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Protein

Exostosin-1

Gene

ttv

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. Botv is the trigger of heparan sulfate chain initiation and polymerization takes place by a complex of ttv and sotv. Plays a central role in the diffusion of morphogens hedgehog (hh), wingless (wg) and decapentaplegic (dpp) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis which are required for movement of hh, dpp and wg morphogens.9 Publications

Miscellaneous

'Tout velu' means 'very hairy' in French.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Pathwayi: heparin biosynthesis

This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi583Manganese; catalyticBy similarity1
Binding sitei611SubstrateBy similarity1
Active sitei670By similarity1

GO - Molecular functioni

GO - Biological processi

  • axon guidance Source: FlyBase
  • germ cell migration Source: FlyBase
  • glycosaminoglycan biosynthetic process Source: UniProtKB
  • heparan sulfate proteoglycan biosynthetic process Source: FlyBase
  • heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: FlyBase
  • heparin biosynthetic process Source: FlyBase
  • intracellular distribution of mitochondria Source: FlyBase
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • positive regulation of decapentaplegic signaling pathway Source: UniProtKB
  • positive regulation of smoothened signaling pathway Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway
  • regulation of smoothened signaling pathway Source: UniProtKB
  • synapse organization Source: FlyBase
  • synaptic vesicle endocytosis Source: FlyBase
  • Wnt signaling pathway Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Glycosyltransferase, Transferase
Biological processWnt signaling pathway
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.4.1.225 1994
ReactomeiR-DME-2022928 HS-GAG biosynthesis
SignaLinkiQ9V730
UniPathwayiUPA00378
UPA00756
UPA00862

Protein family/group databases

CAZyiGT47 Glycosyltransferase Family 47
GT64 Glycosyltransferase Family 64

Names & Taxonomyi

Protein namesi
Recommended name:
Exostosin-1 (EC:2.4.1.224, EC:2.4.1.225)
Alternative name(s):
Protein tout-velu
Gene namesi
Name:ttv
Synonyms:DEXT1
ORF Names:CG10117
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0265974 ttv

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST19
Topological domaini26 – 760LumenalSequence analysisAdd BLAST735

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

According to some authors (PubMed:10549295) ttv mutants have no effect on wg signaling, while according to others (PubMed:14645127, PubMed:14729575 and PubMed:15056609) wg signaling is affected. Such discrepancy may be explained by the fact that the absence of ttv could be partially compensated by the intact sotv protein.4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi447G → D in ttv205; induces defects in wing patterning due to impaired movement of morphogens. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001496641 – 760Exostosin-1Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi327N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi476N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi668 ↔ 716By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9V730
PRIDEiQ9V730

Expressioni

Tissue specificityi

Ubiquitously expressed in early embryos. Later (in stage 10 embryos), it is expressed at higher level in the nervous system. Ubiquitously expressed in wing imaginal disk.2 Publications

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0265974
ExpressionAtlasiQ9V730 baseline and differential
GenevisibleiQ9V730 DM

Interactioni

Subunit structurei

Interacts with sau (PubMed:23720043).

Binary interactionsi

WithEntry#Exp.IntActNotes
Ext2Q9Y1693EBI-166374,EBI-142791

Protein-protein interaction databases

BioGridi62362, 8 interactors
IntActiQ9V730, 5 interactors
STRINGi7227.FBpp0305367

Structurei

3D structure databases

ProteinModelPortaliQ9V730
SMRiQ9V730
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni561 – 565Substrate bindingBy similarity5
Regioni581 – 583Substrate bindingBy similarity3
Regioni666 – 670Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1021 Eukaryota
ENOG410XTFH LUCA
GeneTreeiENSGT00550000074496
InParanoidiQ9V730
KOiK02366
OMAiSHYWDDS
OrthoDBiEOG091G0BDR
PhylomeDBiQ9V730

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004263 Exostosin
IPR015338 EXT_C
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF03016 Exostosin, 1 hit
PF09258 Glyco_transf_64, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q9V730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAKKRYILV FVSCAFLAYA YFGGYRLKVS PLRPRRAQHE SAKDGGVQPH
60 70 80 90 100
EQLPSFLGAH DMQELQLLQS NQSKSLDSSK HLVTRKPDCR METCFDFTRC
110 120 130 140 150
YDRFLVYIYP PEPLNSLGAA PPTSANYQKI LTAIQESRYY TSDPTAACLF
160 170 180 190 200
VLGIDTLDRD SLSEDYVRNV PSRLARLPYW NNGRNHIIFN LYSGTWPDYA
210 220 230 240 250
ENSLGFDAGE AILAKASMGV LQLRHGFDVS IPLFHKQFPL RAGATGTVQS
260 270 280 290 300
NNFPANKKYL LAFKGKRYVH GIGSETRNSL FHLHNGRDMV LVTTCRHGKS
310 320 330 340 350
WRELQDNRCD EDNREYDRYD YETLLQNSTF CLVPRGRRLG SFRFLEALQA
360 370 380 390 400
GCIPVLLSNA WVLPFESKID WKQAAIWADE RLLLQVPDIV RSIPAERIFA
410 420 430 440 450
LRQQTQVLWE RYFGSIEKIV FTTFEIIRER LPDYPVRSSL VWNSSPGALL
460 470 480 490 500
TLPTFADSSR YMPFLLNSMG AEPRHNYTAV IYVQIGAALG PNAALYKLVR
510 520 530 540 550
TITKSQFVER ILVLWAADRP LPLKKRWPPT SHIPLHVISL GGSTRSQGAG
560 570 580 590 600
PTSQTTEGRP SISQRFLPYD EIQTDAVLSL DEDAILNTDE LDFAYTVWRD
610 620 630 640 650
FPERIVGYPA RAHFWDDSKN AWGYTSKWTN YYSIVLTGAA FYHRYYNYLY
660 670 680 690 700
TNWLSLLLLK TVQQSSNCED ILMNLLVSHV TRKPPIKVTQ RKGYKDRETG
710 720 730 740 750
RSPWNDPDHF IQRQSCLNTF AAVFGYMPLI RSNLRMDPML YRDPVSNLRK
760
KYRQIELVGS
Length:760
Mass (Da):87,309
Last modified:May 1, 2000 - v1
Checksum:iF6369F0BA206DEA9
GO

Sequence cautioni

The sequence AAC32397 differs from that shown. Reason: Frameshift at positions 694 and 702.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083889 mRNA Translation: AAC32397.1 Frameshift.
AB221351 mRNA Translation: BAE78509.1
AE013599 Genomic DNA Translation: AAF58236.1
BT021403 mRNA Translation: AAX33551.1
RefSeqiNP_001260971.1, NM_001274042.1
NP_477231.1, NM_057883.3
UniGeneiDm.2966

Genome annotation databases

EnsemblMetazoaiFBtr0087495; FBpp0086624; FBgn0265974
FBtr0333164; FBpp0305367; FBgn0265974
GeneIDi36614
KEGGidme:Dmel_CG10117

Similar proteinsi

Entry informationi

Entry nameiEXT1_DROME
AccessioniPrimary (citable) accession number: Q9V730
Secondary accession number(s): O76796, Q5BI21
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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