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Protein

Tyrosine-protein kinase Src42A

Gene

Src42A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required directly or indirectly for the phosphorylation of drpr which is necessary for the interaction of drpr with shark and subsequent glial phagocytic activity (PubMed:18432193). Together with drpr and shark, promotes the migration of macrophages to sites of wounding as part of a signaling cascade where Scr42a detects production of hydrogen peroxide at wound sites which triggers phosphorylation of drpr and subsequent recruitment and activation of shark (PubMed:26028435). Essential for correct eye morphogenesis (ommatidial R7 neuron formation) which requires the Ras1/MAPK signal transduction pathway (PubMed:8682295). May be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia (PubMed:8682295). During embryogenesis, involved in regulation of dorsal closure where it may have a role in activating the JNK pathway in leading edge cells during this process (PubMed:16831834).4 Publications

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei276ATPPROSITE-ProRule annotation1
Active sitei370Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi254 – 262ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: FlyBase
  • adherens junction organization Source: FlyBase
  • apoptotic cell clearance Source: FlyBase
  • axon guidance Source: FlyBase
  • cell migration Source: FlyBase
  • compound eye development Source: FlyBase
  • defense response to bacterium Source: FlyBase
  • dorsal closure Source: FlyBase
  • dorsal closure, spreading of leading edge cells Source: FlyBase
  • epithelial cell-cell adhesion Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • imaginal disc fusion, thorax closure Source: FlyBase
  • innate immune response Source: GO_Central
  • intestinal stem cell homeostasis Source: FlyBase
  • JNK cascade Source: FlyBase
  • negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • open tracheal system development Source: FlyBase
  • peptidyl-tyrosine autophosphorylation Source: GO_Central
  • peptidyl-tyrosine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell proliferation Source: GO_Central
  • regulation of synaptic plasticity Source: FlyBase
  • salivary gland morphogenesis Source: FlyBase
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: FlyBase
  • tricarboxylic acid cycle Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 1994
ReactomeiR-DME-432553 Phosphorylation of PER and TIM
R-DME-6798695 Neutrophil degranulation
R-DME-8847993 ERBB2 Activates PTK6 Signaling
R-DME-8849468 PTK6 Regulates Proteins Involved in RNA Processing
R-DME-8849469 PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1
R-DME-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases
R-DME-8849472 PTK6 Down-Regulation
R-DME-8849474 PTK6 Activates STAT3
R-DME-8948751 Regulation of PTEN stability and activity
SignaLinkiQ9V9J3

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Src42A (EC:2.7.10.2)
Alternative name(s):
Tyrosine-protein kinase Src41
Short name:
Dsrc41
Gene namesi
Name:Src42AImported
Synonyms:Src41, TK5Imported
ORF Names:CG44128Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0264959 Src42A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Following epithelial wounding, no migration of macrophages to wound sites (PubMed:26028435). RNAi-mediated knockdown in glial cells potently suppresses glial phagocytic activity with drpr not recruited to severed maxillary palp axons, blockage of glial hypertrophy, blockage of drpr up-regulation after antennal ablation and reduced clearance of severed axons in the central nervous system (PubMed:18432193).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → D in Src42A-myri; 63% of mutants die during embryogenesis. In embryos, leading edge cell morphology is irregular, the actomyosin cable is disrupted and dorsal closure is delayed. Stage 16 embryos, which have undergone dorsal closure, have an irregular arrangement of epidermal cells. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881391 – 517Tyrosine-protein kinase Src42AAdd BLAST517

Proteomic databases

PaxDbiQ9V9J3
PRIDEiQ9V9J3

Expressioni

Tissue specificityi

Ubiquitous in early embryos, in stages 13-16 expression is seen in visceral mesoderm, hindgut, brain, anal pads and ventral ganglions. In larvae, expression is in CNS, wing disk, leg disk and photoreceptor precursors in the eye-antenna disks posterior to the morphogenetic furrow.1 Publication

Developmental stagei

In early embryos expression is very low, expression increases during embryogenesis. Also expressed in larvae and pupae.1 Publication

Gene expression databases

BgeeiFBgn0264959
ExpressionAtlasiQ9V9J3 baseline and differential
GenevisibleiQ9V9J3 DM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi61430, 51 interactors
DIPiDIP-22220N
IntActiQ9V9J3, 3 interactors
MINTiQ9V9J3
STRINGi7227.FBpp0085320

Structurei

3D structure databases

ProteinModelPortaliQ9V9J3
SMRiQ9V9J3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 124SH3PROSITE-ProRule annotationCuratedAdd BLAST62
Domaini130 – 222SH2PROSITE-ProRule annotationCuratedAdd BLAST93
Domaini248 – 504Protein kinasePROSITE-ProRule annotationAdd BLAST257

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
GeneTreeiENSGT00760000118938
InParanoidiQ9V9J3
KOiK08892
OMAiSDGLCVK
OrthoDBiEOG091G0D46
PhylomeDBiQ9V9J3

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 1 hit
PF00017 SH2, 1 hit
PF00018 SH3_1, 1 hit
PRINTSiPR00401 SH2DOMAIN
PR00452 SH3DOMAIN
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00326 SH3, 1 hit
SM00219 TyrKc, 1 hit
SUPFAMiSSF50044 SSF50044, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V9J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCLTTQKG EPDKPADRIK LDDPPTIGVG VGVPQIPMPS HAGQPPEQIR
60 70 80 90 100
PVPQIPESET AGANAKIFVA LYDYDARTDE DLSFRKGEHL EILNDTQGDW
110 120 130 140 150
WLARSKKTRS EGYIPSNYVA KLKSIEAEPW YFRKIKRIEA EKKLLLPENE
160 170 180 190 200
HGAFLIRDSE SRHNDYSLSV RDGDTVKHYR IRQLDEGGFF IARRTTFRTL
210 220 230 240 250
QELVEHYSKD SDGLCVNLCK PCVQIEKPVT EGLSHRTRDQ WEIDRTSLKF
260 270 280 290 300
VRKLGSGQFG DVWEGLWNNT TPVAIKTLKS GTMDPKDFLA EAQIMKKLRH
310 320 330 340 350
TKLIQLYAVC TVEEPIYIIT ELMKHGSLLE YLQAIAGKGR SLKMQTLIDM
360 370 380 390 400
AAQIAAGMAY LESQNYIHRD LAARNVLVGD GNIVKIADFG LARLIKEDEY
410 420 430 440 450
EARVGARFPI KWTAPEAANY SKFSIKSDVW SFGILLTELV TYGRIPYPGM
460 470 480 490 500
TNAEVLTQVE HGYRMPQPPN CEPRLYEIML ECWHKDPMRR PTFETLQWKL
510
EDFYTSDQSD YKEAQAY
Length:517
Mass (Da):59,069
Last modified:May 1, 2000 - v1
Checksum:i1EF196E4D7AE61E9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65A → V in BAA07705 (PubMed:8682295).Curated1
Sequence conflicti376V → I in AAB19907 (PubMed:1915852).Curated1
Sequence conflicti376V → I in CAA05746 (PubMed:9731193).Curated1
Sequence conflicti381 – 386GNIVKI → SNVVKM in AAB19907 (PubMed:1915852).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42125 mRNA Translation: BAA07705.1
AE013599 Genomic DNA Translation: AAF57295.1
AY058652 mRNA Translation: AAL13881.1
S55977 Genomic DNA Translation: AAB19907.1
AJ002911 Genomic DNA Translation: CAA05746.1
RefSeqiNP_476849.1, NM_057501.4
UniGeneiDm.4134

Genome annotation databases

EnsemblMetazoaiFBtr0335275; FBpp0307254; FBgn0264959
GeneIDi35524
KEGGidme:Dmel_CG44128

Similar proteinsi

Entry informationi

Entry nameiSRC42_DROME
AccessioniPrimary (citable) accession number: Q9V9J3
Secondary accession number(s): O18369
, Q0E9P0, Q26297, Q94879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2003
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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