Reviewed, UniProtKB/Swiss-Prot Q9V7U0 (RESIL_DROME)

Names and origin

Protein names

Recommended name:
    Pro-resilin

Gene names

Name:	resilin
ORF Names:	CG15920

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a central role in insect flight by providing low stiffness, high strain and efficient energy storage Probable.
Developmental stage	Expressed at pupal stage only. Ref.5
Biotechnological use	Its strong elasticity suggests that it may be used for industrial and pharmaceutical applications. Recombinant protein can be cast into a rubber-like biomaterial by rapid photochemical cross-linking. The resilience (recovery after deformation) of cross-linked recombinant resilin exceeds that of unfilled synthetic polybutadiene, a high resilience rubber. Ref.5
Sequence similarities	Contains 1 chitin-binding type R&R domain.

Ontologies

Keywords
Cellular component	Cuticle
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Ref.4 Non-traceable author statement. Source: UniProtKB
Molecular function	extracellular matrix constituent conferring elasticity Ref.4 Non-traceable author statement. Source: UniProtKB structural constituent of cuticle Ref.4 Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A Ref.1 (identifier: Q9V7U0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform B Ref.1 (identifier: Q9V7U0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     341-385: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 620	603	Pro-resilin		PRO_0000042845
Regions
	Repeat	42 – 56	15	Elastic 1
	Repeat	57 – 77	21	Elastic 2
	Repeat	78 – 97	20	Elastic 3
	Repeat	98 – 112	15	Elastic 4
	Repeat	113 – 127	15	Elastic 5
	Repeat	128 – 145	18	Elastic 6
	Repeat	146 – 164	19	Elastic 7
	Repeat	165 – 179	15	Elastic 8
	Repeat	180 – 194	15	Elastic 9
	Repeat	195 – 210	16	Elastic 10
	Repeat	211 – 225	15	Elastic 11
	Repeat	226 – 244	19	Elastic 12
	Repeat	245 – 262	18	Elastic 13
	Repeat	263 – 276	14	Elastic 14
	Repeat	277 – 292	16	Elastic 15
	Repeat	293 – 307	15	Elastic 16
	Repeat	308 – 324	17	Elastic 17
	Domain	342 – 415	74	Chitin-binding type R&R
	Compositional bias	34 – 617	584	Gly-rich
	Compositional bias	168 – 171	4	Poly-Ser
Natural variations
	Alternative sequence	341 – 385	45	Missing in isoform B. Ref.1		VSP_051872

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 432AFA06E54CB6BA	FASTA	620	58,604
	10 20 30 40 50 60 MFKLLGLTLL MAMVVLGRPE PPVNSYLPPS DSYGAPGQSG PGGRPSDSYG APGGGNGGRP 70 80 90 100 110 120 SDSYGAPGQG QGQGQGQGGY AGKPSDTYGA PGGGNGNGGR PSSSYGAPGG GNGGRPSDTY 130 140 150 160 170 180 GAPGGGNGGR PSDTYGAPGG GGNGNGGRPS SSYGAPGQGQ GNGNGGRSSS SYGAPGGGNG 190 200 210 220 230 240 GRPSDTYGAP GGGNGGRPSD TYGAPGGGNN GGRPSSSYGA PGGGNGGRPS DTYGAPGGGN 250 260 270 280 290 300 GNGSGGRPSS SYGAPGQGQG GFGGRPSDSY GAPGQNQKPS DSYGAPGSGN GNGGRPSSSY 310 320 330 340 350 360 GAPGSGPGGR PSDSYGPPAS GSGAGGAGGS GPGGADYDND EPAKYEFNYQ VEDAPSGLSF 370 380 390 400 410 420 GHSEMRDGDF TTGQYNVLLP DGRKQIVEYE ADQQGYRPQI RYEGDANDGS GPSGPGGPGG 430 440 450 460 470 480 QNLGADGYSS GRPGNGNGNG NGGYSGGRPG GQDLGPSGYS GGRPGGQDLG AGGYSNGKPG 490 500 510 520 530 540 GQDLGPGGYS GGRPGGQDLG RDGYSGGRPG GQDLGASGYS NGRPGGNGNG GSDGGRVIIG 550 560 570 580 590 600 GRVIGGQDGG DQGYSGGRPG GQDLGRDGYS SGRPGGRPGG NGQDSQDGQG YSSGRPGQGG 610 620 RNGFGPGGQN GDNDGSGYRY
	Isoform B. Checksum: D234139F8273427D	FASTA	575	53,526
	10 20 30 40 50 60 MFKLLGLTLL MAMVVLGRPE PPVNSYLPPS DSYGAPGQSG PGGRPSDSYG APGGGNGGRP 70 80 90 100 110 120 SDSYGAPGQG QGQGQGQGGY AGKPSDTYGA PGGGNGNGGR PSSSYGAPGG GNGGRPSDTY 130 140 150 160 170 180 GAPGGGNGGR PSDTYGAPGG GGNGNGGRPS SSYGAPGQGQ GNGNGGRSSS SYGAPGGGNG 190 200 210 220 230 240 GRPSDTYGAP GGGNGGRPSD TYGAPGGGNN GGRPSSSYGA PGGGNGGRPS DTYGAPGGGN 250 260 270 280 290 300 GNGSGGRPSS SYGAPGQGQG GFGGRPSDSY GAPGQNQKPS DSYGAPGSGN GNGGRPSSSY 310 320 330 340 350 360 GAPGSGPGGR PSDSYGPPAS GSGAGGAGGS GPGGADYDND IVEYEADQQG YRPQIRYEGD 370 380 390 400 410 420 ANDGSGPSGP GGPGGQNLGA DGYSSGRPGN GNGNGNGGYS GGRPGGQDLG PSGYSGGRPG 430 440 450 460 470 480 GQDLGAGGYS NGKPGGQDLG PGGYSGGRPG GQDLGRDGYS GGRPGGQDLG ASGYSNGRPG 490 500 510 520 530 540 GNGNGGSDGG RVIIGGRVIG GQDGGDQGYS GGRPGGQDLG RDGYSSGRPG GRPGGNGQDS 550 560 570 QDGQGYSSGR PGQGGRNGFG PGGQNGDNDG SGYRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Strain: Berkeley. Tissue: Testis.
[4]	"Tentative identification of a resilin gene in Drosophila melanogaster." Ardell D.H., Andersen S.O. Insect Biochem. Mol. Biol. 31:965-970(2001) [PubMed: 11483432] [Abstract] Cited for: IDENTIFICATION.
[5]	"Synthesis and properties of crosslinked recombinant pro-resilin." Elvin C.M., Carr A.G., Huson M.G., Maxwell J.M., Pearson R.D., Vuocolo T., Liyou N.E., Wong D.C.C., Merritt D.J., Dixon N.E. Nature 437:999-1002(2005) [PubMed: 16222249] [Abstract] Cited for: DEVELOPMENTAL STAGE, BIOTECHNOLOGICAL RELEVANCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF57953.1. AE013599 Genomic DNA. Translation: AAS64829.1. BT003322 mRNA. Translation: AAO25082.1.
RefSeq	NP_611157.1. NP_995860.1.
UniGene	Dm.19192
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9V7U0. 1 interaction.
Genome annotation databases
Ensembl	FBgn0034157. Drosophila melanogaster. [Contig view]
GeneID	36880.
KEGG	dme:Dmel_CG15920.
NMPDR	fig|7227.3.peg.5819.
Organism-specific databases
FlyBase	FBgn0034157. resilin.
Phylogenomic databases
HOGENOM	Q9V7U0.
OMA	Q9V7U0. NGGRPSD.
Gene expression databases
ArrayExpress	Q9V7U0.
GermOnline	CG15920. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000618. Insect_cuticle. [Graphical view]
Pfam	PF00379. Chitin_bind_4. 1 hit. [Graphical view]
PROSITE	PS00233. CHIT_BIND_RR_1. 1 hit. PS51155. CHIT_BIND_RR_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	800852.

Entry information

Entry name

RESIL_DROME

Accession

Primary (citable) accession number: Q9V7U0
Secondary accession number(s): Q86P83

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project