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Q9V9J3

- SRC42_DROME

UniProt

Q9V9J3 - SRC42_DROME

Protein

Tyrosine-protein kinase Src42A

Gene

Src42A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Essential for correct eye morphogenesis (ommatidial R7 neuron formation), this requires the Ras1/MAPK signal transduction pathway. May be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei276 – 2761ATPPROSITE-ProRule annotation
    Active sitei370 – 3701Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi254 – 2629ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein tyrosine kinase activity Source: UniProtKB

    GO - Biological processi

    1. actin filament bundle assembly Source: FlyBase
    2. adherens junction organization Source: FlyBase
    3. apoptotic cell clearance Source: FlyBase
    4. cell migration Source: FlyBase
    5. compound eye development Source: FlyBase
    6. dorsal closure Source: FlyBase
    7. dorsal closure, spreading of leading edge cells Source: FlyBase
    8. epithelial cell-cell adhesion Source: FlyBase
    9. imaginal disc-derived wing morphogenesis Source: FlyBase
    10. imaginal disc fusion, thorax closure Source: FlyBase
    11. JNK cascade Source: FlyBase
    12. negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
    13. negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
    14. open tracheal system development Source: FlyBase
    15. peptidyl-tyrosine phosphorylation Source: FlyBase
    16. protein phosphorylation Source: FlyBase
    17. salivary gland morphogenesis Source: FlyBase
    18. transmembrane receptor protein tyrosine kinase signaling pathway Source: FlyBase
    19. tricarboxylic acid cycle Source: FlyBase

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 1994.
    SignaLinkiQ9V9J3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase Src42A (EC:2.7.10.2)
    Alternative name(s):
    Tyrosine-protein kinase Src41
    Short name:
    Dsrc41
    Gene namesi
    Name:Src42A
    Synonyms:Src41, TK5
    ORF Names:CG44128
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0264959. Src42A.

    Subcellular locationi

    GO - Cellular componenti

    1. adherens junction Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 517517Tyrosine-protein kinase Src42APRO_0000088139Add
    BLAST

    Proteomic databases

    PaxDbiQ9V9J3.
    PRIDEiQ9V9J3.

    Expressioni

    Tissue specificityi

    Ubiquitous in early embryos, in stages 13-16 expression is seen in visceral mesoderm, hindgut, brain, anal pads and ventral ganglions. In larvae, expression is in CNS, wing disk, leg disk and photoreceptor precursors in the eye-antenna disks posterior to the morphogenetic furrow.1 Publication

    Developmental stagei

    In early embryos expression is very low, expression increases during embryogenesis. Also expressed in larvae and pupae.1 Publication

    Gene expression databases

    BgeeiQ9V9J3.

    Interactioni

    Protein-protein interaction databases

    BioGridi61430. 27 interactions.
    DIPiDIP-22220N.
    IntActiQ9V9J3. 2 interactions.
    MINTiMINT-333459.
    STRINGi7227.FBpp0085321.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9V9J3.
    SMRiQ9V9J3. Positions 37-515.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 12462SH3CuratedPROSITE-ProRule annotationAdd
    BLAST
    Domaini130 – 22293SH2CuratedPROSITE-ProRule annotationAdd
    BLAST
    Domaini248 – 504257Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.CuratedPROSITE-ProRule annotation
    Contains 1 SH3 domain.CuratedPROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00640000091347.
    InParanoidiQ9V9J3.
    KOiK08892.
    OMAiVSHYTKT.
    PhylomeDBiQ9V9J3.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9V9J3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNCLTTQKG EPDKPADRIK LDDPPTIGVG VGVPQIPMPS HAGQPPEQIR    50
    PVPQIPESET AGANAKIFVA LYDYDARTDE DLSFRKGEHL EILNDTQGDW 100
    WLARSKKTRS EGYIPSNYVA KLKSIEAEPW YFRKIKRIEA EKKLLLPENE 150
    HGAFLIRDSE SRHNDYSLSV RDGDTVKHYR IRQLDEGGFF IARRTTFRTL 200
    QELVEHYSKD SDGLCVNLCK PCVQIEKPVT EGLSHRTRDQ WEIDRTSLKF 250
    VRKLGSGQFG DVWEGLWNNT TPVAIKTLKS GTMDPKDFLA EAQIMKKLRH 300
    TKLIQLYAVC TVEEPIYIIT ELMKHGSLLE YLQAIAGKGR SLKMQTLIDM 350
    AAQIAAGMAY LESQNYIHRD LAARNVLVGD GNIVKIADFG LARLIKEDEY 400
    EARVGARFPI KWTAPEAANY SKFSIKSDVW SFGILLTELV TYGRIPYPGM 450
    TNAEVLTQVE HGYRMPQPPN CEPRLYEIML ECWHKDPMRR PTFETLQWKL 500
    EDFYTSDQSD YKEAQAY 517
    Length:517
    Mass (Da):59,069
    Last modified:May 1, 2000 - v1
    Checksum:i1EF196E4D7AE61E9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti65 – 651A → V in BAA07705. (PubMed:8682295)Curated
    Sequence conflicti376 – 3761V → I in AAB19907. (PubMed:1915852)Curated
    Sequence conflicti376 – 3761V → I in CAA05746. (PubMed:9731193)Curated
    Sequence conflicti381 – 3866GNIVKI → SNVVKM in AAB19907. (PubMed:1915852)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42125 mRNA. Translation: BAA07705.1.
    AE013599 Genomic DNA. Translation: AAF57295.1.
    AY058652 mRNA. Translation: AAL13881.1.
    S55977 Genomic DNA. Translation: AAB19907.1.
    AJ002911 Genomic DNA. Translation: CAA05746.1.
    RefSeqiNP_476849.1. NM_057501.4.
    UniGeneiDm.4134.

    Genome annotation databases

    EnsemblMetazoaiFBtr0085967; FBpp0085320; FBgn0004603.
    FBtr0085968; FBpp0085321; FBgn0004603.
    GeneIDi35524.
    KEGGidme:Dmel_CG44128.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D42125 mRNA. Translation: BAA07705.1 .
    AE013599 Genomic DNA. Translation: AAF57295.1 .
    AY058652 mRNA. Translation: AAL13881.1 .
    S55977 Genomic DNA. Translation: AAB19907.1 .
    AJ002911 Genomic DNA. Translation: CAA05746.1 .
    RefSeqi NP_476849.1. NM_057501.4.
    UniGenei Dm.4134.

    3D structure databases

    ProteinModelPortali Q9V9J3.
    SMRi Q9V9J3. Positions 37-515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 61430. 27 interactions.
    DIPi DIP-22220N.
    IntActi Q9V9J3. 2 interactions.
    MINTi MINT-333459.
    STRINGi 7227.FBpp0085321.

    Proteomic databases

    PaxDbi Q9V9J3.
    PRIDEi Q9V9J3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0085967 ; FBpp0085320 ; FBgn0004603 .
    FBtr0085968 ; FBpp0085321 ; FBgn0004603 .
    GeneIDi 35524.
    KEGGi dme:Dmel_CG44128.

    Organism-specific databases

    CTDi 35524.
    FlyBasei FBgn0264959. Src42A.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00640000091347.
    InParanoidi Q9V9J3.
    KOi K08892.
    OMAi VSHYTKT.
    PhylomeDBi Q9V9J3.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 1994.
    SignaLinki Q9V9J3.

    Miscellaneous databases

    ChiTaRSi Src42A. drosophila.
    GenomeRNAii 35524.
    NextBioi 793845.

    Gene expression databases

    Bgeei Q9V9J3.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00452. SH3DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src."
      Takahashi F., Endo S., Kojima T., Saigo K.
      Genes Dev. 10:1645-1656(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: Canton-S.
      Tissue: Pupae.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    5. "Identification of seven novel protein-tyrosine kinase genes of Drosophila by the polymerase chain reaction."
      Shishido E., Emori Y., Saigo K.
      FEBS Lett. 289:235-238(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-428.
    6. "Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA."
      Oates A.C., Wollberg P., Achen M.G., Wilks A.F.
      Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 376-427.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSRC42_DROME
    AccessioniPrimary (citable) accession number: Q9V9J3
    Secondary accession number(s): O18369
    , Q0E9P0, Q26297, Q94879
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2003
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3