Q9VBP9 (NPL4_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear protein localization protein 4 homolog

Gene names

ORF Names:

CG4673

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	652 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be part of a complex that binds ubiquitinated proteins and that is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome By similarity.
Pathway	Protein degradation; proteasomal ubiquitin-dependent pathway.
Sequence similarities	Belongs to the NPL4 family. Contains 1 RanBP2-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9VBP9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B (identifier: Q9VBP9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: MACAPLLLEQFIYKKRNFAYAFVRHRLFVLCKQS → MPNDKI

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 652	652	Nuclear protein localization protein 4 homolog		PRO_0000372853
Regions
	Zinc finger	625 – 652	28	RanBP2-type
Amino acid modifications
	Modified residue	114	1	Phosphothreonine Ref.6
	Modified residue	115	1	Phosphoserine Ref.5 Ref.6
	Modified residue	117	1	Phosphothreonine Ref.6
	Modified residue	138	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 34	34	MACAP…LCKQS → MPNDKI in isoform B.		VSP_037209

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified April 26, 2005. Version 3. Checksum: B8D3B986EE19786A	FASTA	652	73,361
	10 20 30 40 50 60 MACAPLLLEQ FIYKKRNFAY AFVRHRLFVL CKQSLIRVQS AEGIKRIEIS PKSNLKHLYD 70 80 90 100 110 120 SVQNALKVDG FGLFKERNFL TELQASGSQL VGTSLRHGDM VYLKQMAGTS SRRTSTTVLD 130 140 150 160 170 180 SQAFKTSTIS NPNSARPSFN VIEDDVDQAL SKADGTIKRE RDSKLCHHNA NGRCVHCSAL 190 200 210 220 230 240 EPYDESYLKE HNIKHLSFHS YIRKQTSGMD QGKYFVFDDI NCRIKPGCRE HPPWPKGICS 250 260 270 280 290 300 KCQPSAITLN RQTYRHVDNV MFENTKIVER FLNYWRTTGH QRMGYLYGTY EQHTDVPLGI 310 320 330 340 350 360 RAKVAAIYEP PQESTRDSIN IQPDEFADDV DAVASALGLK KIGWIFTDLI TDDASIGTVK 370 380 390 400 410 420 QIRGIESHFI TAQECITAGE LQNRHPNPCK YASNGVFGSK FVTICVTGDK TKQVHMEGYA 430 440 450 460 470 480 VSAQCMALVR DNCLIPTKDA PELGYVREST DKQYVPDVFY KEKDLYGNEV QRLARPLPVE 490 500 510 520 530 540 YLLVDVPAST PLQPIYTFTE YDKRQPFPIE NRYIDGHLQD FNALSCYLSA WGEEEFLEAI 550 560 570 580 590 600 SDFHLLVYLY KMDMLPLRQH MGPLLEAVRT KNPNQAAQFK VVDVWKLLES LIQASSGGSG 610 620 630 640 650 GTTSYPSGGA SASAGASGSE AMDLDANTWT CNHCTFINRG ELTSCEICSL PR
	Isoform B [UniParc]. Checksum: 872438E83CB4ACAD	FASTA	624	69,972
	10 20 30 40 50 60 MPNDKILIRV QSAEGIKRIE ISPKSNLKHL YDSVQNALKV DGFGLFKERN FLTELQASGS 70 80 90 100 110 120 QLVGTSLRHG DMVYLKQMAG TSSRRTSTTV LDSQAFKTST ISNPNSARPS FNVIEDDVDQ 130 140 150 160 170 180 ALSKADGTIK RERDSKLCHH NANGRCVHCS ALEPYDESYL KEHNIKHLSF HSYIRKQTSG 190 200 210 220 230 240 MDQGKYFVFD DINCRIKPGC REHPPWPKGI CSKCQPSAIT LNRQTYRHVD NVMFENTKIV 250 260 270 280 290 300 ERFLNYWRTT GHQRMGYLYG TYEQHTDVPL GIRAKVAAIY EPPQESTRDS INIQPDEFAD 310 320 330 340 350 360 DVDAVASALG LKKIGWIFTD LITDDASIGT VKQIRGIESH FITAQECITA GELQNRHPNP 370 380 390 400 410 420 CKYASNGVFG SKFVTICVTG DKTKQVHMEG YAVSAQCMAL VRDNCLIPTK DAPELGYVRE 430 440 450 460 470 480 STDKQYVPDV FYKEKDLYGN EVQRLARPLP VEYLLVDVPA STPLQPIYTF TEYDKRQPFP 490 500 510 520 530 540 IENRYIDGHL QDFNALSCYL SAWGEEEFLE AISDFHLLVY LYKMDMLPLR QHMGPLLEAV 550 560 570 580 590 600 RTKNPNQAAQ FKVVDVWKLL ESLIQASSGG SGGTTSYPSG GASASAGASG SEAMDLDANT 610 620 WTCNHCTFIN RGELTSCEIC SLPR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
[4]	"Identification and characterization of a Drosophila proteasome regulatory network." Lundgren J., Masson P., Mirzaei Z., Young P. Mol. Cell. Biol. 25:4662-4675(2005) [PubMed: 15899868] [Abstract] Cited for: IDENTIFICATION.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115 AND SER-138, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-114; SER-115 AND THR-117, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAF56480.3. AE014297 Genomic DNA. Translation: AAN14058.2. AY058286 mRNA. Translation: AAL13515.1.
RefSeq	NP_001163731.1. NM_001170260.1. NP_651407.3. NM_143150.4. NP_733110.2. NM_170231.2.
UniGene	Dm.4324.
3D structure databases
HSSP	HSSP built from PDB template 1Q5W based on UniProtKB Q9ES54.
ProteinModelPortal	Q9VBP9.
SMR	Q9VBP9. Positions 32-103, 626-652.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VBP9. 17 interactions.
MINT	MINT-1565061.
STRING	Q9VBP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0084893; FBpp0084267; FBgn0039348.
GeneID	43091.
KEGG	dme:Dmel_CG4673.
UCSC	CG4673-RA. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0039348. CG4673.
Phylogenomic databases
eggNOG	inNOG08956.
GeneTree	EMGT00050000008511.
InParanoid	Q9VBP9.
OMA	MCRLSPD.
OrthoDB	EOG4BG7B0.
PhylomeDB	Q9VBP9.
Gene expression databases
ArrayExpress	Q9VBP9.
Bgee	Q9VBP9.
Family and domain databases
InterPro	IPR007717. NPL4. IPR024682. Npl4_Ub-like_dom. IPR007716. NPL4_Zn-bd_put. IPR016563. PolyUb_recognition_cplx_Npl4. IPR001876. Znf_RanBP2. [Graphical view]
KO	K14015.
Pfam	PF05021. NPL4. 1 hit. PF11543. UN_NPL4. 1 hit. PF05020. zf-NPL4. 1 hit. [Graphical view]
PIRSF	PIRSF010052. Polyub_prc_Npl4. 1 hit.
SMART	SM00547. ZnF_RBZ. 1 hit. [Graphical view]
PROSITE	PS01358. ZF_RANBP2_1. 1 hit. PS50199. ZF_RANBP2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	832165.

Entry information

Entry name

NPL4_DROME

Accession

Primary (citable) accession number: Q9VBP9
Secondary accession number(s): Q8IMS6, Q95U63

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project