Reviewed, UniProtKB/Swiss-Prot Q9V6A9 (OR49A_DROME)

Names and origin

Protein names

Recommended name:
    Putative odorant receptor 49a

Gene names

Name:	Or49a
ORF Names:	CG13158

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complexes with Or83b to form odorant-sensing units, providing sensitive and prolonged odorant signaling and calcium permeability. They are necessary and sufficient to promote functional reconstitution of odor-evoked signaling in sensory neurons that normally respond only to carbon dioxide By similarity.
Subunit structure	Interacts with Or83b. Complexes exist early in the endomembrane system in olfactory sensory neurons (OSNs), coupling these complexes to the conserved ciliary trafficking pathway By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Miscellaneous	The atypical heteromeric and topological design of the odorant receptors appears to be an insect-specific solution for odor recognition, making the OR/Or83b complex an attractive target for the development of highly selective insect repellents to disrupt olfactory-mediated host-seeking behaviors of insect disease vectors. Odor-evoked OR currents are independent of known G-protein-coupled second messenger pathways.
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Non-traceable author statement. Source: UniProtKB detection of chemical stimulus involved in sensory perception of smell Inferred from mutant phenotype. Source: FlyBase olfactory behavior Inferred from mutant phenotype. Source: FlyBase
Cellular component	integral to membrane Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	odorant binding Inferred from electronic annotation. Source: InterPro olfactory receptor activity Non-traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Mhc	P05661	1	EBI-183068,EBI-195031

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Putative odorant receptor 49a		PRO_0000174252
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	1 Potential
	Topological domain	28 – 34	7	Extracellular Potential
	Transmembrane	35 – 55	21	2 Potential
	Topological domain	56 – 70	15	Cytoplasmic Potential
	Transmembrane	71 – 91	21	3 Potential
	Topological domain	92 – 141	50	Extracellular Potential
	Transmembrane	142 – 162	21	4 Potential
	Topological domain	163 – 209	47	Cytoplasmic Potential
	Transmembrane	210 – 230	21	5 Potential
	Topological domain	231 – 266	36	Extracellular Potential
	Transmembrane	267 – 287	21	6 Potential
	Topological domain	288 – 296	9	Cytoplasmic Potential
	Transmembrane	297 – 317	21	7 Potential
	Topological domain	318 – 396	79	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9V6A9-1 [UniParc]. Last modified August 2, 2002. Version 3. Checksum: DA02277581D46C76	FASTA	396	46,463
	10 20 30 40 50 60 MEKLRSYEDF IFMANMMFKT LGYDLFHTPK PWWRYLLVRG YFVLCTISNF YEASMVTTRI 70 80 90 100 110 120 IEWESLAGSP SKIMRQGLHF FYMLSSQLKF ITFMINRKRL LQLSHRLKEL YPHKEQNQRK 130 140 150 160 170 180 YEVNKYYLSC STRNVLYVYY FVMVVMALEP LVQSCIMYLI GFGKADFTYK RIFPTRLTFD 190 200 210 220 230 240 SEKPLGYVLA YVIDFTYSQF IVNVSLGTDL WMMCVSSQIS MHLGYLANML ASIRPSPETE 250 260 270 280 290 300 QQDCDFLASI IKRHQLMIRL QKDVNYVFGL LLASNLFTTS CLLCCMAYYT VVEGFNWEGI 310 320 330 340 350 360 SYMMLFASVA AQFYVVSSHG QMLIDLSTNL AKAAFESKWY EGSLRYKKEI LILMAQAQRP 370 380 390 LEISARGVII ISLDTFKILM TITYRFFAVI RQTVEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF58519.3.
RefSeq	NP_523711.3.
UniGene	Dm.26912
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:21103N.
IntAct	Q9V6A9. 1 interaction.
Genome annotation databases
Ensembl	FBgn0033727. Drosophila melanogaster. [Contig view]
GeneID	36350.
KEGG	dme:Dmel_CG13158.
NMPDR	fig|7227.3.peg.4829.
Organism-specific databases
FlyBase	FBgn0033727. Or49a.
Phylogenomic databases
HOGENOM	Q9V6A9.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-004217-MON.
Gene expression databases
ArrayExpress	Q9V6A9.
GermOnline	CG13158. Drosophila melanogaster.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	798072.

Entry information

Entry name

OR49A_DROME

Accession

Primary (citable) accession number: Q9V6A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 2, 2002
Last modified:	June 16, 2009
This is version 67 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project