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Protein

DNA replication licensing factor Mcm6

Gene

Mcm6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity Required for DNA replication and cell proliferation. Required for mitotic cycles, endocycles, and the special S phase associated with the amplification of chorion genes; has a role in origin unwinding or fork elongation at chorion loci.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri152 – 179C4-typeSequence analysisAdd BLAST28
Nucleotide bindingi388 – 395ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • DNA replication Source: UniProtKB
  • DNA replication initiation Source: InterPro
  • eggshell chorion gene amplification Source: FlyBase
  • lateral inhibition Source: FlyBase
  • oogenesis Source: FlyBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, Cell division, DNA replication, Mitosis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-176187. Activation of ATR in response to replication stress.
R-DME-68867. Assembly of the pre-replicative complex.
R-DME-68949. Orc1 removal from chromatin.
R-DME-68962. Activation of the pre-replicative complex.
R-DME-69052. Switching of origins to a post-replicative state.
R-DME-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor Mcm6 (EC:3.6.4.12)
Short name:
DmMCM6
Gene namesi
Name:Mcm6Imported
ORF Names:CG4039
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0025815. Mcm6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157T → M in allele 4; homozygous lethal. 1 Publication1
Mutagenesisi388G → D in allele 5; homozygous lethal. 1 Publication1
Mutagenesisi394K → A: Slihgtly reduces complex helicase activity. 1 Publication1
Mutagenesisi676M → K in allele K1214; eggs exhibit thin shell and flimsy dorsal appendages. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002333151 – 817DNA replication licensing factor Mcm6Add BLAST817

Proteomic databases

PaxDbiQ9V461.
PRIDEiQ9V461.

Expressioni

Tissue specificityi

In stage 12 embryos, strongly expressed in the CNS and weakly in the gut.1 Publication

Developmental stagei

Expressed both maternally and zygotically.1 Publication

Gene expression databases

BgeeiFBgn0025815.
ExpressionAtlasiQ9V461. differential.
GenevisibleiQ9V461. DM.

Interactioni

Subunit structurei

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). The heterodimers of Mcm4/Mcm6 and Mcm3/Mcm5 interact with Mcm2 and Mcm7.Curated1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi58089. 10 interactors.
DIPiDIP-35347N.
IntActiQ9V461. 8 interactors.
STRINGi7227.FBpp0070913.

Structurei

3D structure databases

ProteinModelPortaliQ9V461.
SMRiQ9V461.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini338 – 544MCMSequence analysisAdd BLAST207

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi520 – 523Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Sequence analysis

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri152 – 179C4-typeSequence analysisAdd BLAST28

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00550000074860.
InParanoidiQ9V461.
KOiK02542.
OMAiQFLKYVC.
OrthoDBiEOG091G020Q.
PhylomeDBiQ9V461.

Family and domain databases

InterProiView protein in InterPro
IPR031327. MCM.
IPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTiView protein in SMART
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9V461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVADAQVGQ LRVKDEVGIR AQKLFQDFLE EFKEDGEIKY TRPAASLESP
60 70 80 90 100
DRCTLEVSFE DVEKYDQNLA TAIIEEYYHI YPFLCQSVSN YVKDRIGLKT
110 120 130 140 150
QKDCYVAFTE VPTRHKVRDL TTSKIGTLIR ISGQVVRTHP VHPELVSGVF
160 170 180 190 200
MCLDCQTEIR NVEQQFKFTN PTICRNPVCS NRKRFMLDVE KSLFLDFQKI
210 220 230 240 250
RIQETQAELP RGCIPRAVEI ILRSELVETV QAGDRYDFTG TLIVVPDVSV
260 270 280 290 300
LAGVGTRAEN SSRHKPGEGM DGVTGLKALG MRELNYRMAF LACSVQATTA
310 320 330 340 350
RFGGTDLPMS EVTAEDMKKQ MTDAEWHKIY EMSKDRNLYQ NLISSLFPSI
360 370 380 390 400
YGNDEVKRGI LLQQFGGVAK TTTEKTSLRG DINVCIVGDP STAKSQFLKQ
410 420 430 440 450
VSDFSPRAIY TSGKASSAAG LTAAVVRDEE SFDFVIEAGA LMLADNGICC
460 470 480 490 500
IDEFDKMDQR DQVAIHEAME QQTISIARAG VRATLNARTS ILAAANPING
510 520 530 540 550
RYDRSKSLQQ NIQLSAPIMS RFDLFFILVD ECNEVVDYAI ARKIVDLHSN
560 570 580 590 600
IEESVERAYT REEVLRYVTF ARQFKPVISQ EAGHMLVENY GHLRQRDTGT
610 620 630 640 650
SGRSTWRITV RQLESMIRLS EAMAKLECSN RVLERHVKEA FRLLNKSIIR
660 670 680 690 700
VEQPDIHLDD DEGLDMDDGI QHDIDMENNG AAANVDENNG MDTSASGAVQ
710 720 730 740 750
KKKFTLSFED YKNLSTMLVL HMRAEEARCE VEGNDTGIKR SNVVTWYLEQ
760 770 780 790 800
VADQIESEDE LISRKNLIEK LIDRLIYHDQ VIIPLKTSTL KPRIQVQKDF
810
VEEDDPLLVV HPNYVVE
Length:817
Mass (Da):92,353
Last modified:May 1, 2000 - v1
Checksum:i0B7921FB3D888980
GO

Sequence cautioni

The sequence AAK93526 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10 – 11QL → HV in BAA34732 (PubMed:9795205).Curated2
Sequence conflicti550N → K in BAA34732 (PubMed:9795205).Curated1
Sequence conflicti695A → G (PubMed:9795205).Curated1
Sequence conflicti695A → G (PubMed:12537569).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010108 mRNA. Translation: BAA34732.1.
AF124744 mRNA. Translation: AAD32858.1.
AE014298 Genomic DNA. Translation: AAF46184.1.
AY052102 mRNA. Translation: AAK93526.1. Different initiation.
RefSeqiNP_511065.1. NM_078510.3.
UniGeneiDm.2944.

Genome annotation databases

EnsemblMetazoaiFBtr0070952; FBpp0070913; FBgn0025815.
GeneIDi31603.
KEGGidme:Dmel_CG4039.

Similar proteinsi

Entry informationi

Entry nameiMCM6_DROME
AccessioniPrimary (citable) accession number: Q9V461
Secondary accession number(s): O96047, Q960E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 1, 2000
Last modified: October 25, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families