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Protein

Peptidoglycan-recognition protein SC2

Gene

PGRP-SC2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

N-acetylmuramyl-L-alanine amidase involved in innate immunity by degrading bacterial peptidoglycans (PGN). Probably plays a scavenger role by digesting biologically active PGN into biologically inactive fragments. Has no direct bacteriolytic activity (By similarity).By similarity

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi51 – 511ZincBy similarity
Metal bindingi85 – 851ZincBy similarity
Metal bindingi159 – 1591ZincBy similarity
Metal bindingi167 – 1671ZincBy similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: FlyBase
  2. peptidoglycan binding Source: FlyBase
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. immune response Source: FlyBase
  2. innate immune response Source: UniProtKB-KW
  3. negative regulation of innate immune response Source: FlyBase
  4. peptidoglycan catabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan-recognition protein SC2 (EC:3.5.1.28)
Gene namesi
Name:PGRP-SC2
ORF Names:CG14745
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0043575. PGRP-SC2.

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 184164Peptidoglycan-recognition protein SC2PRO_0000023912Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 63By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ9V4X2.

Expressioni

Tissue specificityi

Constitutively expressed at high level in gut, in addition to the induced expression in fat body.1 Publication

Inductioni

Strongly up-regulated by PGN from B.subtilis. Regulated by both imd/Relish and Toll pathways.2 Publications

Gene expression databases

BgeeiQ9V4X2.
ExpressionAtlasiQ9V4X2. differential.

Structurei

3D structure databases

ProteinModelPortaliQ9V4X2.
SMRiQ9V4X2. Positions 23-184.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5479.
GeneTreeiENSGT00390000016833.
InParanoidiQ9V4X2.
KOiK01446.
OMAiTAGNYCT.
OrthoDBiEOG757CZ5.
PhylomeDBiQ9V4X2.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9V4X2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANKALILLA VLFCAQAVLG VTIISKSEWG GRSATSKTSL ANYLSYAVIH
60 70 80 90 100
HTAGNYCSTK AACITQLQNI QAYHMDSLGW ADIGYNFLIG GDGNVYEGRG
110 120 130 140 150
WNVMGAHATN WNSKSIGISF LGNYNTNTLT SAQITAAKGL LSDAVSRGQI
160 170 180
VSGYILYGHR QVGSTECPGT NIWNEIRTWS NWKA
Length:184
Mass (Da):19,829
Last modified:May 1, 2000 - v1
Checksum:i0F99D04914B07238
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti7 – 71I → V in strain: DI7, Draveil, KY024, KY038, Loua, Monty5, Tahiti and ZW141.
Natural varianti24 – 241I → V in strain: DI7, Draveil, KY024, KY038, Loua, Monty5 and ZW141.
Natural varianti72 – 721A → T in strain: KY024, KY038 and ZW141.
Natural varianti171 – 1711N → H in strain: ZW141.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556612 Genomic DNA. Translation: CAD89177.1.
AJ556613 Genomic DNA. Translation: CAD89178.1.
AJ556614 Genomic DNA. Translation: CAD89179.1.
AJ556615 Genomic DNA. Translation: CAD89180.1.
AJ556616 Genomic DNA. Translation: CAD89181.1.
AJ556617 Genomic DNA. Translation: CAD89182.1.
AJ556618 Genomic DNA. Translation: CAD89183.1.
AJ556619 Genomic DNA. Translation: CAD89184.1.
AJ556620 Genomic DNA. Translation: CAD89185.1.
AJ556621 Genomic DNA. Translation: CAD89186.1.
AJ556622 Genomic DNA. Translation: CAD89187.1.
AE013599 Genomic DNA. Translation: AAF59051.1.
RefSeqiNP_610410.1. NM_136566.2.

Genome annotation databases

EnsemblMetazoaiFBtr0088709; FBpp0087788; FBgn0043575.
GeneIDi35862.
KEGGidme:Dmel_CG14745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ556612 Genomic DNA. Translation: CAD89177.1.
AJ556613 Genomic DNA. Translation: CAD89178.1.
AJ556614 Genomic DNA. Translation: CAD89179.1.
AJ556615 Genomic DNA. Translation: CAD89180.1.
AJ556616 Genomic DNA. Translation: CAD89181.1.
AJ556617 Genomic DNA. Translation: CAD89182.1.
AJ556618 Genomic DNA. Translation: CAD89183.1.
AJ556619 Genomic DNA. Translation: CAD89184.1.
AJ556620 Genomic DNA. Translation: CAD89185.1.
AJ556621 Genomic DNA. Translation: CAD89186.1.
AJ556622 Genomic DNA. Translation: CAD89187.1.
AE013599 Genomic DNA. Translation: AAF59051.1.
RefSeqiNP_610410.1. NM_136566.2.

3D structure databases

ProteinModelPortaliQ9V4X2.
SMRiQ9V4X2. Positions 23-184.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9V4X2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088709; FBpp0087788; FBgn0043575.
GeneIDi35862.
KEGGidme:Dmel_CG14745.

Organism-specific databases

CTDi35862.
FlyBaseiFBgn0043575. PGRP-SC2.

Phylogenomic databases

eggNOGiCOG5479.
GeneTreeiENSGT00390000016833.
InParanoidiQ9V4X2.
KOiK01446.
OMAiTAGNYCT.
OrthoDBiEOG757CZ5.
PhylomeDBiQ9V4X2.

Miscellaneous databases

GenomeRNAii35862.
NextBioi795563.

Gene expression databases

BgeeiQ9V4X2.
ExpressionAtlasiQ9V4X2. differential.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR017331. Peptidoglycan_recognition.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
PIRSFiPIRSF037945. PGRPs. 1 hit.
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The evolution of parasite recognition genes in the innate immune system: purifying selection on Drosophila melanogaster peptidoglycan recognition proteins."
    Jiggins F.M., Hurst G.D.D.
    J. Mol. Evol. 57:598-605(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DI7, Draveil, KY024, KY038, Loua, Monty5, P.bourg, S30, Tahiti, Texas and ZW141.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster."
    Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.
    Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  5. "The Toll and Imd pathways are the major regulators of the immune response in Drosophila."
    De Gregorio E., Spellman P.T., Tzou P., Rubin G.M., Lemaitre B.
    EMBO J. 21:2568-2579(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPGSC2_DROME
AccessioniPrimary (citable) accession number: Q9V4X2
Secondary accession number(s): Q70PT2
, Q70PT3, Q70PT5, Q70PT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.