Reviewed, UniProtKB/Swiss-Prot Q9V6X7 (OFUT1_DROME)

Names and origin

Protein names

Recommended name:
    GDP-fucose protein O-fucosyltransferase 1
    EC=2.4.1.221
Alternative name(s):
    Peptide-O-fucosyltransferase 1
      Short name=O-FucT-1
    Neurotic protein

Gene names

Name:	O-fut1
Synonyms:	nti
ORF Names:	CG12366

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling; essential for the interaction of Notch with its ligand Delta, and for the ability of Fringe to modulate this interaction. Ref.1
Catalytic activity	Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum By similarity.
Developmental stage	Expressed both maternally and zygotically. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 68 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism Notch signaling pathway
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Notch signaling pathway Ref.7 Non-traceable author statement. Source: UniProtKB O-glycan processing Ref.7 Traceable author statement. Source: UniProtKB cell-cell adhesion Inferred from mutant phenotype. Source: FlyBase embryonic development Ref.7 Non-traceable author statement. Source: UniProtKB endocytosis Inferred from mutant phenotype. Source: FlyBase fucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of Notch signaling pathway Inferred from mutant phenotype. Source: FlyBase protein catabolic process Inferred from mutant phenotype. Source: FlyBase regulation of transcription Ref.7 Non-traceable author statement. Source: UniProtKB
Cellular component	cytoplasmic vesicle Inferred from direct assay. Source: FlyBase endoplasmic reticulum Inferred from direct assay. Source: FlyBase
Molecular function	Notch binding Inferred from direct assay. Source: FlyBase peptide-O-fucosyltransferase activity Ref.7 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9VXE7	1	EBI-97485,EBI-165741
CG12901	Q9V5K1	1	EBI-97485,EBI-113802
Synd	Q9VDI1	1	EBI-97485,EBI-106353

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 402	379	GDP-fucose protein O-fucosyltransferase 1		PRO_0000012153
Amino acid modifications
	Glycosylation	60	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	371	1	E → V in AAM50020. Ref.5
	Sequence conflict	389	1	K → E in BAC55010. Ref.1
	Sequence conflict	389	1	K → E in AAX33443. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9V6X7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E52FF4F86509C2E6	FASTA	402	46,834
	10 20 30 40 50 60 MQWLKMKLRF VNLILLLISS TCAQLGGDPN GYLTYCPCMG RFGNQADHFL GSLAFAKALN 70 80 90 100 110 120 RTLILPPWVE YRRGELRSRQ VPFNTYFEVE PLKEYHRVIT MADFMWHLAD DIWPESERVS 130 140 150 160 170 180 FCYKERYSLQ QEKNDPDKPN CHAKDGNPFG PFWDTFHIDF VRSEFYAPLH FDVHHSNEAA 190 200 210 220 230 240 KWQTKYPAES YPVLAFTGAP ASFPVQLENC KLQRYLQWSQ RYREASKDFI REQLPRGAFL 250 260 270 280 290 300 GIHLRNGIDW VRACEHVKDS QHLFASPQCL GYKNERGALY PELCMPSKEA IIRQLKRTIK 310 320 330 340 350 360 NVRQTQPDNE IKSVFVASDS NHMIGELNTA LSRMGISVHK LPEDDPYLDL AILGQSNHFI 370 380 390 400 GNCISSYSAF EKRERDVHGF PSYFWGFPKE KDRKHTNVHE EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Neurotic, a novel maternal neurogenic gene, encodes an O-fucosyltransferase that is essential for Notch-Delta interactions." Sasamura T., Sasaki N., Miyashita F., Nakao S., Ishikawa H., Ito M., Kitagawa M., Harigaya K., Spana E., Bilder D., Perrmon N., Matsuno K. Development 130:4785-4795(2003) [PubMed: 12917292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-402. Strain: Berkeley. Tissue: Embryo.
[6]	"Modification of epidermal growth factor-like repeats with O-fucose: molecular cloning and expression of a novel GDP-fucose protein O-fucosyltransferase." Wang Y., Shao L., Shi S., Harris R.J., Spellman M.W., Stanley P., Haltiwanger R.S. J. Biol. Chem. 276:40338-40345(2001) [PubMed: 11524432] [Abstract] Cited for: HOMOLOGY.
[7]	"Composition of Drosophila melanogaster proteome involved in fucosylated glycan metabolism." Roos C., Kolmer M., Mattila P., Renkonen R. J. Biol. Chem. 277:3168-3175(2002) [PubMed: 11698403] [Abstract] Cited for: HOMOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB093572 mRNA. Translation: BAC55010.1. AE013599 Genomic DNA. Translation: AAF58290.1. BT021295 mRNA. Translation: AAX33443.1. AY118651 mRNA. Translation: AAM50020.1. Different initiation.
RefSeq	NP_610931.1.
UniGene	Dm.30916
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-20580N.
IntAct	Q9V6X7. 4 interactions.
STRING	Q9V6X7.
Protein family/group databases
CAZy	GT65. Glycosyltransferase Family 65.
Proteomic databases
PRIDE	Q9V6X7.
Genome annotation databases
Ensembl	FBtr0087520; FBpp0086649; FBgn0033901; Drosophila melanogaster. [Genome view]
GeneID	36564.
KEGG	dme:Dmel_CG12366.
NMPDR	fig|7227.3.peg.5171.
Organism-specific databases
CTD	36564.
FlyBase	FBgn0033901. O-fut1.
Phylogenomic databases
eggNOG	inNOG07511.
InParanoid	Q9V6X7.
OMA	QSVYIAT.
OrthoDB	EOG9B8JMK.
PhylomeDB	Q9V6X7.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004551-MONOMER.
BRENDA	2.4.1.221. 48.
Gene expression databases
GermOnline	CG12366. Drosophila melanogaster.
Family and domain databases
InterPro	IPR019378. GDP-Fuc_prot_O-FucTrfase. [Graphical view]
Pfam	PF10250. O-FucT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	799227.

Entry information

Entry name

OFUT1_DROME

Accession

Primary (citable) accession number: Q9V6X7
Secondary accession number(s): Q5BIC9, Q86SA7, Q8MSR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project