Reviewed, UniProtKB/Swiss-Prot Q9V7G5 (C4AA1_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 4aa1
    EC=1.14.-.-
Alternative name(s):
    CYPIVAA1

Gene names

Name:	Cyp4aa1
ORF Names:	CG8302

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Potential. Microsome membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: Q9V7G5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A (identifier: Q9V7G5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     348-375: DSNRAPTMTDLHEMRYMEMCIKEALRLY → FLWGIYIYFLLKQCYICFICNKVMLVGI
     376-510: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Probable cytochrome P450 4aa1		PRO_0000051825
Sites
	Metal binding	450	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	348 – 375	28	DSNRA…ALRLY → FLWGIYIYFLLKQCYICFIC NKVMLVGI in isoform A.		VSP_036909
	Alternative sequence	376 – 510	135	Missing in isoform A.		VSP_036910

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 91FD50E2E325D3DF	FASTA	510	58,536
	10 20 30 40 50 60 MFVEKVLERT TNLELCSILI LLVISLSIYT FYATLNTYLR SVLLSLRLTG PPSLPFLGNC 70 80 90 100 110 120 MLVTDKDLMR RCAGKAFDLY GSLVRIWVLL FPFFAVLEPE DLQVILSSKK HTNKVFFYRL 130 140 150 160 170 180 MHNFLGDGLI TSSGSKWSNH RRLIQPAFHH NLLEKFIDTF VDASQSLYEN LDAEAVGTEI 190 200 210 220 230 240 NIAKYVNNCV LDILNEAVLG VPIKKRGQDV AMMEDSPFRQ GKIMMPARFT QPWLLLDGIY 250 260 270 280 290 300 HWTKMANDEL NQKKRLNDFT RKMIQRRRQI QNNNNGNSER KCLLDHMIEI SESNRDFTEE 310 320 330 340 350 360 DIVNEACTFM LAGQDSVGAA VAFTLFLLTQ NPECQDRCVL ELATIFEDSN RAPTMTDLHE 370 380 390 400 410 420 MRYMEMCIKE ALRLYPSVPL IARKLGEEVR LAKHTLPAGS NVFICPYATH RLAHIYPDPE 430 440 450 460 470 480 KFQPERFSPE NSENRHPYAF LPFSAGPRYC IGNRFAIMEI KTIVSRLLRS YQLLPVTGKT 490 500 510 TIAATFRITL RASGGLWVRL KERDHPLIAH
	Isoform A. Checksum: 35AA64CEA33DC42C	FASTA	375	43,223
	10 20 30 40 50 60 MFVEKVLERT TNLELCSILI LLVISLSIYT FYATLNTYLR SVLLSLRLTG PPSLPFLGNC 70 80 90 100 110 120 MLVTDKDLMR RCAGKAFDLY GSLVRIWVLL FPFFAVLEPE DLQVILSSKK HTNKVFFYRL 130 140 150 160 170 180 MHNFLGDGLI TSSGSKWSNH RRLIQPAFHH NLLEKFIDTF VDASQSLYEN LDAEAVGTEI 190 200 210 220 230 240 NIAKYVNNCV LDILNEAVLG VPIKKRGQDV AMMEDSPFRQ GKIMMPARFT QPWLLLDGIY 250 260 270 280 290 300 HWTKMANDEL NQKKRLNDFT RKMIQRRRQI QNNNNGNSER KCLLDHMIEI SESNRDFTEE 310 320 330 340 350 360 DIVNEACTFM LAGQDSVGAA VAFTLFLLTQ NPECQDRCVL ELATIFEFLW GIYIYFLLKQ 370 CYICFICNKV MLVGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF58091.2. BT025154 mRNA. Translation: ABE73324.1.
RefSeq	NP_611067.2.
UniGene	Dm.33964
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0034053. Drosophila melanogaster. [Contig view]
GeneID	36752.
KEGG	dme:Dmel_CG8302.
NMPDR	fig|7227.3.peg.5467.
Organism-specific databases
FlyBase	FBgn0034053. Cyp4aa1.
Phylogenomic databases
HOGENOM	Q9V7G5.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004839-MON.
Gene expression databases
ArrayExpress	Q9V7G5.
GermOnline	CG8302. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	800197.

Entry information

Entry name

C4AA1_DROME

Accession

Primary (citable) accession number: Q9V7G5
Secondary accession number(s): Q1RKQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	April 14, 2009
Last modified:	June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project