Q9V9J3 (SRC42_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase Src42A
EC=2.7.10.2

Alternative name(s):
Tyrosine-protein kinase Src41
Short name=Dsrc41

Gene names

Name:	Src42A
Synonyms:	Src41, TK5
ORF Names:	CG7873

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for correct eye morphogenesis (ommatidial R7 neuron formation), this requires the Ras1/MAPK signal transduction pathway. May be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia. Ref.1
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Tissue specificity	Ubiquitous in early embryos, in stages 13-16 expression is seen in visceral mesoderm, hindgut, brain, anal pads and ventral ganglions. In larvae, expression is in CNS, wing disk, leg disk and photoreceptor precursors in the eye-antenna disks posterior to the morphogenetic furrow. Ref.1
Developmental stage	In early embryos expression is very low, expression increases during embryogenesis. Also expressed in larvae and pupae. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Traceable author statement PubMed 12147138. Source: FlyBase actin filament bundle assembly Inferred from mutant phenotype PubMed 16831834. Source: FlyBase adherens junction organization Inferred from mutant phenotype PubMed 18305002. Source: FlyBase apoptotic cell clearance Inferred from mutant phenotype PubMed 18432193. Source: FlyBase compound eye development Inferred from mutant phenotype Ref.1. Source: FlyBase dorsal closure, spreading of leading edge cells Inferred from mutant phenotype PubMed 15857910. Source: FlyBase epithelial cell-cell adhesion Inferred from mutant phenotype PubMed 18305002. Source: FlyBase imaginal disc fusion, thorax closure Inferred from mutant phenotype PubMed 15857910. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 16648592. Source: FlyBase negative regulation of epidermal growth factor receptor signaling pathway Inferred from genetic interaction PubMed 10075855. Source: FlyBase negative regulation of synaptic growth at neuromuscular junction Inferred from genetic interaction PubMed 18925939. Source: FlyBase open tracheal system development Inferred from mutant phenotype PubMed 16469972PubMed 18305002. Source: FlyBase peptidyl-tyrosine phosphorylation Inferred from mutant phenotype PubMed 12014990. Source: FlyBase salivary gland morphogenesis Inferred from mutant phenotype PubMed 17507403. Source: FlyBase transmembrane receptor protein tyrosine kinase signaling pathway Inferred from genetic interaction PubMed 10075855. Source: FlyBase tricarboxylic acid cycle Inferred from direct assay PubMed 18042644. Source: FlyBase
Cellular_component	adherens junction Inferred from direct assay PubMed 18305002. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Tyrosine-protein kinase Src42A		PRO_0000088139
Regions
	Domain	63 – 124	62	SH3
	Domain	130 – 222	93	SH2
	Domain	248 – 504	257	Protein kinase
	Nucleotide binding	254 – 262	9	ATP By similarity UniProtKB P08631
Sites
	Active site	370	1	Proton acceptor By similarity UniProtKB P08631
	Binding site	276	1	ATP By similarity UniProtKB P08631
Experimental info
	Sequence conflict	65	1	A → V in BAA07705. Ref.1
	Sequence conflict	376	1	V → I in AAB19907. Ref.5
	Sequence conflict	376	1	V → I in CAA05746. Ref.6
	Sequence conflict	381 – 386	6	GNIVKI → SNVVKM in AAB19907. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9V9J3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1EF196E4D7AE61E9	FASTA	517	59,069
	10 20 30 40 50 60 MGNCLTTQKG EPDKPADRIK LDDPPTIGVG VGVPQIPMPS HAGQPPEQIR PVPQIPESET 70 80 90 100 110 120 AGANAKIFVA LYDYDARTDE DLSFRKGEHL EILNDTQGDW WLARSKKTRS EGYIPSNYVA 130 140 150 160 170 180 KLKSIEAEPW YFRKIKRIEA EKKLLLPENE HGAFLIRDSE SRHNDYSLSV RDGDTVKHYR 190 200 210 220 230 240 IRQLDEGGFF IARRTTFRTL QELVEHYSKD SDGLCVNLCK PCVQIEKPVT EGLSHRTRDQ 250 260 270 280 290 300 WEIDRTSLKF VRKLGSGQFG DVWEGLWNNT TPVAIKTLKS GTMDPKDFLA EAQIMKKLRH 310 320 330 340 350 360 TKLIQLYAVC TVEEPIYIIT ELMKHGSLLE YLQAIAGKGR SLKMQTLIDM AAQIAAGMAY 370 380 390 400 410 420 LESQNYIHRD LAARNVLVGD GNIVKIADFG LARLIKEDEY EARVGARFPI KWTAPEAANY 430 440 450 460 470 480 SKFSIKSDVW SFGILLTELV TYGRIPYPGM TNAEVLTQVE HGYRMPQPPN CEPRLYEIML 490 500 510 ECWHKDPMRR PTFETLQWKL EDFYTSDQSD YKEAQAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src." Takahashi F., Endo S., Kojima T., Saigo K. Genes Dev. 10:1645-1656(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S. Tissue: Pupae.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Identification of seven novel protein-tyrosine kinase genes of Drosophila by the polymerase chain reaction." Shishido E., Emori Y., Saigo K. FEBS Lett. 289:235-238(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-428.
[6]	"Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA." Oates A.C., Wollberg P., Achen M.G., Wilks A.F. Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 376-427. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D42125 mRNA. Translation: BAA07705.1. AE013599 Genomic DNA. Translation: AAF57295.1. AY058652 mRNA. Translation: AAL13881.1. S55977 Genomic DNA. Translation: AAB19907.1. AJ002911 Genomic DNA. Translation: CAA05746.1.
RefSeq	NP_476849.1. NM_057501.4. NP_724441.1. NM_165452.1.
UniGene	Dm.4134.
3D structure databases
ProteinModelPortal	Q9V9J3.
SMR	Q9V9J3. Positions 37-515.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-22220N.
IntAct	Q9V9J3. 2 interactions.
MINT	MINT-333459.
STRING	7227.FBpp0085321.
Proteomic databases
PaxDb	Q9V9J3.
PRIDE	Q9V9J3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085967; FBpp0085320; FBgn0004603. FBtr0085968; FBpp0085321; FBgn0004603.
GeneID	35524.
KEGG	dme:Dmel_CG7873.
Organism-specific databases
CTD	35524.
FlyBase	FBgn0004603. Src42A.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00620000087702.
InParanoid	Q9V9J3.
KO	K08892.
OMA	SHYTKTS.
OrthoDB	EOG4KSN11.
PhylomeDB	Q9V9J3.
Enzyme and pathway databases
BRENDA	2.7.10.2. 1994.
Gene expression databases
Bgee	Q9V9J3.
GermOnline	CG7873. Drosophila melanogaster.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Src42A. drosophila.
GenomeRNAi	35524.
NextBio	793845.

Entry information

Entry name

SRC42_DROME

Accession

Primary (citable) accession number: Q9V9J3
Secondary accession number(s): O18369 , Q0E9P0, Q26297, Q94879

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project