Q9V4M2 (WECH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein wech

Alternative name(s):
Protein dappled

Gene names

Name:	wech
Synonyms:	dpld
ORF Names:	CG42396

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	832 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vital for larval development. Plays a role in tumor formation. A crucial component for the physical link between integrins and the cytoskeleton in the epidermal muscle attachment sites. Ref.5 Ref.7
Subunit structure	Interacts with the head domain of rhea and the kinase domain of Ilk.
Tissue specificity	Expressed ubiquitously in all epithelial cells during early stages of embryogenesis. Specifically expressed at epidermal muscle attachment site. Ref.5 Ref.7
Developmental stage	Expressed both maternally and zygotically throughout development. Ref.7
Disruption phenotype	Muscle detachment in late-stage-16/early-stage-17 embryos. Ref.7
Miscellaneous	'Wech' means 'detached' or 'gone' in German.
Sequence similarities	Contains 2 B box-type zinc fingers. Contains 5 NHL repeats.
Caution	Was originally termed dappled (Ref.5).
Sequence caution	The sequence AAL25460.1 differs from that shown. Reason: Frameshift at position 591.

Ontologies

Keywords
Biological process	Differentiation Myogenesis
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW instar larval development Inferred from mutant phenotype Ref.5. Source: UniProtKB muscle attachment Inferred from mutant phenotype Ref.7. Source: UniProtKB regulation of cell-cell adhesion mediated by integrin Inferred from physical interaction Ref.7. Source: FlyBase
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro muscle tendon junction Inferred from direct assay Ref.7. Source: FlyBase
Molecular function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 832	832	Protein wech		PRO_0000220369
Regions
	Repeat	537 – 580	44	NHL 1
	Repeat	584 – 627	44	NHL 2
	Repeat	631 – 674	44	NHL 3
	Repeat	680 – 722	43	NHL 4
	Repeat	727 – 770	44	NHL 5
	Zinc finger	118 – 163	46	B box-type 1
	Zinc finger	184 – 224	41	B box-type 2
Amino acid modifications
	Modified residue	107	1	Phosphoserine Ref.6
	Modified residue	470	1	Phosphoserine Ref.6
	Modified residue	475	1	Phosphoserine Ref.6
	Modified residue	506	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Q9V4M2 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: A5672AFC3FDE1FE1	FASTA	832	90,573
	10 20 30 40 50 60 MMELLSNNSV PQQMASSNAP SANNVAHSST ANGSGGGSVS SNASNSSERL LAGILESFPA 70 80 90 100 110 120 WDLNVGLLPN VGQSSPPRAD FFINNFLGGL DTHGDFSIGP IGSGARSNPK MSPESSNNSS 130 140 150 160 170 180 ISCGWCEVSA SIRCLECNEF MCNDCLREHR NSPLSSNHSI VSLPTPIGAS PTGGSSVNAQ 190 200 210 220 230 240 TPPSGNFICD IHNEMLRYVC DYCRKLVCQC CTLHEHKEHS YASIQSFMVG SKEKLEGAIE 250 260 270 280 290 300 SSQVGTRCIK SSIDKALAFI RLIERNCSEL SDNIRKAFRQ FIIAIEDRER FLLDFVEKLR 310 320 330 340 350 360 QRRLAILHDQ MAGLKSALAG LSETSDMLSK VADNACNMDQ IEIAMKLTNG QRQMEQFAGI 370 380 390 400 410 420 YKDLQPKQEV FAFAPPDYSL LQDIRNQGGV ILVDDKNLPI VSSSNGIVPS VSSVNAVAAA 430 440 450 460 470 480 SVGVVGGVAG VVGGVGVSNG LDLAFGMNMP NNPLSVASSS VRRPLLRDNS FRIPSPIMQP 490 500 510 520 530 540 RGGSACGMSS GMSSAALDWE LNGLRSSPGL HFSAPRTTQA IPGCMDLVKV RNSNALSLSF 550 560 570 580 590 600 ATEGHEDGQV SRPWGLCVDK MGHVLVSDRR NNRVQVFNPD GSLKFKFGRK GVGNGEFDLP 610 620 630 640 650 660 AGICVDVDNR IIVVDKDNHR VQIFTASGVF LLKFGSYGKE YGQFQYPWDV AVNSRRQIVV 670 680 690 700 710 720 TDSRNHRIQQ FDSEGRFIRQ IVFDNHGQTK GIASPRGVCY TPTGNIIVSD FDNHCLYLID 730 740 750 760 770 780 PDINDILSVK GHEGSGFHEF NRPSGLCCDD EGRIIVADSK NQRILVFNQN LDFMWDIEVR 790 800 810 820 830 PSINPLMPPT LDEKDRTCDV AIMPDGRIVF LIELSPDSKE GSNPYKRFVH VF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kapadia B., Kronmiller B., Li P.W., Liao G. , Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Identification of immune system and response genes, and novel mutations causing melanotic tumor formation in Drosophila melanogaster." Rodriguez A., Zhou Z., Tang M.L., Meller S., Chen J., Bellen H., Kimbrell D.A. Genetics 143:929-940(1996) [PubMed: 8725239] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-107; SER-470; SER-475 AND SER-506, MASS SPECTROMETRY. Tissue: Embryo.
[7]	"The NHL-domain protein Wech is crucial for the integrin-cytoskeleton link." Loeer B., Bauer R., Bornheim R., Grell J., Kremmer E., Kolanus W., Hoch M. Nat. Cell Biol. 10:422-428(2008) [PubMed: 18327251] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013599 Genomic DNA. Translation: AAF59246.2. AE013599 Genomic DNA. Translation: AAM68901.1. AE013599 Genomic DNA. Translation: ACL83068.1. AE013599 Genomic DNA. Translation: ACL83069.1. AY060421 mRNA. Translation: AAL25460.1. Frameshift. BT010087 mRNA. Translation: AAQ22556.1. BT030403 mRNA. Translation: ABO52822.1.
RefSeq	NP_001137614.1. NM_001144142.1. NP_001137615.1. NM_001144143.1. NP_524772.2. NM_080033.3. NP_724567.1. NM_165533.2. NP_724568.1. NM_165534.2.
UniGene	Dm.1824.
3D structure databases
ProteinModelPortal	Q9V4M2.
SMR	Q9V4M2. Positions 116-162, 184-226, 533-814.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-21516N.
MINT	MINT-1569933.
STRING	Q9V4M2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0300080; FBpp0289357; FBgn0259745. FBtr0300081; FBpp0289358; FBgn0259745. FBtr0300082; FBpp0289359; FBgn0259745. FBtr0300083; FBpp0289360; FBgn0259745. FBtr0300084; FBpp0289361; FBgn0259745.
GeneID	44653.
KEGG	dme:Dmel_CG42396.
Organism-specific databases
CTD	44653.
FlyBase	FBgn0259745. wech.
Phylogenomic databases
eggNOG	inNOG07507.
GeneTree	EMGT00050000000319.
OMA	ATEGHED.
OrthoDB	EOG4ZPCB5.
PhylomeDB	Q9V4M2.
Gene expression databases
ArrayExpress	Q9V4M2.
Bgee	Q9V4M2.
GermOnline	CG1624. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR001258. NHL_repeat. IPR013017. NHL_repeat_subgr. IPR000315. Znf_B-box. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
KO	K12035.
Pfam	PF01436. NHL. 5 hits. PF00643. zf-B_box. 2 hits. [Graphical view]
SMART	SM00336. BBOX. 2 hits. [Graphical view]
PROSITE	PS51125. NHL. 5 hits. PS50119. ZF_BBOX. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	837513.

Entry information

Entry name

WECH_DROME

Accession

Primary (citable) accession number: Q9V4M2
Secondary accession number(s): A4IJ45 , B7YZS8, Q0E9G5, Q95SY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project