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Q9VAM9 (MESH1_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1

EC=3.1.7.2
Alternative name(s):
Metazoan SpoT homolog 1
Short name=Mesh1
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name=(ppGpp)ase
Gene names
Name:Mesh1
ORF Names:CG11900
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length179 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ppGpp hydrolyzing enzyme involved in starvation response. Ref.4

Catalytic activity

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate. Ref.4

Cofactor

Manganese. Ref.4

Disruption phenotype

Induces retarded body growth and impaired starvation resistance. Mutants have highly down-regulated DNA and protein synthesis-related genes and up-regulated stress-responsible genes. Ref.4

Sequence similarities

Belongs to the MESH1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 179179Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
PRO_0000402127

Sites

Active site661Nucleophile Probable
Active site671Nucleophile Probable
Metal binding361Manganese
Metal binding621Manganese
Metal binding631Manganese
Metal binding1231Manganese

Secondary structure

........................ 179
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9VAM9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 9227D3DA9AD4E983

FASTA17920,569
        10         20         30         40         50         60 
MATYPSAKFM ECLQYAAFKH RQQRRKDPQE TPYVNHVINV STILSVEACI TDEGVLMAAL 

        70         80         90        100        110        120 
LHDVVEDTDA SFEDVEKLFG PDVCGLVREV TDDKSLEKQE RKRLQIENAA KSSCRAKLIK 

       130        140        150        160        170 
LADKLDNLRD LQVNTPTGWT QERRDQYFVW AKKVVDNLRG TNANLELKLD EIFRQRGLL 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[3]Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J., Park S., Wan K., Yu C., Celniker S.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"A metazoan ortholog of SpoT hydrolyzes ppGpp and functions in starvation responses."
Sun D., Lee G., Lee J.H., Kim H.-Y., Rhee H.W., Park S.-Y., Kim K.J., Kim Y., Kim B.Y., Hong J.-I., Park C., Choy H.E., Kim J.H., Jeon Y.H., Chung J.
Nat. Struct. Mol. Biol. 17:1188-1194(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS), DISRUPTION PHENOTYPE, FUNCTION, CATALYTIC ACTIVITY, ACTIVE SITE, COFACTOR, MANGANESE-BINDING SITES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014297 Genomic DNA. Translation: AAF56875.1.
BT024375 mRNA. Translation: ABC86437.1.
RefSeqNP_651682.1. NM_143425.2.
UniGeneDm.24112.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NQWX-ray2.90A/B1-179[»]
ProteinModelPortalQ9VAM9.
SMRQ9VAM9. Positions 2-179.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7227.FBpp0084776.

Proteomic databases

PaxDbQ9VAM9.
PRIDEQ9VAM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0085407; FBpp0084776; FBgn0039650.
GeneID43456.
KEGGdme:Dmel_CG11900.
UCSCCG11900-RA. d. melanogaster.

Organism-specific databases

CTD43456.
FlyBaseFBgn0039650. Mesh1.

Phylogenomic databases

eggNOGCOG0317.
GeneTreeENSGT00390000011608.
InParanoidQ9VAM9.
KOK01139.
OMANRCTPEG.
OrthoDBEOG7X3QTC.
PhylomeDBQ9VAM9.

Gene expression databases

BgeeQ9VAM9.

Family and domain databases

InterProIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamPF01966. HD. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi43456.
NextBio834034.
PROQ9VAM9.

Entry information

Entry nameMESH1_DROME
AccessionPrimary (citable) accession number: Q9VAM9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: March 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase