Q9V6H2 (OR49B_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Odorant receptor 49b

Gene names

Name:	Or49b
Synonyms:	AN13
ORF Names:	CG17584

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Complexes with Orco to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Orco. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Orco complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the heteromeric odorant receptor channel (TC 1.A.69) family. [View classification]

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Non-traceable author statement Ref.4. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Non-traceable author statement Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Odorant receptor 49b		PRO_0000174253
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 49	21	Helical; Name=1; Potential
	Topological domain	50 – 60	11	Extracellular Potential
	Transmembrane	61 – 77	17	Helical; Name=2; Potential
	Topological domain	78 – 121	44	Cytoplasmic Potential
	Transmembrane	122 – 142	21	Helical; Name=3; Potential
	Topological domain	143 – 176	34	Extracellular Potential
	Transmembrane	177 – 197	21	Helical; Name=4; Potential
	Topological domain	198 – 251	54	Cytoplasmic Potential
	Transmembrane	252 – 272	21	Helical; Name=5; Potential
	Topological domain	273 – 278	6	Extracellular Potential
	Transmembrane	279 – 299	21	Helical; Name=6; Potential
	Topological domain	300 – 342	43	Cytoplasmic Potential
	Transmembrane	343 – 363	21	Helical; Name=7; Potential
	Topological domain	364 – 375	12	Extracellular Potential
Experimental info
	Sequence conflict	145 – 146	2	VL → GE Ref.4
	Sequence conflict	274	1	S → SRYVC Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q9V6H2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: DDC8728734B8B849	FASTA	375	43,899
	10 20 30 40 50 60 MFEDIQLIYM NIKILRFWAL LYDKNLRRYV CIGLASFHIF TQIVYMMSTN EGLTGIIRNS 70 80 90 100 110 120 YMLVLWINTV LRAYLLLADH DRYLALIQKL TEAYYDLLNL NDSYISEILD QVNKVGKLMA 130 140 150 160 170 180 RGNLFFGMLT SMGFGLYPLS SSERVLPFGS KIPGLNEYES PYYEMWYIFQ MLITPMGCCM 190 200 210 220 230 240 YIPYTSLIVG LIMFGIVRCK ALQHRLRQVA LKHPYGDRDP RELREEIIAC IRYQQSIIEY 250 260 270 280 290 300 MDHINELTTM MFLFELMAFS ALLCALLFML IIVSGTSQLI IVCMYINMIL AQILALYWYA 310 320 330 340 350 360 NELREQNLAV ATAAYETEWF TFDVPLRKNI LFMMMRAQRP AAILLGNIRP ITLELFQNLL 370 NTTYTFFTVL KRVYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Identification of candidate Drosophila olfactory receptors from genomic DNA sequence." Gao Q., Chess A. Genomics 60:31-39(1999) [PubMed: 10458908] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-342.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013599 Genomic DNA. Translation: AAF58452.1. BT024438 mRNA. Translation: ABC86500.1.
RefSeq	NP_523721.1. NM_078997.2.
UniGene	Dm.26920.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9V6H2.
Proteomic databases
PRIDE	Q9V6H2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0087781; FBpp0086894; FBgn0028963.
GeneID	36413.
KEGG	dme:Dmel_CG17584.
NMPDR	fig|7227.3.peg.4938.
Organism-specific databases
CTD	36413.
FlyBase	FBgn0028963. Or49b.
Phylogenomic databases
eggNOG	inNOG05706.
GeneTree	EMGT00050000009324.
InParanoid	Q9V6H2.
OMA	INMILAQ.
OrthoDB	EOG4SN04G.
PhylomeDB	Q9V6H2.
Gene expression databases
Bgee	Q9V6H2.
GermOnline	CG17584. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
KO	K08471.
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	798435.

Entry information

Entry name

OR49B_DROME

Accession

Primary (citable) accession number: Q9V6H2
Secondary accession number(s): Q29QF2, Q9U6X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project