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Protein

RNA-binding protein 8A

Gene

tsu

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).5 Publications

Miscellaneous

'Tsunagi' means 'connection' or 'link' in Japanese.1 Publication

GO - Molecular functioni

  • mRNA binding Source: FlyBase

GO - Biological processi

  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • establishment of pole plasm mRNA localization Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • microtubule cytoskeleton organization Source: FlyBase
  • mRNA export from nucleus Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • negative regulation of transposition, DNA-mediated Source: FlyBase
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: GO_Central
  • oocyte anterior/posterior axis specification Source: FlyBase
  • oocyte dorsal/ventral axis specification Source: FlyBase
  • oocyte nucleus migration involved in oocyte dorsal/ventral axis specification Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
  • regulation of pole plasm oskar mRNA localization Source: FlyBase
  • RNA splicing Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, RNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72187. mRNA 3'-end processing.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 8A
Alternative name(s):
Protein tsunagi
Gene namesi
Name:tsuImported
Synonyms:Y141 Publication
ORF Names:CG8781Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0033378. tsu.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Part of the EJC assembled on mRNAs in the nucleus and remains part of the complex when mRNA moves into the cytoplasm.

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • exon-exon junction complex Source: FlyBase
  • nucleus Source: FlyBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Developing photoreceptor cell clusters express reduced levels of rolled/MAPK and fail to differentiate normally.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000817651 – 165RNA-binding protein 8AAdd BLAST165

Proteomic databases

PaxDbiQ9V535.
PRIDEiQ9V535.

Expressioni

Developmental stagei

Expressed throughout development. Localizes to the posterior pole of the oocyte during stages 1-9 of oogenesis.1 Publication

Gene expression databases

BgeeiFBgn0033378.
GenevisibleiQ9V535. DM.

Interactioni

Subunit structurei

Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
magoP490286EBI-172458,EBI-159609

Protein-protein interaction databases

BioGridi61762. 4 interactors.
DIPiDIP-24075N.
IntActiQ9V535. 2 interactors.
MINTiMINT-816263.
STRINGi7227.FBpp0087648.

Structurei

Secondary structure

1165
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 28Combined sources12
Beta strandi32 – 35Combined sources4
Helixi36 – 38Combined sources3
Beta strandi72 – 79Combined sources8
Helixi86 – 93Combined sources8
Helixi94 – 96Combined sources3
Beta strandi99 – 103Combined sources5
Turni108 – 110Combined sources3
Beta strandi111 – 113Combined sources3
Beta strandi115 – 123Combined sources9
Helixi124 – 134Combined sources11
Beta strandi145 – 153Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HL6X-ray2.50A/C1-165[»]
1OO0X-ray1.85B47-156[»]
1RK8X-ray1.90A1-165[»]
2X1GX-ray3.35A/C1-165[»]
ProteinModelPortaliQ9V535.
SMRiQ9V535.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V535.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 151RRMPROSITE-ProRule annotationAdd BLAST79

Domaini

The RNA recognition motif (RRM) is involved in the interaction with mago. The RNA-binding activity of such domains is therefore uncertain.1 Publication

Sequence similaritiesi

Belongs to the RBM8A family.Curated

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
GeneTreeiENSGT00730000111185.
InParanoidiQ9V535.
KOiK12876.
OMAiYDTVEQD.
OrthoDBiEOG091G0PLI.
PhylomeDBiQ9V535.

Family and domain databases

CDDicd12324. RRM_RBM8. 1 hit.
InterProiView protein in InterPro
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
IPR033744. RRM_RBM8.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
PRINTSiPR01738. RNABINDINGM8.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVLDIDNA EEFEVDEDGD QGIVRLKEKA KHRKGRGFGS DSNTREAIHS
60 70 80 90 100
YERVRNEDDD ELEPGPQRSV EGWILFVTSI HEEAQEDEIQ EKFCDYGEIK
110 120 130 140 150
NIHLNLDRRT GFSKGYALVE YETHKQALAA KEALNGAEIM GQTIQVDWCF
160
VKGPKRVKKS EKRRR
Length:165
Mass (Da):19,010
Last modified:May 1, 2000 - v1
Checksum:iA47BE9ED37CCA045
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86E → G in AAL29185 (PubMed:11691839).Curated1
Sequence conflicti101N → S in AAL48627 (PubMed:12537569).Curated1
Sequence conflicti135N → R in AAL29185 (PubMed:11691839).Curated1
Sequence conflicti159K → R in AAL48627 (PubMed:12537569).Curated1
Sequence conflicti162K → M in AAL29185 (PubMed:11691839).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173550 mRNA. Translation: AAL29185.1.
AE013599 Genomic DNA. Translation: AAF58987.1.
AY071005 mRNA. Translation: AAL48627.1.
RefSeqiNP_610454.2. NM_136610.4.
UniGeneiDm.19884.

Genome annotation databases

EnsemblMetazoaiFBtr0088567; FBpp0087648; FBgn0033378.
GeneIDi35924.
KEGGidme:Dmel_CG8781.
UCSCiCG8781-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiRBM8A_DROME
AccessioniPrimary (citable) accession number: Q9V535
Secondary accession number(s): Q8SZA6, Q95X08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families