Reviewed, UniProtKB/Swiss-Prot Q9VAW5 (LARP_DROME)

Names and origin

Protein names

Recommended name:
    La-related protein
Alternative name(s):
    dlarp

Gene names

Name:	larp
ORF Names:	CG14066

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cytoplasm. Ref.1 Ref.5
Developmental stage	At the end of embryogenesis, accumulates in the gut and muscles. Ref.1
Sequence similarities	Contains 1 HTH La-type RNA-binding domain.
Sequence caution	The sequence AAF35862.1 differs from that shown. Reason: Miscellaneous discrepancy. Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon. The sequence AAM29658.1 differs from that shown. Reason: Miscellaneous discrepancy. Unusual initiator. The initiator methionine is coded by a non-canonical ATA isoleucine codon.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ena	Q8T4F7	1	EBI-123668,EBI-466810

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform C (identifier: Q9VAW5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q9VAW5-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-438: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1403	1403	La-related protein		PRO_0000207613
Regions
	Domain	453 – 544	92	HTH La-type RNA-binding
	Compositional bias	26 – 31	6	Poly-Gln
	Compositional bias	52 – 59	8	Poly-Gln
	Compositional bias	62 – 69	8	Poly-Thr
	Compositional bias	70 – 84	15	Poly-Ala
	Compositional bias	102 – 110	9	Poly-Thr
	Compositional bias	210 – 217	8	Poly-Thr
	Compositional bias	599 – 604	6	Poly-Pro
	Compositional bias	715 – 718	4	Poly-Gln
	Compositional bias	726 – 731	6	Poly-Asn
	Compositional bias	772 – 785	14	Poly-Thr
	Compositional bias	951 – 955	5	Poly-Pro
	Compositional bias	1273 – 1283	11	Poly-Gln
	Compositional bias	1350 – 1353	4	Poly-Gln
	Compositional bias	1395 – 1401	7	Poly-Ser
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.7
	Modified residue	292	1	Phosphoserine Ref.6
	Modified residue	338	1	Phosphoserine Ref.7
	Modified residue	581	1	Phosphoserine Ref.6
	Modified residue	849	1	Phosphoserine Ref.7
	Modified residue	854	1	Phosphoserine Ref.7
	Modified residue	991	1	Phosphoserine Ref.7
	Modified residue	1045	1	Phosphotyrosine Ref.7
	Modified residue	1240	1	Phosphoserine Ref.7
	Modified residue	1242	1	Phosphoserine Ref.7
	Modified residue	1308	1	Phosphoserine Ref.7
	Modified residue	1315	1	Phosphoserine Ref.7
	Modified residue	1340	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 438	438	Missing in isoform A.		VSP_015117
Experimental info
	Sequence conflict	99	1	P → A in AAM29658. Ref.4
	Sequence conflict	206	1	P → S in AAF35862. Ref.1
	Sequence conflict	219	1	S → R in AAF35862. Ref.1
	Sequence conflict	242	1	A → E in AAM29658. Ref.4
	Sequence conflict	371	1	T → A in AAM29658. Ref.4
	Sequence conflict	398	1	S → T in AAM29658. Ref.4
	Sequence conflict	407	1	Y → H in AAM29658. Ref.4
	Sequence conflict	549	1	N → T in AAF35862. Ref.1
	Sequence conflict	549	1	N → T in AAM29658. Ref.4
	Sequence conflict	830	1	L → V in AAF35862. Ref.1
	Sequence conflict	1378	1	T → A in AAF35862. Ref.1
	Sequence conflict	1378	1	T → A in AAM49837. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform C [UniParc]. Last modified August 16, 2005. Version 4. Checksum: B90DC1BB792454EF	FASTA	1,403	150,937
	10 20 30 40 50 60 MSAKQSGSPK AGTSPLDKRV LQPKAQQQQQ QTKQTASQNN NAQNTASNKK TQQQQQPQQG 70 80 90 100 110 120 ATTTTQTTTA AAAAATAAAA ATAATTSAAA ATSSAAAVPA ATTETATTTT VDASQADALA 130 140 150 160 170 180 KVVVKDKKKV NQKASDFSNV GDWPTLIGGT SGSGKATSNE PKRNPTKKQQ SAKTAAAVAA 190 200 210 220 230 240 SSNTTSSEVA TESNVAGTTS SSNSNPSSST TTTNTTTNSQ ATTAPVASTS HDAKAQRDAE 250 260 270 280 290 300 PAANSAAGTT GTAAGPALTK KIPKHKWRPL PIDLAKSSRP KPIGGRPNRR FSDDIADQRR 310 320 330 340 350 360 PPRVYHDRQP GAGGAGVESR HPHAGRHPYG SRPATATSER VDSWRSSSST TTAAFDEQRS 370 380 390 400 410 420 GAAGGAGAAG TGVGSATRGP RRYRTPYRGG RQGRGGFSRQ GPGRPTYRIP RHLLASGEYA 430 440 450 460 470 480 NYLPADAAGA DSQSSYVLMG THYFGNVPAA YIELDANSIK EAIKKQVEYY FSVDNLTGDF 490 500 510 520 530 540 FLRRKMDPEG YIPVTLIASF HRVLALTTDV AVIVNAIKES DKLELFEGYK VRTKTTPTTW 550 560 570 580 590 600 PITEVPEVNE GEPKAIGTLE QEQLEQNDGQ EKLEEQTEAD SPPPILTSAM ATKPLNSIPP 610 620 630 640 650 660 PPMPRNPQNL VPKMLQDKQQ SRSSTIAALN SVNAISALTQ QVEGGAAELA GHLSGLAESV 670 680 690 700 710 720 KPKSTSTPDK RNAASAGNGA GSAAALVAEP EGIWKEVKRR SKTNAIKENA TTPPQQQQPP 730 740 750 760 770 780 LSQTLNNNND NVKTNNTSSK SKSSSNNAPS NASSSATVCV TTNNASSATK ATTKTTTTST 790 800 810 820 830 840 ATTTTNNNIK SGNAAYSKTH SKSSSKTAAP PSAQCHAEKE ELDFQFDEEL MDPLPPGTGR 850 860 870 880 890 900 INNFTENFSD DDESDYEFAD RDINKLLIVA QVGRAPKHEG YDRTADFTSR TKITQDLENI 910 920 930 940 950 960 INDGLVNYEE DLWTTTNVVA DYKTVNVISQ ADFEKLAGGR NKSVLPPQVV PPPPPFEEDL 970 980 990 1000 1010 1020 DETLVGDTTL NSTLNNTLKS RRARFYAAPN SHSIDPRTPR KRKLRHTANP PVEAHVGWLL 1030 1040 1050 1060 1070 1080 DTVEHRPRTT SMGSSAGTSP TASSYGSFGS SVPQSLPVFQ HPSHALLKEN NFTQQAYHKY 1090 1100 1110 1120 1130 1140 HSRCLKERRR LGYGQSQEMN TLYRFWSFFL RENFNKSMYN EFRSLALEDA GNGFRYGLEC 1150 1160 1170 1180 1190 1200 LFRFFSYGLE KKFRPNIYQD FQDETIADYE TGQLYGLEKF WAFLKYYKNG EKLEVQPKLA 1210 1220 1230 1240 1250 1260 EYLKSFKNIE DFRVVEPEIN EMLQGVGSLN PGRQLNRHRS VSESDGTAVI AAGGRRLNTT 1270 1280 1290 1300 1310 1320 ITNRSDYVGR LLQQQHQQQQ QQQHHQYQQG YGGYNQQQNR RRTGSFGSTT VRIRSGSLGN 1330 1340 1350 1360 1370 1380 KPQVANRNQG SQHELRRGGS NSGLAPHKRQ QQQKPKPGAG SQTGSTRATT SAAATATTAA 1390 1400 SAATSTAATP AVTVSSGSSS SKK
	Isoform A (B). Checksum: 83B79ACAF6B9FB66	FASTA	965	106,221
	10 20 30 40 50 60 MGTHYFGNVP AAYIELDANS IKEAIKKQVE YYFSVDNLTG DFFLRRKMDP EGYIPVTLIA 70 80 90 100 110 120 SFHRVLALTT DVAVIVNAIK ESDKLELFEG YKVRTKTTPT TWPITEVPEV NEGEPKAIGT 130 140 150 160 170 180 LEQEQLEQND GQEKLEEQTE ADSPPPILTS AMATKPLNSI PPPPMPRNPQ NLVPKMLQDK 190 200 210 220 230 240 QQSRSSTIAA LNSVNAISAL TQQVEGGAAE LAGHLSGLAE SVKPKSTSTP DKRNAASAGN 250 260 270 280 290 300 GAGSAAALVA EPEGIWKEVK RRSKTNAIKE NATTPPQQQQ PPLSQTLNNN NDNVKTNNTS 310 320 330 340 350 360 SKSKSSSNNA PSNASSSATV CVTTNNASSA TKATTKTTTT STATTTTNNN IKSGNAAYSK 370 380 390 400 410 420 THSKSSSKTA APPSAQCHAE KEELDFQFDE ELMDPLPPGT GRINNFTENF SDDDESDYEF 430 440 450 460 470 480 ADRDINKLLI VAQVGRAPKH EGYDRTADFT SRTKITQDLE NIINDGLVNY EEDLWTTTNV 490 500 510 520 530 540 VADYKTVNVI SQADFEKLAG GRNKSVLPPQ VVPPPPPFEE DLDETLVGDT TLNSTLNNTL 550 560 570 580 590 600 KSRRARFYAA PNSHSIDPRT PRKRKLRHTA NPPVEAHVGW LLDTVEHRPR TTSMGSSAGT 610 620 630 640 650 660 SPTASSYGSF GSSVPQSLPV FQHPSHALLK ENNFTQQAYH KYHSRCLKER RRLGYGQSQE 670 680 690 700 710 720 MNTLYRFWSF FLRENFNKSM YNEFRSLALE DAGNGFRYGL ECLFRFFSYG LEKKFRPNIY 730 740 750 760 770 780 QDFQDETIAD YETGQLYGLE KFWAFLKYYK NGEKLEVQPK LAEYLKSFKN IEDFRVVEPE 790 800 810 820 830 840 INEMLQGVGS LNPGRQLNRH RSVSESDGTA VIAAGGRRLN TTITNRSDYV GRLLQQQHQQ 850 860 870 880 890 900 QQQQQHHQYQ QGYGGYNQQQ NRRRTGSFGS TTVRIRSGSL GNKPQVANRN QGSQHELRRG 910 920 930 940 950 960 GSNSGLAPHK RQQQQKPKPG AGSQTGSTRA TTSAAATATT AASAATSTAA TPAVTVSSGS SSSKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"dlarp, a new candidate Hox target in Drosophila whose orthologue in mouse is expressed at sites of epithelium/mesenchymal interactions." Chauvet S., Maurel-Zaffran C., Miassod R., Jullien N., Pradel J., Aragnol D. Dev. Dyn. 218:401-413(2000) [PubMed: 10878606] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, INITIATION CODON ATA.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley. Tissue: Embryo and Ovary.
[5]	"Green fluorescent protein tagging Drosophila proteins at their native genomic loci with small P elements." Clyne P.J., Brotman J.S., Sweeney S.T., Davis G. Genetics 165:1433-1441(2003) [PubMed: 14668392] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed: 17372656] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-292 AND SER-581, MASS SPECTROMETRY.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-338; SER-849; SER-854; SER-991; TYR-1045; SER-1240; SER-1242; SER-1308; SER-1315 AND SER-1340, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF221108 mRNA. Translation: AAF35862.1. Sequence problems. AE014297 Genomic DNA. Translation: AAN14138.4. AE014297 Genomic DNA. Translation: AAN14139.1. AE014297 Genomic DNA. Translation: AAF56783.3. AY113653 mRNA. Translation: AAM29658.1. Sequence problems. AY118468 mRNA. Translation: AAM49837.1. Different initiation.
RefSeq	NP_524998.1. NP_733244.4. NP_733245.1.
UniGene	Dm.20475
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9VAW5. 1 interaction.
Genome annotation databases
Ensembl	FBgn0040108. Drosophila melanogaster. [Contig view]
GeneID	53567.
KEGG	dme:Dmel_CG14066.
NMPDR	fig|7227.3.peg.15082.
Organism-specific databases
FlyBase	FBgn0040108. larp.
Phylogenomic databases
HOGENOM	Q9VAW5.
Gene expression databases
ArrayExpress	Q9VAW5.
GermOnline	CG14066. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006607. DM15. IPR006630. Lupus_La_RNA_bd. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF05383. La. 1 hit. [Graphical view]
SMART	SM00684. DM15. 3 hits. SM00715. LA. 1 hit. [Graphical view]
PROSITE	PS50961. HTH_LA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	841422.

Entry information

Entry name

LARP_DROME

Accession

Primary (citable) accession number: Q9VAW5
Secondary accession number(s): A4V3J4 , Q8IMM4, Q8MSZ4, Q8MYR3, Q9NHN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	August 16, 2005
Last modified:	June 16, 2009
This is version 61 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project