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Protein

Tyrosine-protein kinase Src42A

Gene

Src42A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for correct eye morphogenesis (ommatidial R7 neuron formation), this requires the Ras1/MAPK signal transduction pathway. May be involved in the regulation of cytoskeleton organization and cell-cell contacts in developing ommatidia.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei276 – 2761ATPPROSITE-ProRule annotation
Active sitei370 – 3701Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi254 – 2629ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: GO_Central
  3. protein tyrosine kinase activity Source: UniProtKB
  4. receptor binding Source: GO_Central

GO - Biological processi

  1. actin filament bundle assembly Source: FlyBase
  2. adherens junction organization Source: FlyBase
  3. apoptotic cell clearance Source: FlyBase
  4. axon guidance Source: FlyBase
  5. cell differentiation Source: GO_Central
  6. cell migration Source: FlyBase
  7. compound eye development Source: FlyBase
  8. dorsal closure Source: FlyBase
  9. dorsal closure, spreading of leading edge cells Source: FlyBase
  10. epithelial cell-cell adhesion Source: FlyBase
  11. imaginal disc-derived wing morphogenesis Source: FlyBase
  12. imaginal disc fusion, thorax closure Source: FlyBase
  13. innate immune response Source: GO_Central
  14. JNK cascade Source: FlyBase
  15. negative regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
  16. negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  17. open tracheal system development Source: FlyBase
  18. peptidyl-tyrosine autophosphorylation Source: GO_Central
  19. peptidyl-tyrosine phosphorylation Source: FlyBase
  20. protein phosphorylation Source: FlyBase
  21. regulation of cell proliferation Source: GO_Central
  22. salivary gland morphogenesis Source: FlyBase
  23. transmembrane receptor protein tyrosine kinase signaling pathway Source: FlyBase
  24. tricarboxylic acid cycle Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
SignaLinkiQ9V9J3.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase Src42A (EC:2.7.10.2)
Alternative name(s):
Tyrosine-protein kinase Src41
Short name:
Dsrc41
Gene namesi
Name:Src42A
Synonyms:Src41, TK5
ORF Names:CG44128
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0264959. Src42A.

Subcellular locationi

GO - Cellular componenti

  1. adherens junction Source: FlyBase
  2. cytoplasm Source: FlyBase
  3. extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
  4. plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Tyrosine-protein kinase Src42APRO_0000088139Add
BLAST

Proteomic databases

PaxDbiQ9V9J3.
PRIDEiQ9V9J3.

Expressioni

Tissue specificityi

Ubiquitous in early embryos, in stages 13-16 expression is seen in visceral mesoderm, hindgut, brain, anal pads and ventral ganglions. In larvae, expression is in CNS, wing disk, leg disk and photoreceptor precursors in the eye-antenna disks posterior to the morphogenetic furrow.1 Publication

Developmental stagei

In early embryos expression is very low, expression increases during embryogenesis. Also expressed in larvae and pupae.1 Publication

Gene expression databases

BgeeiQ9V9J3.
ExpressionAtlasiQ9V9J3. differential.

Interactioni

Protein-protein interaction databases

BioGridi61430. 27 interactions.
DIPiDIP-22220N.
IntActiQ9V9J3. 2 interactions.
MINTiMINT-333459.
STRINGi7227.FBpp0085321.

Structurei

3D structure databases

ProteinModelPortaliQ9V9J3.
SMRiQ9V9J3. Positions 37-515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 12462SH3PROSITE-ProRule annotationCuratedAdd
BLAST
Domaini130 – 22293SH2PROSITE-ProRule annotationCuratedAdd
BLAST
Domaini248 – 504257Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotationCurated
Contains 1 SH3 domain.PROSITE-ProRule annotationCurated

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ9V9J3.
KOiK08892.
OMAiKNLRHPK.
PhylomeDBiQ9V9J3.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V9J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCLTTQKG EPDKPADRIK LDDPPTIGVG VGVPQIPMPS HAGQPPEQIR
60 70 80 90 100
PVPQIPESET AGANAKIFVA LYDYDARTDE DLSFRKGEHL EILNDTQGDW
110 120 130 140 150
WLARSKKTRS EGYIPSNYVA KLKSIEAEPW YFRKIKRIEA EKKLLLPENE
160 170 180 190 200
HGAFLIRDSE SRHNDYSLSV RDGDTVKHYR IRQLDEGGFF IARRTTFRTL
210 220 230 240 250
QELVEHYSKD SDGLCVNLCK PCVQIEKPVT EGLSHRTRDQ WEIDRTSLKF
260 270 280 290 300
VRKLGSGQFG DVWEGLWNNT TPVAIKTLKS GTMDPKDFLA EAQIMKKLRH
310 320 330 340 350
TKLIQLYAVC TVEEPIYIIT ELMKHGSLLE YLQAIAGKGR SLKMQTLIDM
360 370 380 390 400
AAQIAAGMAY LESQNYIHRD LAARNVLVGD GNIVKIADFG LARLIKEDEY
410 420 430 440 450
EARVGARFPI KWTAPEAANY SKFSIKSDVW SFGILLTELV TYGRIPYPGM
460 470 480 490 500
TNAEVLTQVE HGYRMPQPPN CEPRLYEIML ECWHKDPMRR PTFETLQWKL
510
EDFYTSDQSD YKEAQAY
Length:517
Mass (Da):59,069
Last modified:April 30, 2000 - v1
Checksum:i1EF196E4D7AE61E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651A → V in BAA07705 (PubMed:8682295).Curated
Sequence conflicti376 – 3761V → I in AAB19907 (PubMed:1915852).Curated
Sequence conflicti376 – 3761V → I in CAA05746 (PubMed:9731193).Curated
Sequence conflicti381 – 3866GNIVKI → SNVVKM in AAB19907 (PubMed:1915852).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42125 mRNA. Translation: BAA07705.1.
AE013599 Genomic DNA. Translation: AAF57295.1.
AY058652 mRNA. Translation: AAL13881.1.
S55977 Genomic DNA. Translation: AAB19907.1.
AJ002911 Genomic DNA. Translation: CAA05746.1.
RefSeqiNP_476849.1. NM_057501.4.
UniGeneiDm.4134.

Genome annotation databases

EnsemblMetazoaiFBtr0335275; FBpp0307254; FBgn0264959.
GeneIDi35524.
KEGGidme:Dmel_CG44128.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42125 mRNA. Translation: BAA07705.1.
AE013599 Genomic DNA. Translation: AAF57295.1.
AY058652 mRNA. Translation: AAL13881.1.
S55977 Genomic DNA. Translation: AAB19907.1.
AJ002911 Genomic DNA. Translation: CAA05746.1.
RefSeqiNP_476849.1. NM_057501.4.
UniGeneiDm.4134.

3D structure databases

ProteinModelPortaliQ9V9J3.
SMRiQ9V9J3. Positions 37-515.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61430. 27 interactions.
DIPiDIP-22220N.
IntActiQ9V9J3. 2 interactions.
MINTiMINT-333459.
STRINGi7227.FBpp0085321.

Proteomic databases

PaxDbiQ9V9J3.
PRIDEiQ9V9J3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0335275; FBpp0307254; FBgn0264959.
GeneIDi35524.
KEGGidme:Dmel_CG44128.

Organism-specific databases

CTDi35524.
FlyBaseiFBgn0264959. Src42A.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiQ9V9J3.
KOiK08892.
OMAiKNLRHPK.
PhylomeDBiQ9V9J3.

Enzyme and pathway databases

BRENDAi2.7.10.2. 1994.
SignaLinkiQ9V9J3.

Miscellaneous databases

ChiTaRSiSrc42A. fly.
GenomeRNAii35524.
NextBioi793845.

Gene expression databases

BgeeiQ9V9J3.
ExpressionAtlasiQ9V9J3. differential.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src."
    Takahashi F., Endo S., Kojima T., Saigo K.
    Genes Dev. 10:1645-1656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
    Tissue: Pupae.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Identification of seven novel protein-tyrosine kinase genes of Drosophila by the polymerase chain reaction."
    Shishido E., Emori Y., Saigo K.
    FEBS Lett. 289:235-238(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-428.
  6. "Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA."
    Oates A.C., Wollberg P., Achen M.G., Wilks A.F.
    Biochem. Biophys. Res. Commun. 249:660-667(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 376-427.
    Tissue: Embryo.

Entry informationi

Entry nameiSRC42_DROME
AccessioniPrimary (citable) accession number: Q9V9J3
Secondary accession number(s): O18369
, Q0E9P0, Q26297, Q94879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2003
Last sequence update: April 30, 2000
Last modified: March 3, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.