Reviewed, UniProtKB/Swiss-Prot Q9V576 (WUN_DROME)

Names and origin

Protein names

Recommended name:
    Putative phosphatidate phosphatase
    EC=3.1.3.4
Alternative name(s):
    Phosphatidic acid phosphatase type 2
    Protein wunen
    Germ cell guidance factor

Gene names

Name:	wun
ORF Names:	CG8804

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for guiding the germ cells early in the process of migration from the lumen of the developing gut towards the overlying mesoderm, where the germ cells enter the gonads. May be involved in lipid metabolism. Ref.1 Ref.7
Catalytic activity	A 3-sn-phosphatidate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Subunit structure	Homodimer. This complex seems not to be involved in substrate recognition, it may confer only structural or functional stability. Ref.8
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in embryonic gut in a pattern that guides germ cells towards mesoderm (initially in hindgut and then on lower side of gut). During extended germ band stage, expressed in ectoderm as a medial band throughout the trunk. Ref.1
Sequence similarities	Belongs to the PA-phosphatase related phosphoesterase family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Developmental protein Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dephosphorylation Non-traceable author statement. Source: UniProtKB embryonic pattern specification Traceable author statement. Source: FlyBase germ cell programmed cell death Traceable author statement. Source: FlyBase germ cell repulsion Traceable author statement. Source: FlyBase pole cell migration Traceable author statement. Source: FlyBase
Cellular component	integral to membrane Non-traceable author statement. Source: UniProtKB
Molecular function	phosphatidate phosphatase activity Non-traceable author statement. Source: UniProtKB protein homodimerization activity Traceable author statement. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9V576-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9V576-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Putative phosphatidate phosphatase		PRO_0000220917
Regions
	Transmembrane	90 – 110	21	Potential
	Transmembrane	138 – 158	21	Potential
	Transmembrane	266 – 286	21	Potential
	Transmembrane	330 – 350	21	Potential
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
	Glycosylation	169	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 79	79	Missing in isoform Short.		VSP_005084
Experimental info
	Mutagenesis	327	1	D → T: Loss of catalytic activity and dimerization. Ref.8
	Sequence conflict	152	1	F → L in AAC47449. Ref.4
	Sequence conflict	360	1	A → G in AAC47449. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 27, 2003. Version 2. Checksum: AD3C4F06B3DD9ADA	FASTA	379	42,789
	10 20 30 40 50 60 MPAVKIIMST ETSASETTPL RRSENETPDH KELAQSNSNS RQTTVNSNNN NYSNSVQVRL 70 80 90 100 110 120 QEQDRDSDSE QQQHTATITM DTNKRILCRV GLDVLILLCA GFPILLFFLL GEPYKRGFFC 130 140 150 160 170 180 DDESLKHPFH DSTVRNWMLY FIGAVIPVGV IFIVEVIISQ NKAKQDNGNA TSRRYVFMNY 190 200 210 220 230 240 ELPDWMIECY KKIGIYAFGA VLSQLTTDIA KYSIGRLRPH FIAVCQPQMA DGSTCDDAIN 250 260 270 280 290 300 AGKYIQEFTC KGVGSSARML KEMRLSFPSG HSSFTFFAMV YLALYLQARM TWRGSKLLRH 310 320 330 340 350 360 LLQFLFIMVA WYTALSRVSD YKHHWSDVLA GSLIGSISAL VVANYVSDLF QKPNTKPYLA 370 RTVQDMNASP AQAITITTN
	Isoform Short. Checksum: 9ABAB4CA898239A1	FASTA	300	33,929
	10 20 30 40 50 60 MDTNKRILCR VGLDVLILLC AGFPILLFFL LGEPYKRGFF CDDESLKHPF HDSTVRNWML 70 80 90 100 110 120 YFIGAVIPVG VIFIVEVIIS QNKAKQDNGN ATSRRYVFMN YELPDWMIEC YKKIGIYAFG 130 140 150 160 170 180 AVLSQLTTDI AKYSIGRLRP HFIAVCQPQM ADGSTCDDAI NAGKYIQEFT CKGVGSSARM 190 200 210 220 230 240 LKEMRLSFPS GHSSFTFFAM VYLALYLQAR MTWRGSKLLR HLLQFLFIMV AWYTALSRVS 250 260 270 280 290 300 DYKHHWSDVL AGSLIGSISA LVVANYVSDL FQKPNTKPYL ARTVQDMNAS PAQAITITTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila protein Wunen repels migrating germ cells." Zhang N., Zhang J.P., Purcell K.J., Chen Y., Howard K. Nature 385:64-67(1997) [PubMed: 8985246] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), FUNCTION, TISSUE SPECIFICITY.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila complementary DNA resource." Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M., Harvey D.A. Science 287:2222-2224(2000) [PubMed: 10731138] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Strain: Berkeley. Tissue: Head.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: Berkeley. Tissue: Embryo.
[6]	"Efficient functional dissection of enhancer sequences in Drosophila: a novel P- and hobo-based construct acting as an enhancer-capture element." Russell C., Bartos S., Phillips R.G., Whittle R. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-99.
[7]	"Fly and mammalian lipid phosphate phosphatase isoforms differ in activity both in vitro and in vivo." Burnett C., Howard K. EMBO Rep. 4:793-799(2003) [PubMed: 12856002] [Abstract] Cited for: FUNCTION.
[8]	"Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity." Burnett C., Makridou P., Hewlett L., Howard K. BMC Biochem. 5:2-2(2004) [PubMed: 14725715] [Abstract] Cited for: SUBUNIT, MUTAGENESIS OF ASP-327.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U73822 mRNA. Translation: AAC47449.1. AE013599 Genomic DNA. Translation: AAF58942.2. AE013599 Genomic DNA. Translation: AAM71066.1. AF145595 mRNA. Translation: AAD38570.1. BT001729 mRNA. Translation: AAN71484.1. AY046533 Genomic DNA. Translation: AAL34392.1.
RefSeq	NP_477193.1. NP_724787.1.
UniGene	Dm.3348
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0016078. Drosophila melanogaster. [Contig view]
GeneID	35966.
KEGG	dme:Dmel_CG8804.
Organism-specific databases
FlyBase	FBgn0016078. wun.
Phylogenomic databases
HOGENOM	Q9V576.
OMA	Q9V576. IVMILGE.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-003626-MON.
BRENDA	3.1.3.4. 48.
Gene expression databases
ArrayExpress	Q9V576.
GermOnline	CG8804. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016118. P_Acid_Pase/Cl_peroxidase_N. IPR000326. P_Acid_Pase_2/haloperoxidase. [Graphical view]
Gene3D	G3DSA:1.20.144.10. P_Acid_Pase/Cl_peroxidase_N. 1 hit.
Pfam	PF01569. PAP2. 1 hit. [Graphical view]
SMART	SM00014. acidPPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	796084.

Entry information

Entry name

WUN_DROME

Accession

Primary (citable) accession number: Q9V576
Secondary accession number(s): P91661 , Q8MKU5, Q8WT60, Q9U9Y7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	January 27, 2003
Last modified:	June 16, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project