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Protein

Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1

Gene

Mesh1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ppGpp hydrolyzing enzyme involved in starvation response.1 Publication

Catalytic activityi

Guanosine 3',5'-bis(diphosphate) + H2O = guanosine 5'-diphosphate + diphosphate.1 Publication

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Manganese1
Metal bindingi62Manganese1
Metal bindingi63Manganese1
Active sitei66Nucleophile1 Publication1
Active sitei67Nucleophile1 Publication1
Metal bindingi123Manganese1

GO - Molecular functioni

GO - Biological processi

  • guanosine tetraphosphate catabolic process Source: FlyBase
  • response to starvation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1 (EC:3.1.7.2)
Alternative name(s):
Metazoan SpoT homolog 1
Short name:
Mesh1
Penta-phosphate guanosine-3'-pyrophosphohydrolase
Short name:
(ppGpp)ase
Gene namesi
Name:Mesh1
ORF Names:CG11900
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039650. Mesh1.

Pathology & Biotechi

Disruption phenotypei

Induces retarded body growth and impaired starvation resistance. Mutants have highly down-regulated DNA and protein synthesis-related genes and up-regulated stress-responsible genes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004021271 – 179Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1Add BLAST179

Proteomic databases

PaxDbiQ9VAM9.
PRIDEiQ9VAM9.

Expressioni

Gene expression databases

BgeeiFBgn0039650.
GenevisibleiQ9VAM9. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0084776.

Structurei

Secondary structure

1179
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 19Combined sources13
Beta strandi26 – 29Combined sources4
Helixi34 – 45Combined sources12
Turni46 – 48Combined sources3
Helixi53 – 60Combined sources8
Turni61 – 63Combined sources3
Helixi64 – 67Combined sources4
Helixi72 – 79Combined sources8
Helixi81 – 89Combined sources9
Helixi98 – 107Combined sources10
Helixi114 – 133Combined sources20
Helixi141 – 158Combined sources18
Helixi163 – 176Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NQWX-ray2.90A/B1-179[»]
ProteinModelPortaliQ9VAM9.
SMRiQ9VAM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MESH1 family.Curated

Phylogenomic databases

eggNOGiKOG1157. Eukaryota.
COG0317. LUCA.
GeneTreeiENSGT00390000011608.
InParanoidiQ9VAM9.
KOiK01139.
OMAiQRRKDPQ.
OrthoDBiEOG091G0L17.
PhylomeDBiQ9VAM9.

Family and domain databases

InterProiIPR003607. HD/PDEase_dom.
[Graphical view]
PfamiPF13328. HD_4. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9VAM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYPSAKFM ECLQYAAFKH RQQRRKDPQE TPYVNHVINV STILSVEACI
60 70 80 90 100
TDEGVLMAAL LHDVVEDTDA SFEDVEKLFG PDVCGLVREV TDDKSLEKQE
110 120 130 140 150
RKRLQIENAA KSSCRAKLIK LADKLDNLRD LQVNTPTGWT QERRDQYFVW
160 170
AKKVVDNLRG TNANLELKLD EIFRQRGLL
Length:179
Mass (Da):20,569
Last modified:May 1, 2000 - v1
Checksum:i9227D3DA9AD4E983
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56875.1.
BT024375 mRNA. Translation: ABC86437.1.
RefSeqiNP_651682.1. NM_143425.2.
UniGeneiDm.24112.

Genome annotation databases

EnsemblMetazoaiFBtr0085407; FBpp0084776; FBgn0039650.
GeneIDi43456.
KEGGidme:Dmel_CG11900.
UCSCiCG11900-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56875.1.
BT024375 mRNA. Translation: ABC86437.1.
RefSeqiNP_651682.1. NM_143425.2.
UniGeneiDm.24112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3NQWX-ray2.90A/B1-179[»]
ProteinModelPortaliQ9VAM9.
SMRiQ9VAM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0084776.

Proteomic databases

PaxDbiQ9VAM9.
PRIDEiQ9VAM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085407; FBpp0084776; FBgn0039650.
GeneIDi43456.
KEGGidme:Dmel_CG11900.
UCSCiCG11900-RA. d. melanogaster.

Organism-specific databases

CTDi43456.
FlyBaseiFBgn0039650. Mesh1.

Phylogenomic databases

eggNOGiKOG1157. Eukaryota.
COG0317. LUCA.
GeneTreeiENSGT00390000011608.
InParanoidiQ9VAM9.
KOiK01139.
OMAiQRRKDPQ.
OrthoDBiEOG091G0L17.
PhylomeDBiQ9VAM9.

Miscellaneous databases

GenomeRNAii43456.
PROiQ9VAM9.

Gene expression databases

BgeeiFBgn0039650.
GenevisibleiQ9VAM9. DM.

Family and domain databases

InterProiIPR003607. HD/PDEase_dom.
[Graphical view]
PfamiPF13328. HD_4. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMESH1_DROME
AccessioniPrimary (citable) accession number: Q9VAM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.