Q9V4A7 (PLXB_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plexin-B

Gene names

Name:	plexB
ORF Names:	CG17245

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2051 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in peripheral and central nervous system axon guidance. Acts as a receptor for Sema-2a and seems to transduce signal by suppressing Rac activity and enhancing Rho activity. Ref.5 Ref.6
Subunit structure	Interacts with plexA. Interacts with Rho1 and with the active forms of Rac1 and Rac2. Interacts with Sema-2a. Ref.5 Ref.6
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Developmental stage	Highly expressed in the embryonic nervous system. Ref.1 Ref.6
Sequence similarities	Belongs to the plexin family. Contains 3 IPT/TIG domains. Contains 3 PSI domains. Contains 1 Sema domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Membrane
Domain	Coiled coil Repeat Signal Transmembrane Transmembrane helix
Molecular function	Developmental protein Receptor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	motor axon guidance Non-traceable author statement. Source: FlyBase negative regulation of Rac protein signal transduction Non-traceable author statement. Source: FlyBase positive regulation of Rho protein signal transduction Non-traceable author statement. Source: FlyBase signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction Ref.6. Source: UniProtKB receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 2051	2018	Plexin-B		PRO_0000248554
Regions
	Topological domain	34 – 1380	1347	Extracellular Potential
	Transmembrane	1381 – 1401	21	Helical; Potential
	Topological domain	1402 – 2051	650	Cytoplasmic Potential
	Domain	61 – 533	473	Sema
	Domain	535 – 587	53	PSI 1
	Domain	683 – 730	48	PSI 2
	Domain	834 – 880	47	PSI 3
	Domain	888 – 975	88	IPT/TIG 1
	Domain	977 – 1064	88	IPT/TIG 2
	Domain	1097 – 1193	97	IPT/TIG 3
	Region	1402 – 1784	383	Interaction with PlexA
	Region	1722 – 1728	7	Interaction with activated Rac1 and Rac2
	Region	1788 – 1827	40	Interaction with Rho1
	Coiled coil	1402 – 1451	50	Potential
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Potential
	Glycosylation	276	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Glycosylation	541	1	N-linked (GlcNAc...) Potential
	Glycosylation	863	1	N-linked (GlcNAc...) Potential
	Glycosylation	1053	1	N-linked (GlcNAc...) Potential
	Glycosylation	1092	1	N-linked (GlcNAc...) Potential
	Glycosylation	1140	1	N-linked (GlcNAc...) Potential
	Glycosylation	1181	1	N-linked (GlcNAc...) Potential
	Glycosylation	1307	1	N-linked (GlcNAc...) Potential
	Glycosylation	1316	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	1082	1	S → A in AAD09426. Ref.1
	Sequence conflict	1289	1	S → T in AAD09426. Ref.1
	Sequence conflict	1526	1	R → Q in AAD09426. Ref.1
	Sequence conflict	1685	1	R → K in AAD09426. Ref.1
	Sequence conflict	1691	1	V → L in AAD09426. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9V4A7 [UniParc]. Last modified October 1, 2002. Version 2. Checksum: B65A57D7A255B44D	FASTA	2,051	231,680
	10 20 30 40 50 60 MLRKELYCAN IIHVLHVLCI IIILGSQHYC VNCIEELPAQ ALSPLNDIVA QFNLPSIPVQ 70 80 90 100 110 120 STASSYGNRS IGNNIESVRD SQSKNYFTHM SFDFMHNVLF AGATNKILKL NENLRVLAEA 130 140 150 160 170 180 VTGPLHDSPQ CHAGGCPEDI ETSLVNNFNK ILVVSYAHDG ILIACGSIRQ GACEIYSLPR 190 200 210 220 230 240 FPATPQFFAV PLAANDENAS TYAFVGPARY AWKEEDILYV GTTFTNVGDY RHDVPAISSR 250 260 270 280 290 300 RLDDLNYAEF SIQQSIINID VKYRDHFLVD YIYGFNSSEY AYFIIVQKKS HLADEAGYVT 310 320 330 340 350 360 RLARICITDP NYDSYTEITV QCTATENNVD YNIVRDAKVT PASHKLAQKM GIKKDDHVLV 370 380 390 400 410 420 TVFSPSREIS NQPESKSAMC IYSIKDIEDM FIENIHLCFN GTTKDRNLGY ISGTINDGRC 430 440 450 460 470 480 PIVGSLGNIY NFCSVGLKIS GVSPITAHAL FHFDNVSVTS VTATSTTDQQ HSLAFLGTNM 490 500 510 520 530 540 GVIKKVLLSG QSPGEYEEIV VDAGNRILPN TMMSPKKDFL YVLSQRKITK LRIEHCSVYT 550 560 570 580 590 600 NCSACLESRD PFCGWCSLEK RCTVRSTCQR DTSASRWLSL GSGQQCIEFE SIIPEKIPIS 610 620 630 640 650 660 ELSHLHLIIR TLPEPFNAKY RCVFGNSTPI DAEILENGLG CATPPLDERP LIPTNTDHIL 670 680 690 700 710 720 VPLSVRSSET NKDFVSRNFA FFDCSHHGNC QECLQSSWGC NWCIFDNKCV HKSLQCRNIE 730 740 750 760 770 780 NAVSTVGHCP HLKKNRPEIL LPVRVPIEIR LEIENLPKPK SAHAGFLCTI HIEAAQMLLP 790 800 810 820 830 840 AHVESNKIVV CEKTPYFYEI NTHEYQAKVV VTWNFQHYVD TAIVTLYKCD VLGSHREHPD 850 860 870 880 890 900 CSLCVTRDPK YKCAWCSNSC VYNETCIADK NSISSGSKSA IENECPLPRI DIIKPLSGPV 910 920 930 940 950 960 EGGTLITIEG SNLGIREEDV RGKIFIGSVP CELENYQISV KIECRTGASL YEMSAPIKVA 970 980 990 1000 1010 1020 NDAGFTESSV QFHFKNVLLT GLYPTIGPRS GGTQLSLIGK FLNIGSTMRA FLDEYECHID 1030 1040 1050 1060 1070 1080 VTQASSSQVS CTTSEATQPE PIRSLHLVID GANRTLECQI STPSITNTNP TRSNFGSYQL 1090 1100 1110 1120 1130 1140 RSLPRQPCSI FNYTQDPRIM QIKPLRSFKS GGRVLTVHGI YLNSIQKPEL EVFYDNERVN 1150 1160 1170 1180 1190 1200 KTSCVVINSN QMECPSPPVN YKFETFKKTN RKMDTELHLQ NSSFQTETRY KNEYDRKKRR 1210 1220 1230 1240 1250 1260 ADFGDTFRLY TNAGSSANYF VNNNMDVTTF VKVHETQLNL QLSFVMDNVQ LVRNLNKYFH 1270 1280 1290 1300 1310 1320 DIRSTIVYLA DPKYLPFPND GVKLYKGDSL VIEGELLNLA ADEYDVNVTI GTSQCNITSL 1330 1340 1350 1360 1370 1380 ALNQLLCIPP EQQPLPTDEN GVDQSTDLPL VVVKVGRNLR FVIGYLKYDL NKPYVFSHAL 1390 1400 1410 1420 1430 1440 LVGILTVALL VVVFVIVLII FRRKSTQAER EYKRIQIQMI TLESNVRSEC KQAFAELQTD 1450 1460 1470 1480 1490 1500 MTDLTADLES TGIPTLDHVN YIMKVFFPGV SDHPILNSPK FREGSPQTNY DAAMVQFEQL 1510 1520 1530 1540 1550 1560 IGNKYFLLMF IETLEAQRSS FSIRDRVNVA SLIMVVLMNK MEYATEILKS LLLRLIDKSL 1570 1580 1590 1600 1610 1620 ASKHPQLMLR RTESVVEKML TNYLAICMYD YLKEYAGSNL FLLFKAIKHQ IEKGLVDAIT 1630 1640 1650 1660 1670 1680 NDARYSLSEE RLLHEQVTHS VVILHILQDD LDEKVQCRVN DWDTISQVKL KILDAIFKNT 1690 1700 1710 1720 1730 1740 PFSMRPSVNE VDLEWRHGRG GHLTLQDEDL TTKTVNGWKR LNTLAHYGVK ESAVMSLIAR 1750 1760 1770 1780 1790 1800 QNDNYHIPYS KNQNSAPYHN FYFINNSQSH IIINNDIESG LQQPRVYHLV KPNIPDHYMN 1810 1820 1830 1840 1850 1860 IKNSVLSGGS PAFQSHVVNN CNDRVNKTIP EVYLTRLLAT KGTIQKFVDD FFSIILTVNE 1870 1880 1890 1900 1910 1920 ELPPAVKWLF DLLDEAARRH DIADTDIVHA WKSNCLPLRF WVNFIKNPDF IFDVNKTYSV 1930 1940 1950 1960 1970 1980 DSCLSVIAQT FMDACSTSEH RLGKDSPSNK LLFAKDIPNY RIMVKEFYRD VSRLPQISDQ 1990 2000 2010 2020 2030 2040 EMSTAMQQLS VRQNEEFDTI SALKELYIYI TKYKDQIMES LETDINCRKM HLSRKLGNVA 2050 ATLDGDCTSN C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Plexin A is a neuronal semaphorin receptor that controls axon guidance." Winberg M.L., Noordermeer J.N., Tamagnone L., Comoglio P.M., Spriggs M.K., Tessier-Lavigne M., Goodman C.S. Cell 95:903-916(1998) [PubMed: 9875845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Plexin B mediates axon guidance in Drosophila by simultaneously inhibiting active Rac and enhancing RhoA signaling." Hu H., Marton T.F., Goodman C.S. Neuron 32:39-51(2001) [PubMed: 11604137] [Abstract] Cited for: FUNCTION, INTERACTION WITH RHO1; RAC1 AND RAC2.
[6]	"Drosophila Plexin B is a Sema-2a receptor required for axon guidance." Ayoob J.C., Terman J.R., Kolodkin A.L. Development 133:2125-2135(2006) [PubMed: 16672342] [Abstract] Cited for: FUNCTION, INTERACTION WITH PLEXA AND SEMA-2A, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF106933 mRNA. Translation: AAD09426.1. AE014135 Genomic DNA. Translation: AAF59374.2. BT010009 mRNA. Translation: AAQ22478.1.
PIR	T13164.
RefSeq	NP_524616.2. NM_079877.2.
UniGene	Dm.1700.
3D structure databases
HSSP	HSSP built from PDB template 1SHY based on UniProtKB P08581.
ProteinModelPortal	Q9V4A7.
SMR	Q9V4A7. Positions 85-589, 680-718, 824-976, 1404-2046.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9V4A7. 1 interaction.
MINT	MINT-843871.
STRING	Q9V4A7.
Proteomic databases
PRIDE	Q9V4A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089179; FBpp0088246; FBgn0025740. FBtr0308297; FBpp0300616; FBgn0025740.
GeneID	43766.
KEGG	dme:Dmel_CG17245.
NMPDR	fig|7227.3.peg.8326.
Organism-specific databases
CTD	43766.
FlyBase	FBgn0025740. plexB.
Phylogenomic databases
eggNOG	inNOG05135.
GeneTree	EMGT00050000012421.
InParanoid	Q9V4A7.
OMA	QMECPSP.
OrthoDB	EOG4K98SZ.
PhylomeDB	Q9V4A7.
Gene expression databases
Bgee	Q9V4A7.
GermOnline	CG17245. Drosophila melanogaster.
Family and domain databases
InterPro	IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002909. IPT_TIG_rcpt. IPR003659. Plexin-like. IPR016201. Plexin-like_fold. IPR013548. Plexin_cytoplasmic_RasGAP_dom. IPR002165. Plexin_repeat. IPR008936. Rho_GTPase_activation_prot. IPR001627. Semaphorin/CD100_Ag. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 4 hits. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K06821.
Pfam	PF08337. Plexin_cytopl. 1 hit. PF01437. PSI. 2 hits. PF01403. Sema. 1 hit. PF01833. TIG. 3 hits. [Graphical view]
SMART	SM00429. IPT. 3 hits. SM00423. PSI. 3 hits. SM00630. Sema. 1 hit. [Graphical view]
SUPFAM	SSF81296. Ig_E-set. 3 hits. SSF103575. Plexin-like_fold. 1 hit. SSF48350. Rho_GAP. 1 hit. SSF101912. Sema. 1 hit.
PROSITE	PS51004. SEMA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	835672.

Entry information

Entry name

PLXB_DROME

Accession

Primary (citable) accession number: Q9V4A7
Secondary accession number(s): O96682

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project