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Q9VAM9

- MESH1_DROME

UniProt

Q9VAM9 - MESH1_DROME

Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1

Gene

Mesh1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    ppGpp hydrolyzing enzyme involved in starvation response.1 Publication

    Catalytic activityi

    Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.1 Publication

    Cofactori

    Manganese.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361Manganese
    Metal bindingi62 – 621Manganese
    Metal bindingi63 – 631Manganese
    Active sitei66 – 661Nucleophile1 Publication
    Active sitei67 – 671Nucleophile1 Publication
    Metal bindingi123 – 1231Manganese

    GO - Molecular functioni

    1. guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity Source: FlyBase
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoric diester hydrolase activity Source: InterPro

    GO - Biological processi

    1. guanosine tetraphosphate catabolic process Source: FlyBase
    2. response to starvation Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 (EC:3.1.7.2)
    Alternative name(s):
    Metazoan SpoT homolog 1
    Short name:
    Mesh1
    Penta-phosphate guanosine-3'-pyrophosphohydrolase
    Short name:
    (ppGpp)ase
    Gene namesi
    Name:Mesh1
    ORF Names:CG11900
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0039650. Mesh1.

    Pathology & Biotechi

    Disruption phenotypei

    Induces retarded body growth and impaired starvation resistance. Mutants have highly down-regulated DNA and protein synthesis-related genes and up-regulated stress-responsible genes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 179179Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1PRO_0000402127Add
    BLAST

    Proteomic databases

    PaxDbiQ9VAM9.
    PRIDEiQ9VAM9.

    Expressioni

    Gene expression databases

    BgeeiQ9VAM9.

    Interactioni

    Protein-protein interaction databases

    STRINGi7227.FBpp0084776.

    Structurei

    Secondary structure

    1
    179
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1913
    Beta strandi26 – 294
    Helixi34 – 4512
    Turni46 – 483
    Helixi53 – 608
    Turni61 – 633
    Helixi64 – 674
    Helixi72 – 798
    Helixi81 – 899
    Helixi98 – 10710
    Helixi114 – 13320
    Helixi141 – 15818
    Helixi163 – 17614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3NQWX-ray2.90A/B1-179[»]
    ProteinModelPortaliQ9VAM9.
    SMRiQ9VAM9. Positions 2-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the MESH1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0317.
    GeneTreeiENSGT00390000011608.
    InParanoidiQ9VAM9.
    KOiK01139.
    OMAiYVNHVIN.
    OrthoDBiEOG7X3QTC.
    PhylomeDBiQ9VAM9.

    Family and domain databases

    InterProiIPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9VAM9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATYPSAKFM ECLQYAAFKH RQQRRKDPQE TPYVNHVINV STILSVEACI    50
    TDEGVLMAAL LHDVVEDTDA SFEDVEKLFG PDVCGLVREV TDDKSLEKQE 100
    RKRLQIENAA KSSCRAKLIK LADKLDNLRD LQVNTPTGWT QERRDQYFVW 150
    AKKVVDNLRG TNANLELKLD EIFRQRGLL 179
    Length:179
    Mass (Da):20,569
    Last modified:May 1, 2000 - v1
    Checksum:i9227D3DA9AD4E983
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF56875.1.
    BT024375 mRNA. Translation: ABC86437.1.
    RefSeqiNP_651682.1. NM_143425.2.
    UniGeneiDm.24112.

    Genome annotation databases

    EnsemblMetazoaiFBtr0085407; FBpp0084776; FBgn0039650.
    GeneIDi43456.
    KEGGidme:Dmel_CG11900.
    UCSCiCG11900-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAF56875.1 .
    BT024375 mRNA. Translation: ABC86437.1 .
    RefSeqi NP_651682.1. NM_143425.2.
    UniGenei Dm.24112.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3NQW X-ray 2.90 A/B 1-179 [» ]
    ProteinModelPortali Q9VAM9.
    SMRi Q9VAM9. Positions 2-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0084776.

    Proteomic databases

    PaxDbi Q9VAM9.
    PRIDEi Q9VAM9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0085407 ; FBpp0084776 ; FBgn0039650 .
    GeneIDi 43456.
    KEGGi dme:Dmel_CG11900.
    UCSCi CG11900-RA. d. melanogaster.

    Organism-specific databases

    CTDi 43456.
    FlyBasei FBgn0039650. Mesh1.

    Phylogenomic databases

    eggNOGi COG0317.
    GeneTreei ENSGT00390000011608.
    InParanoidi Q9VAM9.
    KOi K01139.
    OMAi YVNHVIN.
    OrthoDBi EOG7X3QTC.
    PhylomeDBi Q9VAM9.

    Miscellaneous databases

    GenomeRNAii 43456.
    NextBioi 834034.
    PROi Q9VAM9.

    Gene expression databases

    Bgeei Q9VAM9.

    Family and domain databases

    InterProi IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J., Park S., Wan K., Yu C., Celniker S.
      Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS), DISRUPTION PHENOTYPE, FUNCTION, CATALYTIC ACTIVITY, ACTIVE SITE, COFACTOR, MANGANESE-BINDING SITES.

    Entry informationi

    Entry nameiMESH1_DROME
    AccessioniPrimary (citable) accession number: Q9VAM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3