Reviewed, UniProtKB/Swiss-Prot Q9V5L3 (C49A1_DROME)

Names and origin

Protein names

Recommended name:
    Probable cytochrome P450 49a1
    EC=1.14.-.-
Alternative name(s):
    CYPXLIXA1

Gene names

Name:	Cyp49a1
ORF Names:	CG18377

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein Potential. Microsome membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AAM27517.1 differs from that shown. Reason: Miscellaneous discrepancy. Intron retention.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9V5L3-1)

Also known as: D;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: Q9V5L3-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-174: Missing.
     175-177: MPH → MEL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Probable cytochrome P450 49a1		PRO_0000051996
Sites
	Metal binding	536	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	1 – 174	174	Missing in isoform C.		VSP_000616
	Alternative sequence	175 – 177	3	MPH → MEL in isoform C.		VSP_000617
Experimental info
	Sequence conflict	461	1	S → N in AAL68266. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (D) [UniParc]. Last modified August 2, 2002. Version 3. Checksum: A0DE2D1118807817	FASTA	589	65,902
	10 20 30 40 50 60 MSGLRKTSIA LMRRSTSSTT ILPHSGGVGG AVSPPSSGVG VATEIEKSIA MQRLRTGESS 70 80 90 100 110 120 NPKKLNVSQQ PVTSVATTRT TASSLPAETT SSPAAAVRPY SEVPGPYPLP LIGNSWRFAP 130 140 150 160 170 180 LIGTYKISDL DKVMNELHVN YGKMAKVGGL IGHPDLLFVF DGDEIRNIFK KEEAMPHRPS 190 200 210 220 230 240 MPSLRHYKGD LRRDFFGDVA GLIGVHGPKW EAFRQEVQHI LLQPQTAKKY IPPLNDIASE 250 260 270 280 290 300 FMGRIELMRD EKDELPANFL HELYKWALES VGRVSLDTRL GCLSPEGSEE AQQIIEAINT 310 320 330 340 350 360 FFWAVPELEL RMPLWRIYPT KAYRSFVKAL DQFTAICMKN IGKTMDKADA DEARGLSKSE 370 380 390 400 410 420 ADISIVERIV RKTGNRKLAA ILALDLFLVG VDTTSVAASS TIYQLAKNPD KQKKLFDELQ 430 440 450 460 470 480 KVFPHREADI NQNVLEQMPY LRACVKETLR MRPVVIANGR SLQSDAVING YHVPKGTHVI 490 500 510 520 530 540 FPHLVVSNDP AYFPEPKRFL PERWLKQSTD AAGCPHANQK IHPFVSLPFG FGRRMCVGRR 550 560 570 580 FAEIELHTLL AKIFRKYKVS YNSGEFVYRV NSTYIPQSPL NFKLTLRDE
	Isoform C. Checksum: AB12EB38715FD0D5	FASTA	415	47,476
	10 20 30 40 50 60 MELRPSMPSL RHYKGDLRRD FFGDVAGLIG VHGPKWEAFR QEVQHILLQP QTAKKYIPPL 70 80 90 100 110 120 NDIASEFMGR IELMRDEKDE LPANFLHELY KWALESVGRV SLDTRLGCLS PEGSEEAQQI 130 140 150 160 170 180 IEAINTFFWA VPELELRMPL WRIYPTKAYR SFVKALDQFT AICMKNIGKT MDKADADEAR 190 200 210 220 230 240 GLSKSEADIS IVERIVRKTG NRKLAAILAL DLFLVGVDTT SVAASSTIYQ LAKNPDKQKK 250 260 270 280 290 300 LFDELQKVFP HREADINQNV LEQMPYLRAC VKETLRMRPV VIANGRSLQS DAVINGYHVP 310 320 330 340 350 360 KGTHVIFPHL VVSNDPAYFP EPKRFLPERW LKQSTDAAGC PHANQKIHPF VSLPFGFGRR 370 380 390 400 410 MCVGRRFAEI ELHTLLAKIF RKYKVSYNSG EFVYRVNSTY IPQSPLNFKL TLRDE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND C). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF58791.3. AE013599 Genomic DNA. Translation: AAF58793.2. AE013599 Genomic DNA. Translation: AAM68760.2. AY075453 mRNA. Translation: AAL68266.1. AY102688 mRNA. Translation: AAM27517.1. Sequence problems.
RefSeq	NP_610588.2. NP_724937.1. NP_995803.1.
UniGene	Dm.5498
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22876N.
IntAct	Q9V5L3. 1 interaction.
Genome annotation databases
Ensembl	FBgn0033524. Drosophila melanogaster. [Contig view]
GeneID	36105.
KEGG	dme:Dmel_CG18377.
Organism-specific databases
FlyBase	FBgn0033524. Cyp49a1.
Phylogenomic databases
HOGENOM	Q9V5L3.
OMA	Q9V5L3. YKWALES.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-003863-MON.
Gene expression databases
ArrayExpress	Q9V5L3.
GermOnline	CG18377. Drosophila melanogaster.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	796892.

Entry information

Entry name

C49A1_DROME

Accession

Primary (citable) accession number: Q9V5L3
Secondary accession number(s): A4UZC2, Q8MZH1, Q8SY18

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	August 2, 2002
Last modified:	June 16, 2009
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project