Reviewed, UniProtKB/Swiss-Prot Q9VA73 (CMC_DROME)

Names and origin

Protein names

Recommended name:
    Calcium-binding mitochondrial carrier protein Aralar1

Gene names

Name:	aralar1
ORF Names:	CG2139

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Calcium-dependent mitochondrial solute carrier By similarity.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the mitochondrial carrier family. Contains 4 EF-hand domains. Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Transmembrane
Ligand	Calcium
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG13343	Q9V6U8	1	EBI-196947,EBI-121330

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9VA73-1)

Also known as: C;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: Q9VA73-2)

Also known as: A; D;

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MPMHIPFPFNWIPTLPVARCQE → MPLTKSLPN

Isoform 3 (identifier: Q9VA73-3)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MPMHIPFPFNWIPTLPVARCQ → MHDKD

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Calcium-binding mitochondrial carrier protein Aralar1		PRO_0000090606
Regions
	Transmembrane	346 – 363	18	1 Potential
	Transmembrane	406 – 425	20	2 Potential
	Transmembrane	449 – 462	14	3 Potential
	Transmembrane	498 – 517	20	4 Potential
	Transmembrane	537 – 554	18	5 Potential
	Transmembrane	594 – 613	20	6 Potential
	Domain	71 – 104	34	EF-hand 1
	Domain	105 – 140	36	EF-hand 2
	Domain	142 – 175	34	EF-hand 3
	Domain	176 – 211	36	EF-hand 4
	Repeat	340 – 431	92	Solcar 1
	Repeat	439 – 523	85	Solcar 2
	Repeat	531 – 619	89	Solcar 3
	Calcium binding	84 – 95	12	1
	Calcium binding	118 – 129	12	2
	Calcium binding	189 – 200	12	3
Natural variations
	Alternative sequence	1 – 22	22	MPMHI…ARCQE → MPLTKSLPN in isoform 2.		VSP_003266
	Alternative sequence	1 – 21	21	MPMHI…VARCQ → MHDKD in isoform 3.		VSP_003265
Experimental info
	Sequence conflict	356 – 357	2	GA → AP in CAB62169. Ref.1
	Sequence conflict	556	1	V → A in CAB62169. Ref.1
	Sequence conflict	653	1	V → L in CAB62169. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (C) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8EC93D92031F5B77	FASTA	695	76,754
	10 20 30 40 50 60 MPMHIPFPFN WIPTLPVARC QESPSLLKRA GTEKLREVFL KYASIQKNGE HYMTSEDFVR 70 80 90 100 110 120 KFLGLFSESA FNDESVRLLA NIADTSKDGL ISFSEFQAFE GLLCTPDALY RTAFQLFDRK 130 140 150 160 170 180 GNGTVSYADF ADVVQKTELH SKIPFSLDGP FIKRYFGDKK QRLINYAEFT QLLHDFHEEH 190 200 210 220 230 240 AMEAFRSKDP AGTGFISPLD FQDIIVNVKR HLLTPGVRDN LVSVTEGHKV SFPYFIAFTS 250 260 270 280 290 300 LLNNMELIKQ VYLHATEGSR TDMITKDQIL LAAQTMSQIT PLEIDILFHL AGAVHQAGRI 310 320 330 340 350 360 DYSDLSNIAP EHYTKHMTHR LAEIKAVESP ADRSAFIQVL ESSYRFTLGS FAGAVGATVV 370 380 390 400 410 420 YPIDLVKTRM QNQRAGSYIG EVAYRNSWDC FKKVVRHEGF MGLYRGLLPQ LMGVAPEKAI 430 440 450 460 470 480 KLTVNDLVRD KLTDKKGNIP TWAEVLAGGC AGASQVVFTN PLEIVKIRLQ VAGEIASGSK 490 500 510 520 530 540 IRAWSVVREL GLFGLYKGAR ACLLRDVPFS AIYFPTYAHT KAMMADKDGY NHPLTLLAAG 550 560 570 580 590 600 AIAGVPAASL VTPADVIKTR LQVVARSGQT TYTGVWDATK KIMAEEGPRA FWKGTAARVF 610 620 630 640 650 660 RSSPQFGVTL VTYELLQRLF YVDFGGTQPK GSEAHKITTP LEQAAASVTT ENVDHIGGYR 670 680 690 AAVPLLAGVE SKFGLYLPRF GRGVTAASPS TATGS
	Isoform 2 (A) (D). Checksum: DF4D6851D4715FC8	FASTA	682	75,129
	10 20 30 40 50 60 MPLTKSLPNS PSLLKRAGTE KLREVFLKYA SIQKNGEHYM TSEDFVRKFL GLFSESAFND 70 80 90 100 110 120 ESVRLLANIA DTSKDGLISF SEFQAFEGLL CTPDALYRTA FQLFDRKGNG TVSYADFADV 130 140 150 160 170 180 VQKTELHSKI PFSLDGPFIK RYFGDKKQRL INYAEFTQLL HDFHEEHAME AFRSKDPAGT 190 200 210 220 230 240 GFISPLDFQD IIVNVKRHLL TPGVRDNLVS VTEGHKVSFP YFIAFTSLLN NMELIKQVYL 250 260 270 280 290 300 HATEGSRTDM ITKDQILLAA QTMSQITPLE IDILFHLAGA VHQAGRIDYS DLSNIAPEHY 310 320 330 340 350 360 TKHMTHRLAE IKAVESPADR SAFIQVLESS YRFTLGSFAG AVGATVVYPI DLVKTRMQNQ 370 380 390 400 410 420 RAGSYIGEVA YRNSWDCFKK VVRHEGFMGL YRGLLPQLMG VAPEKAIKLT VNDLVRDKLT 430 440 450 460 470 480 DKKGNIPTWA EVLAGGCAGA SQVVFTNPLE IVKIRLQVAG EIASGSKIRA WSVVRELGLF 490 500 510 520 530 540 GLYKGARACL LRDVPFSAIY FPTYAHTKAM MADKDGYNHP LTLLAAGAIA GVPAASLVTP 550 560 570 580 590 600 ADVIKTRLQV VARSGQTTYT GVWDATKKIM AEEGPRAFWK GTAARVFRSS PQFGVTLVTY 610 620 630 640 650 660 ELLQRLFYVD FGGTQPKGSE AHKITTPLEQ AAASVTTENV DHIGGYRAAV PLLAGVESKF 670 680 GLYLPRFGRG VTAASPSTAT GS
	Isoform 3 (B). Checksum: 5F318AC5AFD7B1AA	FASTA	679	74,903
	10 20 30 40 50 60 MHDKDESPSL LKRAGTEKLR EVFLKYASIQ KNGEHYMTSE DFVRKFLGLF SESAFNDESV 70 80 90 100 110 120 RLLANIADTS KDGLISFSEF QAFEGLLCTP DALYRTAFQL FDRKGNGTVS YADFADVVQK 130 140 150 160 170 180 TELHSKIPFS LDGPFIKRYF GDKKQRLINY AEFTQLLHDF HEEHAMEAFR SKDPAGTGFI 190 200 210 220 230 240 SPLDFQDIIV NVKRHLLTPG VRDNLVSVTE GHKVSFPYFI AFTSLLNNME LIKQVYLHAT 250 260 270 280 290 300 EGSRTDMITK DQILLAAQTM SQITPLEIDI LFHLAGAVHQ AGRIDYSDLS NIAPEHYTKH 310 320 330 340 350 360 MTHRLAEIKA VESPADRSAF IQVLESSYRF TLGSFAGAVG ATVVYPIDLV KTRMQNQRAG 370 380 390 400 410 420 SYIGEVAYRN SWDCFKKVVR HEGFMGLYRG LLPQLMGVAP EKAIKLTVND LVRDKLTDKK 430 440 450 460 470 480 GNIPTWAEVL AGGCAGASQV VFTNPLEIVK IRLQVAGEIA SGSKIRAWSV VRELGLFGLY 490 500 510 520 530 540 KGARACLLRD VPFSAIYFPT YAHTKAMMAD KDGYNHPLTL LAAGAIAGVP AASLVTPADV 550 560 570 580 590 600 IKTRLQVVAR SGQTTYTGVW DATKKIMAEE GPRAFWKGTA ARVFRSSPQF GVTLVTYELL 610 620 630 640 650 660 QRLFYVDFGG TQPKGSEAHK ITTPLEQAAA SVTTENVDHI GGYRAAVPLL AGVESKFGLY 670 LPRFGRGVTA ASPSTATGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a second member of the subfamily of calcium-binding mitochondrial carriers expressed in human non-excitable tissues." Del Arco A., Agudo M., Satrustegui J. Biochem. J. 345:725-732(2000) [PubMed: 10642534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Ovary.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18197 mRNA. Translation: CAB62169.1. AE014297 Genomic DNA. Translation: AAF57048.1. AE014297 Genomic DNA. Translation: AAF57049.1. AE014297 Genomic DNA. Translation: AAF57050.3. AE014297 Genomic DNA. Translation: AAN14230.1. AY058654 mRNA. Translation: AAL13883.1.
RefSeq	NP_651795.2. NP_733364.1. NP_733365.1. NP_733366.2.
UniGene	Dm.20778
3D structure databases
SMR	Q9VA73. Positions 345-616.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17130N.
IntAct	Q9VA73. 2 interactions.
STRING	Q9VA73.
Proteomic databases
PRIDE	Q9VA73.
Genome annotation databases
Ensembl	FBtr0085692; FBpp0085054; FBgn0028646; Drosophila melanogaster. [Genome view] FBtr0085693; FBpp0085055; FBgn0028646; Drosophila melanogaster. [Genome view] FBtr0085694; FBpp0085056; FBgn0028646; Drosophila melanogaster. [Genome view] FBtr0085695; FBpp0085057; FBgn0028646; Drosophila melanogaster. [Genome view]
GeneID	43616.
KEGG	dme:Dmel_CG2139.
UCSC	CG2139-RA. d. melanogaster.
Organism-specific databases
CTD	43616.
FlyBase	FBgn0028646. aralar1.
Phylogenomic databases
InParanoid	Q9VA73.
OMA	MELIKRV.
OrthoDB	EOG9C880J.
PhylomeDB	Q9VA73.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-014170-MONOMER.
Gene expression databases
ArrayExpress	Q9VA73.
GermOnline	CG2139. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR002067. Mit_carrier. IPR001993. Mitochondria_substrate_carrier. IPR018108. Mitochondrial_sb/sol_carrier. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PANTHER	PTHR11896. Mitoch_carrier. 1 hit.
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00926. MITOCARRIER.
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 3 hits. PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	834864.

Entry information

Entry name

CMC_DROME

Accession

Primary (citable) accession number: Q9VA73
Secondary accession number(s): A4V3N6 , Q95TN5, Q9U5V8, Q9VA72, Q9VA74

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project