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Protein

E3 ubiquitin-protein ligase RING1

Gene

Sce

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-118' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-118' ubiquitination gives a specific tag for epigenetic transcriptional repression. Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. PcG complexes act via modification of histones, such as methylation, deacetylation, ubiquitination rendering chromatin heritably changed in its expressibility. May play a role in meiotic sister chromatid cohesion.4 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 86RING-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing Source: UniProtKB
  • germarium-derived female germ-line cyst encapsulation Source: FlyBase
  • histone H2A monoubiquitination Source: FlyBase
  • meiotic cell cycle Source: UniProtKB-KW
  • multicellular organism development Source: UniProtKB
  • neurogenesis Source: FlyBase
  • ovarian follicle cell stalk formation Source: FlyBase
  • sex comb development Source: UniProtKB
  • sister chromatid cohesion Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Repressor

Keywords - Biological processi

Cell cycle, Meiosis, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-4570464. SUMOylation of RNA binding proteins.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RING1 (EC:6.3.2.-)
Alternative name(s):
Sex comb extra protein
dRING protein
dRING1
Gene namesi
Name:Sce
ORF Names:CG5595
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003330. Sce.

Subcellular locationi

  • Nucleus
  • Chromosome

  • Note: Colocalizes with ubiquitinated histone H2A. Colocalizes with Pc, Pcl, Psc, ph at many sites on polytene chromosomes. Colocalizes with ORD on the chromatin of primary spermatocytes during G2 of meiosis.

GO - Cellular componenti

  • cohesin complex Source: UniProtKB
  • nucleolus Source: FlyBase
  • nucleus Source: FlyBase
  • PcG protein complex Source: FlyBase
  • PRC1 complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65R → C in Sce33M2; induces extra sex combs due to derepression of Ubx homeotic gene and abolishes ability to ubiquitinate histone H2A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561131 – 435E3 ubiquitin-protein ligase RING1Add BLAST435

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202Phosphoserine1 Publication1
Modified residuei266Phosphoserine1 Publication1
Modified residuei267Phosphothreonine1 Publication1
Modified residuei269Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9VB08.
PRIDEiQ9VB08.

PTM databases

iPTMnetiQ9VB08.

Expressioni

Tissue specificityi

Ubiquitously expressed in syncytial blastoderm embryos. Ubiquitously expressed until stage 11. Then, it is only expressed in the neuroectoderm. Later in embryonic development, it is only expressed in the CNS. In larvae, it is expressed in all imaginal disks. Expressed in the male and female gonads.2 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0003330.
GenevisibleiQ9VB08. DM.

Interactioni

Subunit structurei

Interacts with ORD. Component of PRC1 complex, which contains many PcG proteins like Pc, ph, Scm, Psc, Sce and also chromatin remodeling proteins such as histone deacetylases. This complex is distinct from the Esc/E(z) complex, at least composed of esc, E(z), Su(z)12, Rpd3 and Caf1. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing.4 Publications

Protein-protein interaction databases

BioGridi68209. 16 interactors.
DIPiDIP-21764N.
IntActiQ9VB08. 3 interactors.
MINTiMINT-860974.
STRINGi7227.FBpp0084614.

Structurei

3D structure databases

ProteinModelPortaliQ9VB08.
SMRiQ9VB08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi158 – 171Gly-richAdd BLAST14
Compositional biasi222 – 299Ser-richAdd BLAST78

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 86RING-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
InParanoidiQ9VB08.
KOiK10695.
OMAiHTIDKED.
OrthoDBiEOG091G06VO.
PhylomeDBiQ9VB08.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VB08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLDPAPNK TWELSLYELQ RKPQEVITDS TEIAVSPRSL HSELMCPICL
60 70 80 90 100
DMLKKTMTTK ECLHRFCSDC IVTALRSGNK ECPTCRKKLV SKRSLRADPN
110 120 130 140 150
FDLLISKIYP SREEYEAIQE KVMAKFNQTQ SQQALVNSIN EGIKLQSQNR
160 170 180 190 200
PQRFRTKGGG GGGGGGGNGN GAANVAAPPA PGAPTAVGRN ASNQMHVHDT
210 220 230 240 250
ASNDSNSNTN SIDRENRDPG HSGTSAASAI TSASNAAPSS SANSGASTSA
260 270 280 290 300
TRMQVDDASN PPSVRSTPSP VPSNSSSSKP KRAMSVLTSE RSEESESDSQ
310 320 330 340 350
MDCRTEGDSN IDTEGEGNGE LGINDEIELV FKPHPTEMSA DNQLIRALKE
360 370 380 390 400
NCVRYIKTTA NATVDHLSKY LAMRLTLDLG ADLPEACRVL NFCIYVAPQP
410 420 430
QQLVILNGNQ TLHQVNDKFW KVNKPMEMYY SWKKT
Length:435
Mass (Da):47,256
Last modified:May 1, 2000 - v1
Checksum:i5DC5834BD3CC516C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti176A → P in CAA04797 (Ref. 1) Curated1
Sequence conflicti201A → P in CAA04797 (Ref. 1) Curated1
Sequence conflicti279K → N in CAA04797 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001514 mRNA. Translation: CAA04797.1.
AE014297 Genomic DNA. Translation: AAF56737.1.
AY058535 mRNA. Translation: AAL13764.1.
RefSeqiNP_477509.1. NM_058161.4.
UniGeneiDm.4170.

Genome annotation databases

EnsemblMetazoaiFBtr0085245; FBpp0084614; FBgn0003330.
GeneIDi43327.
KEGGidme:Dmel_CG5595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001514 mRNA. Translation: CAA04797.1.
AE014297 Genomic DNA. Translation: AAF56737.1.
AY058535 mRNA. Translation: AAL13764.1.
RefSeqiNP_477509.1. NM_058161.4.
UniGeneiDm.4170.

3D structure databases

ProteinModelPortaliQ9VB08.
SMRiQ9VB08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68209. 16 interactors.
DIPiDIP-21764N.
IntActiQ9VB08. 3 interactors.
MINTiMINT-860974.
STRINGi7227.FBpp0084614.

PTM databases

iPTMnetiQ9VB08.

Proteomic databases

PaxDbiQ9VB08.
PRIDEiQ9VB08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085245; FBpp0084614; FBgn0003330.
GeneIDi43327.
KEGGidme:Dmel_CG5595.

Organism-specific databases

CTDi43327.
FlyBaseiFBgn0003330. Sce.

Phylogenomic databases

eggNOGiKOG0311. Eukaryota.
ENOG410XQ5G. LUCA.
GeneTreeiENSGT00390000016977.
InParanoidiQ9VB08.
KOiK10695.
OMAiHTIDKED.
OrthoDBiEOG091G06VO.
PhylomeDBiQ9VB08.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-2559580. Oxidative Stress Induced Senescence.
R-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

GenomeRNAii43327.
PROiQ9VB08.

Gene expression databases

BgeeiFBgn0003330.
GenevisibleiQ9VB08. DM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRING1_DROME
AccessioniPrimary (citable) accession number: Q9VB08
Secondary accession number(s): O18380
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.