Q9V4X2 (PGSC2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidoglycan-recognition protein SC2
EC=3.5.1.28

Gene names

Name:	PGRP-SC2
ORF Names:	CG14745

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Cofactor	Zinc By similarity.
Subcellular location	Secreted Potential.
Tissue specificity	Constitutively expressed at high level in gut, in addition to the induced expression in fat body. Ref.4
Induction	Strongly up-regulated by PGN from B.subtilis. Regulated by both imd/Relish and Toll pathways. Ref.4 Ref.5
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Immunity Innate immunity
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	immune response Inferred from sequence or structural similarity Ref.4. Source: FlyBase innate immune response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of innate immune response Inferred from direct assay PubMed 16518472. Source: FlyBase peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from sequence or structural similarity Ref.4PubMed 15199956. Source: FlyBase
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC peptidoglycan binding Inferred from sequence or structural similarity Ref.4. Source: FlyBase zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 184	164	Peptidoglycan-recognition protein SC2		PRO_0000023912
Sites
	Metal binding	51	1	Zinc By similarity
	Metal binding	85	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	167	1	Zinc By similarity
Amino acid modifications
	Disulfide bond	57 ↔ 63		By similarity
Natural variations
	Natural variant	7	1	I → V in strain: DI7, Draveil, KY024, KY038, Loua, Monty5, Tahiti and ZW141.
	Natural variant	24	1	I → V in strain: DI7, Draveil, KY024, KY038, Loua, Monty5 and ZW141.
	Natural variant	72	1	A → T in strain: KY024, KY038 and ZW141.
	Natural variant	171	1	N → H in strain: ZW141.

Sequences

	Sequence		Length	Mass (Da)
	Q9V4X2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 0F99D04914B07238	FASTA	184	19,829
	10 20 30 40 50 60 MANKALILLA VLFCAQAVLG VTIISKSEWG GRSATSKTSL ANYLSYAVIH HTAGNYCSTK 70 80 90 100 110 120 AACITQLQNI QAYHMDSLGW ADIGYNFLIG GDGNVYEGRG WNVMGAHATN WNSKSIGISF 130 140 150 160 170 180 LGNYNTNTLT SAQITAAKGL LSDAVSRGQI VSGYILYGHR QVGSTECPGT NIWNEIRTWS NWKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins." Jiggins F.M., Hurst G.D.D. J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster." Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D. Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[5]	"The Toll and Imd pathways are the major regulators of the immune response in Drosophila." De Gregorio E., Spellman P.T., Tzou P., Rubin G.M., Lemaitre B. EMBO J. 21:2568-2579(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ556612 Genomic DNA. Translation: CAD89177.1. AJ556613 Genomic DNA. Translation: CAD89178.1. AJ556614 Genomic DNA. Translation: CAD89179.1. AJ556615 Genomic DNA. Translation: CAD89180.1. AJ556616 Genomic DNA. Translation: CAD89181.1. AJ556617 Genomic DNA. Translation: CAD89182.1. AJ556618 Genomic DNA. Translation: CAD89183.1. AJ556619 Genomic DNA. Translation: CAD89184.1. AJ556620 Genomic DNA. Translation: CAD89185.1. AJ556621 Genomic DNA. Translation: CAD89186.1. AJ556622 Genomic DNA. Translation: CAD89187.1. AE013599 Genomic DNA. Translation: AAF59051.1.
RefSeq	NP_610410.1. NM_136566.1.
UniGene	Dm.23629.
3D structure databases
ProteinModelPortal	Q9V4X2.
SMR	Q9V4X2. Positions 23-184.
ModBase	Search...
Proteomic databases
PRIDE	Q9V4X2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0088709; FBpp0087788; FBgn0043575.
GeneID	35862.
KEGG	dme:Dmel_CG14745.
Organism-specific databases
CTD	35862.
FlyBase	FBgn0043575. PGRP-SC2.
Phylogenomic databases
eggNOG	COG5479.
GeneTree	ENSGT00390000016833.
InParanoid	Q9V4X2.
KO	K01446.
OMA	TISCTTE.
OrthoDB	EOG49KD6R.
PhylomeDB	Q9V4X2.
Gene expression databases
Bgee	Q9V4X2.
GermOnline	CG14745. Drosophila melanogaster.
Family and domain databases
Gene3D	3.40.80.10. 1 hit.
InterPro	IPR002502. Amidase_domain. IPR017331. Peptidoglycan_recognition. IPR015510. PGRP. IPR006619. PGRP_domain_met/bac. [Graphical view]
PANTHER	PTHR11022. PTHR11022. 1 hit.
Pfam	PF01510. Amidase_2. 1 hit. [Graphical view]
PIRSF	PIRSF037945. PGRPs. 1 hit.
SMART	SM00644. Ami_2. 1 hit. SM00701. PGRP. 1 hit. [Graphical view]
SUPFAM	SSF55846. Amidase_2. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	35862.
NextBio	795563.

Entry information

Entry name

PGSC2_DROME

Accession

Primary (citable) accession number: Q9V4X2
Secondary accession number(s): Q70PT2 , Q70PT3, Q70PT5, Q70PT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project