Reviewed, UniProtKB/Swiss-Prot Q9V8M5 (3HIDH_DROME)

Names and origin

Protein names

Recommended name:
    Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
      Short name=HIBADH
    EC=1.1.1.31

Gene names

ORF Names:

CG15093

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lipid particle Inferred from direct assay. Source: FlyBase mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W1K6	1	EBI-241464,EBI-178006

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9V8M5-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform C (identifier: Q9V8M5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     95-227: NAIVDASYDE...VRQGLDANVF → KCRQGRPRVH...GAQGLPVAVR
     228-324: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion By similarity
	Chain	26 – 324	299	Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial		PRO_0000007161
Regions
	Nucleotide binding	29 – 58	30	NAD By similarity
	Nucleotide binding	92 – 93	2	NAD By similarity
Sites
	Active site	196	1	By similarity
	Binding site	121	1	NAD; via amide nitrogen By similarity
	Binding site	271	1	NAD By similarity
Natural variations
	Alternative sequence	95 – 227	133	NAIVD…DANVF → KCRQGRPRVHGQEDHPLRRL WHGPGRQAVQQHDAGHLDDR CFGGHESGGAPGSRCQCLRR DHQLLHRTLLGLGDLQPCAR SLPQCPSQQGLRRRFLLGSD HQGSGSGLRSGQRFQLTHPA GISGAQGLPVAVR in isoform C.		VSP_001281
	Alternative sequence	228 – 324	97	Missing in isoform C.		VSP_001282

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (B) [UniParc]. Last modified January 24, 2001. Version 2. Checksum: A39B534753EAE83E	FASTA	324	33,883
	10 20 30 40 50 60 MSLRVMSPAM LNAWSQTLVR AMSTQGGAKN IGFVGLGNMG ANMASNLIKA GHKLHVFDIS 70 80 90 100 110 120 KPACDGLAAK GATVYAKTSE LAKNSDFVIT MLPNNAIVDA SYDEMTADGV NKDTIFIDSS 130 140 150 160 170 180 TISPDLVKSL QKKISAKGAR FIDAPVSGGV PGAEQATLTF MVGGTEAEYN AVKAVLECMG 190 200 210 220 230 240 KKITHCGVYG MGQAAKLCNN MMLAISMIGV SEAMNLAVRQ GLDANVFAEI INSSTGRCWA 250 260 270 280 290 300 SEIYNPVPGV CPSAPANRDY AGGFSSALIT KDLGLASGVA NASNSPIPLG SLAHKVYQSL 310 320 CDKGLGNKDF SVVYDLMKKE KFSV
	Isoform C. Checksum: 15BD483B4513E55D	FASTA	227	24,373
	10 20 30 40 50 60 MSLRVMSPAM LNAWSQTLVR AMSTQGGAKN IGFVGLGNMG ANMASNLIKA GHKLHVFDIS 70 80 90 100 110 120 KPACDGLAAK GATVYAKTSE LAKNSDFVIT MLPNKCRQGR PRVHGQEDHP LRRLWHGPGR 130 140 150 160 170 180 QAVQQHDAGH LDDRCFGGHE SGGAPGSRCQ CLRRDHQLLH RTLLGLGDLQ PCARSLPQCP 190 200 210 220 SQQGLRRRFL LGSDHQGSGS GLRSGQRFQL THPAGISGAQ GLPVAVR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF57638.2. AE013599 Genomic DNA. Translation: AAF57639.2. AY069057 mRNA. Translation: AAL39202.2. Different initiation.
RefSeq	NP_611373.1. NP_725824.1. NP_725825.1.
UniGene	Dm.21583
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22109N.
IntAct	Q9V8M5. 1 interaction.
Genome annotation databases
Ensembl	FBgn0034390. Drosophila melanogaster. [Contig view]
GeneID	37166.
KEGG	dme:Dmel_CG15093.
Organism-specific databases
FlyBase	FBgn0034390. CG15093.
Phylogenomic databases
HOGENOM	Q9V8M5.
OMA	Q9V8M5. PMARNLI.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005425-MON. DMEL-XXX-02:DMEL-XXX-02-005426-MON.
BRENDA	1.1.1.31. 48.
Gene expression databases
ArrayExpress	Q9V8M5.
GermOnline	CG15093. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR006183. 6-phosphogluconate_DH. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00076. 6PGDHDRGNASE.
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	802277.

Entry information

Entry name

3HIDH_DROME

Accession

Primary (citable) accession number: Q9V8M5
Secondary accession number(s): Q0E926, Q86R98, Q9V8M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	January 24, 2001
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project