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Protein

RNA-binding protein 8A

Gene

tsu

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs (PubMed:14973490, PubMed:24967911). Involved in exon definition of genes containing long introns, including the rolled/MAPK gene (PubMed:20946982, PubMed:20946983). The mago-tsu heterodimer interacts with the EJC key regulator Pym leading to EJC disassembly in the cytoplasm (PubMed:24967911). Has a role in oskar mRNA localization to the posterior pole of the developing oocyte (PubMed:11691839).5 Publications

GO - Molecular functioni

  • mRNA binding Source: FlyBase
  • nucleotide binding Source: InterPro

GO - Biological processi

  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • establishment of pole plasm mRNA localization Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • microtubule cytoskeleton organization Source: FlyBase
  • mRNA export from nucleus Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • negative regulation of transposition, DNA-mediated Source: FlyBase
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: GO_Central
  • oocyte anterior/posterior axis specification Source: FlyBase
  • oocyte dorsal/ventral axis specification Source: FlyBase
  • oocyte nucleus migration involved in oocyte dorsal/ventral axis specification Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
  • regulation of pole plasm oskar mRNA localization Source: FlyBase
  • RNA splicing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

mRNA processing, mRNA splicing, mRNA transport, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72187. mRNA 3'-end processing.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 8A
Alternative name(s):
Protein tsunagi
Gene namesi
Name:tsuImported
Synonyms:Y141 Publication
ORF Names:CG8781Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0033378. tsu.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Part of the EJC assembled on mRNAs in the nucleus and remains part of the complex when mRNA moves into the cytoplasm.

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • exon-exon junction complex Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Developing photoreceptor cell clusters express reduced levels of rolled/MAPK and fail to differentiate normally.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 165165RNA-binding protein 8APRO_0000081765Add
BLAST

Proteomic databases

PaxDbiQ9V535.
PRIDEiQ9V535.

Expressioni

Developmental stagei

Expressed throughout development. Localizes to the posterior pole of the oocyte during stages 1-9 of oogenesis.1 Publication

Gene expression databases

BgeeiFBgn0033378.
GenevisibleiQ9V535. DM.

Interactioni

Subunit structurei

Heterodimer (via RRM domain) with mago (PubMed:11691839, PubMed:12730685, PubMed:12704080). Part of the mRNA splicing-dependent exon junction complex (EJC) complex; the core complex contains btz/CASC3, eIF4AIII, mago and tsu/RBM8A (PubMed:14973490). Interacts with Pym (via N-terminus); the interaction is direct (PubMed:24967911, PubMed:14968132). Interacts with eIF4AIII (PubMed:14973490).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
magoP490286EBI-172458,EBI-159609

Protein-protein interaction databases

BioGridi61762. 4 interactions.
DIPiDIP-24075N.
IntActiQ9V535. 2 interactions.
MINTiMINT-816263.
STRINGi7227.FBpp0087648.

Structurei

Secondary structure

1
165
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi17 – 2812Combined sources
Beta strandi32 – 354Combined sources
Helixi36 – 383Combined sources
Beta strandi72 – 798Combined sources
Helixi86 – 938Combined sources
Helixi94 – 963Combined sources
Beta strandi99 – 1035Combined sources
Turni108 – 1103Combined sources
Beta strandi111 – 1133Combined sources
Beta strandi115 – 1239Combined sources
Helixi124 – 13411Combined sources
Beta strandi145 – 1539Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HL6X-ray2.50A/C1-165[»]
1OO0X-ray1.85B47-156[»]
1RK8X-ray1.90A1-165[»]
2X1GX-ray3.35A/C1-165[»]
ProteinModelPortaliQ9V535.
SMRiQ9V535. Positions 10-155.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9V535.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 15179RRMPROSITE-ProRule annotationAdd
BLAST

Domaini

The RNA recognition motif (RRM) is involved in the interaction with mago. The RNA-binding activity of such domains is therefore uncertain.1 Publication

Sequence similaritiesi

Belongs to the RBM8A family.Curated
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
GeneTreeiENSGT00730000111185.
InParanoidiQ9V535.
KOiK12876.
OMAiISVDWGF.
OrthoDBiEOG091G0PLI.
PhylomeDBiQ9V535.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF72. PTHR23139:SF72. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR01738. RNABINDINGM8.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9V535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADVLDIDNA EEFEVDEDGD QGIVRLKEKA KHRKGRGFGS DSNTREAIHS
60 70 80 90 100
YERVRNEDDD ELEPGPQRSV EGWILFVTSI HEEAQEDEIQ EKFCDYGEIK
110 120 130 140 150
NIHLNLDRRT GFSKGYALVE YETHKQALAA KEALNGAEIM GQTIQVDWCF
160
VKGPKRVKKS EKRRR
Length:165
Mass (Da):19,010
Last modified:May 1, 2000 - v1
Checksum:iA47BE9ED37CCA045
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861E → G in AAL29185 (PubMed:11691839).Curated
Sequence conflicti101 – 1011N → S in AAL48627 (PubMed:12537569).Curated
Sequence conflicti135 – 1351N → R in AAL29185 (PubMed:11691839).Curated
Sequence conflicti159 – 1591K → R in AAL48627 (PubMed:12537569).Curated
Sequence conflicti162 – 1621K → M in AAL29185 (PubMed:11691839).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173550 mRNA. Translation: AAL29185.1.
AE013599 Genomic DNA. Translation: AAF58987.1.
AY071005 mRNA. Translation: AAL48627.1.
RefSeqiNP_610454.2. NM_136610.4.
UniGeneiDm.19884.

Genome annotation databases

EnsemblMetazoaiFBtr0088567; FBpp0087648; FBgn0033378.
GeneIDi35924.
KEGGidme:Dmel_CG8781.
UCSCiCG8781-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF173550 mRNA. Translation: AAL29185.1.
AE013599 Genomic DNA. Translation: AAF58987.1.
AY071005 mRNA. Translation: AAL48627.1.
RefSeqiNP_610454.2. NM_136610.4.
UniGeneiDm.19884.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HL6X-ray2.50A/C1-165[»]
1OO0X-ray1.85B47-156[»]
1RK8X-ray1.90A1-165[»]
2X1GX-ray3.35A/C1-165[»]
ProteinModelPortaliQ9V535.
SMRiQ9V535. Positions 10-155.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61762. 4 interactions.
DIPiDIP-24075N.
IntActiQ9V535. 2 interactions.
MINTiMINT-816263.
STRINGi7227.FBpp0087648.

Proteomic databases

PaxDbiQ9V535.
PRIDEiQ9V535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088567; FBpp0087648; FBgn0033378.
GeneIDi35924.
KEGGidme:Dmel_CG8781.
UCSCiCG8781-RA. d. melanogaster.

Organism-specific databases

CTDi110124.
FlyBaseiFBgn0033378. tsu.

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
GeneTreeiENSGT00730000111185.
InParanoidiQ9V535.
KOiK12876.
OMAiISVDWGF.
OrthoDBiEOG091G0PLI.
PhylomeDBiQ9V535.

Enzyme and pathway databases

ReactomeiR-DME-109688. Cleavage of Growing Transcript in the Termination Region.
R-DME-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72187. mRNA 3'-end processing.
R-DME-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

EvolutionaryTraceiQ9V535.
GenomeRNAii35924.
PROiQ9V535.

Gene expression databases

BgeeiFBgn0033378.
GenevisibleiQ9V535. DM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF72. PTHR23139:SF72. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
PRINTSiPR01738. RNABINDINGM8.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM8A_DROME
AccessioniPrimary (citable) accession number: Q9V535
Secondary accession number(s): Q8SZA6, Q95X08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

'Tsunagi' means 'connection' or 'link' in Japanese.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.