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Protein

Cadherin-99C

Gene

Cad99C

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cadherin that functions in epithelial morphogenesis and the intestine epithelial immune response (PubMed:16260500, PubMed:16507588, PubMed:24718992, PubMed:25639794). Essential for female fertility (PubMed:16260500, PubMed:16507588). Regulates the length and organization of apical microvilli in developing follicle cells and salivary glands (PubMed:16260500, PubMed:16507588, PubMed:24718992, PubMed:25236597). Function in the follicle cell is essential for egg development as the microvilli secrete eggshell material such as the vitelline membrane (PubMed:16260500, PubMed:16507588, PubMed:25236597). Acts at least in part by regulating the recruitment of the myosin ck to the follicle cell microvilli (PubMed:25236597). Also required to regulate cell rearrangements during salivary tube elongation, possibly by modulating cellular adhesion between the apical surface and apical extracellular matrix during epithelial tube elongation (PubMed:24718992). May also function in cellular adhesion during the development of other tubular epithelia such as the trachea (PubMed:24718992). Possibly functions as an apical membrane determinant which acts in apical membrane expansion during salivary and tracheal epithelial tube elongation (PubMed:24718992). In salivary gland development, this function is independent of the other apical membrane determinants crb and sas (PubMed:24718992). Essential downstream component of a hh-signaling pathway which regulates the Duox-dependent gut epithelial immune response to bacterial uracil; required for endosome formation in the enterocyte and activating norpA-dependent Ca2+ mobilization, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794).5 Publications

GO - Molecular functioni

  • calcium ion binding Source: FlyBase
  • myosin binding Source: FlyBase

GO - Biological processi

  • apical protein localization Source: FlyBase
  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: FlyBase
  • calcium-mediated signaling Source: FlyBase
  • cell-cell adhesion mediated by cadherin Source: FlyBase
  • chorion-containing eggshell formation Source: FlyBase
  • follicle cell microvillus organization Source: FlyBase
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
  • mucosal immune response Source: FlyBase
  • positive regulation of clathrin-dependent endocytosis Source: FlyBase
  • regulation of follicle cell microvillus length Source: FlyBase
  • smoothened signaling pathway Source: FlyBase
  • tube morphogenesis Source: FlyBase
  • vitelline membrane formation involved in chorion-containing eggshell formation Source: FlyBase

Keywordsi

Biological processImmunity
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
Cadherin-99C1 Publication
Gene namesi
Name:Cad99C1 PublicationImported
ORF Names:CG31009Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0039709. Cad99C.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 1395ExtracellularCuratedAdd BLAST1367
Transmembranei1396 – 1416HelicalSequence analysisAdd BLAST21
Topological domaini1417 – 1706CytoplasmicCuratedAdd BLAST290

GO - Cellular componenti

  • apical plasma membrane Source: FlyBase
  • endosome membrane Source: UniProtKB-SubCell
  • integral component of plasma membrane Source: FlyBase
  • microvillus Source: FlyBase
  • microvillus membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell membrane, Cell projection, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Females display reduced fertility as well as defective eggshell development (PubMed:16260500). Ovarian follicle cell microvilli are abnormally shaped and display a range of defects including decreased length, reduced number and an irregular distribution pattern, leading to impaired vitelline membrane formation (PubMed:16260500, PubMed:16507588). In the intestines of 3-day old adults there is no formation of endosomes and consequently no DUOX-dependent up-regulation of reactive oxygen species (ROS) in response to the ingestion of bacteria-derived uracil (PubMed:25639794). Embryos display irregular cell rearrangements during salivary gland development with fewer cells surrounding the salivary gland lumens and lumens appear to be elongated (PubMed:24718992).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_500818059829 – 1706Cadherin-99CSequence analysisAdd BLAST1678

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi105N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi188N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi442N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi553N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi620N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi753N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi1053N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi1088N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi1108N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi1311N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi1367N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9VAF5.
PRIDEiQ5U133.

Expressioni

Developmental stagei

Low expression throughout the imaginal disks of third instar larvae with higher levels of expression at the anterior-posterior boundary (at protein level) (PubMed:15708564). Detected in the germarium and stage 2 and stage 3 egg chambers (at protein level) (PubMed:16507588). From stages 2 to 8, expressed in the follicle cells at the anterior and posterior poles of the egg chambers (at protein level) (PubMed:16260500, PubMed:16507588). From stage 4 to the end of oogenesis, only detected in follicle cells that are in contact with the oocyte (at protein level) (PubMed:16260500, PubMed:16507588). From stage 11 expressed at the apical surface of several embryonic tubular organs including the salivary glands, trachea, foregut and hindgut (PubMed:24718992).4 Publications

Inductioni

Up-regulated in the adult anterior midgut after the ingestion of bacterial uracil. Strong up-regulation detected 1 hour, 2 hours and 16 hours after ingestion, whereas no up-regulation was detected at 4 hours.1 Publication

Gene expression databases

BgeeiFBgn0039709.
ExpressionAtlasiQ9VAF5. differential.

Interactioni

Subunit structurei

Interacts (via the cytoplasmic domain) with ck.1 Publication

GO - Molecular functioni

  • myosin binding Source: FlyBase

Protein-protein interaction databases

IntActiQ9VAF5. 2 interactors.
MINTiMINT-321696.
STRINGi7227.FBpp0292214.

Structurei

3D structure databases

ProteinModelPortaliQ9VAF5.
SMRiQ9VAF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 142Cadherin 1PROSITE-ProRule annotationAdd BLAST75
Domaini143 – 264Cadherin 2PROSITE-ProRule annotationAdd BLAST122
Domaini277 – 387Cadherin 3PROSITE-ProRule annotationAdd BLAST111
Domaini388 – 500Cadherin 4PROSITE-ProRule annotationAdd BLAST113
Domaini519 – 604Cadherin 5PROSITE-ProRule annotationAdd BLAST86
Domaini605 – 704Cadherin 6PROSITE-ProRule annotationAdd BLAST100
Domaini707 – 807Cadherin 7PROSITE-ProRule annotationAdd BLAST101
Domaini808 – 908Cadherin 8PROSITE-ProRule annotationAdd BLAST101
Domaini909 – 1005Cadherin 9PROSITE-ProRule annotationAdd BLAST97
Domaini1038 – 1148Cadherin 10PROSITE-ProRule annotationAdd BLAST111
Domaini1156 – 1270Cadherin 11PROSITE-ProRule annotationAdd BLAST115

Domaini

The extracellular domain is necessary for conferring apical characteristics on the salivary gland membrane (PubMed:24718992). Necessary for regulating salivary gland membrane expansion and tube length, but is not required for cell rearrangement during tube elongation (PubMed:24718992). Sufficient to promote the outgrowth of follicle cell microvilli and the formation of microvilli bundles (PubMed:16260500, PubMed:24718992) Sufficient to promote formation of microvilli-like structures in the salivary glands and the follicle cell microvilli bundles (PubMed:16507588, PubMed:24718992). Dispensable for apical localization (PubMed:24718992).3 Publications
The cytoplasmic domain is necessary for the formation of uracil-induced endosomes.1 Publication
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain (By similarity).By similarity

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3594. Eukaryota.
ENOG410XQHI. LUCA.
GeneTreeiENSGT00880000138007.
KOiK16500.
OMAiGPNTKPP.
OrthoDBiEOG091G0MMW.

Family and domain databases

InterProiView protein in InterPro
IPR002126. Cadherin.
IPR015919. Cadherin-like.
IPR020894. Cadherin_CS.
PfamiView protein in Pfam
PF00028. Cadherin. 8 hits.
PRINTSiPR00205. CADHERIN.
SMARTiView protein in SMART
SM00112. CA. 11 hits.
SUPFAMiSSF49313. SSF49313. 10 hits.
PROSITEiView protein in PROSITE
PS00232. CADHERIN_1. 3 hits.
PS50268. CADHERIN_2. 11 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9VAF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAARNSLTPQ QGLGFFGLLI LLCSAVLGKS QMCEVETGQT NIILDIEESR
60 70 80 90 100
ESFIGQPTTP PELPIFGDPD TEIALNLVFP KGQPIFQLNG KKLQLLQPLD
110 120 130 140 150
RDEENLSHIV FQVSCTTRST GKKRTIPIIV RVSDINDNAP RFMNTPYEVT
160 170 180 190 200
VPESTPVGTT IFRNIQALDK DAGVNGLVEY FIAEGSPNST NVEKYSADGY
210 220 230 240 250
GTFAISFPHQ GQVTVAKTLD FEKIQTYYLT IVASDRARNT ADRLSSTTTL
260 270 280 290 300
TVNIADSNDL DPSFIYSGCV SLDGACINPE YSASVPAGSL LGVLTVLPER
310 320 330 340 350
IQAVDLDTIN SPIRYSFASG MPGNYADYFQ IDESTGVLKQ TKAVDTSTAK
360 370 380 390 400
KYDIIVKAEE VSPGPQRFTT AKLEIFVKPV DANPPVISSS QAEGYVDENS
410 420 430 440 450
PIGTRVLDAH GNPISFMTTD ADLSDSDPKP DYIYELTTPS FNVTGDGILV
460 470 480 490 500
VNEENLDRDP PAPGRFKFQV VAREPRTNAA SAPLSLTVHL RDVNDNAPKL
510 520 530 540 550
AMVAPISITA GDQSESRLVT QVTATDNDEG PNAVVTYSIY HVSNNGIQKF
560 570 580 590 600
TINATTGEIR TQGRLLAGEQ YSITVQATDI GGLSSQAIVE VSVTPGPNTK
610 620 630 640 650
PPRFQKPIYE VQVSEGAEIN STVTVVHAED PENDAVVYSI ISGNDLRQFA
660 670 680 690 700
VGQESGVIIV IRKLDRESLT RYQLILKAED TGGLSSSATV NIKVTDINDK
710 720 730 740 750
NPEFEASTLP YVFQVEEGKA QASVGVVHAT DADEGINAEI TYSIPTDIPF
760 770 780 790 800
TINATSGEIL TAKELDYEQL NEYKFVVTAK DGAPDARLGT ASVTVMVLDL
810 820 830 840 850
PDEVPKFSDA RIDVHIPENE ENFLVATVQA FDPDSMPEIT YVLRKGNAEL
860 870 880 890 900
FKVSEKSGEV RTIKGLDYES QKQHQLTIGT IENDGNGPGD TILLVVDVED
910 920 930 940 950
RNDLPPRFIT VPDPVTVNDD QGIGTIIATL PAIDGDGTSP GNVVRYEIVG
960 970 980 990 1000
RGKAPKYFQV DPDTGAVRIR DELRKEEDTE YQVDIRAYDL GEPQLSSVAP
1010 1020 1030 1040 1050
LRVDVHHLLS SGNNEIKLDN KLESGTGMSS ESIGLAFSDD SYTTSVPESM
1060 1070 1080 1090 1100
EANSTLKLIQ IVNSKTSGDG PPAFRCEFVS GNGGGIFNLS SADHGCNLLL
1110 1120 1130 1140 1150
IQPLDFENKS SYSLQLRLTS HRYFVNPLKD TTSVEIIVQD ENDNAPEFEF
1160 1170 1180 1190 1200
NRLRGQQDTF YTVVTEEMDV DTTILQVRAT DRDSGKFGTV RYTLYDDDEN
1210 1220 1230 1240 1250
RVNMPTSFFM MSEDTGVLRT AKHFKNENDF PLTFLVEARD SDGQEQGSHR
1260 1270 1280 1290 1300
TRARIVVNKL ADINRMALSF PNAAPSDLRN YYTELEELLD KKTGLVSGIE
1310 1320 1330 1340 1350
RMSSQKYLAK NGSVIENPAA TDIWFYLIDP KTEQLVSRKD SIVETTLLEP
1360 1370 1380 1390 1400
AARSELNIAL PRATAENISF PLERKEHVHK VKAAVAIDNE VFPFTLIAIS
1410 1420 1430 1440 1450
LVILILGTIG IIYICISWSK YKNFKQRMRQ YSSTNPPRYD PVIVNQQASS
1460 1470 1480 1490 1500
ASETIANMKE YETQMLAMAV PPDVDDDLQL DFSAKNHAFS LDNVSYITHK
1510 1520 1530 1540 1550
ENTNGGGQSS PSHSDATTAT IATLRRHKNL NNASMNNNLA INNRQNTFNR
1560 1570 1580 1590 1600
TLEMNTRNNA NPLASPPNGA LSGTLTLGRI KHQNSNHYQN GAYNIDPTGP
1610 1620 1630 1640 1650
NNMAHAKNNA YSTMGRRGNT FGDVGLLNGN GELMNATLGR NGQLNNRLYG
1660 1670 1680 1690 1700
GEVPITNPLF QRSNSDHNHL SSTNENVSFG KRDYGQIGFS YLNDLDRSEV

ETTTEL
Length:1,706
Mass (Da):186,874
Last modified:June 1, 2003 - v2
Checksum:i2A2CB6F3D5561D8B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61P → A in AAX12106 (PubMed:15708564).Curated1
Sequence conflicti61P → A in AAX12107 (PubMed:15708564).Curated1
Sequence conflicti853V → I in AAX12106 (PubMed:15708564).Curated1
Sequence conflicti853V → I in AAX12107 (PubMed:15708564).Curated1
Sequence conflicti1083G → E in AAX12106 (PubMed:15708564).Curated1
Sequence conflicti1083G → E in AAX12107 (PubMed:15708564).Curated1
Sequence conflicti1331K → R in AAX12106 (PubMed:15708564).Curated1
Sequence conflicti1331K → R in AAX12107 (PubMed:15708564).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY853687 mRNA. Translation: AAX12107.1.
AY853686 mRNA. Translation: AAX12106.1.
AE014297 Genomic DNA. Translation: AAF56955.2.
AE014297 Genomic DNA. Translation: ADV37401.1.
BT016059 mRNA. Translation: AAV36944.1.
RefSeqiNP_001189311.1. NM_001202382.1.
NP_733314.1. NM_170435.2.
UniGeneiDm.4517.

Genome annotation databases

EnsemblMetazoaiFBtr0085488; FBpp0084854; FBgn0039709.
FBtr0303095; FBpp0292214; FBgn0039709.
GeneIDi43528.
KEGGidme:Dmel_CG31009.
UCSCiCG31009-RA. d. melanogaster.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCAD99_DROME
AccessioniPrimary (citable) accession number: Q9VAF5
Secondary accession number(s): Q5D716
, Q5U133, Q961C6, Q9VAF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2017
Last sequence update: June 1, 2003
Last modified: July 5, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase