adenosine-methyltransferase MT-A70-like proteinPRO_0000207633Add
BLAST

Proteomic databases

PaxDbiQ9VCE6.
PRIDEiQ9VCE6.

Expressioni

Gene expression databases

BgeeiQ9VCE6.

Interactioni

Protein-protein interaction databases

BioGridi67779. 16 interactions.
DIPiDIP-17384N.
IntActiQ9VCE6. 4 interactions.
MINTiMINT-308015.
STRINGi7227.FBpp0083917.

Structurei

3D structure databases

ProteinModelPortaliQ9VCE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MT-A70-like family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4725.
GeneTreeiENSGT00550000075058.
InParanoidiQ9VCE6.
KOiK05925.
OMAiCDLRFLD.
OrthoDBiEOG7PZRWV.
PhylomeDBiQ9VCE6.

Family and domain databases

InterProiIPR025848. MT-A70.
IPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VCE6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADAWDIKSL KTKRNTLREK LEKRKKERIE ILSDIQEDLT NPKKELVEAD
60 70 80 90 100
LEVQKEVLQA LSSCSLALPI VSTQVVEKIA GSSLEMVNFI LGKLANQGAI
110 120 130 140 150
VIRNVTIGTE AGCEIISVQP KELKEILEDT NDTCQQKEEE AKRKLEVDDV
160 170 180 190 200
DQPQEKTIKL ESTVARKEST SLDAPDDIMM LLSMPSTREK QSKQVGEEIL
210 220 230 240 250
ELLTKPTAKE RSVAEKFKSH GGAQVMEFCS HGTKVECLKA QQATAEMAAK
260 270 280 290 300
KKQERRDEKE LRPDVDAGEN VTGKVPKTES AAEDGEIIAE VINNCEAESQ
310 320 330 340 350
ESTDGSDTCS SETTDKCTKL HFKKIIQAHT DESLGDCSFL NTCFHMATCK
360 370 380 390 400
YVHYEVDTLP HINTNKPTDV KTKLSLKRSV DSSCTLYPPQ WIQCDLRFLD
410 420 430 440 450
MTVLGKFAVV MADPPWDIHM ELPYGTMSDD EMRALGVPAL QDDGLIFLWV
460 470 480 490 500
TGRAMELGRD CLKLWGYERV DELIWVKTNQ LQRIIRTGRT GHWLNHGKEH
510 520 530 540 550
CLVGMKGNPT NLNRGLDCDV IVAEVRATSH KPDEIYGIIE RLSPGTRKIE
560 570 580 590 600
LFGRPHNIQP NWITLGNQLD GIRLVDPELI TQFQKRYPDG NCMSPASANA

ASINGIQK
Length:608
Mass (Da):68,101
Last modified:May 1, 2000 - v1
Checksum:i00A2E06082E7FD2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56221.1.
AY113286 mRNA. Translation: AAM29291.1.
RefSeqiNP_651204.1. NM_142947.4.
UniGeneiDm.13317.

Genome annotation databases

EnsemblMetazoaiFBtr0084532; FBpp0083917; FBgn0039139.
GeneIDi42844.
KEGGidme:Dmel_CG5933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56221.1.
AY113286 mRNA. Translation: AAM29291.1.
RefSeqiNP_651204.1. NM_142947.4.
UniGeneiDm.13317.

3D structure databases

ProteinModelPortaliQ9VCE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67779. 16 interactions.
DIPiDIP-17384N.
IntActiQ9VCE6. 4 interactions.
MINTiMINT-308015.
STRINGi7227.FBpp0083917.

Proteomic databases

PaxDbiQ9VCE6.
PRIDEiQ9VCE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0084532; FBpp0083917; FBgn0039139.
GeneIDi42844.
KEGGidme:Dmel_CG5933.

Organism-specific databases

CTDi42844.
FlyBaseiFBgn0039139. Ime4.

Phylogenomic databases

eggNOGiCOG4725.
GeneTreeiENSGT00550000075058.
InParanoidiQ9VCE6.
KOiK05925.
OMAiCDLRFLD.
OrthoDBiEOG7PZRWV.
PhylomeDBiQ9VCE6.

Enzyme and pathway databases

ReactomeiREACT_207945. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

GenomeRNAii42844.
NextBioi830874.
PROiQ9VCE6.

Gene expression databases

BgeeiQ9VCE6.

Family and domain databases

InterProiIPR025848. MT-A70.
IPR007757. MT-A70-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF05063. MT-A70. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51143. MT_A70. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  2. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Testis.

Entry informationi

Entry nameiMTA70_DROME
AccessioniPrimary (citable) accession number: Q9VCE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.